MD08G1035000.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011759
Physical Location & Seq
Reverse (-)
2451216 .. 2452265
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1035000.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCGATTACATTCCTTCATGCAGTATCACTCTTCGTTTTATCTGTTAACCTCGCAAATTGCCAATCATTTATCGGCGTAAACTACGGCCAAGTCGCCGACAACCTCCCGCCGCCGTCATCCACCGCGAATCTGCTCCAGTCCACCTCAATACAAAAGGTTCGGCTTTACGGTTCCGACCCGGCCATAATCAAAGCCCTGGCGAACACCGGCGTCGGAATCGTCATCGGAGCGGCGAACGGCGACATCCCGGGTCTCGCATCCGACCCGAGTTTCGCCAAGAGCTGGGTCGGCGCCAACGTGTCCCCGTTCTACCCGGCGAGCAACATTATCCTCATCACCGTCGGCAACGAGGTGTTGACATCCGGCGACCAGGGCCTAATTTCTCAGCTGGTCCCGGCGATTCAGAATGTCCAAAACGCCCTCAATTCCGCGTCTCTTGGAGGGAAAATCAAGGTCTCCACCGTACATTCCATGGCGGTGCTCAAGCAATCCGAACCGCCGTCCTCGGGAAGCTTCGACCCCGGTTTCGGCGACGTCATGAAGGGGTTGCTTGGGTTTAACAACGCCACCGGTTCGCCTTTCTCAATCAACCCGTACCCGTATTTCGCCTACCGGGGCGACCCGAGACCCGAGACACTTGCTTTTTGCCTATTCCAACCGAATGAGGGGCGGCTCGACACCAACACAAATATTAAGTACATGAACATGTTCGATGCTCAGGTGAAGTGGACGCAATAA

Protein Analysis

247

Amino Acids

25.99

Weight (kDa)

