pycom02g20950

protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0019577
Physical Location & Seq
Forward (+)
19028828 .. 19030307
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20950.3

Sequence Viewer

Length: 1353 bp
ATGATGAAAGTTTTAGATTCGGTTGCATTTTGTGCCTTCCAGAATAGCAGGCAGGGGCAGATGTACAAGCCAACCACAACCCTTGATGAGGGCCGTGTAAATAGCTCCAAACTCAGCCTCTTCGAGTTCAAAGCTTCTGTATTATGCTCACAAGTGACCCCTTTTGGCCCCTTCAATTCCTTTGTACAAAAAAGATGGCTAAAGCCTGTAATTTCGGCGCGAACACGCTTAGAAGACCGAACAAGGGACTCGAAGCTAGACAATCTCACAACCCACCTCAAGAAGCTCGATATCATCCTAAAGCTCCATGACCTCATGTCCAGTCGGAAGCGCGGACCCTTTGTGTCCTTGCAACTGATGTCTCGATGGAGGAACCTTGTGGGGCTTAATGTAGGCATTGGCGCCTTTGTTCACAAATACCCACATGTTTTTGAGGTGTTTAAACATCCGAAAATGAGGGGTTTAATAGAGGAAGAGGCAATGGCTATGAAACAATGGGAACTCGAAGTTGTTCATCGTGTAAAGAAGTTGTTGATGATGTCTGTGAGTGGCACTCTGCGTGTCCATGCTTTGAGGTTGGTCAGGAGAGAATTTGGATTGCCCGAGGATTTCAGAGAATCTATCCTTGGTAAATATTCAAAGGATTTTAGGTTGATTGATTTAGAGGTTGTAGAACTGATTAGCAGAGATGAGAATTTGGATGTTGCTGAAATTGAGAAATGGCGAGAGAAGGAATTTAAAGAGAAGTGGTTGAGTGAGTTTGAGACAAAGTATGCCTTCCCTATAAATTTCCCAACAGGGTATGAGATCCAGGCAGGATTTAGAGATAAGTTGAAGAATTGGCAAAGGCTTCCTTACGTGAAGCCTTATGACAGGAAAGAGGTTGTCCGCATTCGTACTTGTGGAGGGATAGAGAGGTATGAGAAACGGGCGGTTGGTATTCTTCACGAGTTCTTGAGTTTGACAGTAGAGAAGATGGTTGAGGTTGAAAGATTATCTCATTTTTGGAGAGATTTTTCTATGGTCGTTAATGTGCGGGAGCTTATCTTGAAGCACCCTGGAATATTTTACATATCAACCAGACGTAATAGTCAAACTGTTTTTCTTAGAGAGGCTTATAGTAAAGGGTGCCTGATTGAGTCCAATCCGATATATGATGTTCGGAGGAAAACTTTAGACCTTGTCTTCCTAGGGCGTCGAAATACTAGACAAATGCCAGCTCCAAAGGAAATTAAGGAGTTCAAAGTAGATGGGGATGGCAAAATGGATGGTGACTGGGTTATTCCAATGTTAGAGAACTTGGACAATGGAATCCTTGAAACTAGTGAAATGGATATGTTGATCATTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

451

Amino Acids

52.98

Weight (kDa)

