MD08G1198300.v1.1

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013242
Physical Location & Seq
Forward (+)
25681728 .. 25685484
3757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1198300.v1.1.491

Sequence Viewer

Length: 2904 bp
ATGTTGCATAGAGCAGCAAGCAACGCATATTCATGGTGGTGGGCCAGCCACATCAGGACAAAGCAGTCCAAATGGCTTGAACAGAGCCTCCAAGATATGGAGGAGAAGGTTCATGATACATTGAAAATCATTGATGAAGATGGGGACTCCTTTGCAAAGAGGGCAGAAATGTATTACAGGAAGAGGCCGGAGCTAGTTGCCTATGTCGAAGAATCTTTTCGCGCCTATCGAGCTTTAGCAGAGCGATATGATCACTTGTCAAGAGACCTTCAGAGTGCCAACCGCACCATTGCCACAGTTTTTCCTGAGCGAGTTCAGTATGCTATGGAGGACGAGGATGATGAGATTGCTTCTCAAACATCAACTTCTTCTGACGGTCCTAACAAAGTGTCACCGGATGCATCCAAAAACATTCCACAAGTCCCCAAGGCCCCCAAATATTTGAGGAGCAAATCCACGTTATTGTCGAGGAAGGGCACGCTTAAGAGAAATGCAAGTTCCGCTAAAGCTGCCCTAGCGGCTACTCTGAGGTCGGGGCTGACCAAAGAGGAGGCACTGGGAGAAATCGATAAGCTTCAAAAGGATATTTTGGCGCTGCAGACGGAAAAGGAGTTTGTGAAGGTTTTTTTCGAACGCGGATATGACAGGTATTGGGAGTTTGAAAATCAGATCACTGATATGCAAAAAAGAGTCTGCAAGTTGCAAGATGAGTATGGCATTGGCACTGTTATCGAAGACAACGATGCTCGAACTTTGATGGCTGCCACGGCTTTGAAGGCATGCAAAGACAGCTTAACAAATCTGCAGGATAAGAAAGAGAGATCAGAAGAAGACGCGAGAGTGGAGCGCCAACGGGTTAGAGAAGCCTGCAGCAAGTTTGAGAGCCTCAGAAATGAATTTCGTTCGAAACACACAGACTGGTGCGACACTGATGGGTATGATTACGAGAATATTGATGTAGAATCAATAAGTCTAGATCAAGAAATACATGATGATCAGGACTCGTTGCGTTCTAGGGGTTTGGAACAGCTCGATACGAATTCAAACACCTCAATTTCTATGACAGAACTAGCAGAGAAAATTGATAGACTTGTGAACAAGATAGTGAATCTGGAAACCGCAGTCACTTCTCAGAATGCTTTAGTAAATAGATTAAAATCAGAAAACGACGAGCTTCAGGCGCACACTCGGAGCTTGGAAGAGGAAAAAGAGACTCTAATGGAAAATGCGGACAAGATGAGCAAACGGCTAAAGGAGTTGGAGGAAGAGCTGCGCCGAATTAGAAATTTAAACAGAAAAGTGGAAGACCAAAATAACAACATCCAAACACATTTTACAGAAGCTAGCTGTAATCTTTACCATCTGTCAGGAAAACTGAATAGTGTGAAGCATGAAGAGGAGGATGAGAATGCCGGATTGTTCCAAGATGTGAAAGCAGTTGATGCCAAACGTGAAAAAGAGATTAAAGAAGATTCAAATAAGGCAGTTCTTGATGATGATTCGGCGGTCTTGGAAGACAAAATGTCAGAAGGTGAAATTAAAAAGAAGAATGTTACAATTCTCGAAACTTCTATCAAGGAGGAAGACGAAGAAGAAAAGCAATCAGACCAACCAATGCAGCATGAACAGGGAGAGGCGCAAGATTTGTCTGAGGATGTTGATCCACAGGATTTGGAACTGGAGAAGGAAGAACAACCAAACTGGAGACAGCTGTTCCTAAAAGGGTTAGAGGATAGAGAAAAGGTTTTGCTGGAGGAGTATACTTCAATTCTCCGCGATTACAAGGACGCAAGGAGGAAGCTCGGCGAAGTGGAAAAGAAAAACCGGGATAACATCTTCGATTTAGCAATGGAGATAAGGGAATTAAGGGGTGCTGTTGCCTCCAAAGACAAAGAGATTAAACATTTGAAGCAAAAACTCGGTTCCCCAGAAACAAATCCGGAGGACAGTCCTGCAACAGTATATAAATTTCCAACTCAGGAAGGCCCCCTTGAGAGTCCAACTCAAGTCGTTGCATCCCCATACTCAACTGTTGCATCACCAAATTTGGATAAAGAGATGATTTCCAGCTACCTTGGTGAACAAGGAATAGAGAAATTTGAGGACTCATCAGGAAACTTAAAGGTCTTACCGAGAAAGGAGGAAGAGAGAATAATGAGGAGAAGACATACTGTTAGACCCCATTCTGTTTCAGCAATAGAAGGAAGGTTCCGTTCAGACATTGATGAGCTGTTGGAGGAAAATTTAGAATTTTGGCTGAGGTTTAGTACCTCGGTTCATCAGATACAAAAATTCCACACTTCGATACAGGATTTACAGTCTGAGTTGTTGAAACTAAAGAACAAAAAGAAGCATGATGGACATGCTTTACAATCGGATGCCAGGCCAATATACCGACACCTAAGAGAGATACAAACGGAACTGTCATTATGGTTGGAACACAATGCAGTACTAAAAGATGACTTGCAGGGTAGATTATCATCCTTGTGCCATATTCAAGATGAGATATCGAGACTCTCCAGTTTAAGTTCCGAAGGAGAAAATATGGAGCTTATAAGCAAATATCAGGCGGCAAAGTTTCAAGGCGAGGTTCTCAACATGAAGCAGGAGAATAATAAGGTTGCAGATGAACTGCAGGCAGGCCTTAACCGTGTTGATGGACTCAAGGTTGAGGTCGAGAAGACACTAGCAAGACTCGATGGGGAACTTGGGATTTCGGTATCAAAGAGCATTAACAACCCCAAACGCTCAACGAGTAAGTCTCGAATTCCCTTGCGGTCTTTCTTGTTTGGGGTTAAGTTGAGAAGGCAAAGGCCATCAATCTTTTCCTGCGCCAGCCCGTCATTACAGAAACAGCACAGTGATTTAGGAGCTGCTGCGCCACCTCCAGAACAACCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0008092 GO:0016020 GO:0044464 GO:0044877 GO:0051015 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

968

Amino Acids

111.19

Weight (kDa)

5.35

Isoelectric Point (pI)

61.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 11 - 84 1.2e-34 KIP1-like protein
NET2A PF25014 234 - 462 5.7e-83 NET2A-like domain
NET2A_C PF24918 734 - 906 9.7e-77 NET2A, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013242)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09720 AT1G58215
fragaria_vesca FvH4_5g34781
malus_domestica MD08G1198300.v1.1 MD15G1385600.v1.1
prunus_persica Prupe.1G532400_v2.0.a1
pyrus_communis pycom08g17090 pycom08g17120
rosa_chinensis RchiOBHm_Chr7g0235421
rosa_laevigata RLG00000001161
rosa_multiflora Rmu_sc0042260.1_g000001
rosa_roxburghii Rroxscaffold_3G00226040
rosa_rugosa Rorug07G0290800
rosa_samantha Rh7AG445300 Rh7BG417500 Rh7CG466000 Rh7DG434000
rosa_wichuraiana Rw7G037000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2555
AasI GACNNNNNNGTC 1 cut(s) 64
AccB7I CCANNNNNTGG 1 cut(s) 97
AccI GTMKAC 1 cut(s) 1760
AccII CGCG 4 cut(s) 222, 636, 836, 1776
AccIII TCCGGA 1 cut(s) 1939
AclWI GGATC 1 cut(s) 1655
AcuI CTGAAG 2 cut(s) 254, 1160
AfaI GTAC 2 cut(s) 2269, 2451
AfiI CCNNNNNNNGG 1 cut(s) 97
AflII CTTAAG 1 cut(s) 482
AhdI GACNNNNNGTC 1 cut(s) 1522
AjnI CCWGG 1 cut(s) 2381
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
Alw26I GTCTC 5 cut(s) 258, 1205, 1699, 2506, 2766
AlwI GGATC 1 cut(s) 1655
Aor13HI TCCGGA 1 cut(s) 1939
AoxI GGCC 7 cut(s) 42, 185, 429, 1984, 2384, 2641, 2813
