MD08G1118800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016771
Physical Location & Seq
Reverse (-)
10837938 .. 10840075
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1118800.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGCGTTGGTCTGCCGAGTTTTATCGGCTGCGAGAAGGGGTTTAAAGCACAAGGCTTCGGCGAGTAGCCCTCACTCTCGGCGTTGGCTGAGCAACCAAACAAGCTTTCTTCTTGGGAGGTTGGTGGAGGAACCCAATTCACGCATCAAATCCTTACTCTCTTCCGATGAATTCTCCGCCCTCCAAAGCTCGGATTTTTCCTGGGAATCCCTCGTCAGTTCTCTCGCTTCTTCTTCCTCTCCCGGGAAATCCCGATTGGTTTTGGAGTGGAAACTGGAGAAGTTGCTCGGGGCGAACGAGAGAGATCATGATCGTTACTCCGAGCTGATATCTCTGTGTGGAAAAATTCACAATCTCCCACTTGCAATGCAAGTGTTTTCTTCTATGGAGGCTAATGGAATCAAACCCACTTCTGCCGTTTTCAACTCCCTTATACATGTTTGCTTCTCTTCCGGTAATGTGTTGACAGCTTTAAGCCTATTTGAGATAATGAAGAGCTCGGAGGGATTTAAACCCGATTCCGACACATATGATGCATTCATGTTTGCATTCTCAAAGTTGGGCAATGCTGATTCTTTGCAAGCTTGGTACTCGGCCAAGAAGGCTGCCGGATTCACTTCTGGTGTTCAAACCTATGAATATCTGATTTCGGGTTGCATTAAATCAAACAATTTTGAGTTGGGTGATGGGTTCTATGAAGAAATGGTGTTATCAGGGCACATGCCAAGCTTGCCCGTATTGGAAAGTATGGTGGAAGGGATTTGTAAACGGAGAAACTTTGATTTAGTAATAGACTTTTTGAAGATAGTGTTAGATGCCGGATTGAAGATCAACGAGAAGATGGCTGAGATGGTTGTGGGATTGTACATTGAACTTAAGATGGTGGAGAAATTGGAGGAGTTGCTTGTAATTCTTACGGAGGCCAATCAAGTTTCGGAAGTTCTGTTGCTGGTCCACTGTGGAATTATACGGTTGAATGCCGCATTGGATAGATTGGATGATGTAGAGTACTCTGTTGGGAGGATGTTGAAGCAAGGATTGTCATTTAAACATCAGGATGATGTCGAAAAGGTAATCTGTTCGTACTTCAGGTGCTCGGCTCATGACAGGCTGGAGTTGTTTTTGGAACGTATTGAGGGTTCTTATGAACTTACGACATCAACTTATGATCTGTTGGTTGCCGGCTATCGAAGAGCAGGATTATCTGACAGGTTGAACGACATGAAATTAGCTGAAAAAAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

416

Amino Acids

46.42

Weight (kDa)

5.93

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 105 - 148 1.8e-07 Pentatricopeptide repeat domain
PPR_long PF17177 106 - 192 9.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 136 - 185 5.5e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016771)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36160
malus_domestica MD08G1118800.v1.1
prunus_persica Prupe.1G453000_v2.0.a1
pyrus_communis pycom08g09920
rosa_chinensis RchiOBHm_Chr6g0305711
rosa_laevigata RLG00000010866
rosa_multiflora Rmu_sc0001809.1_g000036
rosa_roxburghii Rroxscaffold_7G00162420
rosa_rugosa Rorug06G0343400
rosa_samantha Rh6AG454900 Rh6BG446600 Rh6CG468800 Rh6DG455400
rosa_wichuraiana Rw6G039670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 177, 981
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 2 cut(s) 170, 345
AcuI CTGAAG 1 cut(s) 1072
AfaI GTAC 4 cut(s) 590, 866, 1010, 1085
AfiI CCNNNNNNNGG 2 cut(s) 190, 243
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 436
AgsI TTSAA 8 cut(s) 424, 629, 802, 826, 872, 976, 1030, 1216
AjnI CCWGG 1 cut(s) 200
AjuI GAANNNNNNNTTGG 6 cut(s) 90, 122, 239, 271, 968, 1000
AluBI AGCT 8 cut(s) 105, 189, 325, 470, 498, 584, 729, 1232
