MD15G1049000.v1.1

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008332
Physical Location & Seq
Forward (+)
3331387 .. 3333871
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1049000.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGTTTGGTGGAAGAAGAGGCGTCGCCGCCACCGTTGTGGCATGCCTTGCTCTCTTCCAAGGACTTGTTCTTAGCGTTCATGGCACTGGTATTAATTACAAGGACGCTCTCACCAAGTCCATTATTTTCCTTGAAGCTCAAAGATCAGGGAAACTCCCTCCCAATCATAGACCTGCATGGAGAGGCGACTCTGCCCTCGACGATGGAAAAGAAGCAAATGTCGACCTTGCCGGAGGATACTATGATGCCGGAGACAACGTCAAGTACGGTCTTCCGATGGCATTCACCGTCACAACTCTGTCGTGGGCTGCCATGTTTTACAGGCAACAGCTCGAAGCTACTGGAGAACTGGAAAATGTTCTTGCCGGCATCAAATGGGGCACTGATTATTTTCTGAAAGCCACTCGAAAAAACAGATTATACGTTCAGGTTGGAGACCCAAATCAAGACCATCAATGTTGGACTAGACCAGAAGATATGCAGACACCAAGAACAGTGTTGAAGATTGATGATAGCACACCCGGAACTGAAATTGCAGCTGAAACTTCAGCCGCCATGGCTGCCTCTTCAATTGTGTTCAGAAAAACAGATGGTGTCTATGCTCGTAGTCTACTCAACAAGGCCAAACGTCTCTTTCAAATGGCTAAGGCGCATAAGGGAACTTACGATGGAGAGTGCCCTTTCTACTGCTCTTACTCTGGCTATAATGATGAGCTGTTGTGGGCAGCAACATGGTTGTACATTGCCACAAAGAGGCCCTTGTATCTCAAGTACATTCAGGAAGAAGCCATCAGCGCCACCGTGTCTGAGTTCAGCTGGGACCTTAAATACGCCGGAGCACAAGTCCTCCTGGCCAAGCTGTTCTTTGAAGGTGAAAAATCCCTAGAGAACTACAAAAACGGTGCTGACAGTTACATTTGCTCAAACATCCCCGACAGTCCTTACAACCAAGTCACAATGACACCGGGCGGCATGATCCACTTGAGAGACGGAGCCAACACCCAATACGTCACCGGAACCGCCCACTTATTCGCCGTCTACAGCGATATCCTCGCCCAGCACAACCAAAAAGTCAACTGCGGAGGCAAAGAGTTGGACTCAGGCGCCCTCCTCGCTTTTGCCAAGAAGCAAATGGACTACTTGTTGGGTGAGAACCCAAAAGGAAGATCCTACATGGTCGGGTTCGGACCCAACGCGCCCCAACAGCCCCACCACAGAGGAGCTTCGGTACCGAAGGGGGAAAACGGCATCGTTAACTGCGCCATGAGCTTCGTGAACTGGTTCAAGAAGGACGTGCCAAACCCTAATGAGTTGACTGGTGCCATCGTCGGGGGGCCCGATAGGTTTGATAATTTCGAGGACAAACGTTGGGCGTCGTCGTATACCGAGCCATGCACTTACGTGAACTCGCTCGCGGTCGGACCGCTGGCGAAGCTTGCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

482

Amino Acids

52.92

Weight (kDa)

7.09

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 474 2.6e-152 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 181
Acc65I GGTACC 1 cut(s) 1228
AccB1I GGYRCC 3 cut(s) 1103, 1228, 1319
AccI GTMKAC 4 cut(s) 222, 610, 1038, 1382
AccII CGCG 2 cut(s) 1196, 1415
AciI CCGC 7 cut(s) 27, 552, 969, 1020, 1080, 1415, 1424
AclI AACGTT 1 cut(s) 1366
AclWI GGATC 2 cut(s) 970, 1161
AcoI YGGCCR 1 cut(s) 852
AcuI CTGAAG 1 cut(s) 531
AcyI GRCGYC 3 cut(s) 21, 1104, 1373
AfaI GTAC 4 cut(s) 266, 740, 773, 1230
AfiI CCNNNNNNNGG 2 cut(s) 753, 1329
AgsI TTSAA 6 cut(s) 134, 502, 570, 638, 869, 1285
AjiI CACGTC 1 cut(s) 1294
AjnI CCWGG 1 cut(s) 849
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AleI CACNNNNGTG 2 cut(s) 35, 1400