ApeKI GCWGC 9 cut(s) 14, 509, 595, 761, 870, 1270, 1618, 2873, 2876
Asp700I GAANNNNTTC 1 cut(s) 216
AspLEI GCGC 8 cut(s) 224, 595, 849, 1183, 1275, 1639, 2834, 2881
AspS9I GGNCC 4 cut(s) 42, 377, 430, 1985
AsuC2I CCSGG 1 cut(s) 1826
AsuHPI GGTGA 4 cut(s) 384, 1544, 2031, 2090
AsuII TTCGAA 2 cut(s) 630, 905
AsuNHI GCTAGC 1 cut(s) 1343
AvaII GGWCC 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 479
BbsI GAAGAC 7 cut(s) 741, 837, 1311, 1521, 1590, 2170, 2687
BbvCI CCTCAGC 1 cut(s) 2258
BbvI GCAGC 9 cut(s) 26, 496, 582, 748, 882, 1257, 1630, 2860, 2863
BccI CCATC 8 cut(s) 134, 751, 926, 1368, 2351, 2651, 2693, 2824
BceAI ACGGC 2 cut(s) 783, 1262
BcgI CGANNNNNNTGC 2 cut(s) 712, 746
BciT130I CCWGG 1 cut(s) 2383
BclI TGATCA 2 cut(s) 250, 994
BcnI CCSGG 1 cut(s) 1826
BcoDI GTCTC 5 cut(s) 258, 1205, 1699, 2506, 2766
BfaI CTAG 7 cut(s) 194, 515, 974, 1014, 1070, 1344, 2687
BfmI CTRYAG 4 cut(s) 596, 803, 868, 2633
BfoI RGCGCY 2 cut(s) 596, 850
BfrI CTTAAG 1 cut(s) 482
BglI GCCNNNNNGGC 1 cut(s) 518
BmcAI AGTACT 1 cut(s) 2451
Bme1390I CCNGG 2 cut(s) 1826, 2383
Bme18I GGWCC 1 cut(s) 377
BmeRI GACNNNNNGTC 1 cut(s) 1522
BmgT120I GGNCC 4 cut(s) 42, 377, 430, 1985
BmiI GGNNCC 4 cut(s) 432, 1924, 1987, 2210
BmrFI CCNGG 2 cut(s) 1826, 2383
BmrI ACTGGG 1 cut(s) 566
BmsI GCATC 7 cut(s) 388, 410, 733, 1432, 2024, 2045, 2368
BmtI GCTAGC 1 cut(s) 1347
BmuI ACTGGG 1 cut(s) 566
BpiI GAAGAC 7 cut(s) 741, 837, 1311, 1521, 1590, 2170, 2687
BplI GAGNNNNNCTC 4 cut(s) 1987, 2019, 2746, 2778
BpmI CTGGAG 5 cut(s) 1700, 1723, 1772, 2503, 2871
Bpu10I CCTNAGC 2 cut(s) 306, 2258
Bpu14I TTCGAA 2 cut(s) 630, 905
BpuEI CTTGAG 3 cut(s) 1989, 2012, 2648
BpuMI CCSGG 1 cut(s) 1826
Bsa29I ATCGAT 1 cut(s) 567
BsaBI GATNNNNATC 3 cut(s) 1156, 1659, 2479
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 4 cut(s) 426, 765, 2074, 2271
BsaWI WCCGGW 2 cut(s) 394, 1939
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 5 cut(s) 561, 923, 1683, 1706, 2520
Bse3DI GCAATG 2 cut(s) 288, 1854
Bse8I GATNNNNATC 3 cut(s) 1156, 1659, 2479
BseAI TCCGGA 1 cut(s) 1939
BseBI CCWGG 1 cut(s) 2383
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 4 cut(s) 426, 765, 2074, 2271
BseGI GGATG 9 cut(s) 343, 401, 403, 1320, 1408, 1660, 2015, 2383, 2480
BseJI GATNNNNATC 3 cut(s) 1156, 1659, 2479
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 2 cut(s) 288, 1854
BseMII CTCAG 8 cut(s) 297, 518, 901, 1145, 1641, 1991, 2249, 2313
