Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0022337

Physical Location & Seq

Genomic Coordinates Forward (+)

21101613 .. 21105629

Gene Structure 4017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1173500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 750 bp

ATGTGTGACATGGCTCATATCAGCGGTCTTACGGCAGCTAAGGGATGTGCAAGTCCTTTTGATTACTGTGATATTGTTACGTCAACAACACACAAAAGTCTTCAGGGTCCTAGGGGAGGTATTATTTTTTCTAGGAAAGGCCCAAAATTAGGGAAGCAAGGCAAACATCATACTAATGGGGATGGCAATAACCATTACGATTTTGAGGAAAAGATAAACTTTGCCATTTTCCCATCACTACAAGGAGGGCCACACAACAACCATATTGCTGCTCTTGCCATAGCCTTAAAACAAGCAGCTACTCCAGAATATAAAGCATATATGCAACAGGTGAAGAAGAATGCCCAGGCCTTAGCTAATGCTCTGTTAAGAAGAAAATGCAGATTGGTTACTGGGGGCACCGACAATCACTTGTTGCTTTGGGATTTGACTGCTCTTGGTTTAACAGGAATTACGAAAAGGTCTGTGAGATGTGCCACATATATATTTGGTGATAATGGTGCTTTTTCTCCTGGAGGAGTGAGAATCGGAACTCCTGCTATGAGTACACGAGGTTGCGTGGAGTCGGATTTTGAAACAATTGCAGACTTTCTTCTCAGGGCTGCTCAGATCACTGTTTCCATACAGAGGGAATATGGAAAATTCCAGAAAGATTTTATCAAAGATCTCAAGAACAGCAAAGATATTTACGAGCTCAGAAATCGGGTCGAAACATTTGCATCCCAGTTTGAAATGCCAGGATATGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0006730 GO:0006732 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019219 GO:0019222 GO:0031323 GO:0031974 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046500 GO:0046686 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051052 GO:0051171 GO:0051186 GO:0055063 GO:0055081 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072505 GO:0080090 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

250

Amino Acids

27.35

Weight (kDa)

9.18

Isoelectric Point (pI)

39.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	1 - 197	7.6e-66	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022337)

