MD08G1072800.v1.1

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008332
Physical Location & Seq
Reverse (-)
5889158 .. 5892532
3375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1072800.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGGTAATGTTTGTTAGAAGAAGAGGCGTCGTCGCCGTGGTTGTGGCATGCCTCGCTATCTTCCAAGGACTTGTTCTTAGCGTTCATGGCACTGGTTTCAATTATAAGGACGCCCTCACCAAGTCCATTATTTTCCTTGAGGCTCAAAGATCAGGGAAACTCCCTCCCAATCATAGACCTGCATGGAGAGGCGACTCCGCCCTCGATGACGGCAAAGAAGCAAATGTCGACCTTGCTGGAGGATACTATGATGCCGGGGACAACGTCAAGTACGGTCTTCCGATGGCATTCACCGTCACAACTCTGTCGTGGGCCGCCATGTTTTACAGGCAACAGCTCCAAGCTACTGGAGAACTGGAAAACGTTCTTGCCGGCATCAAATGGGGCACCGATTATTTTCTCAAAGCCGCCAGCAAAAACAGATTATACGTTCAGGTTGGAGACCCAAATAAAGACCATGAATGTTGGACTAGACCAGAAGATATGCAAACACCAAGAACTGTGTTGAAGATTGATGATAAAACACCTGGAACTGAAATTGCAGCTGAAACTTCAGCCGCCATGGCTGCCTCTGCAATTGTGTTCAGAAAAACAGATCGTGCCTATGCTCGTAGGCTACTCAACAAAGCTAAACTCCTCTTTCAAATGGCTAAGGCGCATAAGGGAACTTACGATGGAGAGTGCCCTTTCTACTGCTCTTACTCTGGCTATAATGATGAGCTATTGTGGGCAGCAACATGGTTGTACATTGCCACAAAGAGGCCCGTGTATCTCAAGTACATTCAGGAAGAAGCCATAAGCGCCACCGTGTCTGAGTTTAGCTGGGACCTTAAATACGCCGGAGCACAAGTCCTCCTCGCCAAGCTGTTCTTTGATGGTGAAAAATCCCTAGAAAACTACAAAAACGGTGCTGACAGTTACATTTGCTCAAACATCCCCGACAGCCCTTACCACCAAATCACAATGACACCGGGCGGTATGATCCACTTGAGAGACGGAGCCAACACCCAATACGTCACCGGAACAGCCCACTTATTCGCCGTCTACAGCGACATCCTCGCTCAACACAACCAAAAAGTCAACTGCGGAGGCAAACAGTTCGACTCTGCCGCCCTCATGGCCTTTGCCAAGAAGCAAATGGACTACTTGTTGGGTGATAATCCCAAAAAAAGATCCTACATGGTCGGGTTTGGACCCAACGCGCCCCAACAGCCCCACCACAGAGGAGCTTCGGTTCCGAAGTCGGAAAACAGCCTCGTTAACTGCGCCATGAGCTTCGTGTACTGGTTCAAGAAGGATGTGCCAAACCCTAATGAGTTGACTGGTGCCATCGTCGGTGGGCCCGATAGGTTTGATAACTTCGAGGACAAACGTTGGGCGTCGTCGTATACCGAGCCATGCACTTACGTGAACTCGCTCGCGGTCGGACCGCTGGCGAAGCTTGCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

484

Amino Acids

53.59

Weight (kDa)

8.54

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 35 - 476 2.2e-152 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 105
Acc36I ACCTGC 1 cut(s) 187
AccB1I GGYRCC 2 cut(s) 386, 1325
AccI GTMKAC 3 cut(s) 228, 1044, 1388
AccII CGCG 2 cut(s) 1202, 1421
AclI AACGTT 2 cut(s) 363, 1372
AclWI GGATC 2 cut(s) 976, 1167
AcuI CTGAAG 1 cut(s) 537
AcyI GRCGYC 3 cut(s) 27, 111, 1379
AfaI GTAC 4 cut(s) 272, 746, 779, 1283
AfiI CCNNNNNNNGG 1 cut(s) 759
AgsI TTSAA 4 cut(s) 100, 508, 644, 1291
AjnI CCWGG 1 cut(s) 526
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AleI CACNNNNGTG 1 cut(s) 1406
AloI GAACNNNNNNTCC 2 cut(s) 1218, 1250
Alw21I GWGCWC 1 cut(s) 847
Alw26I GTCTC 2 cut(s) 435, 987
AlwI GGATC 2 cut(s) 976, 1167
AoxI GGCC 5 cut(s) 312, 761, 1119, 1340, 1446
ApaI GGGCCC 1 cut(s) 1344
ApeKI GCWGC 3 cut(s) 542, 566, 731
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 4 cut(s) 658, 803, 1204, 1268
