MD07G1160100.v1.1

Pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0020397
Physical Location & Seq
Reverse (-)
23394508 .. 23395137
630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1160100.v1.1.491

Sequence Viewer

Length: 630 bp
ATGTTGAACAAAGGGTTCGTGCCGGATACGCTGAGTTACACGACATTGTTGAATAGTTTGTGTAGGAAGAAGAAGCTTCGTGAGGCGTATAGGCTGCTTTGTAGGATGAAGGTTAAGGGTTGCAATCCTGATATTGTGCATTATAACACTGTTATATTGGGGTTCTGTAGAGAGGGGCGTCCGGGGGATGCTTGTAAGGTTCTCGAGGACATGGCGTCAAATGGGTGCTTGCCAAATTTGGTATCTTATCGGACCTTGGTTAGTGGGTTATGCGATCATGGGATGCTTGACGAGGCTAAGAGTTTGGAGACAATGGTATCAAAGGGGTTTTCTCCGCATTTCTCAGTTATTCATGCTTTGGTTAAAGGGTTCTGTAATGTGGGTACGTTTGAGGAAGCTTGTGGAGTTTTGGAGGAGGTGCTAAACCACGGGGAAGTTCCTCATATGGATATTTGGATTACGGTAGTTCCTGGGATATGCGAAGAGATAGAGCTGGTGAGAATGGAGGAAATTCTGAGAGAGGTAATGAAGGTGGAAATTAGGCCAAACACTAGAATTGTGGAGGCAGCGTTTGGTTTGGAGGATTACTTGATCATAAAGATACAGGCTAACCCTAGGAGAGTTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

210

Amino Acids

23.55

Weight (kDa)

6.32

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 1 - 56 1.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 4 - 37 3.8e-10 PPR repeat
PPR_2 PF13041 8 - 45 6.9e-11 PPR repeat family
PPR PF01535 12 - 41 2.6e-07 PPR repeat
PPR_long PF17177 23 - 139 4.8e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 39 - 71 3.6e-12 PPR repeat
PPR_2 PF13041 43 - 92 2e-16 PPR repeat family
PPR PF01535 46 - 76 6.3e-07 PPR repeat
PPR_1 PF12854 74 - 102 4e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020397)