AluI AGCT 8 cut(s) 105, 189, 325, 470, 498, 584, 729, 1232
Alw21I GWGCWC 2 cut(s) 500, 1097
Ama87I CYCGRG 2 cut(s) 242, 287
AoxI GGCC 2 cut(s) 594, 921
ApeKI GCWGC 2 cut(s) 29, 605
ApoI RAATTY 2 cut(s) 170, 345
AspS9I GGNCC 1 cut(s) 952
AsuC2I CCSGG 2 cut(s) 243, 244
AsuHPI GGTGA 1 cut(s) 695
AvaI CYCGRG 2 cut(s) 242, 287
AvaII GGWCC 1 cut(s) 952
BaeGI GKGCMC 1 cut(s) 720
BanII GRGCYC 1 cut(s) 500
Bbv12I GWGCWC 2 cut(s) 500, 1097
BbvI GCAGC 2 cut(s) 16, 592
BccI CCATC 4 cut(s) 680, 835, 844, 874
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 1 cut(s) 202
BcnI CCSGG 2 cut(s) 243, 244
BfrI CTTAAG 1 cut(s) 875
BglI GCCNNNNNGGC 1 cut(s) 602
BisI GCNGC 3 cut(s) 30, 606, 981
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 3 cut(s) 31, 607, 982
BmcAI AGTACT 1 cut(s) 1010
Bme1390I CCNGG 3 cut(s) 202, 243, 244
Bme18I GGWCC 1 cut(s) 952
BmeT110I CYCGRG 2 cut(s) 242, 287
BmgT120I GGNCC 1 cut(s) 952
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 3 cut(s) 202, 243, 244
BmsI GCATC 3 cut(s) 153, 523, 805
BplI GAGNNNNNCTC 2 cut(s) 55, 87
BpmI CTGGAG 2 cut(s) 296, 1133
Bpu1102I GCTNAGC 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 243, 244
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 201, 242
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 243
Bse118I RCCGGY 1 cut(s) 1181
Bse1I ACTGG 1 cut(s) 279
Bse3DI GCAATG 2 cut(s) 372, 571
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 2 cut(s) 201, 242
BseGI GGATG 3 cut(s) 1003, 1029, 1063
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 2 cut(s) 190, 243
BseMI GCAATG 2 cut(s) 372, 571
BseMII CTCAG 2 cut(s) 80, 837
BseNI ACTGG 1 cut(s) 279
BseRI GAGGAG 1 cut(s) 911
BseSI GKGCMC 1 cut(s) 720
BseXI GCAGC 2 cut(s) 16, 592
BshFI GGCC 2 cut(s) 596, 923
BsiHKAI GWGCWC 2 cut(s) 500, 1097
BsiHKCI CYCGRG 2 cut(s) 242, 287
BsiSI CCGG 5 cut(s) 243, 453, 609, 819, 1182
BslI CCNNNNNNNGG 2 cut(s) 190, 243
BsmI GAATGC 3 cut(s) 536, 548, 982
BsnI GGCC 2 cut(s) 596, 923
BsoBI CYCGRG 2 cut(s) 242, 287
Bsp1286I GDGCHC 3 cut(s) 500, 720, 1097
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 4 cut(s) 304, 310, 828, 1168
Bsp1720I GCTNAGC 1 cut(s) 89
BspACI CCGC 2 cut(s) 177, 981
BspANI GGCC 2 cut(s) 596, 923
BspCNI CTCAG 2 cut(s) 81, 838
BspHI TCATGA 2 cut(s) 307, 1102
BspLI GGNNCC 1 cut(s) 132
BspQI GCTCTTC 2 cut(s) 488, 1186
BspTI CTTAAG 1 cut(s) 875
BsrDI GCAATG 2 cut(s) 372, 571
BsrFI RCCGGY 1 cut(s) 1181
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 1 cut(s) 279
BssAI RCCGGY 1 cut(s) 1181
BssECI CCNNGG 2 cut(s) 201, 242
BssMI GATC 4 cut(s) 304, 310, 828, 1168
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 2 cut(s) 959, 972
Bst6I CTCTTC 4 cut(s) 166, 454, 488, 1186
BstAFI CTTAAG 1 cut(s) 875
BstAUI TGTACA 1 cut(s) 864
BstC8I GCNNGC 3 cut(s) 582, 731, 1183
BstDEI CTNAG 2 cut(s) 89, 846
