Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0014895

Physical Location & Seq

Genomic Coordinates Forward (+)

4663510 .. 4665275

Gene Structure 1766 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1058500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 822 bp

ATGTCGATTTTACCGAAGGGGGATTCGATTGAAATCCGAGAGGTGTGGAACGATAATCTCGATGAAGAGTTTGCTCTGATTCGTGATATTGTTTTGAAGTATCCGTATGTGGCTATGGATACTGAGTTTCCGGGCGTGGTTCTTCGCCCGGTGGGGGATTTTAAGAACATCAATGAGTTTAACTACAAGACTCTGAAGGACAATGTTGATATGTTGAAATTGATTCAGTTGGGTCTCACTTTCTTTGATGAGAACGGCAACCTTCCCACTCTGTCGACCGAGAACCAGTACATTTGGCAATTCAACTTTCGCGAGTTCAACGTGAGCGAAGACATATACGCGAGCGATTCAATTGAGTTGTTGCGTCAGTGTGGGATCGATTTCAAGAAGAACAATGAGAAGGGGATCGATGTGAATCGGTTTGGCGAGCTTTTGATGGCTTCAGGGCTTGTATTGAGCGACAATGTGTATTGGGTTACGTTCCATAGTGGATACGACTTTGGATACCTGCTTAAGTTGTTGACTTGTCGGAGTTTGCCTGACGCGCAGACGGAGTTCTTTAATCTGATCAAGATTTACTTCCCAGTGTTGTATGATATCAAGCATCTGATGAAGTTCTGCAACAGCCTTCACGGTGGCTTAAACAAGCTTGCAGAACTGTTGGAAGTGGAACGAGTTGGTGTGTGCCATCAAGCAGGCTCCGACAGTCTGCTTACAGCGTGTACGTTTAGGAAGTTGAGGGATACTTTCTTCAGTGGCTCAGCGGAGAAGTATTCCGGTGTGTTGTATGGTCTCGGCGTTGAGAATGGACAGAGTAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000288 GO:0000291 GO:0000932 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004535 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0017148 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1990904 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

CAF1

Protein Analysis

274

Amino Acids

31.24

Weight (kDa)

4.83

Isoelectric Point (pI)

32.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1	PF04857	15 - 136	4.6e-13	CAF1 family ribonuclease
CAF1	PF04857	158 - 243	6.7e-06	CAF1 family ribonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10960
fragaria_vesca	FvH4_2g40930
malus_domestica	MD08G1058500.v1.1 MD15G1038000.v1.1
prunus_persica	Prupe.1G391900_v2.0.a1
pyrus_communis	pycom08g04840 pycom15g03520
rosa_chinensis	RchiOBHm_Chr6g0299631
rosa_laevigata	RLG00000011401
rosa_roxburghii	Rroxscaffold_7G00168490
rosa_rugosa	Rorug06G0291200
rosa_samantha	Rh6AG402000 Rh6BG410400 Rh6CG416100 Rh6DG403300
rosa_wichuraiana	Rw6G035180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	516
AccI	GTMKAC	1 cut(s)	275
AccII	CGCG	3 cut(s)	312, 341, 545
AciI	CCGC	1 cut(s)	764
AclWI	GGATC	2 cut(s)	383, 413
AcuI	CTGAAG	3 cut(s)	215, 426, 736
AfaI	GTAC	2 cut(s)	290, 724
AfiI	CCNNNNNNNGG	1 cut(s)	154
AflII	CTTAAG	1 cut(s)	512
AgsI	TTSAA	7 cut(s)	32, 97, 217, 304, 319, 351, 385
AluBI	AGCT	2 cut(s)	430, 649
AluI	AGCT	2 cut(s)	430, 649
Alw26I	GTCTC	2 cut(s)	239, 797
AlwI	GGATC	2 cut(s)	383, 413
AspLEI	GCGC	1 cut(s)	547
AsuC2I	CCSGG	2 cut(s)	132, 149
BbsI	GAAGAC	1 cut(s)	336
BccI	CCATC	2 cut(s)	430, 696
BceAI	ACGGC	1 cut(s)	271
BciVI	GTATCC	5 cut(s)	111, 112, 485, 497, 736
BclI	TGATCA	1 cut(s)	567
BcnI	CCSGG	2 cut(s)	132, 149
BcoDI	GTCTC	2 cut(s)	239, 797
BfrI	CTTAAG	1 cut(s)	512
BfuAI	ACCTGC	1 cut(s)	516
BfuI	GTATCC	5 cut(s)	111, 112, 485, 497, 736
BlpI	GCTNAGC	1 cut(s)	760
Bme1390I	CCNGG	2 cut(s)	132, 149
BmiI	GGNNCC	1 cut(s)	700
BmrFI	CCNGG	2 cut(s)	132, 149
BmrI	ACTGGG	1 cut(s)	578
BmsI	GCATC	1 cut(s)	613
BmuI	ACTGGG	1 cut(s)	578
BpiI	GAAGAC	1 cut(s)	336
Bpu1102I	GCTNAGC	1 cut(s)	760
BpuMI	CCSGG	2 cut(s)	132, 149
Bsa29I	ATCGAT	2 cut(s)	378, 408
BsaBI	GATNNNNATC	2 cut(s)	32, 414
BsaI	GGTCTC	2 cut(s)	239, 797
BsaWI	WCCGGW	1 cut(s)	776
Bsc4I	CCNNNNNNNGG	1 cut(s)	154
Bse1I	ACTGG	2 cut(s)	286, 584
Bse8I	GATNNNNATC	2 cut(s)	32, 414
BseCI	ATCGAT	2 cut(s)	378, 408
BseJI	GATNNNNATC	2 cut(s)	32, 414
BseLI	CCNNNNNNNGG	1 cut(s)	154
BseMII	CTCAG	2 cut(s)	114, 774
BseNI	ACTGG	2 cut(s)	286, 584
Bsh1236I	CGCG	3 cut(s)	312, 341, 545
Bsh1285I	CGRYCG	1 cut(s)	279
BshVI	ATCGAT	2 cut(s)	378, 408
BsiEI	CGRYCG	1 cut(s)	279
BsiSI	CCGG	3 cut(s)	131, 149, 777
BslI	CCNNNNNNNGG	1 cut(s)	154
BsmAI	GTCTC	2 cut(s)	239, 797
Bso31I	GGTCTC	2 cut(s)	239, 797
