Rh2CG115700

Pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0020397
Physical Location & Seq
Forward (+)
10177280 .. 10179047
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG115700.1

Sequence Viewer

Length: 690 bp
ATGCAGGGGCTGTGCAGGAAGGGGCAGATGAACACTGCTGTGGATTTTCTGGAGGATATGATTAAGAAAGGGTTTGTTCCGGATTCGTTGAGTTATACTAGCTTGTTGAATAGCTTGTGTAGGAAGAAACAGCTGCGTGAGGCGTATAAGCTTCTTTGTAGGATGAAGGTGAAGGGGTGTAACCCAGACATTGTGCATTACAATACTGTGATATTGGGGTTTTGCAGAGAAGGGAAGGCAGTGGATGCTTGTAAGGTTCTTGAGGATATGGCATCCAATGGGTGCTTGCCAAATTTGGTGTCGTATAGGACTTTGGTTAGTGGGTTGTCTGATCAGGGGATGCTTGATGAGGCGAAGAACTATATGGAGACAATGATATCAAAGGGGTTTTCTCCACATTTTTCGGTTATCCATGCTTTGGTTAAGGGGTTCTACAATGTGGGTAGGGTCGAGGAAGCTTGTGGAGTTATGGAGGAGATACTAAGGCATGGGGAGGTTCCGCACAGGGAGACTTGGTTGACAATAGTTCCGGGGATATGCGAAGAGGTTGAGCTGGTGAGAATGGAAGAAGTTCTGAAGGAAATAATGAAGGTAGAGATCAAGCCTAGCACCAGAATAGTGGAGGCAGCTTTTGGATTGGAGAACTACTTGATCAAAAAAATGCATGCTAGTAAATCAAGGAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

229

Amino Acids

25.84

Weight (kDa)

8.32

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 1 - 41 1.7e-12 PPR repeat family
PPR_long PF17177 6 - 175 3.5e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 17 - 75 5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 23 - 56 6.3e-09 PPR repeat
PPR_2 PF13041 27 - 64 2.2e-10 PPR repeat family
PPR PF01535 31 - 60 2.1e-07 PPR repeat
PPR_1 PF12854 58 - 90 9e-12 PPR repeat
PPR_2 PF13041 62 - 110 1.1e-14 PPR repeat family
PPR_3 PF13812 62 - 106 9.5e-07 Pentatricopeptide repeat domain
PPR PF01535 65 - 95 2.3e-06 PPR repeat
PPR_1 PF12854 93 - 125 2.7e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020397)