Species Orthologous Gene IDs
malus_domestica MD07G1160100.v1.1
pyrus_communis pycom07g15640
rosa_chinensis RchiOBHm_Chr2g0097111
rosa_samantha Rh2BG113400 Rh2CG115700 Rh2DG114700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 144
AciI CCGC 1 cut(s) 335
AcsI RAATTY 2 cut(s) 235, 510
AcyI GRCGYC 2 cut(s) 178, 215
AfaI GTAC 1 cut(s) 385
AgsI TTSAA 2 cut(s) 7, 52
AhdI GACNNNNNGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 3 cut(s) 76, 398, 493
AluI AGCT 3 cut(s) 76, 398, 493
Alw26I GTCTC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 2 cut(s) 94, 566
ApoI RAATTY 2 cut(s) 235, 510
AspA2I CCTAGG 1 cut(s) 614
AspS9I GGNCC 1 cut(s) 252
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 508
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 252
AvrII CCTAGG 1 cut(s) 614
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BbvI GCAGC 2 cut(s) 81, 578
BciT130I CCWGG 1 cut(s) 471
BciVI GTATCC 1 cut(s) 19
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 2 cut(s) 552, 615
BfmI CTRYAG 1 cut(s) 166
BfuI GTATCC 1 cut(s) 19
BisI GCNGC 2 cut(s) 95, 567
BlnI CCTAGG 1 cut(s) 614
BlsI GCNGC 2 cut(s) 96, 568
Bme1390I CCNGG 2 cut(s) 183, 471
Bme18I GGWCC 1 cut(s) 252
BmeRI GACNNNNNGTC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 252
BmrFI CCNGG 2 cut(s) 183, 471
BmsI GCATC 2 cut(s) 178, 273
BpuMI CCSGG 1 cut(s) 183
BsaHI GRCGYC 2 cut(s) 178, 215
BsaJI CCNNGG 5 cut(s) 182, 255, 427, 470, 614
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 5 cut(s) 182, 255, 427, 470, 614
BseGI GGATG 3 cut(s) 111, 193, 288
BseMII CTCAG 3 cut(s) 23, 357, 506
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 2 cut(s) 81, 578
BshFI GGCC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 2 cut(s) 23, 182
BsmAI GTCTC 1 cut(s) 302
BsnI GGCC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 203
Bsp143I GATC 2 cut(s) 274, 591
BspACI CCGC 1 cut(s) 335
BspANI GGCC 1 cut(s) 544
BspCNI CTCAG 3 cut(s) 24, 356, 507
BssECI CCNNGG 5 cut(s) 182, 255, 427, 470, 614
BssMI GATC 2 cut(s) 274, 591
BssNI GRCGYC 2 cut(s) 178, 215
BssT1I CCWWGG 2 cut(s) 255, 614
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 2 cut(s) 151, 463
Bst6I CTCTTC 1 cut(s) 477
BstACI GRCGYC 2 cut(s) 178, 215
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 4 cut(s) 32, 297, 343, 515
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 3 cut(s) 111, 193, 288
BstKTI GATC 2 cut(s) 277, 594
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 2 cut(s) 274, 591
BstMWI GCNNNNNNNGC 1 cut(s) 28
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 181, 469
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 81, 578
BsuI GTATCC 1 cut(s) 19
BsuRI GGCC 1 cut(s) 544
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 3 cut(s) 111, 193, 288
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 252
CseI GACGC 2 cut(s) 167, 204
Csp6I GTAC 1 cut(s) 384
CviAII CATG 3 cut(s) 211, 278, 353
CviJI RGCY 7 cut(s) 76, 94, 296, 398, 493, 544, 608
CviKI_1 RGCY 7 cut(s) 76, 94, 296, 398, 493, 544, 608
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 4 cut(s) 32, 297, 343, 515
DpnI GATC 2 cut(s) 276, 593
DpnII GATC 2 cut(s) 274, 591
DriI GACNNNNNGTC 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 477
Eam1105I GACNNNNNGTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 255, 614
Eco47I GGWCC 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 2 cut(s) 255, 614
ErhI CCWWGG 2 cut(s) 255, 614
FaeI CATG 3 cut(s) 214, 281, 356
FatI CATG 3 cut(s) 210, 277, 352
FauNDI CATATG 1 cut(s) 444
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 2 cut(s) 95, 567
FokI GGATG 3 cut(s) 118, 200, 295
Fsp4HI GCNGC 2 cut(s) 95, 567
FspBI CTAG 2 cut(s) 552, 615
GluI GCNGC 2 cut(s) 95, 567
HaeIII GGCC 1 cut(s) 544
HapII CCGG 2 cut(s) 23, 182
HgaI GACGC 2 cut(s) 167, 204
Hin1I GRCGYC 2 cut(s) 178, 215
Hin1II CATG 3 cut(s) 214, 281, 356
HindIII AAGCTT 2 cut(s) 74, 396
HpaII CCGG 2 cut(s) 23, 182
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 2 cut(s) 252, 516
Hpy188III TCNNGA 3 cut(s) 80, 128, 203
Hpy8I GTNNAC 1 cut(s) 625
HpyAV CCTTC 2 cut(s) 103, 523
HpyCH4III ACNGT 2 cut(s) 151, 463
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 2 cut(s) 123, 139
HpyF10VI GCNNNNNNNGC 1 cut(s) 28
HpyF3I CTNAG 4 cut(s) 32, 297, 343, 515
HpySE526I ACGT 1 cut(s) 386
Hsp92I GRCGYC 2 cut(s) 178, 215
Hsp92II CATG 3 cut(s) 214, 281, 356
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 2 cut(s) 274, 591
LpnPI CCDG 7 cut(s) 36, 141, 195, 456, 479, 483, 590
Lsp1109I GCAGC 2 cut(s) 81, 578
LweI GCATC 2 cut(s) 178, 273
MaeI CTAG 2 cut(s) 552, 615
MaeII ACGT 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 35
MalI GATC 2 cut(s) 276, 593
MboI GATC 2 cut(s) 274, 591
MboII GAAGA 3 cut(s) 79, 82, 494
MluCI AATT 4 cut(s) 235, 510, 537, 555
MseI TTAA 2 cut(s) 114, 363
MspI CCGG 2 cut(s) 23, 182
MspR9I CCNGG 2 cut(s) 183, 471
MvaI CCWGG 1 cut(s) 471
MwoI GCNNNNNNNGC 1 cut(s) 28
NciI CCSGG 1 cut(s) 183
NdeI CATATG 1 cut(s) 444
NdeII GATC 2 cut(s) 274, 591
NlaIII CATG 3 cut(s) 214, 281, 356
PaeR7I CTCGAG 1 cut(s) 203
PkrI GCNGC 2 cut(s) 96, 568
PsiI TTATAA 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspPI GGNCC 1 cut(s) 252
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SaqAI TTAA 2 cut(s) 114, 363
SatI GCNGC 2 cut(s) 95, 567
Sau3AI GATC 2 cut(s) 274, 591
Sau96I GGNCC 1 cut(s) 252
ScrFI CCNGG 2 cut(s) 183, 471
SfaNI GCATC 2 cut(s) 178, 273
SfcI CTRYAG 1 cut(s) 166
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 1 cut(s) 252
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 4 cut(s) 235, 510, 537, 555
SsiI CCGC 1 cut(s) 335
SspMI CTAG 2 cut(s) 552, 615
StyD4I CCNGG 2 cut(s) 181, 469
StyI CCWWGG 2 cut(s) 255, 614
TaaI ACNGT 2 cut(s) 151, 463
TaiI ACGT 1 cut(s) 389
TaqI TCGA 1 cut(s) 204
TasI AATT 4 cut(s) 235, 510, 537, 555
Tru1I TTAA 2 cut(s) 114, 363
Tru9I TTAA 2 cut(s) 114, 363
TscAI CASTG 1 cut(s) 154
TseI GCWGC 2 cut(s) 94, 566
TspDTI ATGAA 3 cut(s) 122, 341, 542
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 252
XapI RAATTY 2 cut(s) 235, 510
XhoI CTCGAG 1 cut(s) 203
XmaJI CCTAGG 1 cut(s) 614
XspI CTAG 2 cut(s) 552, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.