Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010991

Physical Location & Seq

Genomic Coordinates Forward (+)

20449399 .. 20454479

Gene Structure 5081 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1170300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGCGCGAAGCACTGCTATGTGAATCGCCGAGCAACGTCGTTTCCCACCCCAAGAAGAAGAGGTCGGAGGAGGAGAAAGGGGACAGGGAGCTGTCGACGCAGTTGGTTGTAGCACCGGCGGCGGTGGCTACAATCGGCCTGAGCCGATCTCAGGCGACGACGCGGCGAGTAACGCCGACGACGACGACGACCCTCGCGACTTTCCCCACCACAACGTCGTCCAGCGTCGAGAGGCATCTACCGAATGGGGATCTCTACATCGGGAGCTTCTCCGGGTCGTTCCCGCACGGATCCGGCAAGTACCTCTGGACGGACGGGTGCATGTACGAAGGCGAGTGGCGACGTGGCAAGGCGTCGGGGAAAGGCAAGTTCTCGTGGCCGTCGGGCGCAACGTACGAGGGCGAGTTCAAGTCGGGTCGGATGGAGGGGTGCGGGACGTTCACCGGATCCGACGGGGATACATATCGGGGCTCCTGGTCGTCGGATCGGAAACACGGGTTGGGGGAGAAGCGGTACGCGAACGGCGATTTCTACGAGGGTTCGTGGAAGCGAAACGTCCAGGACGGGCAAGGGCGGTACGTGTGGGAGAACGGCAATGAGTACGTCGGCGAGTGGCGAAATGGCGTCATTTCGGGTCGGGGCGTTTTGATTTGGGCCAACGGGAACAAATACGACGGCCAGTGGGAAAACGGCGTTCCCAAAGGTAACGGAATATTCACCTGGCCCGACGGCAGTTGTTATGTTGGGACATGGAACAAGGACTTGAGGTATCAGCTTAACGGGACTTTCTATCCGGCGAACGGCAGAAAGGAGCAGAGCTTGACCGACATTGGGGCTTTCGGAGCGGAGAATCTGACCATTACGATGCGAAAACGCAGCGTTTCGTCGGTGGATGGGGCGAGAGGGAGCTTGGCGGAGAGGACTTTCCCCAGGATTTGTATCTGGGAGTCGGATGGGGAAGCCGGGGATATCACATGCGATATCATCGACAACATGGAGGCTTCGATGTTCTATCGAAACGGCACCGCATTCGATCGAGATGGGTTCGCGAAGTTCCGGCGGAGTCCTTGTTGTTTCTCCGGCGAGCCCAAGAAGCCCGGCCAGACGATTTCCAAAGGGCATAAGAATTACGATTTGATGCTCAATCTTCAGCTGGGCATTAGGTACTCAATTGGGAAGCATGCTTCGATTGTGAGGGACCTTAAGCCGAGTGATTTCGATCCAAAGGAGAAGTTCTGGACCAGGTTTCCACCCGAAGGATCGAACAAAACGCCACCCCATCAGTCGTTTGAGTTCCGGTGGAAGGACTATTGTCCCATGGTGTTCAGACGTTTGAGGGAGCTATTCCAAGTAGATCCAGCCGATTATATGCTGGCTATCTGCGGAAATGACGCGCTTAGGGAGCTTTCTTCTCCAGGGAAGAGCGGAAGCTTCTTTTACCTGACTCAGGACGATAGATTTATGATTAAGACTGTCAAGAAATCGGAAGTCAAGGTGCTTATCAGGATGCTTCCAAGTTACTACAGACATATGTCTCGGTATGAAAATTCTCTGGTGACAAAATTCTATGGCGTGCATTGTGTCAAACCTGTCGGTGGCCAGAAGACAAGGTTTATCGTGATGGGCAATTTGTTCTGCTCAGAGTACAGAATACATAGACGATTTGACCTTAAAGGATCCTCCCATGGTCGAACAACTGATAAACCTGAGGGGGAGATTGATGAAATCACAACTCTCAAGGATCTTGACCTTAACTTTGTGTTTCGCCTCCAAGGAAAATGGTACCATGAGCTTATGAAGCAAATTGATCTGGATTGTGAATTTCTGGAAGCAGAGAGAATCATGGATTACAGTCTTTTGGTTGGACTTCATTTCTACGATGGTACGACATATGACAAAATGGGGCTTTCACCATTTTATTTGCGCTCTGGACAAAAGGATTCATATCAAAATGAGAAGTTTATGCGTGGGTGTCGATTTATTGAGGCTGAGCTACAAGACATGGATCGAGTTTTGTCTGGCCGGAAACCATTGATCTGTTTAGGAGCCAATATGCCTGCAAGGGCAGAGCAGATGGCTCGAAGGAGTGACTTCGATCAGTACACCCCTGGTGGGTTCAGCCATTTGACCCCTTCACGCAGCGGTGAAATCTACGACGTAGTCCTTTACTTTGGGGTCATCGACATTTTACAAGACTACGATATCAGCAAGAAGTTAGAGCATGCATATAAATCATTGCAAGCCGACCCAACTTCGATCTCAGCTGTTGATCCGAAGCATTACTCAAAAAGGTTCAGGGATTTCATCGGAAGAATTTTCGTAGAAGACAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0003785 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007275 GO:0008092 GO:0008150 GO:0008152 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009791 GO:0009958 GO:0009987 GO:0010015 GO:0010101 GO:0010102 GO:0010311 GO:0016020 GO:0016301 GO:0016307 GO:0016308 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0022414 GO:0022622 GO:0030258 GO:0032501 GO:0032502 GO:0040007 GO:0044237 GO:0044238 GO:0044255 GO:0044464 GO:0044877 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048364 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048856 GO:0050896 GO:0051015 GO:0071704 GO:0071944 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04011 ko04070 ko04072 ko04139 ko04144 ko04666 ko04810 ko05231 map00562 map01100 map04011 map04070 map04072 map04139 map04144 map04666 map04810 map05231