9.47

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45790 AT5G45790
malus_domestica MD02G1242700.v1.1 MD07G1073900.v1.1
prunus_persica Prupe.2G091800_v2.0.a1
pyrus_communis pycom02g20950 pycom07g05800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1089
AccB1I GGYRCC 2 cut(s) 401, 1128
AccII CGCG 2 cut(s) 220, 333
AciI CCGC 4 cut(s) 333, 889, 932, 1036
AclWI GGATC 1 cut(s) 802
AcsI RAATTY 4 cut(s) 590, 694, 734, 787
AcyI GRCGYC 2 cut(s) 402, 1195
AfaI GTAC 3 cut(s) 65, 186, 898
AfiI CCNNNNNNNGG 2 cut(s) 88, 244
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 8 cut(s) 130, 175, 639, 835, 989, 1051, 1243, 1319
AhdI GACNNNNNGTC 1 cut(s) 316
AhlI ACTAGT 1 cut(s) 1322
AjnI CCWGG 2 cut(s) 810, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 609, 641
AluBI AGCT 7 cut(s) 105, 134, 256, 286, 304, 1042, 1220
AluI AGCT 7 cut(s) 105, 134, 256, 286, 304, 1042, 1220
Alw26I GTCTC 2 cut(s) 366, 758
AlwI GGATC 1 cut(s) 802
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 2 cut(s) 91, 166
ApoI RAATTY 4 cut(s) 590, 694, 734, 787
Asp700I GAANNNNTTC 1 cut(s) 510
AspA2I CCTAGG 1 cut(s) 1189
AspLEI GCGC 3 cut(s) 220, 333, 404
AspS9I GGNCC 3 cut(s) 91, 167, 335
AsuHPI GGTGA 1 cut(s) 1283
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 1 cut(s) 335
AvrII CCTAGG 1 cut(s) 1189
BanI GGYRCC 2 cut(s) 401, 1128
BauI CACGAG 1 cut(s) 947
BbsI GAAGAC 2 cut(s) 240, 1177
BccI CCATC 6 cut(s) 189, 360, 970, 1244, 1250, 1262
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 2 cut(s) 812, 1059
BclI TGATCA 1 cut(s) 1341
BcoDI GTCTC 2 cut(s) 366, 758
BcuI ACTAGT 1 cut(s) 1322
BfaI CTAG 4 cut(s) 257, 1190, 1206, 1323
BfoI RGCGCY 1 cut(s) 405
BlnI CCTAGG 1 cut(s) 1189
Bme1390I CCNGG 2 cut(s) 812, 1059
Bme18I GGWCC 1 cut(s) 335
BmeRI GACNNNNNGTC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 3 cut(s) 91, 167, 335
BmiI GGNNCC 5 cut(s) 169, 337, 374, 403, 1130
BmrFI CCNGG 2 cut(s) 812, 1059
BmrI ACTGGG 1 cut(s) 1285
BmuI ACTGGG 1 cut(s) 1285
BpiI GAAGAC 2 cut(s) 240, 1177
BplI GAGNNNNNCTC 2 cut(s) 538, 570
BpuEI CTTGAG 2 cut(s) 263, 976
BsaAI YACGTR 1 cut(s) 859
BsaHI GRCGYC 2 cut(s) 402, 1195
BsaJI CCNNGG 4 cut(s) 603, 625, 1057, 1189
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 244
Bse1I ACTGG 2 cut(s) 321, 1280
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 2 cut(s) 812, 1059
BseDI CCNNGG 4 cut(s) 603, 625, 1057, 1189
BseGI GGATG 5 cut(s) 294, 445, 706, 1261, 1273
BseLI CCNNNNNNNGG 2 cut(s) 88, 244
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 2 cut(s) 321, 1280
Bsh1236I CGCG 2 cut(s) 220, 333
BshFI GGCC 2 cut(s) 93, 168
BshNI GGYRCC 2 cut(s) 401, 1128
BsiHKCI CYCGRG 1 cut(s) 602
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 88, 244
BsmAI GTCTC 2 cut(s) 366, 758
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 891
BsnI GGCC 2 cut(s) 93, 168
BsoBI CYCGRG 1 cut(s) 602
Bsp1407I TGTACA 2 cut(s) 63, 184
Bsp143I GATC 2 cut(s) 807, 1341
BspACI CCGC 4 cut(s) 333, 889, 932, 1036
BspANI GGCC 2 cut(s) 93, 168
BspCNI CTCAG 1 cut(s) 126
BspFNI CGCG 2 cut(s) 220, 333
BspLI GGNNCC 5 cut(s) 169, 337, 374, 403, 1130
BspPI GGATC 1 cut(s) 802
BspT107I GGYRCC 2 cut(s) 401, 1128
BsrDI GCAATG 1 cut(s) 486