Species Orthologous Gene IDs
malus_domestica MD07G1160100.v1.1
pyrus_communis pycom07g15640
rosa_chinensis RchiOBHm_Chr2g0097111
rosa_samantha Rh2BG113400 Rh2CG115700 Rh2DG114700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 418
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 1 cut(s) 500
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 596
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 1 cut(s) 109
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 7 cut(s) 102, 114, 133, 151, 458, 553, 629
AluI AGCT 7 cut(s) 102, 114, 133, 151, 458, 553, 629
Alw26I GTCTC 2 cut(s) 362, 503
AlwNI CAGNNNCTG 1 cut(s) 10
Aor13HI TCCGGA 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 133, 626
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp700I GAANNNNTTC 1 cut(s) 570
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 2 cut(s) 181, 568
BbvI GCAGC 2 cut(s) 120, 638
BclI TGATCA 2 cut(s) 331, 651
BcnI CCSGG 1 cut(s) 531
BcoDI GTCTC 2 cut(s) 362, 503
BfaI CTAG 3 cut(s) 99, 606, 669
BisI GCNGC 2 cut(s) 134, 627
BlsI GCNGC 2 cut(s) 135, 628
Bme1390I CCNGG 1 cut(s) 531
BmiI GGNNCC 1 cut(s) 498
BmrFI CCNGG 1 cut(s) 531
BmsI GCATC 3 cut(s) 235, 281, 330
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 531
BsaJI CCNNGG 1 cut(s) 530
BsaWI WCCGGW 1 cut(s) 79
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 418
BseAI TCCGGA 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 530
BseGI GGATG 4 cut(s) 168, 250, 272, 345
BseLI CCNNNNNNNGG 1 cut(s) 418
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 2 cut(s) 120, 638
BsgI GTGCAG 1 cut(s) 34
BsiSI CCGG 2 cut(s) 80, 530
BslI CCNNNNNNNGG 1 cut(s) 418
BsmAI GTCTC 2 cut(s) 362, 503
Bsp13I TCCGGA 1 cut(s) 79
Bsp143I GATC 3 cut(s) 331, 597, 651
BspACI CCGC 1 cut(s) 500
BspEI TCCGGA 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 498
BssECI CCNNGG 1 cut(s) 530
BssMI GATC 3 cut(s) 331, 597, 651
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 287, 666
BstDEI CTNAG 1 cut(s) 482
BstF5I GGATG 4 cut(s) 168, 250, 272, 345
BstKTI GATC 3 cut(s) 334, 600, 654
BstMAI GTCTC 2 cut(s) 362, 503
BstMBI GATC 3 cut(s) 331, 597, 651
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 1 cut(s) 529
BstV1I GCAGC 2 cut(s) 120, 638
BstXI CCANNNNNNTGG 1 cut(s) 619
BtsCI GGATG 4 cut(s) 168, 250, 272, 345
BtsI GCAGTG 2 cut(s) 33, 246
BtsIMutI CAGTG 2 cut(s) 33, 246
Cac8I GCNNGC 2 cut(s) 287, 666
CaiI CAGNNNCTG 1 cut(s) 10
CviAII CATG 3 cut(s) 413, 488, 665
CviJI RGCY 9 cut(s) 10, 102, 114, 133, 151, 458, 553, 604, 629
CviKI_1 RGCY 9 cut(s) 10, 102, 114, 133, 151, 458, 553, 604, 629
DdeI CTNAG 1 cut(s) 482
DpnI GATC 3 cut(s) 333, 599, 653
DpnII GATC 3 cut(s) 331, 597, 651
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco32I GATATC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 596
EcoRV GATATC 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 666
FaeI CATG 3 cut(s) 416, 491, 668
FatI CATG 3 cut(s) 412, 487, 664
FbaI TGATCA 2 cut(s) 331, 651
Fnu4HI GCNGC 2 cut(s) 134, 627
FokI GGATG 4 cut(s) 175, 257, 259, 352
Fsp4HI GCNGC 2 cut(s) 134, 627
FspBI CTAG 3 cut(s) 99, 606, 669
GluI GCNGC 2 cut(s) 134, 627
GsuI CTGGAG 1 cut(s) 71
HapII CCGG 2 cut(s) 80, 530
Hin1II CATG 3 cut(s) 416, 491, 668
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HindIII AAGCTT 2 cut(s) 149, 456
HinfI GANTC 1 cut(s) 83
HpaII CCGG 2 cut(s) 80, 530
HphI GGTGA 2 cut(s) 181, 568
Hpy166II GTNNAC 1 cut(s) 519
Hpy188I TCNGA 2 cut(s) 331, 576
Hpy188III TCNNGA 3 cut(s) 50, 80, 260
Hpy8I GTNNAC 1 cut(s) 519
HpyAV CCTTC 7 cut(s) 13, 160, 166, 224, 229, 571, 583
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4V TGCA 5 cut(s) 4, 15, 196, 225, 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 482
Hsp92II CATG 3 cut(s) 416, 491, 668
Kpn2I TCCGGA 1 cut(s) 79
Ksp22I TGATCA 2 cut(s) 331, 651
Kzo9I GATC 3 cut(s) 331, 597, 651
LpnPI CCDG 8 cut(s) 35, 93, 198, 320, 490, 539, 543, 625
Lsp1109I GCAGC 2 cut(s) 120, 638
LweI GCATC 3 cut(s) 235, 281, 330
MaeI CTAG 3 cut(s) 99, 606, 669
MaeIII GTNAC 1 cut(s) 179
MalI GATC 3 cut(s) 333, 599, 653
MboI GATC 3 cut(s) 331, 597, 651
MboII GAAGA 4 cut(s) 136, 367, 554, 578
MluCI AATT 1 cut(s) 292
Mph1103I ATGCAT 1 cut(s) 666
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 2 cut(s) 63, 423
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 2 cut(s) 80, 530
MspR9I CCNGG 1 cut(s) 531
MwoI GCNNNNNNNGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 531
NdeII GATC 3 cut(s) 331, 597, 651
NlaIII CATG 3 cut(s) 416, 491, 668
NlaIV GGNNCC 1 cut(s) 498
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 1 cut(s) 668
OliI CACNNNNGTG 1 cut(s) 38
PaeI GCATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 418
PkrI GCNGC 2 cut(s) 135, 628
PspN4I GGNNCC 1 cut(s) 498
PstNI CAGNNNCTG 1 cut(s) 10
PvuII CAGCTG 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 38
SaqAI TTAA 2 cut(s) 63, 423
SatI GCNGC 2 cut(s) 134, 627
Sau3AI GATC 3 cut(s) 331, 597, 651
ScrFI CCNGG 1 cut(s) 531
SfaNI GCATC 3 cut(s) 235, 281, 330
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SphI GCATGC 1 cut(s) 668
Sse9I AATT 1 cut(s) 292
SsiI CCGC 1 cut(s) 500
SspMI CTAG 3 cut(s) 99, 606, 669
StyD4I CCNGG 1 cut(s) 529
TaaI ACNGT 1 cut(s) 208
TaqI TCGA 1 cut(s) 450
TasI AATT 1 cut(s) 292
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 63, 423
Tru9I TTAA 2 cut(s) 63, 423
TscAI CASTG 2 cut(s) 40, 246
TseI GCWGC 2 cut(s) 133, 626
TspDTI ATGAA 3 cut(s) 44, 179, 602
TspRI CASTG 2 cut(s) 40, 246
Van91I CCANNNNNTGG 1 cut(s) 418
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 3 cut(s) 99, 606, 669
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.