Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010706

Physical Location & Seq

Genomic Coordinates Forward (+)

21861687 .. 21864623

Gene Structure 2937 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1150000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGTCGTCGGCCGTTATTGCTGCTCTTACTCCGGTTCACAGTGAGCCCGCGTTTCCCCAATCGTGTATGTCTTCGGATGAATCGATATTGATATATCTCTCTGTGTCTGGGTCAACTACTCCTCTGAGTGTTTTGGGATCGGACTCTATTGAATCAGTGAAGCTTAGGATACAAACCTATAAAGGGTTTGTTGCTAAGAAGCAAAAGTTAGTTTGTGGAGGTAGAGAGCTGGCTAGGAGCAATTCCATACTCCGGGAATACGGGGTCACTGATGGGAATATTCTACATTTGGTCCTTAGGCTTTCTGACCTTCAAGAGATCAATGTCCGAACGACTTGCGGTAAAGAGTTTACCTTCCACGTGGAACGTGGTCAAGATGTTGGATATGTGAAGCAAAAGATTGCAAAGAAGAGTAAAGAGTTTGTTGATCTAGAAGAACAAGAAGTTGTGTATGATGGAAAACTACTTGAAGATCAGAGACTTATTGATGACATATGTAAACATAATGATGCTGTCTTACATCTGTTGGTCCGGAAGTCTGCAAAAGTTAGGGCCAGGCCAGTTGTGAAGAACTTTGAATTGTCAGTTGTGGCGTCACAGTTGGATGATGGGAAAAATTATGAAGTTGGTGGAGATAATGTGACGCGACAATATGATGAGCATGACTTTAAGAGAGATTATGAAGTTGTGCTGAGAAAACCTCCTGACAGGGGATTTTTGTTGGAGCCTGTCATTGTTAACCGCAAGATTGAATTACCTTCCCAGATTCGGGATATGGTTCATGACACGTCTGTCGGACTAGATAGTGGGAGTTATCCAATCAGGTCCATGGAGGGTACAGGAGGAGCGTACTTTATGCTAGACTCGTCAGGTCAAAAGTATGTATCTGTTTTTAAGCCGATTGATGAGGAGCCTATGGCTCAAAACAATCCGAGGGGGCTGCCATTGTCATTGGATGGTGAAGGATTGAAAAAGGGAACAAGAGTTGGAGAAGGAGCATTTAGGGAAGTGGCAGCTTACCTCTTGGATCATCCAATGGGTGGATATCGCATGCCCTTTGGCAATGCAAAGGGCTTTGCTGGGGTCCCCCCTACATTTATTGTCAAGTGCTTCCATAAAGTGTTCAACCATCCTGGGGATGCCACTGTTAAGATCGGGTCCTTGCAGAAGTTCATGGAGAATAGTGGAAGTTGCGAAGACATGGGCCCTGCTGCTTTCCCAACTGAGGAAGTGCATAAGATTTCTGTTTTAGATATCAGATTGGCAAATGCGGACAGGCATGCTGGAAACATTTTGCTGGGCAAAGAGGGTGAAGATGGCCAGACTGTGCTGATTCCAATTGATCATGGCTACTGCTTACCTGAAACATTTGAAGACATTACATTTGACTGGTTGTATTGGCCACAAGCACGCAAGCCTTATGCTGACGAGGTCATCGATTACATCAAATCACTGGATGCAGAAGAAGATATTGCCATTTTGAAGTTCCATGGATGGGACATGCCCCGAAAATGTGCCCGCACACTTCGCATTTCAACCATGCTGTTGAAGAAAGGGGCAGAGAGACAGTTCACTCCCTTTGCTATAGGAAACATAATGTGCAGAAAAACCCTGAACAAGGAATCCATCATTGAGGAGATGGTGCAAGAAGCAGAGGATTCTGTGCTCCCGGATTCAAGTGAAGATTCTTTTCTTGAGACCGTGTCCCAAATTATGGACCGTCGCCTTGAAGAGATTGCAAGATCACTTTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PI3_PI4_kinase ubiquitin

Protein Analysis

585

Amino Acids

65.25

Weight (kDa)

5.48