6.06

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 25 - 241 6e-44 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011759)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42720
fragaria_vesca FvH4_2g39880 FvH4_2g39880 FvH4_2g39880 FvH4_2g39880
malus_domestica MD08G1035000.v1.1 MD15G1031700.v1.1
prunus_persica Prupe.1G380300_v2.0.a1
pyrus_communis pycom08g02770 pycom15g02740
rosa_chinensis RchiOBHm_Chr6g0300921
rosa_laevigata RLG00000011290
rosa_multiflora Rmu_sc0006254.1_g000004
rosa_roxburghii Rroxscaffold_7G00167310
rosa_rugosa Rorug06G0299600
rosa_samantha Rh6AG413100 Rh6BG420300 Rh6CG427800 Rh6DG413100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 540
AccB1I GGYRCC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 285
AccBSI CCGCTC 1 cut(s) 233
AccII CGCG 2 cut(s) 128, 434
AciI CCGC 8 cut(s) 110, 113, 126, 233, 432, 479, 500, 673
AcoI YGGCCR 2 cut(s) 88, 183
AcyI GRCGYC 3 cut(s) 213, 294, 537
AfaI GTAC 3 cut(s) 468, 599, 701
AfiI CCNNNNNNNGG 4 cut(s) 285, 509, 530, 668
AflIII ACRYGT 2 cut(s) 300, 708
AgeI ACCGGT 1 cut(s) 572
AjnI CCWGG 2 cut(s) 198, 372
AluBI AGCT 3 cut(s) 285, 391, 516
AluI AGCT 3 cut(s) 285, 391, 516
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 5 cut(s) 260, 441, 463, 622, 629
Ama87I CYCGRG 5 cut(s) 250, 268, 508, 625, 632
AoxI GGCC 3 cut(s) 88, 183, 376
ArsI GACNNNNNNTTYG 4 cut(s) 257, 289, 512, 544
AsiGI ACCGGT 1 cut(s) 572
Asp700I GAANNNNTTC 1 cut(s) 710
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 2 cut(s) 376, 394
AsuC2I CCSGG 7 cut(s) 182, 251, 252, 317, 398, 525, 617
AsuHPI GGTGA 2 cut(s) 331, 736
AvaI CYCGRG 5 cut(s) 250, 268, 508, 625, 632
AvaII GGWCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 486
BceAI ACGGC 4 cut(s) 100, 103, 256, 487
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BciT130I CCWGG 2 cut(s) 200, 374
BcnI CCSGG 7 cut(s) 182, 251, 252, 317, 398, 525, 617
BcoDI GTCTC 5 cut(s) 260, 441, 463, 622, 629
BfoI RGCGCY 1 cut(s) 297
BisI GCNGC 3 cut(s) 113, 234, 674
BlsI GCNGC 3 cut(s) 114, 235, 675
Bme1390I CCNGG 9 cut(s) 182, 200, 251, 252, 317, 374, 398, 525, 617
Bme18I GGWCC 1 cut(s) 394
BmeT110I CYCGRG 5 cut(s) 250, 268, 508, 625, 632
BmgT120I GGNCC 2 cut(s) 376, 394
BmiI GGNNCC 3 cut(s) 175, 295, 396
BmrFI CCNGG 9 cut(s) 182, 200, 251, 252, 317, 374, 398, 525, 617
BmsI GCATC 2 cut(s) 269, 706
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 720
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 7 cut(s) 182, 251, 252, 317, 398, 525, 617
BsaHI GRCGYC 3 cut(s) 213, 294, 537
BsaI GGTCTC 3 cut(s) 260, 463, 622
BsaJI CCNNGG 7 cut(s) 198, 250, 373, 474, 507, 523, 616
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 509, 530, 668
Bse118I RCCGGY 2 cut(s) 209, 572
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 2 cut(s) 200, 374
BseDI CCNNGG 7 cut(s) 198, 250, 373, 474, 507, 523, 616
BseGI GGATG 4 cut(s) 119, 246, 260, 362
BseLI CCNNNNNNNGG 4 cut(s) 285, 509, 530, 668
BseMII CTCAG 2 cut(s) 401, 734
BseNI ACTGG 1 cut(s) 139
BseYI CCCAGC 1 cut(s) 285
Bsh1236I CGCG 2 cut(s) 128, 434
BshFI GGCC 3 cut(s) 90, 185, 378
BshNI GGYRCC 1 cut(s) 293
BshTI ACCGGT 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 486
BsiHKCI CYCGRG 5 cut(s) 250, 268, 508, 625, 632
BsiSI CCGG 9 cut(s) 182, 210, 251, 317, 366, 398, 525, 573, 616
BslFI GGGAC 2 cut(s) 289, 380
BslI CCNNNNNNNGG 4 cut(s) 285, 509, 530, 668