BseNI ACTGG 5 cut(s) 561, 923, 1683, 1706, 2520
BseRI GAGGAG 6 cut(s) 116, 460, 563, 1412, 1769, 2173
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 9 cut(s) 26, 496, 582, 748, 882, 1257, 1630, 2860, 2863
Bsh1236I CGCG 4 cut(s) 222, 636, 836, 1776
BshFI GGCC 7 cut(s) 44, 187, 431, 1986, 2386, 2643, 2815
BshVI ATCGAT 1 cut(s) 567
BsiSI CCGG 5 cut(s) 188, 395, 1413, 1825, 1940
BslFI GGGAC 2 cut(s) 158, 407
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 5 cut(s) 258, 1205, 1699, 2506, 2766
BsmFI GGGAC 2 cut(s) 158, 407
BsmI GAATGC 2 cut(s) 1141, 1414
BsnI GGCC 7 cut(s) 44, 187, 431, 1986, 2386, 2643, 2815
Bso31I GGTCTC 1 cut(s) 258
Bsp119I TTCGAA 2 cut(s) 630, 905
Bsp1286I GDGCHC 1 cut(s) 479
Bsp13I TCCGGA 1 cut(s) 1939
Bsp143I GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
BspANI GGCC 7 cut(s) 44, 187, 431, 1986, 2386, 2643, 2815
BspCNI CTCAG 8 cut(s) 298, 519, 900, 1144, 1642, 1990, 2250, 2314
BspDI ATCGAT 1 cut(s) 567
BspEI TCCGGA 1 cut(s) 1939
BspFNI CGCG 4 cut(s) 222, 636, 836, 1776
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 4 cut(s) 432, 1924, 1987, 2210
BspMAI CTGCAG 4 cut(s) 600, 807, 872, 2637
BspOI GCTAGC 1 cut(s) 1347
BspPI GGATC 1 cut(s) 1655
BspQI GCTCTTC 1 cut(s) 1260
BspT104I TTCGAA 2 cut(s) 630, 905
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 258
BsrDI GCAATG 2 cut(s) 288, 1854
BsrI ACTGG 5 cut(s) 561, 923, 1683, 1706, 2520
BssECI CCNNGG 4 cut(s) 426, 765, 2074, 2271
BssMI GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
BssNAI GTATAC 1 cut(s) 1761
BssT1I CCWWGG 2 cut(s) 426, 2074
Bst1107I GTATAC 1 cut(s) 1761
Bst2UI CCWGG 1 cut(s) 2383
Bst6I CTCTTC 5 cut(s) 176, 1194, 1260, 1389, 2139
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 1442
BstBI TTCGAA 2 cut(s) 630, 905
BstC8I GCNNGC 9 cut(s) 19, 46, 479, 781, 868, 1345, 2637, 2641, 2836
BstDEI CTNAG 9 cut(s) 306, 527, 887, 1131, 1650, 1977, 2258, 2322, 2402
BstDSI CCRYGG 1 cut(s) 765
BstF5I GGATG 9 cut(s) 343, 401, 403, 1320, 1408, 1660, 2015, 2383, 2480
BstFNI CGCG 4 cut(s) 222, 636, 836, 1776
BstH2I RGCGCY 2 cut(s) 596, 850
BstHHI GCGC 8 cut(s) 224, 595, 849, 1183, 1275, 1639, 2834, 2881
BstKTI GATC 6 cut(s) 253, 672, 824, 979, 997, 1663
BstMAI GTCTC 5 cut(s) 258, 1205, 1699, 2506, 2766
BstMBI GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
BstNI CCWGG 1 cut(s) 2383
BstNSI RCATGY 2 cut(s) 783, 2366
