MD08G1011300.v1.1

Protein of unknown function, DUF604

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009220
Physical Location & Seq
Forward (+)
871022 .. 871615
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1011300.v1.1.491

Sequence Viewer

Length: 594 bp
ATGCCCAGAACCTTAACCGTCGCCCACTTCAAAGCCCTCATGTTATTCCTCTATGTTTTCCTCAACCTCTACTTCCTCTTCCTCCACACGCTGTCAACCCTTCTCTCCCGCTACACTTCCTCCCCCACCACACGCAGCCACCTCGTCTTCGCCGTCATGTCCTCCTCCCGCTCCTGGTCCCACCGCGAGCCATACCTCCGCCTCTGGTGCTCCCCCGCCTCCATCCACGCCTTCGCGTTTCTTGATCGTGCACCAATCGACTCCTCCGGCGAGAAGTCCATCGCTCCTGTCGTCGTCTCCAGTGATACCTCCTACTTTCCGTACACTTTCAATAGAGGGCGCCGTTCCGCGATCCGCGTGGCGCGTATGGTCAAGGAGGTCGTCGATCGCGGCGAGCCGAATGTGAGGTGGTTCGTGTTTGGCGACGATGACACGGTGTTCTTCGTGGAGAATCTGGTGAGGACGATGTCAAAATACGATCACGATCGGTGGTTTTACGTCGGGAGCAATTCAGTTGCAGTCGCGGTCGCGGGGAGAGTGACGATGATTGAAGAAGAAGGCAATGGGGTTGGATTGGAGTGTGGCTGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

198

Amino Acids

22.56

Weight (kDa)