BsmAI GTCTC 5 cut(s) 260, 441, 463, 622, 629
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 2 cut(s) 289, 380
BsnI GGCC 3 cut(s) 90, 185, 378
Bso31I GGTCTC 3 cut(s) 260, 463, 622
BsoBI CYCGRG 5 cut(s) 250, 268, 508, 625, 632
Bsp1286I GDGCHC 1 cut(s) 486
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 8 cut(s) 110, 113, 126, 233, 432, 479, 500, 673
BspANI GGCC 3 cut(s) 90, 185, 378
BspCNI CTCAG 2 cut(s) 400, 733
BspFNI CGCG 2 cut(s) 128, 434
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 3 cut(s) 175, 295, 396
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 3 cut(s) 260, 463, 622
BsrBI CCGCTC 1 cut(s) 233
BsrFI RCCGGY 2 cut(s) 209, 572
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 2 cut(s) 209, 572
BssECI CCNNGG 7 cut(s) 198, 250, 373, 474, 507, 523, 616
BssNI GRCGYC 3 cut(s) 213, 294, 537
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 200, 374
Bst4CI ACNGT 3 cut(s) 173, 343, 466
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 3 cut(s) 213, 294, 537
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 2 cut(s) 387, 720
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 4 cut(s) 119, 246, 260, 362
BstFNI CGCG 2 cut(s) 128, 434
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstMAI GTCTC 5 cut(s) 260, 441, 463, 622, 629
BstMWI GCNNNNNNNGC 2 cut(s) 291, 375
BstNI CCWGG 2 cut(s) 200, 374
BstNSI RCATGY 1 cut(s) 712
BstSCI CCNGG 9 cut(s) 180, 198, 249, 250, 315, 372, 396, 523, 615
BstUI CGCG 2 cut(s) 128, 434
BsuRI GGCC 3 cut(s) 90, 185, 378
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 4 cut(s) 119, 246, 260, 362
Cac8I GCNNGC 1 cut(s) 322
CciI TCATGA 1 cut(s) 540
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 2 cut(s) 209, 572
Cfr13I GGNCC 2 cut(s) 376, 394
Cfr9I CCCGGG 1 cut(s) 250
CseI GACGC 2 cut(s) 202, 423
Csp6I GTAC 3 cut(s) 467, 598, 700
CspAI ACCGGT 1 cut(s) 572
CviAII CATG 5 cut(s) 20, 475, 541, 703, 709
CviJI RGCY 9 cut(s) 90, 166, 185, 197, 285, 378, 391, 516, 676
CviKI_1 RGCY 9 cut(s) 90, 166, 185, 197, 285, 378, 391, 516, 676
CviQI GTAC 3 cut(s) 467, 598, 700
DdeI CTNAG 2 cut(s) 387, 720
DinI GGCGCC 1 cut(s) 295
EaeI YGGCCR 2 cut(s) 88, 183
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 474
Eco31I GGTCTC 3 cut(s) 260, 463, 622
Eco47I GGWCC 1 cut(s) 394
Eco88I CYCGRG 5 cut(s) 250, 268, 508, 625, 632
EcoO109I RGGNCCY 1 cut(s) 376
EcoRII CCWGG 2 cut(s) 198, 372
EcoT14I CCWWGG 1 cut(s) 474
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 5 cut(s) 23, 478, 544, 706, 712
FaiI YATR 6 cut(s) 21, 188, 476, 542, 704, 710
FaqI GGGAC 2 cut(s) 289, 380
FatI CATG 5 cut(s) 19, 474, 540, 702, 708
FauI CCCGC 1 cut(s) 117
Fnu4HI GCNGC 3 cut(s) 113, 234, 674
FokI GGATG 4 cut(s) 106, 233, 247, 349
Fsp4HI GCNGC 3 cut(s) 113, 234, 674
GlaI GCGC 1 cut(s) 295
GluI GCNGC 3 cut(s) 113, 234, 674
GsaI CCCAGC 1 cut(s) 289
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 3 cut(s) 90, 185, 378
HapII CCGG 9 cut(s) 182, 210, 251, 317, 366, 398, 525, 573, 616
HgaI GACGC 2 cut(s) 202, 423
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 3 cut(s) 213, 294, 537
Hin1II CATG 5 cut(s) 23, 478, 544, 706, 712
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 2 cut(s) 49, 360
HindII GTYRAC 2 cut(s) 49, 360
HindIII AAGCTT 1 cut(s) 514
HinfI GANTC 3 cut(s) 130, 219, 403
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 9 cut(s) 182, 210, 251, 317, 366, 398, 525, 573, 616
