Rh7DG423200

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001174
Physical Location & Seq
Reverse (-)
59831170 .. 59831798
629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG423200.1

Sequence Viewer

Length: 342 bp
ATGTTTTGCATTCCTTTTAGTAATGCCTTTTCTGACATGTTGCTGCAAGAATATAAGGAGAACCCAGATTATGCACGTTGTGTTGCTATTAATTGTAGCATCTGCATCTTTGAGGGAGATGATAAAACCTGGTGCCTTGGCTATTATTTCACCTATAGCAGTTGGTATTGCGTTCTGAATATTTGGATACTATCCGGAGGCAAAGGAAGTGATTGTCATAAGGGTGCAATTACAGGAGACATAGTGGGAGACCCATTCAAAGACACAGCTGGACCTTCACTTCATGTCCTTATTAAAATGCTTGCTACAAAAATGGTGCTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

113

Amino Acids

12.63

Weight (kDa)

5.09

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 66 - 107 6.6e-16 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 194
AdeI CACNNNGTG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 1 cut(s) 259
AjnI CCWGG 1 cut(s) 128
AluBI AGCT 1 cut(s) 269
AluI AGCT 1 cut(s) 269
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 231, 243
Aor13HI TCCGGA 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 43
AseI ATTAAT 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 130
BciVI GTATCC 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 231, 243
BfmI CTRYAG 1 cut(s) 154
BfuI GTATCC 1 cut(s) 180
BisI GCNGC 1 cut(s) 44
BlsI GCNGC 1 cut(s) 45
Bme1390I CCNGG 1 cut(s) 130
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 2 cut(s) 134, 327
BmrFI CCNGG 1 cut(s) 130
BmsI GCATC 2 cut(s) 108, 114
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
BseAI TCCGGA 1 cut(s) 194
BseBI CCWGG 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 1 cut(s) 195
BsmAI GTCTC 2 cut(s) 231, 243
BsmI GAATGC 1 cut(s) 9
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 321
Bsp13I TCCGGA 1 cut(s) 194
BspEI TCCGGA 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 134, 327
BspT107I GGYRCC 1 cut(s) 132
BspTNI GGTCTC 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 136
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 303
BstMAI GTCTC 2 cut(s) 231, 243
BstNI CCWGG 1 cut(s) 130
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 128
BstSFI CTRYAG 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 30
BsuI GTATCC 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 272
CsiI ACCWGGT 1 cut(s) 128
CviAII CATG 3 cut(s) 37, 284, 322
CviJI RGCY 3 cut(s) 141, 269, 326
CviKI_1 RGCY 3 cut(s) 141, 269, 326
DraIII CACNNNGTG 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 3 cut(s) 40, 287, 325
FaiI YATR 8 cut(s) 38, 54, 72, 156, 219, 242, 285, 323
FatI CATG 3 cut(s) 36, 283, 321
Fnu4HI GCNGC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 44
GluI GCNGC 1 cut(s) 44
HapII CCGG 1 cut(s) 195
Hin1II CATG 3 cut(s) 40, 287, 325
HpaII CCGG 1 cut(s) 195
HphI GGTGA 1 cut(s) 142
Hpy188I TCNGA 3 cut(s) 34, 177, 341
Hpy188III TCNNGA 1 cut(s) 195
HpyAV CCTTC 1 cut(s) 285
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 5 cut(s) 9, 46, 74, 105, 227
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 3 cut(s) 40, 287, 325
Kpn2I TCCGGA 1 cut(s) 194
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 6 cut(s) 78, 115, 142, 208, 219, 255
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 2 cut(s) 108, 114
MabI ACCWGGT 1 cut(s) 128
MaeII ACGT 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 2 cut(s) 91, 228
MnlI CCTC 2 cut(s) 106, 191
MroI TCCGGA 1 cut(s) 194
MseI TTAA 2 cut(s) 90, 294
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 130
Mva1269I GAATGC 1 cut(s) 9
MvaI CCWGG 1 cut(s) 130
NlaIII CATG 3 cut(s) 40, 287, 325
NlaIV GGNNCC 2 cut(s) 134, 327
NspI RCATGY 1 cut(s) 40
PciI ACATGT 1 cut(s) 36
PctI GAATGC 1 cut(s) 9
PkrI GCNGC 1 cut(s) 45
PscI ACATGT 1 cut(s) 36
PshBI ATTAAT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 128
PspGI CCWGG 1 cut(s) 128
PspN4I GGNNCC 2 cut(s) 134, 327
PspPI GGNCC 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 2 cut(s) 90, 294
SatI GCNGC 1 cut(s) 44
Sau96I GGNCC 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 130
SduI GDGCHC 1 cut(s) 321
SetI ASST 5 cut(s) 79, 131, 155, 271, 277
SexAI ACCWGGT 1 cut(s) 128
SfaNI GCATC 2 cut(s) 108, 114
SfcI CTRYAG 1 cut(s) 154
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 2 cut(s) 91, 228
SspI AATATT 1 cut(s) 181
StyD4I CCNGG 1 cut(s) 128
StyI CCWWGG 1 cut(s) 136
TaiI ACGT 1 cut(s) 79
TasI AATT 2 cut(s) 91, 228
Tru1I TTAA 2 cut(s) 90, 294
Tru9I TTAA 2 cut(s) 90, 294
TseI GCWGC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 272
VspI ATTAAT 1 cut(s) 90
XceI RCATGY 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.