Isoelectric Point (pI)

45.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ubiquitin	PF00240	33 - 101	3.5e-12	Ubiquitin family
ubiquitin	PF00240	107 - 179	4.2e-12	Ubiquitin family
PI3_PI4_kinase	PF00454	280 - 527	3.4e-47	Phosphatidylinositol 3- and 4-kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010706)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46500 AT2G46500 AT2G46500
fragaria_vesca	FvH4_7g16460 FvH4_7g16460
malus_domestica	MD01G1081200.v1.1 MD07G1150000.v1.1
prunus_persica	Prupe.2G188900_v2.0.a1
pyrus_communis	pycom07g14860
rosa_chinensis	RchiOBHm_Chr1g0359561
rosa_laevigata	RLG00000027862
rosa_multiflora	Rmu_co8470243.1_g000001 Rmu_sc0002556.1_g000019 Rmu_sc0013097.1_g000002
rosa_rugosa	Rorug01G0272200
rosa_samantha	Rh1AG285400 Rh1BG251600 Rh1CG269200 Rh1DG280600
rosa_wichuraiana	Rw1G025380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	791
AccB7I	CCANNNNNTGG	2 cut(s)	1040, 1714
AccII	CGCG	2 cut(s)	50, 646
AccIII	TCCGGA	1 cut(s)	531
AciI	CCGC	5 cut(s)	48, 339, 742, 1271, 1519
AclWI	GGATC	2 cut(s)	145, 1035
AcoI	YGGCCR	3 cut(s)	9, 1318, 1400
AcvI	CACGTG	1 cut(s)	361
AcyI	GRCGYC	1 cut(s)	593
AfaI	GTAC	2 cut(s)	838, 851
AflIII	ACRYGT	1 cut(s)	786
AjiI	CACGTC	1 cut(s)	789
AjnI	CCWGG	2 cut(s)	554, 1132
AloI	GAACNNNNNNTCC	2 cut(s)	1607, 1639
AluBI	AGCT	3 cut(s)	163, 229, 1016
AluI	AGCT	3 cut(s)	163, 229, 1016
Alw21I	GWGCWC	1 cut(s)	1668
Alw26I	GTCTC	3 cut(s)	472, 1558, 1691
AlwI	GGATC	2 cut(s)	145, 1035
Aor13HI	TCCGGA	1 cut(s)	531
AoxI	GGCC	6 cut(s)	9, 552, 557, 1204, 1318, 1400
ApaI	GGGCCC	1 cut(s)	1208
ApeKI	GCWGC	4 cut(s)	20, 940, 1013, 1211
ArsI	GACNNNNNNTTYG	2 cut(s)	1367, 1399
AspS9I	GGNCC	9 cut(s)	292, 529, 552, 825, 1084, 1158, 1204, 1205, 1717
AsuC2I	CCSGG	2 cut(s)	254, 1670
AsuHPI	GGTGA	2 cut(s)	971, 1322
AvaII	GGWCC	6 cut(s)	292, 529, 825, 1084, 1158, 1717
AxyI	CCTNAGG	1 cut(s)	296
BaeGI	GKGCMC	2 cut(s)	1208, 1519
BalI	TGGCCA	2 cut(s)	1320, 1402
BanII	GRGCYC	2 cut(s)	48, 1208
BbrPI	CACGTG	1 cut(s)	361
BbsI	GAAGAC	3 cut(s)	63, 1203, 1380
Bbv12I	GWGCWC	1 cut(s)	1668
BbvI	GCAGC	4 cut(s)	7, 927, 1025, 1198
BccI	CCATC	9 cut(s)	266, 449, 602, 950, 1137, 1310, 1488, 1633, 1634
