Rh1CG269200

Phosphatidylinositol 4-kinase gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0010706
Physical Location & Seq
Forward (+)
53454508 .. 53459031
4524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG269200.1

Sequence Viewer

Length: 1434 bp
ATGTCGTCGGCCGGTGTTGCCGCTCTCAGTCCGGTTCACTGTGAGCCGTTGCTCACTGGTGGTAACTGTCCTGCTATGTCGTGTATGTCGTCGAGTGAATCGATTTTGATATACCTGTCTGTTTCGGGATCGACTACTCCTATGAGTGTGTTGAAATCGGATTCGATTGAGTCTGTGAAGCTTAGGATTCAGACATATAAGGGGTTTGTTGTGAAGAAGCAGAAGCTAGTGTGTGGAGGGAGGGAACTGGCTCGGAGTAATTCGATACTGAGGGAATATGGAGTGTCGGATGGGAATGTGATGCATTTGGTTCTTAGGCTGTCTGATCTTCAGGAGATCAATGTTCGAACTAATTGTGGGAAAGAGTTAACGTTCCATGTGGAGCGTGGGAAAGATGTTGGGTATGTAAAGCAGAAGATTGCGAAGAATAGTAAAGAGTTTGTTGATCTAGAGGAGCAGGAAGTTGTGTTGGATGGAAAGCCACTTGAGGATCAGAAGCTTATTGATGACATATGTAAACAGAATGATGGTGTGTTACATTTGTTGGTTCGGAAGTCTGCAAAACTTCGTGCTAGACCTGTTGAGAAGAACTTTGAGTTGTCTATTGTGGCGTCACATGTGGATGATGGGAAAAAAGAAATTGATGGAGAGAATGTGAAGCAGCAATATGATGCTGGTGAGCAAGAGTTTAGCAGAGGTTACAAAGCTGATGAGATGATTGAGCCAAGAAAGCCGCTGGATAGAGAATTTGGGTTGGAGCCTGTCATTGTTAACCCCAAGATTGAATTGCCGTCTGTGATCTGGGATATGGTTGATGAAACAGTTGAAGGACTAGAGAGTGGGAATCACCCAATCAGGTCAATGGAGGGTACAGGAGGAGCATATTTTATGGTGGACTCATCTGGGAAAAAGTATGTGTCTGTGTTTAAGCCAATTGATGAGGAGCCTATGGCTGTGAACAATCCAAGAGGGCTTCCATTGTCATTGGATGGGGAAGGATTGAAAAAGGGGACAAGAGTTGGAGAAGGGGCTTTCAGAGAGGTTGCAGCTTACCTTTTGGATCATCCAAAGGGTGGGCGGCGTATGTTGTATGGTGATGGGAAAGGCTTTGCTGGAGTTCCACCAACATTCATAGTGAAGTGCTTTCATAGAGGATTCAACAACCCAGAAGATGTAATGGTCAAAATTGGTTCCTTACAGAAGTTCATGGAGAATGATGGAAGTTGTGAGGACATGGGCCCTGGTGCTTTCCCAACTGAGGAAGTGCATAAGATTTCTGTTTTGGACCTGAGATTGGCCAATGCGGACAGACACGCAGGAAATATCTTGCTGAGCAAAGAGGGTGAAGCTGGCCAGACTATGCTGATTCCCATTGACCACGGTTACTGCTTGCCAGAAAGTGTATGTTCTAAACTCGCTTTATCTTTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase ubiquitin

Protein Analysis

477

Amino Acids

52.31

Weight (kDa)