Pfam Domains

Protein Families

MORN PIP5K

Protein Analysis

778

Amino Acids

88.46

Weight (kDa)

8.75

Isoelectric Point (pI)

40.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MORN	PF02493	109 - 130	2.3e-07	MORN repeat
MORN	PF02493	132 - 154	9.1e-06	MORN repeat
MORN	PF02493	201 - 222	1.1e-06	MORN repeat
MORN	PF02493	224 - 245	5.1e-07	MORN repeat
PIP5K	PF01504	460 - 771	4.8e-102	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21980 AT1G77740 AT1G77740
fragaria_vesca	FvH4_2g26240 FvH4_2g26240
malus_domestica	MD08G1170300.v1.1 MD15G1357100.v1.1
prunus_persica	Prupe.1G504100_v2.0.a1
pyrus_communis	pycom08g14580 pycom15g31930
rosa_chinensis	RchiOBHm_Chr6g0294851
rosa_laevigata	RLG00000011837
rosa_multiflora	Rmu_sc0008966.1_g000020 Rmu_sc0029501.1_g000001
rosa_roxburghii	Rroxscaffold_7G00172980
rosa_rugosa	Rorug06G0247100
rosa_samantha	Rh6AG360700 Rh6BG367400 Rh6CG374500
rosa_wichuraiana	Rw6G031470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1782
AccB1I	GGYRCC	2 cut(s)	1022, 1782
AccBSI	CCGCTC	2 cut(s)	845, 1425
AccI	GTMKAC	1 cut(s)	95
AccII	CGCG	6 cut(s)	6, 163, 197, 518, 1049, 1394
AcoI	YGGCCR	5 cut(s)	377, 676, 1099, 1597, 2020
AcsI	RAATTY	4 cut(s)	1546, 1562, 1820, 2313
AcuI	CTGAAG	1 cut(s)	1133
AcyI	GRCGYC	2 cut(s)	353, 624
AfiI	CCNNNNNNNGG	9 cut(s)	151, 310, 800, 831, 1256, 1303, 1447, 1595, 2112
AflII	CTTAAG	1 cut(s)	1202
AflIII	ACRYGT	1 cut(s)	579
AgsI	TTSAA	1 cut(s)	409
AjiI	CACGTC	1 cut(s)	344
AjnI	CCWGG	7 cut(s)	473, 558, 719, 929, 1241, 1414, 2107
AjuI	GAANNNNNNNTTGG	2 cut(s)	482, 514
Alw26I	GTCTC	1 cut(s)	1539
AoxI	GGCC	8 cut(s)	136, 377, 654, 676, 722, 1099, 1597, 2020
ApeKI	GCWGC	2 cut(s)	876, 2139
ApoI	RAATTY	4 cut(s)	1546, 1562, 1820, 2313
ArsI	GACNNNNNNTTYG	2 cut(s)	1739, 1771
Asp718I	GGTACC	1 cut(s)	1782
AspLEI	GCGC	4 cut(s)	6, 389, 1396, 1926
AspS9I	GGNCC	4 cut(s)	654, 723, 1198, 1239
AsuC2I	CCSGG	3 cut(s)	274, 964, 1098
AsuHPI	GGTGA	5 cut(s)	433, 709, 1567, 1902, 2156
AvaII	GGWCC	2 cut(s)	1198, 1239
AxyI	CCTNAGG	1 cut(s)	1707
BalI	TGGCCA	1 cut(s)	1599
BamHI	GGATCC	3 cut(s)	290, 446, 1676
BanI	GGYRCC	2 cut(s)	1022, 1782
BanII	GRGCYC	2 cut(s)	473, 1089
BauI	CACGAG	1 cut(s)	373
BbsI	GAAGAC	1 cut(s)	1610