BcgI	CGANNNNNNTGC	4 cut(s)	318, 352, 922, 956
BciT130I	CCWGG	2 cut(s)	556, 1134
BciVI	GTATCC	1 cut(s)	162
BclI	TGATCA	1 cut(s)	1342
BcnI	CCSGG	2 cut(s)	254, 1670
BcoDI	GTCTC	3 cut(s)	472, 1558, 1691
BfaI	CTAG	4 cut(s)	234, 431, 800, 860
BfmI	CTRYAG	1 cut(s)	1584
BfuI	GTATCC	1 cut(s)	162
BisI	GCNGC	4 cut(s)	21, 941, 1014, 1212
BlsI	GCNGC	4 cut(s)	22, 942, 1015, 1213
Bme1390I	CCNGG	4 cut(s)	254, 556, 1134, 1670
Bme18I	GGWCC	6 cut(s)	292, 529, 825, 1084, 1158, 1717
BmgBI	CACGTC	1 cut(s)	789
BmgT120I	GGNCC	9 cut(s)	292, 529, 552, 825, 1084, 1158, 1204, 1205, 1717
BmiI	GGNNCC	6 cut(s)	726, 912, 1085, 1086, 1159, 1206
BmrFI	CCNGG	4 cut(s)	254, 556, 1134, 1670
BmsI	GCATC	3 cut(s)	499, 1129, 1447
BpiI	GAAGAC	3 cut(s)	63, 1203, 1380
BplI	GAGNNNNNCTC	2 cut(s)	685, 717
Bpu10I	CCTNAGC	1 cut(s)	164
BpuEI	CTTGAG	1 cut(s)	1715
BpuMI	CCSGG	2 cut(s)	254, 1670
Bsa29I	ATCGAT	2 cut(s)	83, 1437
BsaAI	YACGTR	1 cut(s)	361
BsaHI	GRCGYC	1 cut(s)	593
BsaI	GGTCTC	1 cut(s)	1691
BsaJI	CCNNGG	4 cut(s)	828, 932, 1133, 1489
BsaWI	WCCGGW	2 cut(s)	31, 531
BsaXI	ACNNNNNCTCC	2 cut(s)	624, 654
Bse1I	ACTGG	3 cut(s)	560, 1394, 1458
Bse21I	CCTNAGG	1 cut(s)	296
Bse3DI	GCAATG	1 cut(s)	1069
BseAI	TCCGGA	1 cut(s)	531
BseBI	CCWGG	2 cut(s)	556, 1134
BseCI	ATCGAT	2 cut(s)	83, 1437
BseDI	CCNNGG	4 cut(s)	828, 932, 1133, 1489
BseGI	GGATG	8 cut(s)	82, 610, 961, 1030, 1129, 1144, 1462, 1499
BseMI	GCAATG	1 cut(s)	1069
BseMII	CTCAG	3 cut(s)	116, 683, 1215
BseNI	ACTGG	3 cut(s)	560, 1394, 1458
BseRI	GAGGAG	4 cut(s)	111, 858, 923, 1649
BseSI	GKGCMC	2 cut(s)	1208, 1519
BseX3I	CGGCCG	1 cut(s)	9
BseXI	GCAGC	4 cut(s)	7, 927, 1025, 1198
BseYI	CCCAGC	2 cut(s)	1079, 1297
BsgI	GTGCAG	1 cut(s)	1621
Bsh1236I	CGCG	2 cut(s)	50, 646
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	6 cut(s)	11, 554, 559, 1206, 1320, 1402
BshVI	ATCGAT	2 cut(s)	83, 1437
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	1 cut(s)	1668
BsiSI	CCGG	4 cut(s)	32, 253, 532, 1670
BslFI	GGGAC	3 cut(s)	1070, 1511, 1690
BsmAI	GTCTC	3 cut(s)	472, 1558, 1691
BsmFI	GGGAC	3 cut(s)	1070, 1511, 1690
BsnI	GGCC	6 cut(s)	11, 554, 559, 1206, 1320, 1402
Bso31I	GGTCTC	1 cut(s)	1691
Bsp120I	GGGCCC	1 cut(s)	1204
Bsp1286I	GDGCHC	4 cut(s)	48, 1208, 1519, 1668