Species	Orthologous Gene IDs
malus_domestica	MD08G1173500.v1.1 MD15G1360000.v1.1
pyrus_communis	pycom04g12090
rosa_samantha	Rh7DG474800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	398
AciI	CCGC	1 cut(s)	24
AcsI	RAATTY	1 cut(s)	641
AcuI	CTGAAG	1 cut(s)	86
AfaI	GTAC	1 cut(s)	547
AfiI	CCNNNNNNNGG	3 cut(s)	116, 149, 627
AgsI	TTSAA	2 cut(s)	575, 731
AjnI	CCWGG	3 cut(s)	345, 511, 736
AluBI	AGCT	4 cut(s)	38, 299, 356, 694
AluI	AGCT	4 cut(s)	38, 299, 356, 694
Alw21I	GWGCWC	1 cut(s)	696
AoxI	GGCC	3 cut(s)	139, 248, 348
ApeKI	GCWGC	4 cut(s)	35, 269, 296, 602
ApoI	RAATTY	1 cut(s)	641
AspA2I	CCTAGG	1 cut(s)	110
AspS9I	GGNCC	3 cut(s)	107, 140, 248
AsuHPI	GGTGA	2 cut(s)	343, 503
AvaII	GGWCC	1 cut(s)	107
AvrII	CCTAGG	1 cut(s)	110
BaeGI	GKGCMC	1 cut(s)	401
BanI	GGYRCC	1 cut(s)	398
BanII	GRGCYC	1 cut(s)	696
BauI	CACGAG	1 cut(s)	549
BbsI	GAAGAC	1 cut(s)	92
Bbv12I	GWGCWC	1 cut(s)	696
BbvI	GCAGC	4 cut(s)	47, 256, 308, 589
BccI	CCATC	2 cut(s)	176, 241
BceAI	ACGGC	1 cut(s)	48
BcgI	CGANNNNNNTGC	2 cut(s)	698, 732
BciT130I	CCWGG	3 cut(s)	347, 513, 738
BfaI	CTAG	2 cut(s)	111, 132
BglII	AGATCT	1 cut(s)	664
BisI	GCNGC	4 cut(s)	36, 270, 297, 603
BlnI	CCTAGG	1 cut(s)	110
BlsI	GCNGC	4 cut(s)	37, 271, 298, 604
Bme1390I	CCNGG	3 cut(s)	347, 513, 738
Bme18I	GGWCC	1 cut(s)	107
BmgT120I	GGNCC	3 cut(s)	107, 140, 248
BmiI	GGNNCC	2 cut(s)	108, 400
BmrFI	CCNGG	3 cut(s)	347, 513, 738
BmrI	ACTGGG	2 cut(s)	402, 718
BmsI	GCATC	1 cut(s)	728
BmuI	ACTGGG	2 cut(s)	402, 718
BpiI	GAAGAC	1 cut(s)	92
BpmI	CTGGAG	2 cut(s)	288, 534
Bpu10I	CCTNAGC	2 cut(s)	39, 352
BpuEI	CTTGAG	1 cut(s)	653
BsaJI	CCNNGG	2 cut(s)	110, 345
Bsc4I	CCNNNNNNNGG	3 cut(s)	116, 149, 627
Bse1I	ACTGG	2 cut(s)	397, 724
BseBI	CCWGG	3 cut(s)	347, 513, 738
BseDI	CCNNGG	2 cut(s)	110, 345
BseGI	GGATG	3 cut(s)	50, 187, 719
BseLI	CCNNNNNNNGG	3 cut(s)	116, 149, 627
BseMII	CTCAG	3 cut(s)	610, 620, 709
BseNI	ACTGG	2 cut(s)	397, 724
BseRI	GAGGAG	1 cut(s)	531
BseSI	GKGCMC	1 cut(s)	401
BseXI	GCAGC	4 cut(s)	47, 256, 308, 589
BshFI	GGCC	3 cut(s)	141, 250, 350
BshNI	GGYRCC	1 cut(s)	398
BsiHKAI	GWGCWC	1 cut(s)	696
BslI	CCNNNNNNNGG	3 cut(s)	116, 149, 627
BsmI	GAATGC	1 cut(s)	346
BsnI	GGCC	3 cut(s)	141, 250, 350
Bsp1286I	GDGCHC	2 cut(s)	401, 696
Bsp143I	GATC	2 cut(s)	609, 664
BspACI	CCGC	1 cut(s)	24
BspANI	GGCC	3 cut(s)	141, 250, 350
BspCNI	CTCAG	3 cut(s)	609, 619, 708
BspLI	GGNNCC	2 cut(s)	108, 400
BspT107I	GGYRCC	1 cut(s)	398
BsrI	ACTGG	2 cut(s)	397, 724
