Rh2DG114700

Pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0020397
Physical Location & Seq
Forward (+)
9482234 .. 9482866
633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG114700.1

Sequence Viewer

Length: 633 bp
ATGATCAACAAATGGTTTGTTCCGGATTCGTTGAGTTATACTAGCTTGTTGAATAGCTTGTGTAGGAAGAAACAGCTGCGTGAGGCGTATAAGCTTCTTCGTAGGATGAAGGTGAGGGGGTGTAACCCTGACATTGTGCATTACAATACTATAATATTGGGGTTTTGTAGAGAAGGGAGGACAGTGGATGCTTGTAAGGTTCTTGAGGATATGGCATCCAATGGGTGCTTGCCAAATTTGGTGTCGTATAGGACTTTGGTTAGTGGGTTGTCTGATCAGGGGATGCTTGATGAGGCGAAGAACTATATGGAGACAATGATATCAAAGGGGTTTTCTCCACATTTTTCGGTAATCCATGCTTTGGTTAAGGGGTTCTGCAATGTGGGTAGGGTCGAGGAAGCTGGTGGAGTTATGGAGGAGATACCAAGTCATGGGGAGGTTCCGCACAGGGAGACTTGGTTGACAATAGTTCCGGGTATATGCGAAGAGGTTGAGCTGGTGAGAATGGAAGAAGTTCCGAAGGAAATAATGAAGGTAGAGATCAAGCCTAGCACCAGAATAGTGGAGGCAGCTTTTGGATTGGAGAACTACTTGATCAAAAAAATGCATGCTAGTAAATCAAGGAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

210

Amino Acids

23.76

Weight (kDa)

8.44

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 7 - 37 1.6e-08 PPR repeat
PPR_2 PF13041 8 - 45 1.4e-10 PPR repeat family
PPR_3 PF13812 8 - 52 7.5e-08 Pentatricopeptide repeat domain
PPR PF01535 12 - 41 1.2e-07 PPR repeat
PPR_long PF17177 24 - 156 1.8e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 39 - 71 6.9e-13 PPR repeat
PPR_2 PF13041 43 - 91 4.7e-15 PPR repeat family
PPR_3 PF13812 43 - 87 2.2e-06 Pentatricopeptide repeat domain
PPR PF01535 46 - 76 1.8e-06 PPR repeat
PPR_1 PF12854 74 - 106 2.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020397)