BstSCI CCNGG 2 cut(s) 1824, 2381
BstSFI CTRYAG 4 cut(s) 596, 803, 868, 2633
BstSLI GKGCMC 1 cut(s) 479
BstUI CGCG 4 cut(s) 222, 636, 836, 1776
BstV1I GCAGC 9 cut(s) 26, 496, 582, 748, 882, 1257, 1630, 2860, 2863
BstV2I GAAGAC 7 cut(s) 741, 837, 1311, 1521, 1590, 2170, 2687
BstZ17I GTATAC 1 cut(s) 1761
Bsu15I ATCGAT 1 cut(s) 567
BsuRI GGCC 7 cut(s) 44, 187, 431, 1986, 2386, 2643, 2815
BsuTUI ATCGAT 1 cut(s) 567
BtgI CCRYGG 1 cut(s) 765
BtsCI GGATG 9 cut(s) 343, 401, 403, 1320, 1408, 1660, 2015, 2383, 2480
BtsIMutI CAGTG 5 cut(s) 554, 672, 723, 927, 2866
Cac8I GCNNGC 9 cut(s) 19, 46, 479, 781, 868, 1345, 2637, 2641, 2836
CciI TCATGA 1 cut(s) 112
CfoI GCGC 8 cut(s) 224, 595, 849, 1183, 1275, 1639, 2834, 2881
Cfr13I GGNCC 4 cut(s) 42, 377, 430, 1985
ClaI ATCGAT 1 cut(s) 567
CseI GACGC 2 cut(s) 842, 1796
Csp6I GTAC 2 cut(s) 2268, 2450
CspCI CAANNNNNGTGG 2 cut(s) 1653, 1688
CviAII CATG 9 cut(s) 33, 113, 780, 989, 1391, 1622, 2354, 2363, 2599
CviQI GTAC 2 cut(s) 2268, 2450
DdeI CTNAG 9 cut(s) 306, 527, 887, 1131, 1650, 1977, 2258, 2322, 2402
DpnI GATC 6 cut(s) 252, 671, 823, 978, 996, 1662
DpnII GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
DraI TTTAAA 1 cut(s) 1290
DrdI GACNNNNNNGTC 1 cut(s) 64
DriI GACNNNNNGTC 1 cut(s) 1522
DseDI GACNNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 5 cut(s) 176, 1194, 1260, 1389, 2139
Eam1105I GACNNNNNGTC 1 cut(s) 1522
EarI CTCTTC 5 cut(s) 176, 1194, 1260, 1389, 2139
Eco130I CCWWGG 2 cut(s) 426, 2074
Eco147I AGGCCT 1 cut(s) 2643
Eco31I GGTCTC 1 cut(s) 258
Eco32I GATATC 1 cut(s) 2508
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 2 cut(s) 254, 1160
EcoO109I RGGNCCY 2 cut(s) 430, 1985
EcoRI GAATTC 2 cut(s) 1039, 2766
EcoRII CCWGG 1 cut(s) 2381
EcoRV GATATC 1 cut(s) 2508
EcoT14I CCWWGG 2 cut(s) 426, 2074
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 426, 2074
FaeI CATG 9 cut(s) 36, 116, 783, 992, 1394, 1625, 2357, 2366, 2602
FalI AAGNNNNNCTT 2 cut(s) 1974, 2006
FaqI GGGAC 2 cut(s) 158, 407
FatI CATG 9 cut(s) 32, 112, 779, 988, 1390, 1621, 2353, 2362, 2598
FbaI TGATCA 2 cut(s) 250, 994
FblI GTMKAC 1 cut(s) 1760
FokI GGATG 9 cut(s) 350, 388, 410, 1307, 1415, 1667, 2002, 2390, 2467
FspBI CTAG 7 cut(s) 194, 515, 974, 1014, 1070, 1344, 2687
GlaI GCGC 8 cut(s) 223, 594, 848, 1182, 1274, 1638, 2833, 2880
GsuI CTGGAG 5 cut(s) 1700, 1723, 1772, 2503, 2871
HaeII RGCGCY 2 cut(s) 596, 850