BssECI	CCNNGG	2 cut(s)	110, 345
BssMI	GATC	2 cut(s)	609, 664
BssSI	CACGAG	1 cut(s)	549
BssT1I	CCWWGG	1 cut(s)	110
Bst2BI	CACGAG	1 cut(s)	549
Bst2UI	CCWGG	3 cut(s)	347, 513, 738
Bst4CI	ACNGT	2 cut(s)	68, 616
BstDEI	CTNAG	5 cut(s)	39, 352, 596, 606, 695
BstENI	CCTNNNNNAGG	1 cut(s)	114
BstF5I	GGATG	3 cut(s)	50, 187, 719
BstKTI	GATC	2 cut(s)	612, 667
BstMBI	GATC	2 cut(s)	609, 664
BstMWI	GCNNNNNNNGC	1 cut(s)	275
BstNI	CCWGG	3 cut(s)	347, 513, 738
BstSCI	CCNGG	3 cut(s)	345, 511, 736
BstSLI	GKGCMC	1 cut(s)	401
BstV1I	GCAGC	4 cut(s)	47, 256, 308, 589
BstV2I	GAAGAC	1 cut(s)	92
BstX2I	RGATCY	1 cut(s)	664
BstYI	RGATCY	1 cut(s)	664
BsuRI	GGCC	3 cut(s)	141, 250, 350
BtsCI	GGATG	3 cut(s)	50, 187, 719
BtsIMutI	CAGTG	1 cut(s)	612
Cfr13I	GGNCC	3 cut(s)	107, 140, 248
Csp6I	GTAC	1 cut(s)	546
CviAII	CATG	1 cut(s)	10
CviQI	GTAC	1 cut(s)	546
DdeI	CTNAG	5 cut(s)	39, 352, 596, 606, 695
DpnI	GATC	2 cut(s)	611, 666
DpnII	GATC	2 cut(s)	609, 664
Ecl136II	GAGCTC	1 cut(s)	694
Eco130I	CCWWGG	1 cut(s)	110
Eco147I	AGGCCT	1 cut(s)	350
Eco24I	GRGCYC	1 cut(s)	696
Eco47I	GGWCC	1 cut(s)	107
Eco53kI	GAGCTC	1 cut(s)	694
Eco57I	CTGAAG	1 cut(s)	86
EcoICRI	GAGCTC	1 cut(s)	694
EcoNI	CCTNNNNNAGG	1 cut(s)	114
EcoO109I	RGGNCCY	1 cut(s)	107
EcoRII	CCWGG	3 cut(s)	345, 511, 736
EcoT14I	CCWWGG	1 cut(s)	110
EcoT38I	GRGCYC	1 cut(s)	696
ErhI	CCWWGG	1 cut(s)	110
FaeI	CATG	1 cut(s)	13
FalI	AAGNNNNNCTT	2 cut(s)	203, 235
FatI	CATG	1 cut(s)	9
Fnu4HI	GCNGC	4 cut(s)	36, 270, 297, 603
FokI	GGATG	3 cut(s)	57, 194, 706
FriOI	GRGCYC	1 cut(s)	696
Fsp4HI	GCNGC	4 cut(s)	36, 270, 297, 603
FspBI	CTAG	2 cut(s)	111, 132
GluI	GCNGC	4 cut(s)	36, 270, 297, 603
GsuI	CTGGAG	2 cut(s)	288, 534
HaeIII	GGCC	3 cut(s)	141, 250, 350
Hin1II	CATG	1 cut(s)	13
HincII	GTYRAC	1 cut(s)	84
HindII	GTYRAC	1 cut(s)	84
HinfI	GANTC	2 cut(s)	525, 563
HphI	GGTGA	2 cut(s)	343, 503
Hpy166II	GTNNAC	2 cut(s)	84, 548
Hpy188I	TCNGA	4 cut(s)	530, 568, 609, 698
Hpy188III	TCNNGA	3 cut(s)	305, 646, 670
Hpy8I	GTNNAC	2 cut(s)	84, 548
HpyCH4III	ACNGT	2 cut(s)	68, 616
HpyCH4IV	ACGT	1 cut(s)	80
HpyCH4V	TGCA	5 cut(s)	50, 325, 381, 584, 719
HpyF10VI	GCNNNNNNNGC	1 cut(s)	275
HpyF3I	CTNAG	5 cut(s)	39, 352, 596, 606, 695
HpySE526I	ACGT	1 cut(s)	80
Hsp92II	CATG	1 cut(s)	13
Kzo9I	GATC	2 cut(s)	609, 664
Lsp1109I	GCAGC	4 cut(s)	47, 256, 308, 589
LweI	GCATC	1 cut(s)	728
MaeI	CTAG	2 cut(s)	111, 132
MaeII	ACGT	1 cut(s)	80
MaeIII	GTNAC	3 cut(s)	5, 76, 388
MalI	GATC	2 cut(s)	611, 666