Alw21I GWGCWC 1 cut(s) 841
Alw26I GTCTC 4 cut(s) 246, 429, 635, 981
AlwI GGATC 2 cut(s) 970, 1161
AoxI GGCC 4 cut(s) 621, 755, 852, 1334
ApaI GGGCCC 1 cut(s) 1338
ApeKI GCWGC 4 cut(s) 308, 536, 560, 725
AseI ATTAAT 1 cut(s) 93
Asp700I GAANNNNTTC 2 cut(s) 357, 1280
Asp718I GGTACC 1 cut(s) 1228
AspLEI GCGC 5 cut(s) 652, 797, 1106, 1198, 1262
AspS9I GGNCC 6 cut(s) 756, 820, 1187, 1334, 1335, 1421
AsuC2I CCSGG 2 cut(s) 522, 966
AsuHPI GGTGA 5 cut(s) 103, 277, 884, 1003, 1160
AvaII GGWCC 3 cut(s) 820, 1187, 1421
BaeGI GKGCMC 3 cut(s) 383, 680, 1338
BalI TGGCCA 1 cut(s) 854
BanI GGYRCC 3 cut(s) 1103, 1228, 1319
BanII GRGCYC 1 cut(s) 1338
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 841
BbvI GCAGC 4 cut(s) 295, 547, 548, 737
BccI CCATC 7 cut(s) 197, 271, 459, 584, 662, 797, 1331
BceAI ACGGC 2 cut(s) 1019, 1261
BciT130I CCWGG 1 cut(s) 851
BciVI GTATCC 1 cut(s) 230
BcnI CCSGG 2 cut(s) 522, 966
BcoDI GTCTC 4 cut(s) 246, 429, 635, 981
BfaI CTAG 2 cut(s) 465, 884
BfmI CTRYAG 1 cut(s) 1039
BfoI RGCGCY 2 cut(s) 798, 1107
BfuAI ACCTGC 1 cut(s) 181
BfuI GTATCC 1 cut(s) 230
BglI GCCNNNNNGGC 1 cut(s) 557
BisI GCNGC 7 cut(s) 27, 309, 537, 552, 561, 726, 970
BlsI GCNGC 7 cut(s) 28, 310, 538, 553, 562, 727, 971
Bme1390I CCNGG 3 cut(s) 522, 851, 966
Bme18I GGWCC 3 cut(s) 820, 1187, 1421
BmgBI CACGTC 1 cut(s) 1294
BmgT120I GGNCC 6 cut(s) 756, 820, 1187, 1334, 1335, 1421
BmiI GGNNCC 9 cut(s) 821, 994, 1018, 1105, 1189, 1230, 1321, 1335, 1336
BmrFI CCNGG 3 cut(s) 522, 851, 966
BmsI GCATC 3 cut(s) 235, 378, 1257
BpiI GAAGAC 1 cut(s) 263
BplI GAGNNNNNCTC 2 cut(s) 1082, 1114
BpmI CTGGAG 1 cut(s) 363
Bpu10I CCTNAGC 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 752, 1003
BpuMI CCSGG 2 cut(s) 522, 966
BsaAI YACGTR 1 cut(s) 1402
BsaHI GRCGYC 3 cut(s) 21, 1104, 1373
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 58, 555
BsaWI WCCGGW 1 cut(s) 1013
BsaXI ACNNNNNCTCC 8 cut(s) 243, 273, 828, 831, 858, 861, 1212, 1242
Bsc4I CCNNNNNNNGG 2 cut(s) 753, 1329
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 5 cut(s) 91, 346, 354, 1283, 1321
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 1 cut(s) 851
BseDI CCNNGG 2 cut(s) 58, 555
BseGI GGATG 1 cut(s) 927
BseLI CCNNNNNNNGG 2 cut(s) 753, 1329
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 2 cut(s) 798, 1113
BseNI ACTGG 5 cut(s) 91, 346, 354, 1283, 1321
BseRI GAGGAG 2 cut(s) 1100, 1233
BseSI GKGCMC 3 cut(s) 383, 680, 1338
BseXI GCAGC 4 cut(s) 295, 547, 548, 737