Species Orthologous Gene IDs
malus_domestica MD07G1160100.v1.1
pyrus_communis pycom07g15640
rosa_chinensis RchiOBHm_Chr2g0097111
rosa_samantha Rh2BG113400 Rh2CG115700 Rh2DG114700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 361, 431
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 1 cut(s) 443
AcsI RAATTY 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 361, 431
AgsI TTSAA 1 cut(s) 52
AluBI AGCT 7 cut(s) 45, 57, 76, 94, 401, 496, 572
AluI AGCT 7 cut(s) 45, 57, 76, 94, 401, 496, 572
Alw26I GTCTC 2 cut(s) 305, 446
Aor13HI TCCGGA 1 cut(s) 22
ApeKI GCWGC 2 cut(s) 76, 569
ApoI RAATTY 1 cut(s) 235
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
Asp700I GAANNNNTTC 1 cut(s) 513
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 2 cut(s) 124, 511
BbvI GCAGC 2 cut(s) 63, 581
BclI TGATCA 3 cut(s) 3, 274, 594
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 305, 446
BfaI CTAG 3 cut(s) 42, 549, 612
BisI GCNGC 2 cut(s) 77, 570
BlsI GCNGC 2 cut(s) 78, 571
Bme1390I CCNGG 1 cut(s) 474
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 1 cut(s) 474
BmsI GCATC 3 cut(s) 178, 224, 273
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 431
Bse3DI GCAATG 1 cut(s) 385
BseAI TCCGGA 1 cut(s) 22
BseGI GGATG 4 cut(s) 111, 193, 215, 288
BseLI CCNNNNNNNGG 2 cut(s) 361, 431
BseMI GCAATG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 2 cut(s) 63, 581
BsiSI CCGG 2 cut(s) 23, 473
BslI CCNNNNNNNGG 2 cut(s) 361, 431
BsmAI GTCTC 2 cut(s) 305, 446
Bsp13I TCCGGA 1 cut(s) 22
Bsp143I GATC 4 cut(s) 3, 274, 540, 594
BspACI CCGC 1 cut(s) 443
BspEI TCCGGA 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 385
BssMI GATC 4 cut(s) 3, 274, 540, 594
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 230, 609
BstF5I GGATG 4 cut(s) 111, 193, 215, 288
BstKTI GATC 4 cut(s) 6, 277, 543, 597
BstMAI GTCTC 2 cut(s) 305, 446
BstMBI GATC 4 cut(s) 3, 274, 540, 594
BstNSI RCATGY 1 cut(s) 611
BstSCI CCNGG 1 cut(s) 472
BstV1I GCAGC 2 cut(s) 63, 581
BstXI CCANNNNNNTGG 1 cut(s) 562
BtsCI GGATG 4 cut(s) 111, 193, 215, 288
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 230, 609
CviAII CATG 3 cut(s) 356, 431, 608
CviJI RGCY 8 cut(s) 45, 57, 76, 94, 401, 496, 547, 572
CviKI_1 RGCY 8 cut(s) 45, 57, 76, 94, 401, 496, 547, 572
DpnI GATC 4 cut(s) 5, 276, 542, 596
DpnII GATC 4 cut(s) 3, 274, 540, 594
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
Eco32I GATATC 1 cut(s) 321
EcoRV GATATC 1 cut(s) 321
EcoT22I ATGCAT 1 cut(s) 609
FaeI CATG 3 cut(s) 359, 434, 611
FatI CATG 3 cut(s) 355, 430, 607
FbaI TGATCA 3 cut(s) 3, 274, 594
Fnu4HI GCNGC 2 cut(s) 77, 570
FokI GGATG 4 cut(s) 118, 200, 202, 295
Fsp4HI GCNGC 2 cut(s) 77, 570
FspBI CTAG 3 cut(s) 42, 549, 612
GluI GCNGC 2 cut(s) 77, 570
HapII CCGG 2 cut(s) 23, 473
Hin1II CATG 3 cut(s) 359, 434, 611
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 1 cut(s) 26
HpaII CCGG 2 cut(s) 23, 473
HphI GGTGA 2 cut(s) 124, 511
Hpy166II GTNNAC 1 cut(s) 462
Hpy188I TCNGA 2 cut(s) 274, 519
Hpy188III TCNNGA 2 cut(s) 23, 203
Hpy8I GTNNAC 1 cut(s) 462
HpyAV CCTTC 4 cut(s) 103, 167, 514, 526
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 139, 378, 607
Hsp92II CATG 3 cut(s) 359, 434, 611
Kpn2I TCCGGA 1 cut(s) 22
Ksp22I TGATCA 3 cut(s) 3, 274, 594
Kzo9I GATC 4 cut(s) 3, 274, 540, 594
LpnPI CCDG 8 cut(s) 36, 141, 263, 387, 433, 482, 486, 568
Lsp1109I GCAGC 2 cut(s) 63, 581
LweI GCATC 3 cut(s) 178, 224, 273
MaeI CTAG 3 cut(s) 42, 549, 612
MaeIII GTNAC 1 cut(s) 122
MalI GATC 4 cut(s) 5, 276, 542, 596
MboI GATC 4 cut(s) 3, 274, 540, 594
MboII GAAGA 5 cut(s) 79, 89, 310, 497, 521
MluCI AATT 1 cut(s) 235
Mph1103I ATGCAT 1 cut(s) 609
MroI TCCGGA 1 cut(s) 22
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 2 cut(s) 23, 473
MspR9I CCNGG 1 cut(s) 474
NciI CCSGG 1 cut(s) 474
NdeII GATC 4 cut(s) 3, 274, 540, 594
NlaIII CATG 3 cut(s) 359, 434, 611
NlaIV GGNNCC 1 cut(s) 441
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 1 cut(s) 611
PaeI GCATGC 1 cut(s) 611
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 26
PflMI CCANNNNNTGG 2 cut(s) 361, 431
PkrI GCNGC 2 cut(s) 78, 571
PspN4I GGNNCC 1 cut(s) 441
PvuII CAGCTG 1 cut(s) 76
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 2 cut(s) 77, 570
Sau3AI GATC 4 cut(s) 3, 274, 540, 594
ScrFI CCNGG 1 cut(s) 474
SfaNI GCATC 3 cut(s) 178, 224, 273
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SphI GCATGC 1 cut(s) 611
Sse9I AATT 1 cut(s) 235
SsiI CCGC 1 cut(s) 443
SspI AATATT 1 cut(s) 156
SspMI CTAG 3 cut(s) 42, 549, 612
StyD4I CCNGG 1 cut(s) 472
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 393
TasI AATT 1 cut(s) 235
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 1 cut(s) 189
TseI GCWGC 2 cut(s) 76, 569
TspDTI ATGAA 2 cut(s) 122, 545
TspRI CASTG 1 cut(s) 189
Van91I CCANNNNNTGG 2 cut(s) 361, 431
XapI RAATTY 1 cut(s) 235
XceI RCATGY 1 cut(s) 611
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 3 cut(s) 42, 549, 612
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.