BbvI	GCAGC	2 cut(s)	888, 2151
BccI	CCATC	8 cut(s)	415, 887, 947, 1034, 1287, 1615, 1874, 2068
BceAI	ACGGC	8 cut(s)	364, 538, 607, 691, 706, 745, 817, 1036
BcgI	CGANNNNNNTGC	4 cut(s)	1012, 1046, 2060, 2094
BciT130I	CCWGG	7 cut(s)	475, 560, 721, 931, 1243, 1416, 2109
BciVI	GTATCC	1 cut(s)	451
BcnI	CCSGG	3 cut(s)	274, 964, 1098
BcoDI	GTCTC	1 cut(s)	1539
BfmI	CTRYAG	1 cut(s)	1522
BfrI	CTTAAG	1 cut(s)	1202
BfuI	GTATCC	1 cut(s)	451
BisI	GCNGC	4 cut(s)	120, 164, 877, 2140
BlpI	GCTNAGC	1 cut(s)	1989
BlsI	GCNGC	4 cut(s)	121, 165, 878, 2141
Bme18I	GGWCC	2 cut(s)	1198, 1239
BmgBI	CACGTC	1 cut(s)	344
BmgT120I	GGNCC	4 cut(s)	654, 723, 1198, 1239
BmiI	GGNNCC	8 cut(s)	292, 448, 472, 1024, 1199, 1678, 1784, 2047
BmsI	GCATC	4 cut(s)	244, 855, 1128, 1497
BoxI	GACNNNNGTC	2 cut(s)	1311, 1531
BpiI	GAAGAC	1 cut(s)	1610
BplI	GAGNNNNNCTC	2 cut(s)	133, 165
BpmI	CTGGAG	1 cut(s)	1398
Bpu10I	CCTNAGC	2 cut(s)	140, 1397
Bpu1102I	GCTNAGC	1 cut(s)	1989
BpuEI	CTTGAG	2 cut(s)	784, 1721
BpuMI	CCSGG	3 cut(s)	274, 964, 1098
BsaAI	YACGTR	1 cut(s)	580
BsaBI	GATNNNNATC	4 cut(s)	462, 938, 1218, 1944
BsaHI	GRCGYC	2 cut(s)	353, 624
BsaJI	CCNNGG	7 cut(s)	929, 963, 1317, 1415, 1684, 1771, 2107
BsaWI	WCCGGW	2 cut(s)	443, 1296
BsaXI	ACNNNNNCTCC	4 cut(s)	497, 527, 1054, 1084
Bsc4I	CCNNNNNNNGG	9 cut(s)	151, 310, 800, 831, 1256, 1303, 1447, 1595, 2112
Bse118I	RCCGGY	1 cut(s)	115
Bse1I	ACTGG	1 cut(s)	679
Bse21I	CCTNAGG	1 cut(s)	1707
Bse3DI	GCAATG	2 cut(s)	601, 2234
Bse8I	GATNNNNATC	4 cut(s)	462, 938, 1218, 1944
BseBI	CCWGG	7 cut(s)	475, 560, 721, 931, 1243, 1416, 2109
BseDI	CCNNGG	7 cut(s)	929, 963, 1317, 1415, 1684, 1771, 2107
BseGI	GGATG	4 cut(s)	426, 898, 958, 1512
BseJI	GATNNNNATC	4 cut(s)	462, 938, 1218, 1944
BseLI	CCNNNNNNNGG	9 cut(s)	151, 310, 800, 831, 1256, 1303, 1447, 1595, 2112
BseMI	GCAATG	2 cut(s)	601, 2234
BseMII	CTCAG	7 cut(s)	131, 164, 1460, 1653, 1698, 1980, 2274
BseNI	ACTGG	1 cut(s)	679
BseRI	GAGGAG	2 cut(s)	83, 86
BseXI	GCAGC	2 cut(s)	888, 2151
BseYI	CCCAGC	1 cut(s)	1153
Bsh1236I	CGCG	6 cut(s)	6, 163, 197, 518, 1049, 1394
Bsh1285I	CGRYCG	1 cut(s)	1036
BshFI	GGCC	8 cut(s)	138, 379, 656, 678, 724, 1101, 1599, 2022
BshNI	GGYRCC	2 cut(s)	1022, 1782
BsiEI	CGRYCG	1 cut(s)	1036
BsiWI	CGTACG	1 cut(s)	393
