Rorug07G0290800

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001174
Physical Location & Seq
Reverse (-)
28356637 .. 28376575
19939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0290800.1

Sequence Viewer

Length: 960 bp
ATGGCAGTTCAGAATGGAAGGTTCTGTGTTACAGGGGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTTTCCAAGAACTGCATAGTCCACGGAACCGTCAGAGAACCCACGGATGACAAGAACTCTCACTTGAGAAAGCTTGACAAAGCATCTGAGAACCTGAAACTATTCAAGGCGGACTTATTGGATTATGACTCCCTTTGCTTGGCAATTGATGGATGTGATGGAGTATTTCATGTTGCCAGTCCTGTTCCCTCCGGGAGTGTGCCAAATCCTGAGGTACAGTTAATTGAACCTGCTGTTAAGGGGACACTTAATGTACTTAAAGCATCTCTTGAAGCAAAAGTCAAGCGAGTTGTTTATGTGTCCTCTGTTGCTGCTGTGGCCATGAATCCTACGTGGGCTGAAGGTCAACTATTGGATGAGTCTTGCTGGTCAGATAAGGAATACTGCAGAAATACTGAGAACTGGTATTGCCTTTCAAAAACTGAAGCAGAATCTGAAGCTCTGCAGTTTGCAAAAACAACCGGGATTGATCTTGTGACTGTTTGTCCCTATCTTATACTCGGCCCAATTCTGCAGTCGACTGTAAATGCAAGTACCATGATCCTCATCAAGCTTTTGAAGGAAGGAAAGGAGTCACTGGAAAACAGGCCCCGGCAGATAGTTGACGTACGGGATTTAGCTGAAGCACTTCTTATGGTATATGAGAAGCAGGAGGCTGAAGGAAGATACATATGCAAGTCCCACAATGTTAAGAATGGGGATCTAGTTGAGAAGCTGAGGCGCCTTTATCCTAATTACAATTATCCGAAGAACTTTGTTGAAGTAGACGAAGACATAAAGGTGAGCTCAGCGAAACTGCAAAGGCTAGGTTGGAGTTACCGGGCATTGGATGAGACTCTCATCGACTCTGTTGAAAGCTACCGTGAAGCTGGACTATTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

319

Amino Acids

35.52

Weight (kDa)

5.69

Isoelectric Point (pI)