BseYI CCCAGC 2 cut(s) 816, 1056
Bsh1236I CGCG 2 cut(s) 1196, 1415
Bsh1285I CGRYCG 1 cut(s) 1419
BshFI GGCC 4 cut(s) 623, 757, 854, 1336
BshNI GGYRCC 3 cut(s) 1103, 1228, 1319
BsiEI CGRYCG 1 cut(s) 1419
BsiHKAI GWGCWC 1 cut(s) 841
BsiSI CCGG 7 cut(s) 231, 249, 366, 522, 834, 965, 1014
BslFI GGGAC 1 cut(s) 833
BslI CCNNNNNNNGG 2 cut(s) 753, 1329
BsmAI GTCTC 4 cut(s) 246, 429, 635, 981
BsmBI CGTCTC 2 cut(s) 635, 981
BsmFI GGGAC 1 cut(s) 833
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 4 cut(s) 623, 757, 854, 1336
Bso31I GGTCTC 1 cut(s) 429
Bsp120I GGGCCC 1 cut(s) 1334
Bsp1286I GDGCHC 4 cut(s) 383, 680, 841, 1338
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 3 cut(s) 143, 975, 1166
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 7 cut(s) 27, 552, 969, 1020, 1080, 1415, 1424
BspANI GGCC 4 cut(s) 623, 757, 854, 1336
BspCNI CTCAG 2 cut(s) 799, 1112
BspFNI CGCG 2 cut(s) 1196, 1415
BspLI GGNNCC 9 cut(s) 821, 994, 1018, 1105, 1189, 1230, 1321, 1335, 1336
BspMI ACCTGC 1 cut(s) 181
BspPI GGATC 2 cut(s) 970, 1161
BspT107I GGYRCC 3 cut(s) 1103, 1228, 1319
BspTNI GGTCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 741
BsrFI RCCGGY 1 cut(s) 365
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 5 cut(s) 91, 346, 354, 1283, 1321
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 58, 555
BssMI GATC 3 cut(s) 143, 975, 1166
BssNAI GTATAC 1 cut(s) 1383
BssNI GRCGYC 3 cut(s) 21, 1104, 1373
BssT1I CCWWGG 2 cut(s) 58, 555
Bst1107I GTATAC 1 cut(s) 1383
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 8 cut(s) 34, 269, 289, 496, 802, 902, 911, 938
Bst6I CTCTTC 3 cut(s) 10, 59, 571
BstACI GRCGYC 3 cut(s) 21, 1104, 1373
BstAPI GCANNNNNTGC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 738
BstBAI YACGTR 1 cut(s) 1402
BstC8I GCNNGC 5 cut(s) 43, 367, 1413, 1428, 1437
BstDEI CTNAG 4 cut(s) 71, 645, 807, 1099
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 1 cut(s) 927
BstFNI CGCG 2 cut(s) 1196, 1415
BstH2I RGCGCY 2 cut(s) 798, 1107
BstHHI GCGC 5 cut(s) 652, 797, 1106, 1198, 1262
BstKTI GATC 3 cut(s) 146, 978, 1169
BstMAI GTCTC 4 cut(s) 246, 429, 635, 981
BstMBI GATC 3 cut(s) 143, 975, 1166
BstMCI CGRYCG 1 cut(s) 1419
BstNI CCWGG 1 cut(s) 851
BstNSI RCATGY 1 cut(s) 45
BstSCI CCNGG 3 cut(s) 520, 849, 964
BstSFI CTRYAG 1 cut(s) 1039
BstSLI GKGCMC 3 cut(s) 383, 680, 1338
BstUI CGCG 2 cut(s) 1196, 1415
BstV1I GCAGC 4 cut(s) 295, 547, 548, 737
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 1 cut(s) 1166
BstXI CCANNNNNNTGG 1 cut(s) 37