MboI	GATC	2 cut(s)	609, 664
MboII	GAAGA	5 cut(s)	92, 346, 349, 384, 584
MfeI	CAATTG	1 cut(s)	579
MflI	RGATCY	1 cut(s)	664
MhlI	GDGCHC	2 cut(s)	401, 696
MluCI	AATT	4 cut(s)	146, 450, 579, 641
MlyI	GAGTC	1 cut(s)	572
MmeI	TCCRAC	1 cut(s)	546
MnlI	CCTC	6 cut(s)	110, 199, 239, 509, 545, 621
MseI	TTAA	3 cut(s)	287, 368, 443
MslI	CAYNNNNRTG	1 cut(s)	174
MspA1I	CMGCKG	1 cut(s)	24
MspR9I	CCNGG	3 cut(s)	347, 513, 738
MunI	CAATTG	1 cut(s)	579
Mva1269I	GAATGC	1 cut(s)	346
MvaI	CCWGG	3 cut(s)	347, 513, 738
MwoI	GCNNNNNNNGC	1 cut(s)	275
NdeII	GATC	2 cut(s)	609, 664
NlaIII	CATG	1 cut(s)	13
NlaIV	GGNNCC	2 cut(s)	108, 400
NmuCI	GTSAC	1 cut(s)	5
PceI	AGGCCT	1 cut(s)	350
PctI	GAATGC	1 cut(s)	346
PfeI	GAWTC	1 cut(s)	525
PfoI	TCCNGGA	1 cut(s)	511
PkrI	GCNGC	4 cut(s)	37, 271, 298, 604
PleI	GAGTC	1 cut(s)	571
PpsI	GAGTC	1 cut(s)	571
PpuMI	RGGWCCY	1 cut(s)	107
Psp124BI	GAGCTC	1 cut(s)	696
Psp5II	RGGWCCY	1 cut(s)	107
Psp6I	CCWGG	3 cut(s)	345, 511, 736
PspGI	CCWGG	3 cut(s)	345, 511, 736
PspN4I	GGNNCC	2 cut(s)	108, 400
PspPI	GGNCC	3 cut(s)	107, 140, 248
PspPPI	RGGWCCY	1 cut(s)	107
PsuI	RGATCY	1 cut(s)	664
RsaI	GTAC	1 cut(s)	547
RsaNI	GTAC	1 cut(s)	546
RseI	CAYNNNNRTG	1 cut(s)	174
SacI	GAGCTC	1 cut(s)	696
SaqAI	TTAA	3 cut(s)	287, 368, 443
SatI	GCNGC	4 cut(s)	36, 270, 297, 603
Sau3AI	GATC	2 cut(s)	609, 664
Sau96I	GGNCC	3 cut(s)	107, 140, 248
SchI	GAGTC	1 cut(s)	572
ScrFI	CCNGG	3 cut(s)	347, 513, 738
SduI	GDGCHC	2 cut(s)	401, 696
SetI	ASST	9 cut(s)	40, 83, 121, 301, 333, 358, 464, 556, 696
SfaNI	GCATC	1 cut(s)	728
SinI	GGWCC	1 cut(s)	107
SmiMI	CAYNNNNRTG	1 cut(s)	174
SmlI	CTYRAG	1 cut(s)	668
SmoI	CTYRAG	1 cut(s)	668
Sse9I	AATT	4 cut(s)	146, 450, 579, 641
SseBI	AGGCCT	1 cut(s)	350
SsiI	CCGC	1 cut(s)	24
SspMI	CTAG	2 cut(s)	111, 132
SstI	GAGCTC	1 cut(s)	696
StuI	AGGCCT	1 cut(s)	350
StyD4I	CCNGG	3 cut(s)	345, 511, 736
StyI	CCWWGG	1 cut(s)	110
TaaI	ACNGT	2 cut(s)	68, 616
TaiI	ACGT	1 cut(s)	83
TaqI	TCGA	1 cut(s)	708
TasI	AATT	4 cut(s)	146, 450, 579, 641
TatI	WGTACW	1 cut(s)	545
TfiI	GAWTC	1 cut(s)	525
Tru1I	TTAA	3 cut(s)	287, 368, 443
Tru9I	TTAA	3 cut(s)	287, 368, 443
TscAI	CASTG	1 cut(s)	619
TseFI	GTSAC	1 cut(s)	5
TseI	GCWGC	4 cut(s)	35, 269, 296, 602
Tsp45I	GTSAC	1 cut(s)	5
TspRI	CASTG	1 cut(s)	619
VpaK11BI	GGWCC	1 cut(s)	107
XagI	CCTNNNNNAGG	1 cut(s)	114
XapI	RAATTY	1 cut(s)	641
XmaJI	CCTAGG	1 cut(s)	110
XspI	CTAG	2 cut(s)	111, 132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.