HaeIII GGCC 7 cut(s) 44, 187, 431, 1986, 2386, 2643, 2815
HapII CCGG 5 cut(s) 188, 395, 1413, 1825, 1940
HgaI GACGC 2 cut(s) 842, 1796
HhaI GCGC 8 cut(s) 224, 595, 849, 1183, 1275, 1639, 2834, 2881
Hin1II CATG 9 cut(s) 36, 116, 783, 992, 1394, 1625, 2357, 2366, 2602
Hin6I GCGC 8 cut(s) 222, 593, 847, 1181, 1273, 1637, 2832, 2879
HinP1I GCGC 8 cut(s) 222, 593, 847, 1181, 1273, 1637, 2832, 2879
HindIII AAGCTT 1 cut(s) 572
HpaII CCGG 5 cut(s) 188, 395, 1413, 1825, 1940
HphI GGTGA 4 cut(s) 384, 1544, 2031, 2090
Hpy166II GTNNAC 3 cut(s) 1096, 1761, 2081
Hpy8I GTNNAC 3 cut(s) 1096, 1761, 2081
Hpy99I CGWCG 1 cut(s) 1172
HpyCH4IV ACGT 2 cut(s) 458, 1450
HpyF3I CTNAG 9 cut(s) 306, 527, 887, 1131, 1650, 1977, 2258, 2322, 2402
HpySE526I ACGT 2 cut(s) 458, 1450
Hsp92II CATG 9 cut(s) 36, 116, 783, 992, 1394, 1625, 2357, 2366, 2602
HspAI GCGC 8 cut(s) 222, 593, 847, 1181, 1273, 1637, 2832, 2879
Kpn2I TCCGGA 1 cut(s) 1939
Ksp22I TGATCA 2 cut(s) 250, 994
Kzo9I GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
LguI GCTCTTC 1 cut(s) 1260
LmnI GCTCC 6 cut(s) 190, 447, 844, 1191, 2548, 2870
Lsp1109I GCAGC 9 cut(s) 26, 496, 582, 748, 882, 1257, 1630, 2860, 2863
LweI GCATC 7 cut(s) 388, 410, 733, 1432, 2024, 2045, 2368
MaeI CTAG 7 cut(s) 194, 515, 974, 1014, 1070, 1344, 2687
MaeII ACGT 2 cut(s) 458, 1450
MaeIII GTNAC 3 cut(s) 390, 1123, 1552
MalI GATC 6 cut(s) 252, 671, 823, 978, 996, 1662
MboI GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
MhlI GDGCHC 1 cut(s) 479
MlyI GAGTC 9 cut(s) 140, 699, 995, 1207, 2005, 2099, 2508, 2655, 2688
MmeI TCCRAC 5 cut(s) 1239, 1997, 2024, 2214, 2415
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 1939
MroXI GAANNNNTTC 1 cut(s) 216
MslI CAYNNNNRTG 4 cut(s) 31, 37, 677, 2485
MspA1I CMGCKG 1 cut(s) 1711
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 5 cut(s) 188, 395, 1413, 1825, 1940
MspR9I CCNGG 2 cut(s) 1826, 2383
Mva1269I GAATGC 2 cut(s) 1141, 1414
MvaI CCWGG 1 cut(s) 2383
MvnI CGCG 4 cut(s) 222, 636, 836, 1776
NciI CCSGG 1 cut(s) 1826
NdeII GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
NheI GCTAGC 1 cut(s) 1343
NlaIII CATG 9 cut(s) 36, 116, 783, 992, 1394, 1625, 2357, 2366, 2602
NlaIV GGNNCC 4 cut(s) 432, 1924, 1987, 2210
NmeAIII GCCGAG 1 cut(s) 1782
NmuCI GTSAC 2 cut(s) 390, 1123
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 2 cut(s) 783, 2366
NspV TTCGAA 2 cut(s) 630, 905