5.62

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 39 - 107 7.6e-12 Ubiquitin family
ubiquitin PF00240 113 - 185 1.1e-09 Ubiquitin family
PI3_PI4_kinase PF00454 291 - 467 2.7e-33 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1073
AccBSI CCGCTC 1 cut(s) 23
AciI CCGC 4 cut(s) 21, 734, 1078, 1304
AclI AACGTT 1 cut(s) 371
AclWI GGATC 3 cut(s) 136, 498, 1068
AcoI YGGCCR 3 cut(s) 9, 1296, 1351
AcsI RAATTY 1 cut(s) 746
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 611
AfaI GTAC 1 cut(s) 871
AfiI CCNNNNNNNGG 3 cut(s) 1073, 1258, 1294
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 5 cut(s) 154, 785, 827, 1003, 1159
AjnI CCWGG 1 cut(s) 1240
AluBI AGCT 6 cut(s) 181, 226, 499, 707, 1049, 1349
AluI AGCT 6 cut(s) 181, 226, 499, 707, 1049, 1349
AlwI GGATC 3 cut(s) 136, 498, 1068
AoxI GGCC 4 cut(s) 9, 1237, 1296, 1351
ApaI GGGCCC 1 cut(s) 1241
ApeKI GCWGC 2 cut(s) 661, 1046
ApoI RAATTY 1 cut(s) 746
AspS9I GGNCC 3 cut(s) 1237, 1238, 1285
AsuHPI GGTGA 4 cut(s) 689, 839, 1106, 1355
AsuII TTCGAA 1 cut(s) 346
AvaII GGWCC 1 cut(s) 1285
BaeGI GKGCMC 1 cut(s) 1241
BalI TGGCCA 2 cut(s) 1298, 1353
BanII GRGCYC 1 cut(s) 1241
BbvI GCAGC 2 cut(s) 673, 1058
BccI CCATC 8 cut(s) 284, 467, 521, 620, 638, 983, 1091, 1211
BceAI ACGGC 2 cut(s) 31, 775
BciT130I CCWGG 1 cut(s) 1242
BfaI CTAG 4 cut(s) 227, 449, 573, 833
BisI GCNGC 5 cut(s) 21, 662, 734, 1047, 1079
BlpI GCTNAGC 1 cut(s) 1331
BlsI GCNGC 5 cut(s) 22, 663, 735, 1048, 1080
Bme1390I CCNGG 1 cut(s) 1242
Bme18I GGWCC 1 cut(s) 1285
BmgT120I GGNCC 3 cut(s) 1237, 1238, 1285
BmiI GGNNCC 4 cut(s) 759, 945, 1192, 1239
BmrFI CCNGG 1 cut(s) 1242
BmsI GCATC 2 cut(s) 291, 661
BpmI CTGGAG 1 cut(s) 1134
Bpu10I CCTNAGC 1 cut(s) 182
Bpu1102I GCTNAGC 1 cut(s) 1331
Bpu14I TTCGAA 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 506
Bsa29I ATCGAT 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 1240, 1378
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 639, 669
Bsc4I CCNNNNNNNGG 3 cut(s) 1073, 1258, 1294
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 61, 252
BseBI CCWGG 1 cut(s) 1242
BseCI ATCGAT 1 cut(s) 101
BseDI CCNNGG 2 cut(s) 1240, 1378
BseGI GGATG 5 cut(s) 295, 478, 628, 994, 1063
BseLI CCNNNNNNNGG 3 cut(s) 1073, 1258, 1294
BseMII CTCAG 5 cut(s) 40, 260, 1248, 1280, 1322
BseNI ACTGG 2 cut(s) 61, 252
BseRI GAGGAG 3 cut(s) 467, 891, 956
BseSI GKGCMC 1 cut(s) 1241
BseX3I CGGCCG 1 cut(s) 9
BseXI GCAGC 2 cut(s) 673, 1058
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 11, 1239, 1298, 1353
BshVI ATCGAT 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 12
BsiSI CCGG 2 cut(s) 12, 32
BslFI GGGAC 1 cut(s) 1024
BslI CCNNNNNNNGG 3 cut(s) 1073, 1258, 1294
BsmFI GGGAC 1 cut(s) 1024
BsnI GGCC 4 cut(s) 11, 1239, 1298, 1353
Bsp119I TTCGAA 1 cut(s) 346
Bsp120I GGGCCC 1 cut(s) 1237
Bsp1286I GDGCHC 1 cut(s) 1241
Bsp143I GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
Bsp1720I GCTNAGC 1 cut(s) 1331
BspACI CCGC 4 cut(s) 21, 734, 1078, 1304
BspANI GGCC 4 cut(s) 11, 1239, 1298, 1353
BspCNI CTCAG 5 cut(s) 39, 261, 1249, 1281, 1323
BspDI ATCGAT 1 cut(s) 101
BspLI GGNNCC 4 cut(s) 759, 945, 1192, 1239
BspPI GGATC 3 cut(s) 136, 498, 1068
BspT104I TTCGAA 1 cut(s) 346
BsrBI CCGCTC 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 2 cut(s) 61, 252