AspS9I GGNCC 7 cut(s) 312, 762, 826, 1193, 1340, 1341, 1427
AsuC2I CCSGG 2 cut(s) 256, 972
AsuHPI GGTGA 5 cut(s) 109, 283, 890, 1009, 1166
AvaII GGWCC 3 cut(s) 826, 1193, 1427
BaeGI GKGCMC 3 cut(s) 389, 686, 1344
BanI GGYRCC 2 cut(s) 386, 1325
BanII GRGCYC 1 cut(s) 1344
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 847
BbvI GCAGC 3 cut(s) 553, 554, 743
BccI CCATC 4 cut(s) 277, 668, 869, 1337
BceAI ACGGC 3 cut(s) 20, 226, 1025
BcgI CGANNNNNNTGC 2 cut(s) 1081, 1115
BciT130I CCWGG 1 cut(s) 528
BciVI GTATCC 1 cut(s) 236
BcnI CCSGG 2 cut(s) 256, 972
BcoDI GTCTC 2 cut(s) 435, 987
BfaI CTAG 2 cut(s) 471, 890
BfmI CTRYAG 1 cut(s) 1045
BfoI RGCGCY 1 cut(s) 804
BfuAI ACCTGC 1 cut(s) 187
BfuI GTATCC 1 cut(s) 236
BglI GCCNNNNNGGC 2 cut(s) 563, 1118
BisI GCNGC 8 cut(s) 315, 408, 543, 558, 567, 732, 1110, 1446
BlsI GCNGC 8 cut(s) 316, 409, 544, 559, 568, 733, 1111, 1447
Bme1390I CCNGG 3 cut(s) 256, 528, 972
Bme18I GGWCC 3 cut(s) 826, 1193, 1427
BmgT120I GGNCC 7 cut(s) 312, 762, 826, 1193, 1340, 1341, 1427
BmiI GGNNCC 7 cut(s) 388, 827, 1000, 1195, 1236, 1327, 1342
BmrFI CCNGG 3 cut(s) 256, 528, 972
BmsI GCATC 2 cut(s) 241, 384
BpiI GAAGAC 1 cut(s) 269
BpmI CTGGAG 2 cut(s) 258, 369
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 3 cut(s) 158, 758, 1009
BpuMI CCSGG 2 cut(s) 256, 972
BsaAI YACGTR 1 cut(s) 1408
BsaHI GRCGYC 3 cut(s) 27, 111, 1379
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 4 cut(s) 36, 64, 255, 561
BsaWI WCCGGW 1 cut(s) 1019
BsaXI ACNNNNNCTCC 6 cut(s) 834, 837, 864, 867, 1218, 1248
Bsc4I CCNNNNNNNGG 1 cut(s) 759
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 5 cut(s) 97, 352, 360, 1289, 1327
Bse3DI GCAATG 1 cut(s) 747
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 4 cut(s) 36, 64, 255, 561
BseGI GGATG 3 cut(s) 933, 1053, 1303
BseLI CCNNNNNNNGG 1 cut(s) 759
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 5 cut(s) 97, 352, 360, 1289, 1327
BseRI GAGGAG 3 cut(s) 626, 845, 1239
BseSI GKGCMC 3 cut(s) 389, 686, 1344
BseXI GCAGC 3 cut(s) 553, 554, 743
BseYI CCCAGC 1 cut(s) 822
Bsh1236I CGCG 2 cut(s) 1202, 1421
Bsh1285I CGRYCG 1 cut(s) 1425
BshFI GGCC 5 cut(s) 314, 763, 1121, 1342, 1448
BshNI GGYRCC 2 cut(s) 386, 1325
BsiEI CGRYCG 1 cut(s) 1425
BsiHKAI GWGCWC 1 cut(s) 847
BsiSI CCGG 5 cut(s) 255, 372, 840, 971, 1020
BslFI GGGAC 2 cut(s) 272, 839
BslI CCNNNNNNNGG 1 cut(s) 759
BsmAI GTCTC 2 cut(s) 435, 987
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 2 cut(s) 272, 839
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 5 cut(s) 314, 763, 1121, 1342, 1448
Bso31I GGTCTC 1 cut(s) 435
Bsp120I GGGCCC 1 cut(s) 1340
Bsp1286I GDGCHC 4 cut(s) 389, 686, 847, 1344
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 4 cut(s) 149, 595, 981, 1172
Bsp19I CCATGG 1 cut(s) 561