Bsp13I	TCCGGA	1 cut(s)	531
Bsp143I	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
Bsp19I	CCATGG	2 cut(s)	828, 1489
BspACI	CCGC	5 cut(s)	48, 339, 742, 1271, 1519
BspANI	GGCC	6 cut(s)	11, 554, 559, 1206, 1320, 1402
BspCNI	CTCAG	3 cut(s)	117, 684, 1216
BspDI	ATCGAT	2 cut(s)	83, 1437
BspEI	TCCGGA	1 cut(s)	531
BspFNI	CGCG	2 cut(s)	50, 646
BspHI	TCATGA	1 cut(s)	781
BspLI	GGNNCC	6 cut(s)	726, 912, 1085, 1086, 1159, 1206
BspPI	GGATC	2 cut(s)	145, 1035
BspTNI	GGTCTC	1 cut(s)	1691
BsrDI	GCAATG	1 cut(s)	1069
BsrI	ACTGG	3 cut(s)	560, 1394, 1458
BssECI	CCNNGG	4 cut(s)	828, 932, 1133, 1489
BssMI	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
BssNI	GRCGYC	1 cut(s)	593
BssT1I	CCWWGG	2 cut(s)	828, 1489
Bst2UI	CCWGG	2 cut(s)	556, 1134
Bst4CI	ACNGT	7 cut(s)	41, 600, 1147, 1327, 1569, 1702, 1721
Bst6I	CTCTTC	2 cut(s)	404, 1725
BstACI	GRCGYC	1 cut(s)	593
BstBAI	YACGTR	1 cut(s)	361
BstC8I	GCNNGC	7 cut(s)	48, 231, 1052, 1281, 1411, 1415, 1519
BstDEI	CTNAG	6 cut(s)	125, 164, 195, 296, 692, 1224
BstDSI	CCRYGG	2 cut(s)	828, 1489
BstENI	CCTNNNNNAGG	1 cut(s)	1616
BstF5I	GGATG	8 cut(s)	82, 610, 961, 1030, 1129, 1144, 1462, 1499
BstFNI	CGCG	2 cut(s)	50, 646
BstKTI	GATC	8 cut(s)	140, 321, 430, 475, 1030, 1155, 1345, 1745
BstMAI	GTCTC	3 cut(s)	472, 1558, 1691
BstMBI	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
BstMCI	CGRYCG	1 cut(s)	12
BstMWI	GCNNNNNNNGC	2 cut(s)	17, 1527
BstNI	CCWGG	2 cut(s)	556, 1134
BstNSI	RCATGY	3 cut(s)	1054, 1283, 1504
BstSCI	CCNGG	4 cut(s)	252, 554, 1132, 1668
BstSFI	CTRYAG	1 cut(s)	1584
BstSLI	GKGCMC	2 cut(s)	1208, 1519
BstUI	CGCG	2 cut(s)	50, 646
BstV1I	GCAGC	4 cut(s)	7, 927, 1025, 1198
BstV2I	GAAGAC	3 cut(s)	63, 1203, 1380
BstZI	CGGCCG	1 cut(s)	9
Bsu15I	ATCGAT	2 cut(s)	83, 1437
Bsu36I	CCTNAGG	1 cut(s)	296
BsuI	GTATCC	1 cut(s)	162
BsuRI	GGCC	6 cut(s)	11, 554, 559, 1206, 1320, 1402
BsuTUI	ATCGAT	2 cut(s)	83, 1437
BtgI	CCRYGG	2 cut(s)	828, 1489
BtrI	CACGTC	1 cut(s)	789
BtsCI	GGATG	8 cut(s)	82, 610, 961, 1030, 1129, 1144, 1462, 1499
BtsIMutI	CAGTG	5 cut(s)	46, 162, 267, 1143, 1451
Cac8I	GCNNGC	7 cut(s)	48, 231, 1052, 1281, 1411, 1415, 1519
CciI	TCATGA	1 cut(s)	781
Cfr13I	GGNCC	9 cut(s)	292, 529, 552, 825, 1084, 1158, 1204, 1205, 1717