BslFI	GGGAC	6 cut(s)	95, 448, 760, 796, 1211, 1299
BslI	CCNNNNNNNGG	9 cut(s)	151, 310, 800, 831, 1256, 1303, 1447, 1595, 2112
BsmAI	GTCTC	1 cut(s)	1539
BsmFI	GGGAC	6 cut(s)	95, 448, 760, 796, 1211, 1299
BsmI	GAATGC	1 cut(s)	1028
BsnI	GGCC	8 cut(s)	138, 379, 656, 678, 724, 1101, 1599, 2022
Bsp1286I	GDGCHC	2 cut(s)	473, 1089
Bsp1720I	GCTNAGC	1 cut(s)	1989
Bsp19I	CCATGG	2 cut(s)	1317, 1684
Bsp68I	TCGCGA	2 cut(s)	197, 1049
BspANI	GGCC	8 cut(s)	138, 379, 656, 678, 724, 1101, 1599, 2022
BspCNI	CTCAG	7 cut(s)	132, 163, 1459, 1652, 1699, 1981, 2273
BspFNI	CGCG	6 cut(s)	6, 163, 197, 518, 1049, 1394
BspLI	GGNNCC	8 cut(s)	292, 448, 472, 1024, 1199, 1678, 1784, 2047
BspQI	GCTCTTC	1 cut(s)	1415
BspT107I	GGYRCC	2 cut(s)	1022, 1782
BspTI	CTTAAG	1 cut(s)	1202
BsrBI	CCGCTC	2 cut(s)	845, 1425
BsrDI	GCAATG	2 cut(s)	601, 2234
BsrFI	RCCGGY	1 cut(s)	115
BsrI	ACTGG	1 cut(s)	679
BssAI	RCCGGY	1 cut(s)	115
BssECI	CCNNGG	7 cut(s)	929, 963, 1317, 1415, 1684, 1771, 2107
BssNI	GRCGYC	2 cut(s)	353, 624
BssSI	CACGAG	1 cut(s)	373
BssT1I	CCWWGG	3 cut(s)	1317, 1684, 1771
Bst2BI	CACGAG	1 cut(s)	373
Bst2UI	CCWGG	7 cut(s)	475, 560, 721, 931, 1243, 1416, 2109
Bst4CI	ACNGT	2 cut(s)	1474, 1853
Bst6I	CTCTTC	2 cut(s)	53, 1415
BstACI	GRCGYC	2 cut(s)	353, 624
BstAFI	CTTAAG	1 cut(s)	1202
BstBAI	YACGTR	1 cut(s)	580
BstC8I	GCNNGC	7 cut(s)	1085, 1182, 1374, 1574, 2058, 2223, 2241
BstDEI	CTNAG	8 cut(s)	140, 150, 1397, 1446, 1639, 1707, 1989, 2260
BstDSI	CCRYGG	2 cut(s)	1317, 1684
BstENI	CCTNNNNNAGG	1 cut(s)	1445
BstF5I	GGATG	4 cut(s)	426, 898, 958, 1512
BstFNI	CGCG	6 cut(s)	6, 163, 197, 518, 1049, 1394
BstHHI	GCGC	4 cut(s)	6, 389, 1396, 1926
BstMAI	GTCTC	1 cut(s)	1539
BstMCI	CGRYCG	1 cut(s)	1036
BstMWI	GCNNNNNNNGC	8 cut(s)	97, 119, 125, 172, 842, 1093, 1402, 1798
BstNI	CCWGG	7 cut(s)	475, 560, 721, 931, 1243, 1416, 2109
BstNSI	RCATGY	4 cut(s)	325, 978, 1184, 2225
BstPAI	GACNNNNGTC	2 cut(s)	1311, 1531
BstSFI	CTRYAG	1 cut(s)	1522
BstUI	CGCG	6 cut(s)	6, 163, 197, 518, 1049, 1394
BstV1I	GCAGC	2 cut(s)	888, 2151
BstV2I	GAAGAC	1 cut(s)	1610
BstX2I	RGATCY	6 cut(s)	250, 290, 446, 1354, 1676, 1741
BstYI	RGATCY	6 cut(s)	250, 290, 446, 1354, 1676, 1741
Bsu36I	CCTNAGG	1 cut(s)	1707
BsuI	GTATCC	1 cut(s)	451