9.23

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 43 - 178 1.4e-07 Fringe-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 339
AccBSI CCGCTC 1 cut(s) 171
AccII CGCG 8 cut(s) 186, 236, 350, 357, 364, 390, 524, 530
AclWI GGATC 1 cut(s) 346
AcyI GRCGYC 1 cut(s) 340
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 3 cut(s) 31, 331, 551
AjnI CCWGG 1 cut(s) 173
Alw21I GWGCWC 2 cut(s) 212, 253
Alw26I GTCTC 1 cut(s) 301
Alw44I GTGCAC 1 cut(s) 249
AlwI GGATC 1 cut(s) 346
ApaLI GTGCAC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 135
AspLEI GCGC 2 cut(s) 342, 364
AspS9I GGNCC 1 cut(s) 177
AsuHPI GGTGA 1 cut(s) 469
AvaII GGWCC 1 cut(s) 177
BaeGI GKGCMC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 2 cut(s) 212, 253
BbvI GCAGC 1 cut(s) 147
BccI CCATC 2 cut(s) 230, 287
BceAI ACGGC 2 cut(s) 137, 327
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 301
BfoI RGCGCY 1 cut(s) 343
BisI GCNGC 2 cut(s) 136, 391
BlsI GCNGC 2 cut(s) 137, 392
Bme1390I CCNGG 1 cut(s) 175
Bme18I GGWCC 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 177
BmiI GGNNCC 2 cut(s) 179, 341
BmrFI CCNGG 1 cut(s) 175
BpiI GAAGAC 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 283
BsaBI GATNNNNATC 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 340
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 568
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 1 cut(s) 175
BseGI GGATG 1 cut(s) 222
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 568
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 2 cut(s) 154, 253
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 1 cut(s) 147
Bsh1236I CGCG 8 cut(s) 186, 236, 350, 357, 364, 390, 524, 530
Bsh1285I CGRYCG 3 cut(s) 388, 487, 528
BshNI GGYRCC 1 cut(s) 339
BsiEI CGRYCG 3 cut(s) 388, 487, 528
BsiHKAI GWGCWC 2 cut(s) 212, 253
BsiSI CCGG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 301
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 212, 253
Bsp143I GATC 5 cut(s) 244, 351, 385, 478, 484
BspFNI CGCG 8 cut(s) 186, 236, 350, 357, 364, 390, 524, 530
BspLI GGNNCC 2 cut(s) 179, 341
BspPI GGATC 1 cut(s) 346
BspT107I GGYRCC 1 cut(s) 339
BsrBI CCGCTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 568
BsrI ACTGG 1 cut(s) 300
BssMI GATC 5 cut(s) 244, 351, 385, 478, 484
BssNI GRCGYC 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 2 cut(s) 19, 436
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 340
BstC8I GCNNGC 3 cut(s) 188, 395, 587
BstF5I GGATG 1 cut(s) 222
BstFNI CGCG 8 cut(s) 186, 236, 350, 357, 364, 390, 524, 530
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 2 cut(s) 342, 364
BstKTI GATC 5 cut(s) 247, 354, 388, 481, 487
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 5 cut(s) 244, 351, 385, 478, 484
BstMCI CGRYCG 3 cut(s) 388, 487, 528
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 173
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 8 cut(s) 186, 236, 350, 357, 364, 390, 524, 530
BstV1I GCAGC 1 cut(s) 147
BstV2I GAAGAC 1 cut(s) 139
BtgZI GCGATG 1 cut(s) 265
BtsCI GGATG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 3 cut(s) 188, 395, 587
CfoI GCGC 2 cut(s) 342, 364
Cfr13I GGNCC 1 cut(s) 177
Csp6I GTAC 1 cut(s) 322
CviAII CATG 2 cut(s) 40, 157
CviJI RGCY 5 cut(s) 35, 138, 190, 397, 585
CviKI_1 RGCY 5 cut(s) 35, 138, 190, 397, 585
CviQI GTAC 1 cut(s) 322
DinI GGCGCC 1 cut(s) 341
DpnI GATC 5 cut(s) 246, 353, 387, 480, 486
DpnII GATC 5 cut(s) 244, 351, 385, 478, 484
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 173
EgeI GGCGCC 1 cut(s) 341
EheI GGCGCC 1 cut(s) 341
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 2 cut(s) 43, 160
FaiI YATR 5 cut(s) 41, 54, 158, 193, 368
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 39, 156
FauI CCCGC 4 cut(s) 116, 176, 223, 523
Fnu4HI GCNGC 2 cut(s) 136, 391
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 2 cut(s) 136, 391
GlaI GCGC 2 cut(s) 341, 363
GluI GCNGC 2 cut(s) 136, 391
GsuI CTGGAG 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 343
HapII CCGG 1 cut(s) 267
HhaI GCGC 2 cut(s) 342, 364
Hin1I GRCGYC 1 cut(s) 340
Hin1II CATG 2 cut(s) 43, 160
Hin6I GCGC 2 cut(s) 340, 362
HinP1I GCGC 2 cut(s) 340, 362
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HinfI GANTC 2 cut(s) 260, 451
HpaII CCGG 1 cut(s) 267
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 3 cut(s) 96, 251, 324
Hpy188III TCNNGA 3 cut(s) 242, 482, 502
Hpy8I GTNNAC 3 cut(s) 96, 251, 324
Hpy99I CGWCG 5 cut(s) 23, 296, 386, 428, 503
HpyAV CCTTC 3 cut(s) 110, 241, 551
HpyCH4III ACNGT 2 cut(s) 19, 436
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 2 cut(s) 251, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpySE526I ACGT 1 cut(s) 498
Hsp92I GRCGYC 1 cut(s) 340
Hsp92II CATG 2 cut(s) 43, 160
HspAI GCGC 2 cut(s) 340, 362
KasI GGCGCC 1 cut(s) 339
Kzo9I GATC 5 cut(s) 244, 351, 385, 478, 484
LmnI GCTCC 4 cut(s) 176, 215, 289, 504
LpnPI CCDG 9 cut(s) 19, 160, 187, 190, 280, 300, 313, 440, 571
Lsp1109I GCAGC 1 cut(s) 147
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 1 cut(s) 538
MalI GATC 5 cut(s) 246, 353, 387, 480, 486
MbiI CCGCTC 1 cut(s) 171
MboI GATC 5 cut(s) 244, 351, 385, 478, 484
MboII GAAGA 5 cut(s) 70, 139, 433, 563, 566
MhlI GDGCHC 2 cut(s) 212, 253
MluCI AATT 1 cut(s) 508
Mly113I GGCGCC 1 cut(s) 340
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 550
MseI TTAA 1 cut(s) 14
MspI CCGG 1 cut(s) 267
MspR9I CCNGG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 8 cut(s) 186, 236, 350, 357, 364, 390, 524, 530
MwoI GCNNNNNNNGC 1 cut(s) 207
NarI GGCGCC 1 cut(s) 340
NdeII GATC 5 cut(s) 244, 351, 385, 478, 484
NlaIII CATG 2 cut(s) 43, 160
NlaIV GGNNCC 2 cut(s) 179, 341
NmuCI GTSAC 1 cut(s) 538
PcsI WCGNNNNNNNCGW 4 cut(s) 150, 288, 390, 420
PfeI GAWTC 1 cut(s) 451
PflFI GACNNNGTC 1 cut(s) 466
PkrI GCNGC 2 cut(s) 137, 392
Ple19I CGATCG 2 cut(s) 388, 487
PleI GAGTC 1 cut(s) 254
PluTI GGCGCC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 173
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 2 cut(s) 179, 341
PspPI GGNCC 1 cut(s) 177
PsyI GACNNNGTC 1 cut(s) 466
PvuI CGATCG 2 cut(s) 388, 487
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 2 cut(s) 136, 391
Sau3AI GATC 5 cut(s) 244, 351, 385, 478, 484
Sau96I GGNCC 1 cut(s) 177
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 2 cut(s) 212, 253
SetI ASST 8 cut(s) 14, 69, 144, 198, 311, 381, 410, 501
SfoI GGCGCC 1 cut(s) 341
SinI GGWCC 1 cut(s) 177
Sse9I AATT 1 cut(s) 508
SspDI GGCGCC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 173
TaaI ACNGT 2 cut(s) 19, 436
TaiI ACGT 1 cut(s) 501
TaqI TCGA 2 cut(s) 258, 384
TasI AATT 1 cut(s) 508
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 1 cut(s) 451
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 1 cut(s) 135
Tsp45I GTSAC 1 cut(s) 538
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 466
VneI GTGCAC 1 cut(s) 249
VpaK11BI GGWCC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.