BspANI GGCC 5 cut(s) 314, 763, 1121, 1342, 1448
BspCNI CTCAG 1 cut(s) 805
BspFNI CGCG 2 cut(s) 1202, 1421
BspLI GGNNCC 7 cut(s) 388, 827, 1000, 1195, 1236, 1327, 1342
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 2 cut(s) 976, 1167
BspT107I GGYRCC 2 cut(s) 386, 1325
BspTNI GGTCTC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 747
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 5 cut(s) 97, 352, 360, 1289, 1327
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 4 cut(s) 36, 64, 255, 561
BssMI GATC 4 cut(s) 149, 595, 981, 1172
BssNAI GTATAC 1 cut(s) 1389
BssNI GRCGYC 3 cut(s) 27, 111, 1379
BssT1I CCWWGG 2 cut(s) 64, 561
Bst1107I GTATAC 1 cut(s) 1389
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 7 cut(s) 275, 295, 502, 808, 908, 917, 1098
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 3 cut(s) 27, 111, 1379
BstAUI TGTACA 1 cut(s) 744
BstBAI YACGTR 1 cut(s) 1408
BstC8I GCNNGC 6 cut(s) 49, 373, 412, 1419, 1434, 1443
BstDEI CTNAG 3 cut(s) 77, 651, 813
BstDSI CCRYGG 2 cut(s) 36, 561
BstF5I GGATG 3 cut(s) 933, 1053, 1303
BstFNI CGCG 2 cut(s) 1202, 1421
BstH2I RGCGCY 1 cut(s) 804
BstHHI GCGC 4 cut(s) 658, 803, 1204, 1268
BstKTI GATC 4 cut(s) 152, 598, 984, 1175
BstMAI GTCTC 2 cut(s) 435, 987
BstMBI GATC 4 cut(s) 149, 595, 981, 1172
BstMCI CGRYCG 1 cut(s) 1425
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 3 cut(s) 254, 526, 970
BstSFI CTRYAG 1 cut(s) 1045
BstSLI GKGCMC 3 cut(s) 389, 686, 1344
BstUI CGCG 2 cut(s) 1202, 1421
BstV1I GCAGC 3 cut(s) 553, 554, 743
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 1172
BstXI CCANNNNNNTGG 1 cut(s) 347
BstYI RGATCY 1 cut(s) 1172
BstZ17I GTATAC 1 cut(s) 1389
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 5 cut(s) 314, 763, 1121, 1342, 1448
BtgI CCRYGG 2 cut(s) 36, 561
BtsCI GGATG 3 cut(s) 933, 1053, 1303
BtsIMutI CAGTG 1 cut(s) 90
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 6 cut(s) 49, 373, 412, 1419, 1434, 1443
CfoI GCGC 4 cut(s) 658, 803, 1204, 1268
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 7 cut(s) 312, 762, 826, 1193, 1340, 1341, 1427
CpoI CGGWCCG 1 cut(s) 1427
CseI GACGC 3 cut(s) 16, 119, 1368
Csp6I GTAC 4 cut(s) 271, 745, 778, 1282
CspI CGGWCCG 1 cut(s) 1427
CviQI GTAC 4 cut(s) 271, 745, 778, 1282
DdeI CTNAG 3 cut(s) 77, 651, 813
DpnI GATC 4 cut(s) 151, 597, 983, 1174
DpnII GATC 4 cut(s) 149, 595, 981, 1172
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 64, 561
Eco24I GRGCYC 1 cut(s) 1344
Eco31I GGTCTC 1 cut(s) 435
Eco47I GGWCC 3 cut(s) 826, 1193, 1427
Eco57I CTGAAG 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 526
EcoT14I CCWWGG 2 cut(s) 64, 561
EcoT38I GRGCYC 1 cut(s) 1344
ErhI CCWWGG 2 cut(s) 64, 561
Esp3I CGTCTC 1 cut(s) 987
FalI AAGNNNNNCTT 2 cut(s) 854, 886
