MD07G1081900.v1.1

APO protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0017486
Physical Location & Seq
Reverse (-)
8014939 .. 8018932
3994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1081900.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGAAGGTTTTGATTCGAAGAGCCAAGGAGGAGAAAGAAGCCCGGAAGGCTGAGCCTTGCAGGATGCTTGAAGAATCACCTGATAATGGATTGTTGGTCCCTGAATTGGTTGAGGTAGCTCATCAAGTTTACCAAGCTCGAAAGTCCCTCCTGTTTGGCTTGTCAAAACTTGTTCAGGTTGTTCCTGTCCAACGGTGCAGCTTTTGCTCTGAAGTTCACATTGGACATGTCGGCAGTTTTAGGAGTGGGACACAAGTGTGGAGGAAGGGAGGAGTTCAACATGTGGTTTCCTTTCCTAAATGCTTCCATCTTTATGACCGTGTTGGAAAACCAAGAGTTGGGCATGATGAGAGGTTTGAAATCCCACGAATCCCTGCCATTTTGGAGCTCTGTATACAGGCTGGTTTAGAGATTGACAAGTACCCAACAAAGAGAAGAACGAGACCTGTATATAGTATCGAAGGAAGAATTGTAGATTTTGAGTCGATTAACAAAATGGATGATGTGAATAGGAACTCATATTCTGGGATTGTTGATCTCGTAGTACATTCTGATATTAAAACTGATGTTGAGGAGTTGAGGAATTCAGATTCACAGAGTGATACTAACCTTGTGGATAAAACATTACCGAGCGATAAAGTGGGGAAGTCTTTGAATGAGTTAAGTATTATTACAATGGACTCATGGTTTGAGATGATATCCGGAGCAAAGAAGATCATGGAGAAATACAGTGTGAAGACTTGTGGATATTGTTTAGAGGTTCAGGTAGGTCCCAAGGGGCACAAAGTGAGGATGTGCAAGGCTTCAAAGCATCAATCTCGTAATGGTTTGCATGCTTGGCAAGAAGCAACAATAGACGATCTTGTGGGTCCGAATTATGTTTGGCATGTCCGCGACATAGATGGTCCTGCTCTGGATAACAACCTGAACAGGTATTACGGAAAGGCTCCAGCTGTGGTGGAGCTATGCGTGCAGGCAGGCGCACCTGTGCCAAATGAATATAGGAGTATGATAAGATTAGATGTAGCTTCTCCAGAGCGCAACGAGGTTGATCTGGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

355

Amino Acids

39.98

Weight (kDa)