BstF5I GGATG 3 cut(s) 1003, 1029, 1063
BstKTI GATC 4 cut(s) 307, 313, 831, 1171
BstMBI GATC 4 cut(s) 304, 310, 828, 1168
BstMWI GCNNNNNNNGC 2 cut(s) 602, 730
BstNI CCWGG 1 cut(s) 202
BstNSI RCATGY 2 cut(s) 440, 724
BstSCI CCNGG 3 cut(s) 200, 241, 242
BstSLI GKGCMC 1 cut(s) 720
BstV1I GCAGC 2 cut(s) 16, 592
BsuRI GGCC 2 cut(s) 596, 923
BtsCI GGATG 3 cut(s) 1003, 1029, 1063
BtsIMutI CAGTG 1 cut(s) 955
Cac8I GCNNGC 3 cut(s) 582, 731, 1183
CciI TCATGA 2 cut(s) 307, 1102
Cfr10I RCCGGY 1 cut(s) 1181
Cfr13I GGNCC 1 cut(s) 952
Cfr9I CCCGGG 1 cut(s) 242
Csp6I GTAC 4 cut(s) 589, 865, 1009, 1084
CviAII CATG 6 cut(s) 308, 437, 541, 721, 1103, 1222
CviQI GTAC 4 cut(s) 589, 865, 1009, 1084
DdeI CTNAG 2 cut(s) 89, 846
DpnI GATC 4 cut(s) 306, 312, 830, 1170
DpnII GATC 4 cut(s) 304, 310, 828, 1168
DraI TTTAAA 3 cut(s) 45, 511, 1048
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 4 cut(s) 166, 454, 488, 1186
EarI CTCTTC 4 cut(s) 166, 454, 488, 1186
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 500
Eco32I GATATC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 952
Eco53kI GAGCTC 1 cut(s) 498
Eco57I CTGAAG 1 cut(s) 1072
Eco88I CYCGRG 2 cut(s) 242, 287
EcoICRI GAGCTC 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 200
EcoRV GATATC 1 cut(s) 330
EcoT22I ATGCAT 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 500
FaeI CATG 6 cut(s) 311, 440, 544, 724, 1106, 1225
FatI CATG 6 cut(s) 307, 436, 540, 720, 1102, 1221
FauNDI CATATG 1 cut(s) 529
Fnu4HI GCNGC 3 cut(s) 30, 606, 981
FokI GGATG 3 cut(s) 1010, 1036, 1070
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 3 cut(s) 30, 606, 981
GluI GCNGC 3 cut(s) 30, 606, 981
GsuI CTGGAG 2 cut(s) 296, 1133
HaeIII GGCC 2 cut(s) 596, 923
HapII CCGG 5 cut(s) 243, 453, 609, 819, 1182
Hin1II CATG 6 cut(s) 311, 440, 544, 724, 1106, 1225
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 3 cut(s) 103, 582, 727
HinfI GANTC 5 cut(s) 206, 399, 518, 572, 612
HpaII CCGG 5 cut(s) 243, 453, 609, 819, 1182
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 3 cut(s) 465, 767, 955
Hpy188I TCNGA 8 cut(s) 166, 193, 322, 502, 523, 645, 937, 1207
Hpy188III TCNNGA 4 cut(s) 252, 308, 1055, 1103
Hpy8I GTNNAC 3 cut(s) 465, 767, 955
HpyAV CCTTC 3 cut(s) 30, 595, 749
HpyCH4III ACNGT 2 cut(s) 959, 972
HpyCH4IV ACGT 1 cut(s) 1129
HpyCH4V TGCA 6 cut(s) 365, 370, 536, 548, 580, 657
HpyF10VI GCNNNNNNNGC 2 cut(s) 602, 730
HpyF3I CTNAG 2 cut(s) 89, 846
HpySE526I ACGT 1 cut(s) 1129
Hsp92II CATG 6 cut(s) 311, 440, 544, 724, 1106, 1225
KroI GCCGGC 1 cut(s) 1181
KroNI GCCGGC 1 cut(s) 1183
Kzo9I GATC 4 cut(s) 304, 310, 828, 1168
LguI GCTCTTC 2 cut(s) 488, 1186
Lsp1109I GCAGC 2 cut(s) 16, 592
LweI GCATC 3 cut(s) 153, 523, 805
MaeII ACGT 1 cut(s) 1129
MaeIII GTNAC 1 cut(s) 314
MalI GATC 4 cut(s) 306, 312, 830, 1170