Bsp143I	GATC	3 cut(s)	375, 405, 567
Bsp1720I	GCTNAGC	1 cut(s)	760
Bsp68I	TCGCGA	1 cut(s)	312
BspACI	CCGC	1 cut(s)	764
BspCNI	CTCAG	2 cut(s)	115, 773
BspDI	ATCGAT	2 cut(s)	378, 408
BspFNI	CGCG	3 cut(s)	312, 341, 545
BspLI	GGNNCC	1 cut(s)	700
BspMI	ACCTGC	1 cut(s)	516
BspPI	GGATC	2 cut(s)	383, 413
BspTI	CTTAAG	1 cut(s)	512
BspTNI	GGTCTC	2 cut(s)	239, 797
BsrI	ACTGG	2 cut(s)	286, 584
BssMI	GATC	3 cut(s)	375, 405, 567
Bst4CI	ACNGT	3 cut(s)	635, 660, 707
Bst6I	CTCTTC	1 cut(s)	60
BstAFI	CTTAAG	1 cut(s)	512
BstC8I	GCNNGC	4 cut(s)	343, 428, 651, 697
BstDEI	CTNAG	2 cut(s)	123, 760
BstFNI	CGCG	3 cut(s)	312, 341, 545
BstHHI	GCGC	1 cut(s)	547
BstKTI	GATC	3 cut(s)	378, 408, 570
BstMAI	GTCTC	2 cut(s)	239, 797
BstMBI	GATC	3 cut(s)	375, 405, 567
BstMCI	CGRYCG	1 cut(s)	279
BstMWI	GCNNNNNNNGC	1 cut(s)	544
BstSCI	CCNGG	2 cut(s)	130, 147
BstUI	CGCG	3 cut(s)	312, 341, 545
BstV2I	GAAGAC	1 cut(s)	336
Bsu15I	ATCGAT	2 cut(s)	378, 408
BsuI	GTATCC	5 cut(s)	111, 112, 485, 497, 736
BsuTUI	ATCGAT	2 cut(s)	378, 408
BtsIMutI	CAGTG	3 cut(s)	374, 591, 760
BtuMI	TCGCGA	1 cut(s)	312
BveI	ACCTGC	1 cut(s)	516
Cac8I	GCNNGC	4 cut(s)	343, 428, 651, 697
CfoI	GCGC	1 cut(s)	547
ClaI	ATCGAT	2 cut(s)	378, 408
CseI	GACGC	2 cut(s)	353, 551
Csp6I	GTAC	2 cut(s)	289, 723
CviJI	RGCY	9 cut(s)	113, 430, 440, 448, 627, 639, 649, 699, 759
CviKI_1	RGCY	9 cut(s)	113, 430, 440, 448, 627, 639, 649, 699, 759
CviQI	GTAC	2 cut(s)	289, 723
DdeI	CTNAG	2 cut(s)	123, 760
DpnI	GATC	3 cut(s)	377, 407, 569
DpnII	GATC	3 cut(s)	375, 405, 567
Eam1104I	CTCTTC	1 cut(s)	60
EarI	CTCTTC	1 cut(s)	60
Eco31I	GGTCTC	2 cut(s)	239, 797
Eco32I	GATATC	1 cut(s)	598
Eco57I	CTGAAG	3 cut(s)	215, 426, 736
EcoRV	GATATC	1 cut(s)	598
FaiI	YATR	8 cut(s)	108, 116, 212, 335, 337, 486, 594, 789
FalI	AAGNNNNNCTT	2 cut(s)	563, 595
FbaI	TGATCA	1 cut(s)	567
FblI	GTMKAC	1 cut(s)	275
GlaI	GCGC	1 cut(s)	546
HapII	CCGG	3 cut(s)	131, 149, 777
HgaI	GACGC	2 cut(s)	353, 551
HhaI	GCGC	1 cut(s)	547
Hin6I	GCGC	1 cut(s)	545
HinP1I	GCGC	1 cut(s)	545
HincII	GTYRAC	2 cut(s)	276, 522
HindII	GTYRAC	2 cut(s)	276, 522
HindIII	AAGCTT	1 cut(s)	647
HinfI	GANTC	6 cut(s)	23, 79, 190, 223, 347, 415
HpaII	CCGG	3 cut(s)	131, 149, 777
Hpy166II	GTNNAC	3 cut(s)	276, 522, 723
Hpy188I	TCNGA	7 cut(s)	38, 78, 195, 531, 567, 609, 703
Hpy188III	TCNNGA	5 cut(s)	59, 83, 311, 385, 571
Hpy8I	GTNNAC	3 cut(s)	276, 522, 723