BsuRI	GGCC	8 cut(s)	138, 379, 656, 678, 724, 1101, 1599, 2022
BtgI	CCRYGG	2 cut(s)	1317, 1684
BtrI	CACGTC	1 cut(s)	344
BtsCI	GGATG	4 cut(s)	426, 898, 958, 1512
BtsI	GCAGTG	1 cut(s)	11
BtsIMutI	CAGTG	2 cut(s)	11, 686
BtuMI	TCGCGA	2 cut(s)	197, 1049
Cac8I	GCNNGC	7 cut(s)	1085, 1182, 1374, 1574, 2058, 2223, 2241
CfoI	GCGC	4 cut(s)	6, 389, 1396, 1926
Cfr10I	RCCGGY	1 cut(s)	115
Cfr13I	GGNCC	4 cut(s)	654, 723, 1198, 1239
CseI	GACGC	6 cut(s)	106, 169, 214, 342, 613, 1400
CsiI	ACCWGGT	1 cut(s)	1241
DdeI	CTNAG	8 cut(s)	140, 150, 1397, 1446, 1639, 1707, 1989, 2260
EaeI	YGGCCR	5 cut(s)	377, 676, 1099, 1597, 2020
Eam1104I	CTCTTC	2 cut(s)	53, 1415
EarI	CTCTTC	2 cut(s)	53, 1415
EciI	GGCGGA	2 cut(s)	929, 1075
Eco130I	CCWWGG	3 cut(s)	1317, 1684, 1771
Eco24I	GRGCYC	2 cut(s)	473, 1089
Eco32I	GATATC	3 cut(s)	970, 982, 2203
Eco47I	GGWCC	2 cut(s)	1198, 1239
Eco57I	CTGAAG	1 cut(s)	1133
Eco81I	CCTNAGG	1 cut(s)	1707
EcoNI	CCTNNNNNAGG	1 cut(s)	1445
EcoO109I	RGGNCCY	1 cut(s)	1198
EcoRII	CCWGG	7 cut(s)	473, 558, 719, 929, 1241, 1414, 2107
EcoRV	GATATC	3 cut(s)	970, 982, 2203
EcoT14I	CCWWGG	3 cut(s)	1317, 1684, 1771
EcoT22I	ATGCAT	1 cut(s)	2227
EcoT38I	GRGCYC	2 cut(s)	473, 1089
ErhI	CCWWGG	3 cut(s)	1317, 1684, 1771
FaqI	GGGAC	6 cut(s)	95, 448, 760, 796, 1211, 1299
FauI	CCCGC	2 cut(s)	291, 425
FauNDI	CATATG	2 cut(s)	1530, 1891
FblI	GTMKAC	1 cut(s)	95
Fnu4HI	GCNGC	4 cut(s)	120, 164, 877, 2140
FokI	GGATG	4 cut(s)	433, 905, 965, 1519
FriOI	GRGCYC	2 cut(s)	473, 1089
Fsp4HI	GCNGC	4 cut(s)	120, 164, 877, 2140
GlaI	GCGC	4 cut(s)	5, 388, 1395, 1925
GluI	GCNGC	4 cut(s)	120, 164, 877, 2140
GsaI	CCCAGC	1 cut(s)	1157
GsuI	CTGGAG	1 cut(s)	1398
HaeIII	GGCC	8 cut(s)	138, 379, 656, 678, 724, 1101, 1599, 2022
HgaI	GACGC	6 cut(s)	106, 169, 214, 342, 613, 1400
HhaI	GCGC	4 cut(s)	6, 389, 1396, 1926
Hin1I	GRCGYC	2 cut(s)	353, 624
Hin6I	GCGC	4 cut(s)	4, 387, 1394, 1924
HinP1I	GCGC	4 cut(s)	4, 387, 1394, 1924
HincII	GTYRAC	1 cut(s)	96
HindII	GTYRAC	1 cut(s)	96
HindIII	AAGCTT	1 cut(s)	1429
HinfI	GANTC	7 cut(s)	23, 850, 947, 1063, 1444, 1839, 1940
HphI	GGTGA	5 cut(s)	433, 709, 1567, 1902, 2156
Hpy166II	GTNNAC	3 cut(s)	96, 441, 2103
Hpy8I	GTNNAC	3 cut(s)	96, 441, 2103
HpyAV	CCTTC	5 cut(s)	323, 1250, 1297, 2076, 2142