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 1240, 1378
BssMI GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
BssNI GRCGYC 1 cut(s) 611
Bst2UI CCWGG 1 cut(s) 1242
Bst4CI ACNGT 4 cut(s) 41, 68, 823, 1382
BstACI GRCGYC 1 cut(s) 611
BstBI TTCGAA 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 1351, 1391
BstDEI CTNAG 7 cut(s) 26, 182, 269, 314, 1257, 1289, 1331
BstDSI CCRYGG 1 cut(s) 1378
BstF5I GGATG 5 cut(s) 295, 478, 628, 994, 1063
BstKTI GATC 7 cut(s) 131, 328, 339, 448, 493, 801, 1063
BstMBI GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 17, 730
BstNI CCWGG 1 cut(s) 1242
BstNSI RCATGY 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 1240
BstSLI GKGCMC 1 cut(s) 1241
BstV1I GCAGC 2 cut(s) 673, 1058
BstZI CGGCCG 1 cut(s) 9
Bsu15I ATCGAT 1 cut(s) 101
BsuRI GGCC 4 cut(s) 11, 1239, 1298, 1353
BsuTUI ATCGAT 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 1378
BtsCI GGATG 5 cut(s) 295, 478, 628, 994, 1063
BtsIMutI CAGTG 2 cut(s) 37, 54
Cac8I GCNNGC 2 cut(s) 1351, 1391
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 3 cut(s) 1237, 1238, 1285
ClaI ATCGAT 1 cut(s) 101
CseI GACGC 1 cut(s) 600
Csp6I GTAC 1 cut(s) 870
CviAII CATG 4 cut(s) 377, 617, 1207, 1234
CviQI GTAC 1 cut(s) 870
DdeI CTNAG 7 cut(s) 26, 182, 269, 314, 1257, 1289, 1331
DpnI GATC 7 cut(s) 130, 327, 338, 447, 492, 800, 1062
DpnII GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
EaeI YGGCCR 3 cut(s) 9, 1296, 1351
EagI CGGCCG 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 1241
Eco47I GGWCC 1 cut(s) 1285
Eco52I CGGCCG 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 1238
EcoRII CCWGG 1 cut(s) 1240
EcoT22I ATGCAT 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 1241
FaeI CATG 4 cut(s) 380, 620, 1210, 1237
FaqI GGGAC 1 cut(s) 1024
FatI CATG 4 cut(s) 376, 616, 1206, 1233
FauNDI CATATG 1 cut(s) 512
Fnu4HI GCNGC 5 cut(s) 21, 662, 734, 1047, 1079
FokI GGATG 5 cut(s) 302, 485, 635, 1001, 1050
FriOI GRGCYC 1 cut(s) 1241
Fsp4HI GCNGC 5 cut(s) 21, 662, 734, 1047, 1079
FspBI CTAG 4 cut(s) 227, 449, 573, 833
GluI GCNGC 5 cut(s) 21, 662, 734, 1047, 1079
GsuI CTGGAG 1 cut(s) 1134
HaeIII GGCC 4 cut(s) 11, 1239, 1298, 1353
HapII CCGG 2 cut(s) 12, 32
HgaI GACGC 1 cut(s) 600
Hin1I GRCGYC 1 cut(s) 611
Hin1II CATG 4 cut(s) 380, 620, 1210, 1237
HincII GTYRAC 2 cut(s) 369, 772
HindII GTYRAC 2 cut(s) 369, 772
HindIII AAGCTT 2 cut(s) 179, 497
HinfI GANTC 8 cut(s) 98, 161, 170, 187, 844, 896, 1155, 1366
HpaI GTTAAC 2 cut(s) 369, 772
HpaII CCGG 2 cut(s) 12, 32
HphI GGTGA 4 cut(s) 689, 839, 1106, 1355
Hpy166II GTNNAC 6 cut(s) 37, 369, 518, 772, 895, 958
Hpy188I TCNGA 8 cut(s) 160, 192, 255, 289, 325, 495, 552, 1037
Hpy188III TCNNGA 3 cut(s) 126, 332, 449
Hpy8I GTNNAC 6 cut(s) 37, 369, 518, 772, 895, 958
Hpy99I CGWCG 2 cut(s) 10, 94
HpyAV CCTTC 3 cut(s) 821, 989, 1019
HpyCH4III ACNGT 4 cut(s) 41, 68, 823, 1382
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 4 cut(s) 304, 560, 1046, 1267
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 730
HpyF3I CTNAG 7 cut(s) 26, 182, 269, 314, 1257, 1289, 1331
HpySE526I ACGT 1 cut(s) 371
Hsp92I GRCGYC 1 cut(s) 611
Hsp92II CATG 4 cut(s) 380, 620, 1210, 1237
KspAI GTTAAC 2 cut(s) 369, 772
Kzo9I GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