29.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 131 1.1e-08 NmrA-like family
Epimerase PF01370 9 - 244 1.3e-27 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 132 1.4e-08 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 202 1.7e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 263 8.2e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 200 1.4e-08 Male sterility protein
NAD_binding_10 PF13460 12 - 132 5.5e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 795
AccI GTMKAC 2 cut(s) 593, 840
AciI CCGC 1 cut(s) 185
AclWI GGATC 2 cut(s) 610, 783
AcoI YGGCCR 1 cut(s) 393
AcuI CTGAAG 5 cut(s) 435, 519, 531, 717, 753
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 4 cut(s) 291, 330, 610, 684
AfiI CCNNNNNNNGG 2 cut(s) 214, 901
AgsI TTSAA 7 cut(s) 181, 302, 347, 492, 634, 836, 929
AhdI GACNNNNNGTC 1 cut(s) 558
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 9 cut(s) 70, 148, 515, 628, 695, 790, 861, 933, 944
AluI AGCT 9 cut(s) 70, 148, 515, 628, 695, 790, 861, 933, 944
Alw21I GWGCWC 1 cut(s) 863
Alw26I GTCTC 1 cut(s) 902
AlwI GGATC 2 cut(s) 610, 783
AlwNI CAGNNNCTG 2 cut(s) 38, 509
AoxI GGCC 3 cut(s) 393, 577, 662
ApeKI GCWGC 1 cut(s) 386
Asp700I GAANNNNTTC 2 cut(s) 176, 702
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 2 cut(s) 578, 663
AsuC2I CCSGG 4 cut(s) 268, 538, 667, 896
AsuHPI GGTGA 1 cut(s) 868
AxyI CCTNAGG 1 cut(s) 285
BalI TGGCCA 1 cut(s) 395
BanI GGYRCC 1 cut(s) 795
BanII GRGCYC 1 cut(s) 863
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 863
BbvCI CCTCAGC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 218, 227
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 4 cut(s) 268, 538, 667, 896
BcoDI GTCTC 1 cut(s) 902
BfaI CTAG 2 cut(s) 779, 881
BfmI CTRYAG 3 cut(s) 460, 518, 587
BfoI RGCGCY 1 cut(s) 799
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 1 cut(s) 387
BlpI GCTNAGC 1 cut(s) 862
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 5 cut(s) 58, 268, 538, 667, 896
BmeRI GACNNNNNGTC 1 cut(s) 558
BmgT120I GGNCC 2 cut(s) 578, 663
BmiI GGNNCC 4 cut(s) 55, 103, 665, 797
BmrFI CCNGG 5 cut(s) 58, 268, 538, 667, 896
BmsI GCATC 2 cut(s) 167, 347
BpiI GAAGAC 1 cut(s) 852
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 791
Bpu1102I GCTNAGC 1 cut(s) 862
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 4 cut(s) 268, 538, 667, 896
BsaAI YACGTR 1 cut(s) 408
BsaBI GATNNNNATC 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 4 cut(s) 57, 97, 117, 665
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 901
Bse1I ACTGG 3 cut(s) 252, 482, 657
Bse21I CCTNAGG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 620
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 4 cut(s) 57, 97, 117, 665
BseGI GGATG 4 cut(s) 127, 233, 436, 910
BseJI GATNNNNATC 1 cut(s) 620
BseLI CCNNNNNNNGG 2 cut(s) 214, 901
BseMII CTCAG 5 cut(s) 153, 276, 462, 782, 876
BseNI ACTGG 3 cut(s) 252, 482, 657
BseXI GCAGC 1 cut(s) 373
BshFI GGCC 3 cut(s) 395, 579, 664
BshNI GGYRCC 1 cut(s) 795
BsiHKAI GWGCWC 1 cut(s) 863
BsiSI CCGG 4 cut(s) 267, 537, 667, 895
BsiWI CGTACG 1 cut(s) 682
BslFI GGGAC 3 cut(s) 331, 546, 739
BslI CCNNNNNNNGG 2 cut(s) 214, 901
BsmAI GTCTC 1 cut(s) 902
BsmFI GGGAC 3 cut(s) 331, 546, 739
BsnI GGCC 3 cut(s) 395, 579, 664
Bsp1286I GDGCHC 1 cut(s) 863
Bsp143I GATC 3 cut(s) 544, 615, 775
Bsp1720I GCTNAGC 1 cut(s) 862
BspACI CCGC 1 cut(s) 185