BstYI RGATCY 1 cut(s) 1166
BstZ17I GTATAC 1 cut(s) 1383
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 4 cut(s) 623, 757, 854, 1336
BtgI CCRYGG 1 cut(s) 555
BtrI CACGTC 1 cut(s) 1294
BtsCI GGATG 1 cut(s) 927
BtsIMutI CAGTG 3 cut(s) 84, 381, 501
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 5 cut(s) 43, 367, 1413, 1428, 1437
CfoI GCGC 5 cut(s) 652, 797, 1106, 1198, 1262
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 6 cut(s) 756, 820, 1187, 1334, 1335, 1421
CpoI CGGWCCG 1 cut(s) 1421
CseI GACGC 3 cut(s) 10, 113, 1362
Csp6I GTAC 4 cut(s) 265, 739, 772, 1229
CspI CGGWCCG 1 cut(s) 1421
CviQI GTAC 4 cut(s) 265, 739, 772, 1229
DdeI CTNAG 4 cut(s) 71, 645, 807, 1099
DinI GGCGCC 1 cut(s) 1105
DpnI GATC 3 cut(s) 145, 977, 1168
DpnII GATC 3 cut(s) 143, 975, 1166
EaeI YGGCCR 1 cut(s) 852
Eam1104I CTCTTC 3 cut(s) 10, 59, 571
EarI CTCTTC 3 cut(s) 10, 59, 571
Eco130I CCWWGG 2 cut(s) 58, 555
Eco24I GRGCYC 1 cut(s) 1338
Eco31I GGTCTC 1 cut(s) 429
Eco32I GATATC 1 cut(s) 1048
Eco47I GGWCC 3 cut(s) 820, 1187, 1421
Eco57I CTGAAG 1 cut(s) 531
EcoO109I RGGNCCY 3 cut(s) 756, 820, 1334
EcoRII CCWGG 1 cut(s) 849
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 2 cut(s) 58, 555
EcoT38I GRGCYC 1 cut(s) 1338
EgeI GGCGCC 1 cut(s) 1105
EheI GGCGCC 1 cut(s) 1105
ErhI CCWWGG 2 cut(s) 58, 555
Esp3I CGTCTC 2 cut(s) 635, 981
FalI AAGNNNNNCTT 4 cut(s) 743, 775, 848, 880
FaqI GGGAC 1 cut(s) 833
FblI GTMKAC 4 cut(s) 222, 610, 1038, 1382
Fnu4HI GCNGC 7 cut(s) 27, 309, 537, 552, 561, 726, 970
FokI GGATG 1 cut(s) 914
FriOI GRGCYC 1 cut(s) 1338
Fsp4HI GCNGC 7 cut(s) 27, 309, 537, 552, 561, 726, 970
FspBI CTAG 2 cut(s) 465, 884
GlaI GCGC 5 cut(s) 651, 796, 1105, 1197, 1261
GluI GCNGC 7 cut(s) 27, 309, 537, 552, 561, 726, 970
GsaI CCCAGC 2 cut(s) 820, 1060
GsuI CTGGAG 1 cut(s) 363
HaeII RGCGCY 2 cut(s) 798, 1107
HaeIII GGCC 4 cut(s) 623, 757, 854, 1336
HapII CCGG 7 cut(s) 231, 249, 366, 522, 834, 965, 1014
HgaI GACGC 3 cut(s) 10, 113, 1362
HhaI GCGC 5 cut(s) 652, 797, 1106, 1198, 1262
Hin1I GRCGYC 3 cut(s) 21, 1104, 1373
Hin6I GCGC 5 cut(s) 650, 795, 1104, 1196, 1260
HinP1I GCGC 5 cut(s) 650, 795, 1104, 1196, 1260
HincII GTYRAC 4 cut(s) 223, 1075, 1255, 1314
HindII GTYRAC 4 cut(s) 223, 1075, 1255, 1314
HindIII AAGCTT 1 cut(s) 1433
HinfI GANTC 2 cut(s) 188, 1097
HpaI GTTAAC 1 cut(s) 1255
HpaII CCGG 7 cut(s) 231, 249, 366, 522, 834, 965, 1014
HphI GGTGA 5 cut(s) 103, 277, 884, 1003, 1160
Hpy166II GTNNAC 9 cut(s) 223, 611, 1039, 1075, 1255, 1276, 1314, 1383, 1405