LmnI GCTCC 5 cut(s) 382, 454, 757, 878, 943
Lsp1109I GCAGC 2 cut(s) 673, 1058
LweI GCATC 2 cut(s) 291, 661
MaeI CTAG 4 cut(s) 227, 449, 573, 833
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 5 cut(s) 62, 534, 612, 698, 1382
MalI GATC 7 cut(s) 130, 327, 338, 447, 492, 800, 1062
MbiI CCGCTC 1 cut(s) 23
MboI GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
MboII GAAGA 6 cut(s) 226, 320, 427, 436, 598, 1181
MfeI CAATTG 1 cut(s) 933
MhlI GDGCHC 1 cut(s) 1241
MlsI TGGCCA 2 cut(s) 1298, 1353
MluCI AATT 7 cut(s) 259, 352, 639, 746, 785, 933, 1185
MluNI TGGCCA 2 cut(s) 1298, 1353
MlyI GAGTC 2 cut(s) 179, 890
MmeI TCCRAC 4 cut(s) 267, 450, 735, 1000
Mox20I TGGCCA 2 cut(s) 1298, 1353
Mph1103I ATGCAT 1 cut(s) 306
MscI TGGCCA 2 cut(s) 1298, 1353
MseI TTAA 3 cut(s) 368, 771, 927
MslI CAYNNNNRTG 1 cut(s) 621
Msp20I TGGCCA 2 cut(s) 1298, 1353
MspA1I CMGCKG 1 cut(s) 736
MspI CCGG 2 cut(s) 12, 32
MspR9I CCNGG 1 cut(s) 1242
MunI CAATTG 1 cut(s) 933
MvaI CCWGG 1 cut(s) 1242
MwoI GCNNNNNNNGC 2 cut(s) 17, 730
NdeI CATATG 1 cut(s) 512
NdeII GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
NlaIII CATG 4 cut(s) 380, 620, 1210, 1237
NlaIV GGNNCC 4 cut(s) 759, 945, 1192, 1239
NmuCI GTSAC 1 cut(s) 612
NsiI ATGCAT 1 cut(s) 306
NspI RCATGY 1 cut(s) 620
NspV TTCGAA 1 cut(s) 346
PciI ACATGT 1 cut(s) 616
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 98
PfeI GAWTC 6 cut(s) 98, 161, 187, 844, 1155, 1366
PflMI CCANNNNNTGG 1 cut(s) 1073
PkrI GCNGC 5 cut(s) 22, 663, 735, 1048, 1080
PleI GAGTC 2 cut(s) 178, 890
PpsI GAGTC 2 cut(s) 178, 890
PscI ACATGT 1 cut(s) 616
Psp1406I AACGTT 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 1240
PspGI CCWGG 1 cut(s) 1240
PspN4I GGNNCC 4 cut(s) 759, 945, 1192, 1239
PspOMI GGGCCC 1 cut(s) 1237
PspPI GGNCC 3 cut(s) 1237, 1238, 1285
RsaI GTAC 1 cut(s) 871
RsaNI GTAC 1 cut(s) 870
RseI CAYNNNNRTG 1 cut(s) 621
SaqAI TTAA 3 cut(s) 368, 771, 927
SatI GCNGC 5 cut(s) 21, 662, 734, 1047, 1079
Sau3AI GATC 7 cut(s) 128, 325, 336, 445, 490, 798, 1060
Sau96I GGNCC 3 cut(s) 1237, 1238, 1285
SchI GAGTC 2 cut(s) 179, 890
ScrFI CCNGG 1 cut(s) 1242
SduI GDGCHC 1 cut(s) 1241
SfaNI GCATC 2 cut(s) 291, 661
SfuI TTCGAA 1 cut(s) 346
SinI GGWCC 1 cut(s) 1285
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 7 cut(s) 259, 352, 639, 746, 785, 933, 1185
SsiI CCGC 4 cut(s) 21, 734, 1078, 1304
SspMI CTAG 4 cut(s) 227, 449, 573, 833
StyD4I CCNGG 1 cut(s) 1240
TaaI ACNGT 4 cut(s) 41, 68, 823, 1382
TaiI ACGT 1 cut(s) 374
TaqI TCGA 6 cut(s) 92, 101, 131, 164, 263, 346
TasI AATT 7 cut(s) 259, 352, 639, 746, 785, 933, 1185
TauI GCSGC 3 cut(s) 23, 736, 1081
TfiI GAWTC 6 cut(s) 98, 161, 187, 844, 1155, 1366
Tru1I TTAA 3 cut(s) 368, 771, 927
Tru9I TTAA 3 cut(s) 368, 771, 927
TscAI CASTG 2 cut(s) 44, 61
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 2 cut(s) 661, 1046
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 4 cut(s) 831, 1120, 1136, 1195
TspRI CASTG 2 cut(s) 44, 61
Van91I CCANNNNNTGG 1 cut(s) 1073
VpaK11BI GGWCC 1 cut(s) 1285
XapI RAATTY 1 cut(s) 746
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 1 cut(s) 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XspI CTAG 4 cut(s) 227, 449, 573, 833
Zsp2I ATGCAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.