ClaI	ATCGAT	2 cut(s)	83, 1437
CseI	GACGC	2 cut(s)	582, 652
Csp6I	GTAC	2 cut(s)	837, 850
CviQI	GTAC	2 cut(s)	837, 850
DdeI	CTNAG	6 cut(s)	125, 164, 195, 296, 692, 1224
DpnI	GATC	8 cut(s)	139, 320, 429, 474, 1029, 1154, 1344, 1744
DpnII	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
DrdI	GACNNNNNNGTC	1 cut(s)	791
DseDI	GACNNNNNNGTC	1 cut(s)	791
EaeI	YGGCCR	3 cut(s)	9, 1318, 1400
EagI	CGGCCG	1 cut(s)	9
Eam1104I	CTCTTC	2 cut(s)	404, 1725
EarI	CTCTTC	2 cut(s)	404, 1725
EclXI	CGGCCG	1 cut(s)	9
Eco130I	CCWWGG	2 cut(s)	828, 1489
Eco24I	GRGCYC	2 cut(s)	48, 1208
Eco31I	GGTCTC	1 cut(s)	1691
Eco32I	GATATC	2 cut(s)	1046, 1255
Eco47I	GGWCC	6 cut(s)	292, 529, 825, 1084, 1158, 1717
Eco52I	CGGCCG	1 cut(s)	9
Eco72I	CACGTG	1 cut(s)	361
Eco81I	CCTNAGG	1 cut(s)	296
EcoNI	CCTNNNNNAGG	1 cut(s)	1616
EcoO109I	RGGNCCY	3 cut(s)	1084, 1158, 1205
EcoRII	CCWGG	2 cut(s)	554, 1132
EcoRV	GATATC	2 cut(s)	1046, 1255
EcoT14I	CCWWGG	2 cut(s)	828, 1489
EcoT38I	GRGCYC	2 cut(s)	48, 1208
ErhI	CCWWGG	2 cut(s)	828, 1489
FaqI	GGGAC	3 cut(s)	1070, 1511, 1690
FauI	CCCGC	2 cut(s)	55, 1526
FauNDI	CATATG	1 cut(s)	494
FbaI	TGATCA	1 cut(s)	1342
Fnu4HI	GCNGC	4 cut(s)	21, 941, 1014, 1212
FokI	GGATG	8 cut(s)	89, 617, 968, 1017, 1116, 1151, 1469, 1506
FriOI	GRGCYC	2 cut(s)	48, 1208
Fsp4HI	GCNGC	4 cut(s)	21, 941, 1014, 1212
FspBI	CTAG	4 cut(s)	234, 431, 800, 860
GluI	GCNGC	4 cut(s)	21, 941, 1014, 1212
GsaI	CCCAGC	2 cut(s)	1083, 1301
HaeIII	GGCC	6 cut(s)	11, 554, 559, 1206, 1320, 1402
HapII	CCGG	4 cut(s)	32, 253, 532, 1670
HgaI	GACGC	2 cut(s)	582, 652
Hin1I	GRCGYC	1 cut(s)	593
HincII	GTYRAC	2 cut(s)	114, 739
HindII	GTYRAC	2 cut(s)	114, 739
HindIII	AAGCTT	1 cut(s)	161
HpaI	GTTAAC	1 cut(s)	739
HpaII	CCGG	4 cut(s)	32, 253, 532, 1670
HphI	GGTGA	2 cut(s)	971, 1322
Hpy166II	GTNNAC	6 cut(s)	37, 114, 351, 500, 739, 1572
Hpy188I	TCNGA	9 cut(s)	76, 126, 142, 307, 329, 477, 797, 933, 1259
Hpy188III	TCNNGA	8 cut(s)	314, 374, 431, 532, 704, 770, 782, 1694
Hpy8I	GTNNAC	6 cut(s)	37, 114, 351, 500, 739, 1572
Hpy99I	CGWCG	2 cut(s)	10, 1725
HpyAV	CCTTC	5 cut(s)	320, 364, 768, 956, 986
HpyCH4III	ACNGT	7 cut(s)	41, 600, 1147, 1327, 1569, 1702, 1721
HpyCH4IV	ACGT	3 cut(s)	360, 367, 788