FaqI GGGAC 2 cut(s) 272, 839
FblI GTMKAC 3 cut(s) 228, 1044, 1388
Fnu4HI GCNGC 8 cut(s) 315, 408, 543, 558, 567, 732, 1110, 1446
FokI GGATG 3 cut(s) 920, 1040, 1310
FriOI GRGCYC 1 cut(s) 1344
Fsp4HI GCNGC 8 cut(s) 315, 408, 543, 558, 567, 732, 1110, 1446
FspBI CTAG 2 cut(s) 471, 890
GlaI GCGC 4 cut(s) 657, 802, 1203, 1267
GluI GCNGC 8 cut(s) 315, 408, 543, 558, 567, 732, 1110, 1446
GsaI CCCAGC 1 cut(s) 826
GsuI CTGGAG 2 cut(s) 258, 369
HaeII RGCGCY 1 cut(s) 804
HaeIII GGCC 5 cut(s) 314, 763, 1121, 1342, 1448
HapII CCGG 5 cut(s) 255, 372, 840, 971, 1020
HgaI GACGC 3 cut(s) 16, 119, 1368
HhaI GCGC 4 cut(s) 658, 803, 1204, 1268
Hin1I GRCGYC 3 cut(s) 27, 111, 1379
Hin6I GCGC 4 cut(s) 656, 801, 1202, 1266
HinP1I GCGC 4 cut(s) 656, 801, 1202, 1266
HincII GTYRAC 4 cut(s) 229, 1081, 1261, 1320
HindII GTYRAC 4 cut(s) 229, 1081, 1261, 1320
HindIII AAGCTT 1 cut(s) 1439
HinfI GANTC 2 cut(s) 194, 1103
HpaI GTTAAC 1 cut(s) 1261
HpaII CCGG 5 cut(s) 255, 372, 840, 971, 1020
HphI GGTGA 5 cut(s) 109, 283, 890, 1009, 1166
Hpy166II GTNNAC 8 cut(s) 229, 1045, 1081, 1261, 1282, 1320, 1389, 1411
Hpy188I TCNGA 6 cut(s) 282, 587, 814, 1239, 1246, 1427
Hpy188III TCNNGA 2 cut(s) 785, 1291
Hpy8I GTNNAC 8 cut(s) 229, 1045, 1081, 1261, 1282, 1320, 1389, 1411
Hpy99I CGWCG 5 cut(s) 32, 35, 1337, 1384, 1387
HpyAV CCTTC 1 cut(s) 1288
HpyCH4III ACNGT 7 cut(s) 275, 295, 502, 808, 908, 917, 1098
HpyCH4IV ACGT 6 cut(s) 264, 363, 429, 1014, 1372, 1407
HpyCH4V TGCA 5 cut(s) 182, 487, 542, 575, 1401
HpyF3I CTNAG 3 cut(s) 77, 651, 813
HpySE526I ACGT 6 cut(s) 264, 363, 429, 1014, 1372, 1407
Hsp92I GRCGYC 3 cut(s) 27, 111, 1379
HspAI GCGC 4 cut(s) 656, 801, 1202, 1266
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
KspAI GTTAAC 1 cut(s) 1261
Kzo9I GATC 4 cut(s) 149, 595, 981, 1172
LmnI GCTCC 4 cut(s) 342, 842, 998, 1226
Lsp1109I GCAGC 3 cut(s) 553, 554, 743
LweI GCATC 2 cut(s) 241, 384
MaeI CTAG 2 cut(s) 471, 890
MaeII ACGT 6 cut(s) 264, 363, 429, 1014, 1372, 1407
MaeIII GTNAC 3 cut(s) 295, 917, 1015
MalI GATC 4 cut(s) 151, 597, 983, 1174
MboI GATC 4 cut(s) 149, 595, 981, 1172
MboII GAAGA 7 cut(s) 30, 33, 52, 269, 491, 520, 800
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 1 cut(s) 1172
MhlI GDGCHC 4 cut(s) 389, 686, 847, 1344
MluCI AATT 3 cut(s) 100, 537, 576
MlyI GAGTC 2 cut(s) 188, 1097
MmeI TCCRAC 4 cut(s) 418, 446, 1224, 1405
MroNI GCCGGC 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 2 cut(s) 831, 1260
MslI CAYNNNNRTG 1 cut(s) 1406
MspA1I CMGCKG 2 cut(s) 545, 1432
MspI CCGG 5 cut(s) 255, 372, 840, 971, 1020
MspR9I CCNGG 3 cut(s) 256, 528, 972
MunI CAATTG 1 cut(s) 576
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 2 cut(s) 1202, 1421
NaeI GCCGGC 1 cut(s) 373
NciI CCSGG 2 cut(s) 256, 972