HphI GGTGA 2 cut(s) 331, 736
Hpy166II GTNNAC 5 cut(s) 49, 82, 144, 360, 732
Hpy188I TCNGA 6 cut(s) 178, 218, 230, 265, 408, 496
Hpy188III TCNNGA 2 cut(s) 510, 541
Hpy8I GTNNAC 5 cut(s) 49, 82, 144, 360, 732
Hpy99I CGWCG 3 cut(s) 218, 347, 539
HpyAV CCTTC 2 cut(s) 26, 538
HpyCH4III ACNGT 3 cut(s) 173, 343, 466
HpyCH4IV ACGT 2 cut(s) 300, 537
HpyCH4V TGCA 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 2 cut(s) 291, 375
HpyF3I CTNAG 2 cut(s) 387, 720
HpySE526I ACGT 2 cut(s) 300, 537
Hsp92I GRCGYC 3 cut(s) 213, 294, 537
Hsp92II CATG 5 cut(s) 23, 478, 544, 706, 712
HspAI GCGC 1 cut(s) 294
KasI GGCGCC 1 cut(s) 293
KspAI GTTAAC 1 cut(s) 49
LmnI GCTCC 2 cut(s) 141, 230
LweI GCATC 2 cut(s) 269, 706
MaeII ACGT 2 cut(s) 300, 537
MbiI CCGCTC 1 cut(s) 233
MboII GAAGA 1 cut(s) 25
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 3 cut(s) 58, 381, 427
Mly113I GGCGCC 1 cut(s) 294
MmeI TCCRAC 4 cut(s) 196, 201, 288, 682
MnlI CCTC 9 cut(s) 62, 116, 157, 344, 346, 434, 437, 517, 661
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 3 cut(s) 48, 561, 696
MslI CAYNNNNRTG 2 cut(s) 479, 707
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 9 cut(s) 182, 210, 251, 317, 366, 398, 525, 573, 616
MspR9I CCNGG 9 cut(s) 182, 200, 251, 252, 317, 374, 398, 525, 617
MvaI CCWGG 2 cut(s) 200, 374
MvnI CGCG 2 cut(s) 128, 434
MwoI GCNNNNNNNGC 2 cut(s) 291, 375
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 7 cut(s) 182, 251, 252, 317, 398, 525, 617
NcoI CCATGG 1 cut(s) 474
NlaIII CATG 5 cut(s) 23, 478, 544, 706, 712
NlaIV GGNNCC 3 cut(s) 175, 295, 396
NspI RCATGY 1 cut(s) 712
PagI TCATGA 1 cut(s) 540
PciI ACATGT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 3 cut(s) 130, 219, 403
PflMI CCANNNNNTGG 1 cut(s) 285
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 3 cut(s) 114, 235, 675
PluTI GGCGCC 1 cut(s) 297
PscI ACATGT 1 cut(s) 708
Psp6I CCWGG 2 cut(s) 198, 372
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 2 cut(s) 198, 372
PspN4I GGNNCC 3 cut(s) 175, 295, 396
PspPI GGNCC 2 cut(s) 376, 394
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 3 cut(s) 468, 599, 701
RsaNI GTAC 3 cut(s) 467, 598, 700
RseI CAYNNNNRTG 2 cut(s) 479, 707
SaqAI TTAA 3 cut(s) 48, 561, 696
SatI GCNGC 3 cut(s) 113, 234, 674
Sau96I GGNCC 2 cut(s) 376, 394
ScrFI CCNGG 9 cut(s) 182, 200, 251, 252, 317, 374, 398, 525, 617
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 2 cut(s) 269, 706
SfoI GGCGCC 1 cut(s) 295
SgrAI CRCCGGYG 1 cut(s) 209
SinI GGWCC 1 cut(s) 394
SmaI CCCGGG 1 cut(s) 252
SmiMI CAYNNNNRTG 2 cut(s) 479, 707
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 3 cut(s) 58, 381, 427
SsiI CCGC 8 cut(s) 110, 113, 126, 233, 432, 479, 500, 673
SspDI GGCGCC 1 cut(s) 293
SspI AATATT 1 cut(s) 694
StyD4I CCNGG 9 cut(s) 180, 198, 249, 250, 315, 372, 396, 523, 615
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 3 cut(s) 173, 343, 466
TaiI ACGT 2 cut(s) 303, 540
TaqI TCGA 3 cut(s) 519, 678, 714
TasI AATT 3 cut(s) 58, 381, 427
TatI WGTACW 1 cut(s) 699
TauI GCSGC 3 cut(s) 115, 236, 676
TfiI GAWTC 3 cut(s) 130, 219, 403
Tru1I TTAA 3 cut(s) 48, 561, 696
Tru9I TTAA 3 cut(s) 48, 561, 696
TspDTI ATGAA 3 cut(s) 8, 557, 719
TspMI CCCGGG 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 394
XceI RCATGY 1 cut(s) 712
XmaI CCCGGG 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 710
ZraI GACGTC 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.