8.01

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 1 - 159 2.4e-55 APO RNA-binding
APO_RNA-bind PF05634 202 - 335 2.6e-25 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 338
AccI GTMKAC 1 cut(s) 394
AccII CGCG 1 cut(s) 894
AccIII TCCGGA 1 cut(s) 701
AciI CCGC 1 cut(s) 892
AcsI RAATTY 1 cut(s) 583
AcuI CTGAAG 1 cut(s) 231
AdeI CACNNNGTG 2 cut(s) 599, 787
AfaI GTAC 2 cut(s) 422, 546
AfiI CCNNNNNNNGG 3 cut(s) 86, 106, 338
AflIII ACRYGT 2 cut(s) 226, 280
AgsI TTSAA 5 cut(s) 71, 278, 359, 655, 807
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AleI CACNNNNGTG 1 cut(s) 256
AluBI AGCT 7 cut(s) 119, 137, 201, 388, 953, 964, 1028
AluI AGCT 7 cut(s) 119, 137, 201, 388, 953, 964, 1028
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 436
Aor13HI TCCGGA 1 cut(s) 701
ApeKI GCWGC 1 cut(s) 198
ApoI RAATTY 1 cut(s) 583
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AspLEI GCGC 2 cut(s) 983, 1041
AspS9I GGNCC 4 cut(s) 97, 770, 869, 905
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 69
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 4 cut(s) 97, 770, 869, 905
BaeGI GKGCMC 1 cut(s) 783
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 743
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 210
BccI CCATC 2 cut(s) 315, 896
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 436
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 1 cut(s) 199
BlpI GCTNAGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 200
Bme1390I CCNGG 1 cut(s) 43
Bme18I GGWCC 4 cut(s) 97, 770, 869, 905
BmgT120I GGNCC 4 cut(s) 97, 770, 869, 905
BmiI GGNNCC 4 cut(s) 99, 772, 870, 948
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 2 cut(s) 54, 820
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 2 cut(s) 933, 1017
Bpu1102I GCTNAGC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 43
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 24, 774
BsaWI WCCGGW 1 cut(s) 701
Bsc4I CCNNNNNNNGG 3 cut(s) 86, 106, 338
BseAI TCCGGA 1 cut(s) 701
BseDI CCNNGG 2 cut(s) 24, 774
BseGI GGATG 3 cut(s) 69, 505, 798
BseLI CCNNNNNNNGG 3 cut(s) 86, 106, 338
BseMII CTCAG 1 cut(s) 42
BseRI GAGGAG 3 cut(s) 44, 285, 587
BseSI GKGCMC 1 cut(s) 783
BseXI GCAGC 1 cut(s) 210
BsgI GTGCAG 2 cut(s) 217, 992
Bsh1236I CGCG 1 cut(s) 894
BsiHKAI GWGCWC 1 cut(s) 390
BsiSI CCGG 2 cut(s) 43, 702
BslFI GGGAC 4 cut(s) 83, 130, 262, 756
BslI CCNNNNNNNGG 3 cut(s) 86, 106, 338
BsmAI GTCTC 1 cut(s) 436
BsmFI GGGAC 4 cut(s) 83, 130, 262, 756
Bso31I GGTCTC 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 390, 783
Bsp13I TCCGGA 1 cut(s) 701
Bsp143I GATC 4 cut(s) 535, 714, 859, 1051
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 1 cut(s) 892
BspCNI CTCAG 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 701
BspFNI CGCG 1 cut(s) 894
BspLI GGNNCC 4 cut(s) 99, 772, 870, 948
BspQI GCTCTTC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 436
BssECI CCNNGG 2 cut(s) 24, 774
BssMI GATC 4 cut(s) 535, 714, 859, 1051
BssNAI GTATAC 1 cut(s) 395
BssT1I CCWWGG 2 cut(s) 24, 774
Bst1107I GTATAC 1 cut(s) 395
Bst4CI ACNGT 3 cut(s) 195, 320, 731
Bst6I CTCTTC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 4 cut(s) 834, 971, 975, 979
BstDEI CTNAG 1 cut(s) 51
BstF5I GGATG 3 cut(s) 69, 505, 798
BstFNI CGCG 1 cut(s) 894
BstHHI GCGC 2 cut(s) 983, 1041
BstKTI GATC 4 cut(s) 538, 717, 862, 1054
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 4 cut(s) 535, 714, 859, 1051
BstMWI GCNNNNNNNGC 4 cut(s) 47, 204, 838, 970
BstNSI RCATGY 4 cut(s) 230, 284, 836, 890
BstSCI CCNGG 1 cut(s) 41
BstSLI GKGCMC 1 cut(s) 783
BstUI CGCG 1 cut(s) 894
BstV1I GCAGC 1 cut(s) 210
BstV2I GAAGAC 1 cut(s) 743
BstZ17I GTATAC 1 cut(s) 395
BtsCI GGATG 3 cut(s) 69, 505, 798
BtsIMutI CAGTG 1 cut(s) 736
Cac8I GCNNGC 4 cut(s) 834, 971, 975, 979
CfoI GCGC 2 cut(s) 983, 1041
Cfr13I GGNCC 4 cut(s) 97, 770, 869, 905
Csp6I GTAC 2 cut(s) 421, 545
CviAII CATG 7 cut(s) 227, 281, 344, 684, 718, 833, 887
CviQI GTAC 2 cut(s) 421, 545
DdeI CTNAG 1 cut(s) 51
DpnI GATC 4 cut(s) 537, 716, 861, 1053
DpnII GATC 4 cut(s) 535, 714, 859, 1051
DraIII CACNNNGTG 2 cut(s) 599, 787
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 388
Eco130I CCWWGG 2 cut(s) 24, 774
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 436
Eco32I GATATC 1 cut(s) 699
Eco47I GGWCC 4 cut(s) 97, 770, 869, 905