Hpy188I TCNGA 6 cut(s) 276, 396, 581, 808, 1187, 1421
Hpy188III TCNNGA 4 cut(s) 446, 779, 1273, 1285
Hpy8I GTNNAC 9 cut(s) 223, 611, 1039, 1075, 1255, 1276, 1314, 1383, 1405
Hpy99I CGWCG 5 cut(s) 26, 203, 1331, 1378, 1381
HpyAV CCTTC 3 cut(s) 863, 1228, 1282
HpyCH4III ACNGT 8 cut(s) 34, 269, 289, 496, 802, 902, 911, 938
HpyCH4IV ACGT 7 cut(s) 258, 423, 628, 1008, 1293, 1366, 1401
HpyCH4V TGCA 4 cut(s) 176, 481, 536, 1395
HpyF3I CTNAG 4 cut(s) 71, 645, 807, 1099
HpySE526I ACGT 7 cut(s) 258, 423, 628, 1008, 1293, 1366, 1401
Hsp92I GRCGYC 3 cut(s) 21, 1104, 1373
HspAI GCGC 5 cut(s) 650, 795, 1104, 1196, 1260
KasI GGCGCC 1 cut(s) 1103
KpnI GGTACC 1 cut(s) 1232
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
KspAI GTTAAC 1 cut(s) 1255
Kzo9I GATC 3 cut(s) 143, 975, 1166
LmnI GCTCC 3 cut(s) 836, 992, 1220
Lsp1109I GCAGC 4 cut(s) 295, 547, 548, 737
LweI GCATC 3 cut(s) 235, 378, 1257
MaeI CTAG 2 cut(s) 465, 884
MaeII ACGT 7 cut(s) 258, 423, 628, 1008, 1293, 1366, 1401
MaeIII GTNAC 4 cut(s) 289, 911, 952, 1009
MalI GATC 3 cut(s) 145, 977, 1168
MboI GATC 3 cut(s) 143, 975, 1166
MboII GAAGA 9 cut(s) 24, 27, 46, 263, 485, 514, 558, 794, 1176
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 1 cut(s) 1166
MhlI GDGCHC 4 cut(s) 383, 680, 841, 1338
MlsI TGGCCA 1 cut(s) 854
MluCI AATT 4 cut(s) 94, 531, 570, 1351
MluNI TGGCCA 1 cut(s) 854
Mly113I GGCGCC 1 cut(s) 1104
MlyI GAGTC 2 cut(s) 182, 1091
MmeI TCCRAC 4 cut(s) 412, 440, 1074, 1399
Mox20I TGGCCA 1 cut(s) 854
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 2 cut(s) 357, 1280
MscI TGGCCA 1 cut(s) 854
MseI TTAA 3 cut(s) 93, 825, 1254
MslI CAYNNNNRTG 2 cut(s) 35, 1400
Msp20I TGGCCA 1 cut(s) 854
MspA1I CMGCKG 3 cut(s) 539, 816, 1426
MspI CCGG 7 cut(s) 231, 249, 366, 522, 834, 965, 1014
MspR9I CCNGG 3 cut(s) 522, 851, 966
MunI CAATTG 1 cut(s) 570
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 1 cut(s) 851
MvnI CGCG 2 cut(s) 1196, 1415
NaeI GCCGGC 1 cut(s) 367
NarI GGCGCC 1 cut(s) 1104
NciI CCSGG 2 cut(s) 522, 966
NcoI CCATGG 1 cut(s) 555
NdeII GATC 3 cut(s) 143, 975, 1166
NgoMIV GCCGGC 1 cut(s) 365
NlaIV GGNNCC 9 cut(s) 821, 994, 1018, 1105, 1189, 1230, 1321, 1335, 1336
NmuCI GTSAC 3 cut(s) 289, 952, 1009
NspI RCATGY 1 cut(s) 45
OliI CACNNNNGTG 2 cut(s) 35, 1400
PaeI GCATGC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 1335
PctI GAATGC 1 cut(s) 281
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 2 cut(s) 357, 1280