HpyAV	CCTTC	5 cut(s)	10, 190, 272, 394, 638
HpyCH4III	ACNGT	3 cut(s)	635, 660, 707
HpyCH4IV	ACGT	3 cut(s)	321, 479, 725
HpyCH4V	TGCA	2 cut(s)	621, 653
HpyF10VI	GCNNNNNNNGC	1 cut(s)	544
HpyF3I	CTNAG	2 cut(s)	123, 760
HpySE526I	ACGT	3 cut(s)	321, 479, 725
HspAI	GCGC	1 cut(s)	545
Ksp22I	TGATCA	1 cut(s)	567
Kzo9I	GATC	3 cut(s)	375, 405, 567
LmnI	GCTCC	1 cut(s)	704
LpnPI	CCDG	9 cut(s)	144, 162, 299, 429, 521, 552, 597, 681, 790
LweI	GCATC	1 cut(s)	613
MaeII	ACGT	3 cut(s)	321, 479, 725
MaeIII	GTNAC	2 cut(s)	475, 815
MalI	GATC	3 cut(s)	377, 407, 569
MboI	GATC	3 cut(s)	375, 405, 567
MboII	GAAGA	5 cut(s)	77, 134, 341, 400, 742
MfeI	CAATTG	1 cut(s)	351
MluCI	AATT	3 cut(s)	218, 299, 351
MlyI	GAGTC	1 cut(s)	184
MmeI	TCCRAC	3 cut(s)	509, 642, 726
MnlI	CCTC	2 cut(s)	34, 732
MseI	TTAA	5 cut(s)	162, 180, 513, 561, 641
MspA1I	CMGCKG	1 cut(s)	764
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	3 cut(s)	131, 149, 777
MspR9I	CCNGG	2 cut(s)	132, 149
MunI	CAATTG	1 cut(s)	351
MvnI	CGCG	3 cut(s)	312, 341, 545
MwoI	GCNNNNNNNGC	1 cut(s)	544
NciI	CCSGG	2 cut(s)	132, 149
NdeII	GATC	3 cut(s)	375, 405, 567
NlaIV	GGNNCC	1 cut(s)	700
NmeAIII	GCCGAG	1 cut(s)	774
NruI	TCGCGA	1 cut(s)	312
PcsI	WCGNNNNNNNCGW	2 cut(s)	11, 57
PfeI	GAWTC	5 cut(s)	23, 79, 223, 347, 415
PleI	GAGTC	1 cut(s)	184
PpsI	GAGTC	1 cut(s)	184
PspN4I	GGNNCC	1 cut(s)	700
RruI	TCGCGA	1 cut(s)	312
RsaI	GTAC	2 cut(s)	290, 724
RsaNI	GTAC	2 cut(s)	289, 723
SalI	GTCGAC	1 cut(s)	274
SaqAI	TTAA	5 cut(s)	162, 180, 513, 561, 641
Sau3AI	GATC	3 cut(s)	375, 405, 567
SchI	GAGTC	1 cut(s)	184
ScrFI	CCNGG	2 cut(s)	132, 149
SetI	ASST	8 cut(s)	45, 264, 324, 432, 482, 510, 651, 728
SfaNI	GCATC	1 cut(s)	613
SmlI	CTYRAG	1 cut(s)	512
SmoI	CTYRAG	1 cut(s)	512
Sse9I	AATT	3 cut(s)	218, 299, 351
SsiI	CCGC	1 cut(s)	764
StyD4I	CCNGG	2 cut(s)	130, 147
TaaI	ACNGT	3 cut(s)	635, 660, 707
TaiI	ACGT	3 cut(s)	324, 482, 728
TaqI	TCGA	6 cut(s)	5, 26, 60, 275, 378, 408
TaqII	GACCGA	1 cut(s)	293
TasI	AATT	3 cut(s)	218, 299, 351
TatI	WGTACW	1 cut(s)	288
TfiI	GAWTC	5 cut(s)	23, 79, 223, 347, 415
Tru1I	TTAA	5 cut(s)	162, 180, 513, 561, 641
Tru9I	TTAA	5 cut(s)	162, 180, 513, 561, 641
TscAI	CASTG	3 cut(s)	374, 591, 760
TspDTI	ATGAA	2 cut(s)	78, 626
TspGWI	ACGGA	2 cut(s)	93, 566
TspRI	CASTG	3 cut(s)	374, 591, 760
Vha464I	CTTAAG	1 cut(s)	512
XmiI	GTMKAC	1 cut(s)	275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.