BspANI GGCC 3 cut(s) 395, 579, 664
BspCNI CTCAG 5 cut(s) 154, 277, 463, 783, 875
BspLI GGNNCC 4 cut(s) 55, 103, 665, 797
BspMAI CTGCAG 3 cut(s) 464, 522, 591
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 2 cut(s) 610, 783
BspT107I GGYRCC 1 cut(s) 795
BsrI ACTGG 3 cut(s) 252, 482, 657
BssECI CCNNGG 4 cut(s) 57, 97, 117, 665
BssMI GATC 3 cut(s) 544, 615, 775
BssNI GRCGYC 1 cut(s) 796
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 5 cut(s) 106, 294, 556, 598, 938
BstACI GRCGYC 1 cut(s) 796
BstBAI YACGTR 1 cut(s) 408
BstDEI CTNAG 5 cut(s) 162, 285, 471, 791, 862
BstDSI CCRYGG 2 cut(s) 97, 117
BstF5I GGATG 4 cut(s) 127, 233, 436, 910
BstH2I RGCGCY 1 cut(s) 799
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 3 cut(s) 547, 618, 778
BstMAI GTCTC 1 cut(s) 902
BstMBI GATC 3 cut(s) 544, 615, 775
BstMWI GCNNNNNNNGC 2 cut(s) 51, 392
BstNI CCWGG 1 cut(s) 58
BstSCI CCNGG 5 cut(s) 56, 266, 536, 665, 894
BstSFI CTRYAG 3 cut(s) 460, 518, 587
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 3 cut(s) 395, 579, 664
BtgI CCRYGG 2 cut(s) 97, 117
BtsCI GGATG 4 cut(s) 127, 233, 436, 910
BtsIMutI CAGTG 1 cut(s) 650
BveI ACCTGC 1 cut(s) 313
CaiI CAGNNNCTG 2 cut(s) 38, 509
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 578, 663
Csp6I GTAC 4 cut(s) 290, 329, 609, 683
CviAII CATG 3 cut(s) 245, 397, 613
CviQI GTAC 4 cut(s) 290, 329, 609, 683
DdeI CTNAG 5 cut(s) 162, 285, 471, 791, 862
DinI GGCGCC 1 cut(s) 797
DpnI GATC 3 cut(s) 546, 617, 777
DpnII GATC 3 cut(s) 544, 615, 775
DriI GACNNNNNGTC 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 393
Eam1105I GACNNNNNGTC 1 cut(s) 558
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 863
Eco53kI GAGCTC 1 cut(s) 861
Eco57I CTGAAG 5 cut(s) 435, 519, 531, 717, 753
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 861
EcoO109I RGGNCCY 1 cut(s) 663
EcoRII CCWGG 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 863
EgeI GGCGCC 1 cut(s) 797
EheI GGCGCC 1 cut(s) 797
FaeI CATG 3 cut(s) 248, 400, 616
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 327, 359, 690, 722
FaqI GGGAC 3 cut(s) 331, 546, 739
FatI CATG 3 cut(s) 244, 396, 612
FauNDI CATATG 1 cut(s) 746
FblI GTMKAC 2 cut(s) 593, 840
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 4 cut(s) 134, 240, 443, 917
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 2 cut(s) 779, 881
GlaI GCGC 1 cut(s) 797
GluI GCNGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 799
HaeIII GGCC 3 cut(s) 395, 579, 664
HapII CCGG 4 cut(s) 267, 537, 667, 895
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 3 cut(s) 248, 400, 616
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 3 cut(s) 422, 594, 679
HindII GTYRAC 3 cut(s) 422, 594, 679
HindIII AAGCTT 3 cut(s) 68, 146, 626
HinfI GANTC 7 cut(s) 203, 400, 434, 506, 647, 910, 920
HpaII CCGG 4 cut(s) 267, 537, 667, 895
HphI GGTGA 1 cut(s) 868
Hpy166II GTNNAC 5 cut(s) 97, 422, 594, 679, 841
Hpy188I TCNGA 6 cut(s) 12, 110, 163, 448, 511, 822
Hpy188III TCNNGA 2 cut(s) 284, 344
Hpy8I GTNNAC 5 cut(s) 97, 422, 594, 679, 841
HpyAV CCTTC 5 cut(s) 12, 410, 628, 632, 728
HpyCH4III ACNGT 5 cut(s) 106, 294, 556, 598, 938
HpyCH4IV ACGT 2 cut(s) 407, 681
HpyCH4V TGCA 9 cut(s) 90, 462, 520, 527, 589, 605, 750, 874, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 392
HpyF3I CTNAG 5 cut(s) 162, 285, 471, 791, 862
HpySE526I ACGT 2 cut(s) 407, 681