BsrGI TGTACA 2 cut(s) 63, 184
BsrI ACTGG 2 cut(s) 321, 1280
BssECI CCNNGG 4 cut(s) 603, 625, 1057, 1189
BssMI GATC 2 cut(s) 807, 1341
BssNI GRCGYC 2 cut(s) 402, 1195
BssSI CACGAG 1 cut(s) 947
BssT1I CCWWGG 2 cut(s) 625, 1189
Bst2BI CACGAG 1 cut(s) 947
Bst2UI CCWGG 2 cut(s) 812, 1059
Bst4CI ACNGT 2 cut(s) 967, 1099
Bst6I CTCTTC 2 cut(s) 125, 468
BstACI GRCGYC 2 cut(s) 402, 1195
BstAPI GCANNNNNTGC 1 cut(s) 32
BstAUI TGTACA 2 cut(s) 63, 184
BstBAI YACGTR 1 cut(s) 859
BstC8I GCNNGC 2 cut(s) 50, 1218
BstDEI CTNAG 3 cut(s) 113, 229, 1106
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 5 cut(s) 294, 445, 706, 1261, 1273
BstFNI CGCG 2 cut(s) 220, 333
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 3 cut(s) 220, 333, 404
BstKTI GATC 2 cut(s) 810, 1344
BstMAI GTCTC 2 cut(s) 366, 758
BstMBI GATC 2 cut(s) 807, 1341
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstNI CCWGG 2 cut(s) 812, 1059
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 810, 1057
BstUI CGCG 2 cut(s) 220, 333
BstV2I GAAGAC 2 cut(s) 240, 1177
BstX2I RGATCY 1 cut(s) 807
BstYI RGATCY 1 cut(s) 807
BsuRI GGCC 2 cut(s) 93, 168
BtsCI GGATG 5 cut(s) 294, 445, 706, 1261, 1273
Cac8I GCNNGC 2 cut(s) 50, 1218
CfoI GCGC 3 cut(s) 220, 333, 404
Cfr13I GGNCC 3 cut(s) 91, 167, 335
CseI GACGC 1 cut(s) 1184
Csp6I GTAC 3 cut(s) 64, 185, 897
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviAII CATG 4 cut(s) 308, 316, 425, 566
CviQI GTAC 3 cut(s) 64, 185, 897
DdeI CTNAG 3 cut(s) 113, 229, 1106
DinI GGCGCC 1 cut(s) 403
DpnI GATC 2 cut(s) 809, 1343
DpnII GATC 2 cut(s) 807, 1341
DraI TTTAAA 2 cut(s) 442, 739
DrdI GACNNNNNNGTC 1 cut(s) 1089
DriI GACNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 1089
Eam1104I CTCTTC 2 cut(s) 125, 468
Eam1105I GACNNNNNGTC 1 cut(s) 316
EarI CTCTTC 2 cut(s) 125, 468
Eco130I CCWWGG 2 cut(s) 625, 1189
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 602
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 810, 1057
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 2 cut(s) 625, 1189
EgeI GGCGCC 1 cut(s) 403
EheI GGCGCC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 625, 1189
FaeI CATG 4 cut(s) 311, 319, 428, 569
FalI AAGNNNNNCTT 4 cut(s) 761, 793, 838, 870
FaqI GGGAC 1 cut(s) 260
FatI CATG 4 cut(s) 307, 315, 424, 565
FauI CCCGC 1 cut(s) 1029
FbaI TGATCA 1 cut(s) 1341
FokI GGATG 5 cut(s) 281, 432, 713, 1268, 1280
FspBI CTAG 4 cut(s) 257, 1190, 1206, 1323
GlaI GCGC 3 cut(s) 219, 332, 403
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 2 cut(s) 93, 168
HgaI GACGC 1 cut(s) 1184
HhaI GCGC 3 cut(s) 220, 333, 404
Hin1I GRCGYC 2 cut(s) 402, 1195
Hin1II CATG 4 cut(s) 311, 319, 428, 569
Hin6I GCGC 3 cut(s) 218, 331, 402
HinP1I GCGC 3 cut(s) 218, 331, 402
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 5 cut(s) 17, 248, 617, 1139, 1311
HphI GGTGA 1 cut(s) 1283
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 5 cut(s) 327, 450, 614, 1149, 1164
Hpy188III TCNNGA 7 cut(s) 40, 280, 363, 583, 947, 955, 1048
Hpy8I GTNNAC 1 cut(s) 412
Hpy99I CGWCG 1 cut(s) 1200
HpyAV CCTTC 4 cut(s) 46, 181, 724, 787
HpyCH4III ACNGT 2 cut(s) 967, 1099
HpyCH4IV ACGT 2 cut(s) 858, 1084