HpyCH4III	ACNGT	2 cut(s)	1474, 1853
HpyCH4V	TGCA	5 cut(s)	321, 1576, 2060, 2225, 2239
HpyF10VI	GCNNNNNNNGC	8 cut(s)	97, 119, 125, 172, 842, 1093, 1402, 1798
HpyF3I	CTNAG	8 cut(s)	140, 150, 1397, 1446, 1639, 1707, 1989, 2260
Hsp92I	GRCGYC	2 cut(s)	353, 624
HspAI	GCGC	4 cut(s)	4, 387, 1394, 1924
KpnI	GGTACC	1 cut(s)	1786
LguI	GCTCTTC	1 cut(s)	1415
LmnI	GCTCC	9 cut(s)	88, 264, 476, 811, 842, 906, 1339, 1402, 2045
Lsp1109I	GCAGC	2 cut(s)	888, 2151
LweI	GCATC	4 cut(s)	244, 855, 1128, 1497
MabI	ACCWGGT	1 cut(s)	1241
MaeIII	GTNAC	5 cut(s)	169, 704, 1517, 1555, 2087
MbiI	CCGCTC	2 cut(s)	845, 1425
MboII	GAAGA	7 cut(s)	67, 70, 1139, 1401, 1432, 1615, 2322
MfeI	CAATTG	1 cut(s)	1170
MflI	RGATCY	6 cut(s)	250, 290, 446, 1354, 1676, 1741
MhlI	GDGCHC	2 cut(s)	473, 1089
MlsI	TGGCCA	1 cut(s)	1599
MluCI	AATT	8 cut(s)	1126, 1170, 1546, 1562, 1627, 1803, 1820, 2313
MluNI	TGGCCA	1 cut(s)	1599
MlyI	GAGTC	3 cut(s)	956, 1072, 1438
MmeI	TCCRAC	6 cut(s)	45, 398, 462, 474, 930, 1843
Mox20I	TGGCCA	1 cut(s)	1599
Mph1103I	ATGCAT	1 cut(s)	2227
MscI	TGGCCA	1 cut(s)	1599
MseI	TTAA	5 cut(s)	777, 1203, 1467, 1671, 1752
MslI	CAYNNNNRTG	2 cut(s)	16, 863
Msp20I	TGGCCA	1 cut(s)	1599
MspA1I	CMGCKG	3 cut(s)	1153, 2142, 2264
MspCI	CTTAAG	1 cut(s)	1202
MunI	CAATTG	1 cut(s)	1170
Mva1269I	GAATGC	1 cut(s)	1028
MvaI	CCWGG	7 cut(s)	475, 560, 721, 931, 1243, 1416, 2109
MvnI	CGCG	6 cut(s)	6, 163, 197, 518, 1049, 1394
MwoI	GCNNNNNNNGC	8 cut(s)	97, 119, 125, 172, 842, 1093, 1402, 1798
NciI	CCSGG	3 cut(s)	274, 964, 1098
NcoI	CCATGG	2 cut(s)	1317, 1684
NdeI	CATATG	2 cut(s)	1530, 1891
NlaIV	GGNNCC	8 cut(s)	292, 448, 472, 1024, 1199, 1678, 1784, 2047
NmeAIII	GCCGAG	2 cut(s)	54, 1233
NmuCI	GTSAC	2 cut(s)	1555, 2087
NruI	TCGCGA	2 cut(s)	197, 1049
NsiI	ATGCAT	1 cut(s)	2227
NspI	RCATGY	4 cut(s)	325, 978, 1184, 2225
PaeI	GCATGC	2 cut(s)	1184, 2225
PciSI	GCTCTTC	1 cut(s)	1415
PcsI	WCGNNNNNNNCGW	5 cut(s)	179, 185, 194, 389, 522
PctI	GAATGC	1 cut(s)	1028
PfeI	GAWTC	4 cut(s)	23, 850, 1839, 1940
Pfl23II	CGTACG	1 cut(s)	393
PflFI	GACNNNGTC	1 cut(s)	2159
PfoI	TCCNGGA	1 cut(s)	558
PkrI	GCNGC	4 cut(s)	121, 165, 878, 2141
Ple19I	CGATCG	1 cut(s)	1036
PleI	GAGTC	3 cut(s)	955, 1071, 1438
PpsI	GAGTC	3 cut(s)	955, 1071, 1438