NcoI CCATGG 1 cut(s) 561
NdeII GATC 4 cut(s) 149, 595, 981, 1172
NgoMIV GCCGGC 1 cut(s) 371
NlaIV GGNNCC 7 cut(s) 388, 827, 1000, 1195, 1236, 1327, 1342
NmuCI GTSAC 2 cut(s) 295, 1015
NspI RCATGY 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 1406
PaeI GCATGC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 1341
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 363
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 8 cut(s) 316, 409, 544, 559, 568, 733, 1111, 1447
PleI GAGTC 2 cut(s) 188, 1097
PpsI GAGTC 2 cut(s) 188, 1097
Ppu21I YACGTR 1 cut(s) 1408
PpuMI RGGWCCY 1 cut(s) 826
PsiI TTATAA 1 cut(s) 105
Psp1406I AACGTT 2 cut(s) 363, 1372
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 822
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 7 cut(s) 388, 827, 1000, 1195, 1236, 1327, 1342
PspOMI GGGCCC 1 cut(s) 1340
PspPI GGNCC 7 cut(s) 312, 762, 826, 1193, 1340, 1341, 1427
PspPPI RGGWCCY 1 cut(s) 826
PsuI RGATCY 1 cut(s) 1172
PsyI GACNNNGTC 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 4 cut(s) 272, 746, 779, 1283
RsaNI GTAC 4 cut(s) 271, 745, 778, 1282
RseI CAYNNNNRTG 1 cut(s) 1406
Rsr2I CGGWCCG 1 cut(s) 1427
RsrII CGGWCCG 1 cut(s) 1427
SalI GTCGAC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 831, 1260
SatI GCNGC 8 cut(s) 315, 408, 543, 558, 567, 732, 1110, 1446
Sau3AI GATC 4 cut(s) 149, 595, 981, 1172
Sau96I GGNCC 7 cut(s) 312, 762, 826, 1193, 1340, 1341, 1427
SchI GAGTC 2 cut(s) 188, 1097
ScrFI CCNGG 3 cut(s) 256, 528, 972
SduI GDGCHC 4 cut(s) 389, 686, 847, 1344
SfaNI GCATC 2 cut(s) 241, 384
SfcI CTRYAG 1 cut(s) 1045
SinI GGWCC 3 cut(s) 826, 1193, 1427
SmiMI CAYNNNNRTG 1 cut(s) 1406
SmlI CTYRAG 3 cut(s) 137, 773, 988
SmoI CTYRAG 3 cut(s) 137, 773, 988
SphI GCATGC 1 cut(s) 51
Sse9I AATT 3 cut(s) 100, 537, 576
SspMI CTAG 2 cut(s) 471, 890
StyD4I CCNGG 3 cut(s) 254, 526, 970
StyI CCWWGG 2 cut(s) 64, 561
TaaI ACNGT 7 cut(s) 275, 295, 502, 808, 908, 917, 1098
TaiI ACGT 6 cut(s) 267, 366, 432, 1017, 1375, 1410
TaqI TCGA 4 cut(s) 204, 228, 1101, 1362
TasI AATT 3 cut(s) 100, 537, 576
TatI WGTACW 3 cut(s) 744, 777, 1281
TauI GCSGC 5 cut(s) 317, 410, 560, 1112, 1448
Tru1I TTAA 2 cut(s) 831, 1260
Tru9I TTAA 2 cut(s) 831, 1260
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 2 cut(s) 295, 1015
TseI GCWGC 3 cut(s) 542, 566, 731
Tsp45I GTSAC 2 cut(s) 295, 1015
TspDTI ATGAA 2 cut(s) 74, 474
TspGWI ACGGA 1 cut(s) 1011
TspRI CASTG 1 cut(s) 97
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 3 cut(s) 826, 1193, 1427
XceI RCATGY 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 1135
XmiI GTMKAC 3 cut(s) 228, 1044, 1388
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 2 cut(s) 471, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.