PaeI GCATGC 1 cut(s) 783
PagI TCATGA 1 cut(s) 112
PceI AGGCCT 1 cut(s) 2643
PciSI GCTCTTC 1 cut(s) 1260
PcsI WCGNNNNNNNCGW 3 cut(s) 226, 464, 738
PctI GAATGC 2 cut(s) 1141, 1414
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 5 cut(s) 212, 962, 1108, 1472, 1499
PflMI CCANNNNNTGG 1 cut(s) 97
PleI GAGTC 9 cut(s) 140, 698, 995, 1207, 2004, 2099, 2508, 2655, 2688
PpsI GAGTC 9 cut(s) 140, 698, 995, 1207, 2004, 2099, 2508, 2655, 2688
PsiI TTATAA 1 cut(s) 2555
Psp6I CCWGG 1 cut(s) 2381
PspGI CCWGG 1 cut(s) 2381
PspN4I GGNNCC 4 cut(s) 432, 1924, 1987, 2210
PspPI GGNCC 4 cut(s) 42, 377, 430, 1985
PstI CTGCAG 4 cut(s) 600, 807, 872, 2637
PvuII CAGCTG 1 cut(s) 1711
RsaI GTAC 2 cut(s) 2269, 2451
RsaNI GTAC 2 cut(s) 2268, 2450
RseI CAYNNNNRTG 4 cut(s) 31, 37, 677, 2485
SapI GCTCTTC 1 cut(s) 1260
Sau3AI GATC 6 cut(s) 250, 669, 821, 976, 994, 1660
Sau96I GGNCC 4 cut(s) 42, 377, 430, 1985
ScaI AGTACT 1 cut(s) 2451
SchI GAGTC 9 cut(s) 140, 699, 995, 1207, 2005, 2099, 2508, 2655, 2688
ScrFI CCNGG 2 cut(s) 1826, 2383
SduI GDGCHC 1 cut(s) 479
SfaNI GCATC 7 cut(s) 388, 410, 733, 1432, 2024, 2045, 2368
SfcI CTRYAG 4 cut(s) 596, 803, 868, 2633
SfuI TTCGAA 2 cut(s) 630, 905
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 4 cut(s) 31, 37, 677, 2485
SmlI CTYRAG 4 cut(s) 482, 1991, 2004, 2663
SmoI CTYRAG 4 cut(s) 482, 1991, 2004, 2663
SphI GCATGC 1 cut(s) 783
SseBI AGGCCT 1 cut(s) 2643
SspI AATATT 2 cut(s) 440, 952
SspMI CTAG 7 cut(s) 194, 515, 974, 1014, 1070, 1344, 2687
StuI AGGCCT 1 cut(s) 2643
StyD4I CCNGG 2 cut(s) 1824, 2381
StyI CCWWGG 2 cut(s) 426, 2074
TaiI ACGT 2 cut(s) 461, 1453
TatI WGTACW 1 cut(s) 2449
TauI GCSGC 2 cut(s) 521, 2573
TfiI GAWTC 5 cut(s) 212, 962, 1108, 1472, 1499
TscAI CASTG 5 cut(s) 561, 679, 730, 934, 2866
TseFI GTSAC 2 cut(s) 390, 1123
TseI GCWGC 9 cut(s) 14, 509, 595, 761, 870, 1270, 1618, 2873, 2876
Tsp45I GTSAC 2 cut(s) 390, 1123
TspDTI ATGAA 9 cut(s) 21, 101, 150, 909, 1407, 1638, 2267, 2615, 2643
TspGWI ACGGA 3 cut(s) 617, 2201, 2432
TspRI CASTG 5 cut(s) 561, 679, 730, 934, 2866
Van91I CCANNNNNTGG 1 cut(s) 97
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 377
XbaI TCTAGA 1 cut(s) 973
XceI RCATGY 2 cut(s) 783, 2366
XmiI GTMKAC 1 cut(s) 1760
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 7 cut(s) 194, 515, 974, 1014, 1070, 1344, 2687
ZrmI AGTACT 1 cut(s) 2451
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.