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 3 cut(s) 248, 400, 616
HspAI GCGC 1 cut(s) 796
KasI GGCGCC 1 cut(s) 795
Kzo9I GATC 3 cut(s) 544, 615, 775
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 167, 347
MaeI CTAG 2 cut(s) 779, 881
MaeII ACGT 2 cut(s) 407, 681
MaeIII GTNAC 4 cut(s) 28, 550, 648, 890
MalI GATC 3 cut(s) 546, 617, 777
MboI GATC 3 cut(s) 544, 615, 775
MboII GAAGA 4 cut(s) 65, 750, 835, 857
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 863
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 5 cut(s) 219, 297, 582, 808, 814
MluNI TGGCCA 1 cut(s) 395
Mly113I GGCGCC 1 cut(s) 796
MlyI GAGTC 5 cut(s) 197, 443, 656, 904, 914
MmeI TCCRAC 1 cut(s) 866
MnlI CCTC 7 cut(s) 35, 274, 280, 388, 629, 721, 786
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 2 cut(s) 176, 702
MscI TGGCCA 1 cut(s) 395
MseI TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
MslI CAYNNNNRTG 1 cut(s) 854
Msp20I TGGCCA 1 cut(s) 395
MspI CCGG 4 cut(s) 267, 537, 667, 895
MspR9I CCNGG 5 cut(s) 58, 268, 538, 667, 896
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 2 cut(s) 51, 392
NarI GGCGCC 1 cut(s) 796
NciI CCSGG 4 cut(s) 268, 538, 667, 896
NdeI CATATG 1 cut(s) 746
NdeII GATC 3 cut(s) 544, 615, 775
NlaIII CATG 3 cut(s) 248, 400, 616
NlaIV GGNNCC 4 cut(s) 55, 103, 665, 797
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 2 cut(s) 550, 648
PdmI GAANNNNTTC 2 cut(s) 176, 702
PfeI GAWTC 2 cut(s) 400, 506
Pfl23II CGTACG 1 cut(s) 682
PfoI TCCNGGA 1 cut(s) 266
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 5 cut(s) 197, 442, 655, 904, 914
PluTI GGCGCC 1 cut(s) 799
PpsI GAGTC 5 cut(s) 197, 442, 655, 904, 914
Ppu21I YACGTR 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspLI CGTACG 1 cut(s) 682
PspN4I GGNNCC 4 cut(s) 55, 103, 665, 797
PspPI GGNCC 2 cut(s) 578, 663
PstI CTGCAG 3 cut(s) 464, 522, 591
PstNI CAGNNNCTG 2 cut(s) 38, 509
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 4 cut(s) 291, 330, 610, 684
RsaNI GTAC 4 cut(s) 290, 329, 609, 683
RseI CAYNNNNRTG 1 cut(s) 854
SacI GAGCTC 1 cut(s) 863
SalI GTCGAC 1 cut(s) 592
SaqAI TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 3 cut(s) 544, 615, 775
Sau96I GGNCC 2 cut(s) 578, 663
SchI GAGTC 5 cut(s) 197, 443, 656, 904, 914
ScrFI CCNGG 5 cut(s) 58, 268, 538, 667, 896
SduI GDGCHC 1 cut(s) 863
SfaNI GCATC 2 cut(s) 167, 347
SfcI CTRYAG 3 cut(s) 460, 518, 587
SfoI GGCGCC 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 854
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 5 cut(s) 219, 297, 582, 808, 814
SsiI CCGC 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 795
SspMI CTAG 2 cut(s) 779, 881
SstI GAGCTC 1 cut(s) 863
StyD4I CCNGG 5 cut(s) 56, 266, 536, 665, 894
TaaI ACNGT 5 cut(s) 106, 294, 556, 598, 938
TaiI ACGT 2 cut(s) 410, 684
TaqI TCGA 2 cut(s) 593, 918
TasI AATT 5 cut(s) 219, 297, 582, 808, 814
TatI WGTACW 1 cut(s) 328
TfiI GAWTC 2 cut(s) 400, 506
Tru1I TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
Tru9I TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
TscAI CASTG 1 cut(s) 657
TseFI GTSAC 2 cut(s) 550, 648
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 2 cut(s) 550, 648
TspDTI ATGAA 2 cut(s) 233, 413
TspGWI ACGGA 2 cut(s) 114, 134
TspRI CASTG 1 cut(s) 657
XmiI GTMKAC 2 cut(s) 593, 840
XmnI GAANNNNTTC 2 cut(s) 176, 702
XspI CTAG 2 cut(s) 779, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.