Ppu21I	YACGTR	1 cut(s)	580
PpuMI	RGGWCCY	1 cut(s)	1198
PshAI	GACNNNNGTC	2 cut(s)	1311, 1531
Psp5II	RGGWCCY	1 cut(s)	1198
Psp6I	CCWGG	7 cut(s)	473, 558, 719, 929, 1241, 1414, 2107
PspFI	CCCAGC	1 cut(s)	1153
PspGI	CCWGG	7 cut(s)	473, 558, 719, 929, 1241, 1414, 2107
PspLI	CGTACG	1 cut(s)	393
PspN4I	GGNNCC	8 cut(s)	292, 448, 472, 1024, 1199, 1678, 1784, 2047
PspPI	GGNCC	4 cut(s)	654, 723, 1198, 1239
PspPPI	RGGWCCY	1 cut(s)	1198
PsuI	RGATCY	6 cut(s)	250, 290, 446, 1354, 1676, 1741
PsyI	GACNNNGTC	1 cut(s)	2159
PvuI	CGATCG	1 cut(s)	1036
PvuII	CAGCTG	2 cut(s)	1153, 2264
RruI	TCGCGA	2 cut(s)	197, 1049
RseI	CAYNNNNRTG	2 cut(s)	16, 863
SalI	GTCGAC	1 cut(s)	94
SapI	GCTCTTC	1 cut(s)	1415
SaqAI	TTAA	5 cut(s)	777, 1203, 1467, 1671, 1752
SatI	GCNGC	4 cut(s)	120, 164, 877, 2140
Sau96I	GGNCC	4 cut(s)	654, 723, 1198, 1239
SchI	GAGTC	3 cut(s)	956, 1072, 1438
SduI	GDGCHC	2 cut(s)	473, 1089
SexAI	ACCWGGT	1 cut(s)	1241
SfaNI	GCATC	4 cut(s)	244, 855, 1128, 1497
SfcI	CTRYAG	1 cut(s)	1522
SgrAI	CRCCGGYG	1 cut(s)	115
SinI	GGWCC	2 cut(s)	1198, 1239
SmiMI	CAYNNNNRTG	2 cut(s)	16, 863
SmlI	CTYRAG	3 cut(s)	763, 1202, 1736
SmoI	CTYRAG	3 cut(s)	763, 1202, 1736
SphI	GCATGC	2 cut(s)	1184, 2225
Sse9I	AATT	8 cut(s)	1126, 1170, 1546, 1562, 1627, 1803, 1820, 2313
SspI	AATATT	1 cut(s)	714
StyI	CCWWGG	3 cut(s)	1317, 1684, 1771
TaaI	ACNGT	2 cut(s)	1474, 1853
TaqII	GACCGA	1 cut(s)	839
TasI	AATT	8 cut(s)	1126, 1170, 1546, 1562, 1627, 1803, 1820, 2313
TatI	WGTACW	2 cut(s)	1644, 2100
TauI	GCSGC	2 cut(s)	122, 166
TfiI	GAWTC	4 cut(s)	23, 850, 1839, 1940
Tru1I	TTAA	5 cut(s)	777, 1203, 1467, 1671, 1752
Tru9I	TTAA	5 cut(s)	777, 1203, 1467, 1671, 1752
TscAI	CASTG	2 cut(s)	18, 686
TseFI	GTSAC	2 cut(s)	1555, 2087
TseI	GCWGC	2 cut(s)	876, 2139
Tsp45I	GTSAC	2 cut(s)	1555, 2087
TspDTI	ATGAA	6 cut(s)	1557, 1737, 1811, 1859, 1932, 2293
TspGWI	ACGGA	3 cut(s)	303, 326, 723
TspRI	CASTG	2 cut(s)	18, 686
Tth111I	GACNNNGTC	1 cut(s)	2159
Vha464I	CTTAAG	1 cut(s)	1202
VpaK11BI	GGWCC	2 cut(s)	1198, 1239
XagI	CCTNNNNNAGG	1 cut(s)	1445
XapI	RAATTY	4 cut(s)	1546, 1562, 1820, 2313
XceI	RCATGY	4 cut(s)	325, 978, 1184, 2225
XmiI	GTMKAC	1 cut(s)	95
Zsp2I	ATGCAT	1 cut(s)	2227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.