HpyCH4V TGCA 2 cut(s) 26, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 3 cut(s) 113, 229, 1106
HpySE526I ACGT 2 cut(s) 858, 1084
Hsp92I GRCGYC 2 cut(s) 402, 1195
Hsp92II CATG 4 cut(s) 311, 319, 428, 569
HspAI GCGC 3 cut(s) 218, 331, 402
KasI GGCGCC 1 cut(s) 401
Ksp22I TGATCA 1 cut(s) 1341
Kzo9I GATC 2 cut(s) 807, 1341
LmnI GCTCC 4 cut(s) 110, 309, 1039, 1225
MaeI CTAG 4 cut(s) 257, 1190, 1206, 1323
MaeII ACGT 2 cut(s) 858, 1084
MaeIII GTNAC 2 cut(s) 154, 1271
MalI GATC 2 cut(s) 809, 1343
MboI GATC 2 cut(s) 807, 1341
MboII GAAGA 7 cut(s) 112, 245, 485, 847, 935, 985, 1177
MflI RGATCY 1 cut(s) 807
MluCI AATT 9 cut(s) 175, 210, 590, 694, 711, 734, 787, 838, 1230
Mly113I GGCGCC 1 cut(s) 402
MlyI GAGTC 2 cut(s) 242, 1148
MmeI TCCRAC 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 510
MseI TTAA 6 cut(s) 387, 441, 464, 738, 1029, 1233
MspR9I CCNGG 2 cut(s) 812, 1059
MssI GTTTAAAC 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 891
MvaI CCWGG 2 cut(s) 812, 1059
MvnI CGCG 2 cut(s) 220, 333
MwoI GCNNNNNNNGC 1 cut(s) 32
NarI GGCGCC 1 cut(s) 402
NdeII GATC 2 cut(s) 807, 1341
NlaIII CATG 4 cut(s) 311, 319, 428, 569
NlaIV GGNNCC 5 cut(s) 169, 337, 374, 403, 1130
NmuCI GTSAC 2 cut(s) 154, 1271
NspI RCATGY 1 cut(s) 428
PciI ACATGT 1 cut(s) 424
PctI GAATGC 1 cut(s) 891
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 3 cut(s) 17, 617, 1311
PflFI GACNNNGTC 1 cut(s) 1181
PleI GAGTC 2 cut(s) 242, 1147
PluTI GGCGCC 1 cut(s) 405
PmeI GTTTAAAC 1 cut(s) 442
PpsI GAGTC 2 cut(s) 242, 1147
Ppu21I YACGTR 1 cut(s) 859
PscI ACATGT 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 810, 1057
PspGI CCWGG 2 cut(s) 810, 1057
PspN4I GGNNCC 5 cut(s) 169, 337, 374, 403, 1130
PspPI GGNCC 3 cut(s) 91, 167, 335
PsuI RGATCY 1 cut(s) 807
PsyI GACNNNGTC 1 cut(s) 1181
RsaI GTAC 3 cut(s) 65, 186, 898
RsaNI GTAC 3 cut(s) 64, 185, 897
SaqAI TTAA 6 cut(s) 387, 441, 464, 738, 1029, 1233
Sau3AI GATC 2 cut(s) 807, 1341
Sau96I GGNCC 3 cut(s) 91, 167, 335
SchI GAGTC 2 cut(s) 242, 1148
ScrFI CCNGG 2 cut(s) 812, 1059
SfoI GGCGCC 1 cut(s) 403
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 278, 955
SmoI CTYRAG 2 cut(s) 278, 955
SpeI ACTAGT 1 cut(s) 1322
Sse9I AATT 9 cut(s) 175, 210, 590, 694, 711, 734, 787, 838, 1230
SsiI CCGC 4 cut(s) 333, 889, 932, 1036
SspDI GGCGCC 1 cut(s) 401
SspI AATATT 2 cut(s) 635, 1065
SspMI CTAG 4 cut(s) 257, 1190, 1206, 1323
StyD4I CCNGG 2 cut(s) 810, 1057
StyI CCWWGG 2 cut(s) 625, 1189
TaaI ACNGT 2 cut(s) 967, 1099
TaiI ACGT 2 cut(s) 861, 1087
TaqI TCGA 6 cut(s) 123, 251, 288, 364, 504, 1198
TaqII GACCGA 1 cut(s) 252
TasI AATT 9 cut(s) 175, 210, 590, 694, 711, 734, 787, 838, 1230
TatI WGTACW 2 cut(s) 63, 184
TfiI GAWTC 3 cut(s) 17, 617, 1311
Tru1I TTAA 6 cut(s) 387, 441, 464, 738, 1029, 1233
Tru9I TTAA 6 cut(s) 387, 441, 464, 738, 1029, 1233
TseFI GTSAC 2 cut(s) 154, 1271
Tsp45I GTSAC 2 cut(s) 154, 1271
TspDTI ATGAA 3 cut(s) 20, 503, 503
Tth111I GACNNNGTC 1 cut(s) 1181
VpaK11BI GGWCC 1 cut(s) 335
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 4 cut(s) 590, 694, 734, 787
XceI RCATGY 1 cut(s) 428
XmaJI CCTAGG 1 cut(s) 1189
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 4 cut(s) 257, 1190, 1206, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.