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 7 cut(s) 28, 310, 538, 553, 562, 727, 971
PleI GAGTC 2 cut(s) 182, 1091
PluTI GGCGCC 1 cut(s) 1107
PpsI GAGTC 2 cut(s) 182, 1091
Ppu21I YACGTR 1 cut(s) 1402
PpuMI RGGWCCY 1 cut(s) 820
PshBI ATTAAT 1 cut(s) 93
Psp1406I AACGTT 1 cut(s) 1366
Psp5II RGGWCCY 1 cut(s) 820
Psp6I CCWGG 1 cut(s) 849
PspFI CCCAGC 2 cut(s) 816, 1056
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 9 cut(s) 821, 994, 1018, 1105, 1189, 1230, 1321, 1335, 1336
PspOMI GGGCCC 1 cut(s) 1334
PspPI GGNCC 6 cut(s) 756, 820, 1187, 1334, 1335, 1421
PspPPI RGGWCCY 1 cut(s) 820
PsuI RGATCY 1 cut(s) 1166
PsyI GACNNNGTC 1 cut(s) 257
PvuII CAGCTG 2 cut(s) 539, 816
RsaI GTAC 4 cut(s) 266, 740, 773, 1230
RsaNI GTAC 4 cut(s) 265, 739, 772, 1229
RseI CAYNNNNRTG 2 cut(s) 35, 1400
Rsr2I CGGWCCG 1 cut(s) 1421
RsrII CGGWCCG 1 cut(s) 1421
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 3 cut(s) 93, 825, 1254
SatI GCNGC 7 cut(s) 27, 309, 537, 552, 561, 726, 970
Sau3AI GATC 3 cut(s) 143, 975, 1166
Sau96I GGNCC 6 cut(s) 756, 820, 1187, 1334, 1335, 1421
SchI GAGTC 2 cut(s) 182, 1091
ScrFI CCNGG 3 cut(s) 522, 851, 966
SduI GDGCHC 4 cut(s) 383, 680, 841, 1338
SfaNI GCATC 3 cut(s) 235, 378, 1257
SfcI CTRYAG 1 cut(s) 1039
SfoI GGCGCC 1 cut(s) 1105
SinI GGWCC 3 cut(s) 820, 1187, 1421
SmiMI CAYNNNNRTG 2 cut(s) 35, 1400
SmlI CTYRAG 2 cut(s) 767, 982
SmoI CTYRAG 2 cut(s) 767, 982
SphI GCATGC 1 cut(s) 45
Sse9I AATT 4 cut(s) 94, 531, 570, 1351
SsiI CCGC 7 cut(s) 27, 552, 969, 1020, 1080, 1415, 1424
SspDI GGCGCC 1 cut(s) 1103
SspMI CTAG 2 cut(s) 465, 884
StyD4I CCNGG 3 cut(s) 520, 849, 964
StyI CCWWGG 2 cut(s) 58, 555
TaaI ACNGT 8 cut(s) 34, 269, 289, 496, 802, 902, 911, 938
TaiI ACGT 7 cut(s) 261, 426, 631, 1011, 1296, 1369, 1404
TaqI TCGA 5 cut(s) 198, 222, 333, 406, 1356
TasI AATT 4 cut(s) 94, 531, 570, 1351
TatI WGTACW 2 cut(s) 738, 771
TauI GCSGC 3 cut(s) 29, 554, 972
Tru1I TTAA 3 cut(s) 93, 825, 1254
Tru9I TTAA 3 cut(s) 93, 825, 1254
TscAI CASTG 3 cut(s) 91, 388, 501
TseFI GTSAC 3 cut(s) 289, 952, 1009
TseI GCWGC 4 cut(s) 308, 536, 560, 725
Tsp45I GTSAC 3 cut(s) 289, 952, 1009
TspDTI ATGAA 1 cut(s) 68
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 3 cut(s) 91, 388, 501
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 820, 1187, 1421
VspI ATTAAT 1 cut(s) 93
XceI RCATGY 1 cut(s) 45
XcmI CCANNNNNNNNNTGG 1 cut(s) 1129
XmiI GTMKAC 4 cut(s) 222, 610, 1038, 1382
XmnI GAANNNNTTC 2 cut(s) 357, 1280
XspI CTAG 2 cut(s) 465, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.