MboI GATC 4 cut(s) 304, 310, 828, 1168
MhlI GDGCHC 3 cut(s) 500, 720, 1097
MluCI AATT 8 cut(s) 136, 170, 345, 670, 890, 909, 963, 1226
MmeI TCCRAC 1 cut(s) 546
Mph1103I ATGCAT 1 cut(s) 538
MroNI GCCGGC 1 cut(s) 1181
MseI TTAA 6 cut(s) 44, 473, 510, 660, 876, 1047
MslI CAYNNNNRTG 1 cut(s) 1056
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 5 cut(s) 243, 453, 609, 819, 1182
MspR9I CCNGG 3 cut(s) 202, 243, 244
Mva1269I GAATGC 3 cut(s) 536, 548, 982
MvaI CCWGG 1 cut(s) 202
MwoI GCNNNNNNNGC 2 cut(s) 602, 730
NaeI GCCGGC 1 cut(s) 1183
NciI CCSGG 2 cut(s) 243, 244
NdeI CATATG 1 cut(s) 529
NdeII GATC 4 cut(s) 304, 310, 828, 1168
NgoMIV GCCGGC 1 cut(s) 1181
NlaIII CATG 6 cut(s) 311, 440, 544, 724, 1106, 1225
NlaIV GGNNCC 1 cut(s) 132
NmeAIII GCCGAG 4 cut(s) 41, 58, 572, 1076
NsiI ATGCAT 1 cut(s) 538
NspI RCATGY 2 cut(s) 440, 724
PagI TCATGA 2 cut(s) 307, 1102
PciI ACATGT 1 cut(s) 436
PciSI GCTCTTC 2 cut(s) 488, 1186
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 3 cut(s) 536, 548, 982
PdiI GCCGGC 1 cut(s) 1183
PfeI GAWTC 5 cut(s) 206, 399, 518, 572, 612
PkrI GCNGC 3 cut(s) 31, 607, 982
PscI ACATGT 1 cut(s) 436
Psp124BI GAGCTC 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 200
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 952
PsrI GAACNNNNNNTAC 2 cut(s) 1123, 1155
RsaI GTAC 4 cut(s) 590, 866, 1010, 1085
RsaNI GTAC 4 cut(s) 589, 865, 1009, 1084
RseI CAYNNNNRTG 1 cut(s) 1056
SacI GAGCTC 1 cut(s) 500
SapI GCTCTTC 2 cut(s) 488, 1186
SaqAI TTAA 6 cut(s) 44, 473, 510, 660, 876, 1047
SatI GCNGC 3 cut(s) 30, 606, 981
Sau3AI GATC 4 cut(s) 304, 310, 828, 1168
Sau96I GGNCC 1 cut(s) 952
ScaI AGTACT 1 cut(s) 1010
ScrFI CCNGG 3 cut(s) 202, 243, 244
SduI GDGCHC 3 cut(s) 500, 720, 1097
SfaNI GCATC 3 cut(s) 153, 523, 805
SinI GGWCC 1 cut(s) 952
SmaI CCCGGG 1 cut(s) 244
SmiMI CAYNNNNRTG 1 cut(s) 1056
SmlI CTYRAG 1 cut(s) 875
SmoI CTYRAG 1 cut(s) 875
Sse9I AATT 8 cut(s) 136, 170, 345, 670, 890, 909, 963, 1226
SsiI CCGC 2 cut(s) 177, 981
SstI GAGCTC 1 cut(s) 500
StyD4I CCNGG 3 cut(s) 200, 241, 242
TaaI ACNGT 2 cut(s) 959, 972
TaiI ACGT 1 cut(s) 1132
TaqI TCGA 2 cut(s) 1065, 1189
TasI AATT 8 cut(s) 136, 170, 345, 670, 890, 909, 963, 1226
TatI WGTACW 2 cut(s) 864, 1008
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 5 cut(s) 206, 399, 518, 572, 612
Tru1I TTAA 6 cut(s) 44, 473, 510, 660, 876, 1047
Tru9I TTAA 6 cut(s) 44, 473, 510, 660, 876, 1047
TscAI CASTG 1 cut(s) 962
TseI GCWGC 2 cut(s) 29, 605
TspDTI ATGAA 7 cut(s) 183, 506, 529, 651, 711, 1161, 1238
TspGWI ACGGA 2 cut(s) 784, 932
TspMI CCCGGG 1 cut(s) 242
TspRI CASTG 1 cut(s) 962
Vha464I CTTAAG 1 cut(s) 875
VpaK11BI GGWCC 1 cut(s) 952
XapI RAATTY 2 cut(s) 170, 345
XceI RCATGY 2 cut(s) 440, 724
XmaI CCCGGG 1 cut(s) 242
ZrmI AGTACT 1 cut(s) 1010
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.