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 231
EcoICRI GAGCTC 1 cut(s) 388
EcoO109I RGGNCCY 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 583
EcoRV GATATC 1 cut(s) 699
EcoT14I CCWWGG 2 cut(s) 24, 774
EcoT38I GRGCYC 1 cut(s) 390
ErhI CCWWGG 2 cut(s) 24, 774
FaeI CATG 7 cut(s) 230, 284, 347, 687, 721, 836, 890
FaqI GGGAC 4 cut(s) 83, 130, 262, 756
FatI CATG 7 cut(s) 226, 280, 343, 683, 717, 832, 886
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 199
FokI GGATG 3 cut(s) 76, 512, 805
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 1 cut(s) 199
GlaI GCGC 2 cut(s) 982, 1040
GluI GCNGC 1 cut(s) 199
GsuI CTGGAG 2 cut(s) 933, 1017
HapII CCGG 2 cut(s) 43, 702
HhaI GCGC 2 cut(s) 983, 1041
Hin1II CATG 7 cut(s) 230, 284, 347, 687, 721, 836, 890
Hin6I GCGC 2 cut(s) 981, 1039
HinP1I GCGC 2 cut(s) 981, 1039
HinfI GANTC 6 cut(s) 13, 74, 369, 482, 590, 680
HpaII CCGG 2 cut(s) 43, 702
HphI GGTGA 1 cut(s) 69
Hpy166II GTNNAC 3 cut(s) 130, 217, 395
Hpy188I TCNGA 4 cut(s) 211, 553, 589, 873
Hpy188III TCNNGA 3 cut(s) 702, 914, 1034
Hpy8I GTNNAC 3 cut(s) 130, 217, 395
HpyAV CCTTC 3 cut(s) 40, 259, 455
HpyCH4III ACNGT 3 cut(s) 195, 320, 731
HpyCH4V TGCA 5 cut(s) 60, 198, 798, 832, 973
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 204, 838, 970
HpyF3I CTNAG 1 cut(s) 51
Hsp92II CATG 7 cut(s) 230, 284, 347, 687, 721, 836, 890
HspAI GCGC 2 cut(s) 981, 1039
Kpn2I TCCGGA 1 cut(s) 701
Kzo9I GATC 4 cut(s) 535, 714, 859, 1051
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 4 cut(s) 385, 704, 952, 961
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 2 cut(s) 54, 820
MalI GATC 4 cut(s) 537, 716, 861, 1053
MboI GATC 4 cut(s) 535, 714, 859, 1051
MboII GAAGA 6 cut(s) 30, 83, 447, 477, 724, 748
MhlI GDGCHC 2 cut(s) 390, 783
MluCI AATT 4 cut(s) 104, 468, 583, 874
MlyI GAGTC 2 cut(s) 491, 674
MmeI TCCRAC 2 cut(s) 214, 304
MroI TCCGGA 1 cut(s) 701
MseI TTAA 3 cut(s) 489, 558, 662
MslI CAYNNNNRTG 2 cut(s) 256, 312
MspA1I CMGCKG 1 cut(s) 953
MspI CCGG 2 cut(s) 43, 702
MspR9I CCNGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 894
MwoI GCNNNNNNNGC 4 cut(s) 47, 204, 838, 970
NciI CCSGG 1 cut(s) 43
NdeII GATC 4 cut(s) 535, 714, 859, 1051
NlaIII CATG 7 cut(s) 230, 284, 347, 687, 721, 836, 890
NlaIV GGNNCC 4 cut(s) 99, 772, 870, 948
NspI RCATGY 4 cut(s) 230, 284, 836, 890
NspV TTCGAA 1 cut(s) 16
OliI CACNNNNGTG 1 cut(s) 256
PaeI GCATGC 1 cut(s) 836
PciI ACATGT 2 cut(s) 226, 280
PciSI GCTCTTC 1 cut(s) 13
PfeI GAWTC 4 cut(s) 13, 74, 369, 590
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 1 cut(s) 200
PleI GAGTC 2 cut(s) 490, 674
PpsI GAGTC 2 cut(s) 490, 674
PpuMI RGGWCCY 1 cut(s) 770
PscI ACATGT 2 cut(s) 226, 280
Psp124BI GAGCTC 1 cut(s) 390
Psp5II RGGWCCY 1 cut(s) 770
PspN4I GGNNCC 4 cut(s) 99, 772, 870, 948
PspPI GGNCC 4 cut(s) 97, 770, 869, 905
PspPPI RGGWCCY 1 cut(s) 770
PsrI GAACNNNNNNTAC 2 cut(s) 920, 952
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 2 cut(s) 422, 546
RsaNI GTAC 2 cut(s) 421, 545
RseI CAYNNNNRTG 2 cut(s) 256, 312
SacI GAGCTC 1 cut(s) 390
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 3 cut(s) 489, 558, 662
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 4 cut(s) 535, 714, 859, 1051
Sau96I GGNCC 4 cut(s) 97, 770, 869, 905
SchI GAGTC 2 cut(s) 491, 674
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 2 cut(s) 390, 783
SfaNI GCATC 2 cut(s) 54, 820
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 4 cut(s) 97, 770, 869, 905
SmiMI CAYNNNNRTG 2 cut(s) 256, 312
SphI GCATGC 1 cut(s) 836
Sse9I AATT 4 cut(s) 104, 468, 583, 874
SsiI CCGC 1 cut(s) 892
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 1 cut(s) 41
StyI CCWWGG 2 cut(s) 24, 774
TaaI ACNGT 3 cut(s) 195, 320, 731
TaqI TCGA 4 cut(s) 16, 139, 459, 485
TasI AATT 4 cut(s) 104, 468, 583, 874
TatI WGTACW 1 cut(s) 544
TfiI GAWTC 4 cut(s) 13, 74, 369, 590
Tru1I TTAA 3 cut(s) 489, 558, 662
Tru9I TTAA 3 cut(s) 489, 558, 662
TscAI CASTG 1 cut(s) 736
TseI GCWGC 1 cut(s) 198
TspDTI ATGAA 2 cut(s) 17, 1011
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 1 cut(s) 736
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 4 cut(s) 97, 770, 869, 905
XapI RAATTY 1 cut(s) 583
XceI RCATGY 4 cut(s) 230, 284, 836, 890
XmiI GTMKAC 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.