HpyCH4V	TGCA	9 cut(s)	404, 542, 1067, 1165, 1234, 1460, 1602, 1645, 1739
HpyF10VI	GCNNNNNNNGC	2 cut(s)	17, 1527
HpyF3I	CTNAG	6 cut(s)	125, 164, 195, 296, 692, 1224
HpySE526I	ACGT	3 cut(s)	360, 367, 788
Hsp92I	GRCGYC	1 cut(s)	593
KflI	GGGWCCC	1 cut(s)	1084
Kpn2I	TCCGGA	1 cut(s)	531
Ksp22I	TGATCA	1 cut(s)	1342
KspAI	GTTAAC	1 cut(s)	739
Kzo9I	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
LmnI	GCTCC	6 cut(s)	237, 724, 845, 910, 995, 1671
Lsp1109I	GCAGC	4 cut(s)	7, 927, 1025, 1198
LweI	GCATC	3 cut(s)	499, 1129, 1447
MaeI	CTAG	4 cut(s)	234, 431, 800, 860
MaeII	ACGT	3 cut(s)	360, 367, 788
MaeIII	GTNAC	3 cut(s)	265, 594, 640
MalI	GATC	8 cut(s)	139, 320, 429, 474, 1029, 1154, 1344, 1744
MboI	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
MfeI	CAATTG	1 cut(s)	1338
MhlI	GDGCHC	4 cut(s)	48, 1208, 1519, 1668
MlsI	TGGCCA	2 cut(s)	1320, 1402
MluCI	AATT	6 cut(s)	241, 578, 616, 752, 1338, 1710
MluNI	TGGCCA	2 cut(s)	1320, 1402
MlyI	GAGTC	2 cut(s)	137, 857
MmeI	TCCRAC	5 cut(s)	361, 582, 702, 775, 967
Mox20I	TGGCCA	2 cut(s)	1320, 1402
MroI	TCCGGA	1 cut(s)	531
MscI	TGGCCA	2 cut(s)	1320, 1402
MseI	TTAA	4 cut(s)	669, 738, 894, 1149
MslI	CAYNNNNRTG	1 cut(s)	507
Msp20I	TGGCCA	2 cut(s)	1320, 1402
MspI	CCGG	4 cut(s)	32, 253, 532, 1670
MspR9I	CCNGG	4 cut(s)	254, 556, 1134, 1670
MunI	CAATTG	1 cut(s)	1338
MvaI	CCWGG	2 cut(s)	556, 1134
MvnI	CGCG	2 cut(s)	50, 646
MwoI	GCNNNNNNNGC	2 cut(s)	17, 1527
NciI	CCSGG	2 cut(s)	254, 1670
NcoI	CCATGG	2 cut(s)	828, 1489
NdeI	CATATG	1 cut(s)	494
NdeII	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
NlaIV	GGNNCC	6 cut(s)	726, 912, 1085, 1086, 1159, 1206
NmuCI	GTSAC	3 cut(s)	265, 594, 640
NspI	RCATGY	3 cut(s)	1054, 1283, 1504
PaeI	GCATGC	2 cut(s)	1054, 1283
PagI	TCATGA	1 cut(s)	781
PcsI	WCGNNNNNNNCGW	2 cut(s)	80, 1434
PfeI	GAWTC	8 cut(s)	80, 152, 766, 1333, 1622, 1658, 1673, 1685
PflFI	GACNNNGTC	2 cut(s)	1430, 1702
PflMI	CCANNNNNTGG	2 cut(s)	1040, 1714
PfoI	TCCNGGA	2 cut(s)	252, 1668
PkrI	GCNGC	4 cut(s)	22, 942, 1015, 1213
PleI	GAGTC	2 cut(s)	137, 857
PmaCI	CACGTG	1 cut(s)	361
PmlI	CACGTG	1 cut(s)	361
PpsI	GAGTC	2 cut(s)	137, 857
Ppu21I	YACGTR	1 cut(s)	361
PpuMI	RGGWCCY	2 cut(s)	1084, 1158
Psp5II	RGGWCCY	2 cut(s)	1084, 1158
Psp6I	CCWGG	2 cut(s)	554, 1132
PspCI	CACGTG	1 cut(s)	361
PspFI	CCCAGC	2 cut(s)	1079, 1297
PspGI	CCWGG	2 cut(s)	554, 1132
PspN4I	GGNNCC	6 cut(s)	726, 912, 1085, 1086, 1159, 1206
PspOMI	GGGCCC	1 cut(s)	1204
PspPI	GGNCC	9 cut(s)	292, 529, 552, 825, 1084, 1158, 1204, 1205, 1717
PspPPI	RGGWCCY	2 cut(s)	1084, 1158
PsyI	GACNNNGTC	2 cut(s)	1430, 1702
RsaI	GTAC	2 cut(s)	838, 851
RsaNI	GTAC	2 cut(s)	837, 850
RseI	CAYNNNNRTG	1 cut(s)	507
SaqAI	TTAA	4 cut(s)	669, 738, 894, 1149
SatI	GCNGC	4 cut(s)	21, 941, 1014, 1212
Sau3AI	GATC	8 cut(s)	137, 318, 427, 472, 1027, 1152, 1342, 1742
Sau96I	GGNCC	9 cut(s)	292, 529, 552, 825, 1084, 1158, 1204, 1205, 1717
SchI	GAGTC	2 cut(s)	137, 857
ScrFI	CCNGG	4 cut(s)	254, 556, 1134, 1670
SduI	GDGCHC	4 cut(s)	48, 1208, 1519, 1668
SfaNI	GCATC	3 cut(s)	499, 1129, 1447
SfcI	CTRYAG	1 cut(s)	1584
SinI	GGWCC	6 cut(s)	292, 529, 825, 1084, 1158, 1717
SmiMI	CAYNNNNRTG	1 cut(s)	507
SmlI	CTYRAG	1 cut(s)	1694
SmoI	CTYRAG	1 cut(s)	1694
SphI	GCATGC	2 cut(s)	1054, 1283
Sse9I	AATT	6 cut(s)	241, 578, 616, 752, 1338, 1710
SsiI	CCGC	5 cut(s)	48, 339, 742, 1271, 1519
SspI	AATATT	1 cut(s)	280
SspMI	CTAG	4 cut(s)	234, 431, 800, 860
StyD4I	CCNGG	4 cut(s)	252, 554, 1132, 1668
StyI	CCWWGG	2 cut(s)	828, 1489
TaaI	ACNGT	7 cut(s)	41, 600, 1147, 1327, 1569, 1702, 1721
TaiI	ACGT	3 cut(s)	363, 370, 791
TaqI	TCGA	2 cut(s)	83, 1437
TasI	AATT	6 cut(s)	241, 578, 616, 752, 1338, 1710
TfiI	GAWTC	8 cut(s)	80, 152, 766, 1333, 1622, 1658, 1673, 1685
Tru1I	TTAA	4 cut(s)	669, 738, 894, 1149
Tru9I	TTAA	4 cut(s)	669, 738, 894, 1149
TscAI	CASTG	5 cut(s)	46, 162, 274, 1150, 1458
TseFI	GTSAC	3 cut(s)	265, 594, 640
TseI	GCWGC	4 cut(s)	20, 940, 1013, 1211
Tsp45I	GTSAC	3 cut(s)	265, 594, 640
TspDTI	ATGAA	5 cut(s)	93, 636, 696, 770, 1162
TspRI	CASTG	5 cut(s)	46, 162, 274, 1150, 1458
Tth111I	GACNNNGTC	2 cut(s)	1430, 1702
Van91I	CCANNNNNTGG	2 cut(s)	1040, 1714
VpaK11BI	GGWCC	6 cut(s)	292, 529, 825, 1084, 1158, 1717
XagI	CCTNNNNNAGG	1 cut(s)	1616
XbaI	TCTAGA	1 cut(s)	430
XceI	RCATGY	3 cut(s)	1054, 1283, 1504
XcmI	CCANNNNNNNNNTGG	1 cut(s)	365
XspI	CTAG	4 cut(s)	234, 431, 800, 860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.