Rorug03G0200700

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001174
Physical Location & Seq
Forward (+)
17692495 .. 17695364
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0200700.1

Sequence Viewer

Length: 1473 bp
ATGGCTAATGCAAGTTGTGAACCTGAAGGAGGAGATTGTCATGAGGATGAGATTATGGTAGGAAGATTCACTAGTCTTCCAGATCAAGTTGTGCATCACATTGTTTCTTTCCTAGCTATCACTGACCTCACTCGTTTTGGCTGTGTGTCCAAGAAGTGTAGAGAAATTTATCTAACAAGTCCATCCTTGAAGTTTGATGAATTTTCCTCGGCTAATACATCCTCATGTCATAAACGGATGAATTTGTTGAGGTCTTTGGATAAGTTCTTGATTCATCGTGGGGAGAATAAGATACACCAATTTTGTATTCGTTGGGTTCCCCCTGAGAACTTTGACCATGAAACACCATGCTTTTGTGGTTGTAATGAGAAGTCTGGAACGATGGCGTGGATGCAGTATGCAGTAAGGTGTAAAGTGGAAGTGCTTGATCTTGAGATCTCTATATTTGAGAAGGCGATGCCATTAGCATTTCCATTTTTCATCCTTCAGTGTAAATCTTTGAGGTCTCTATCGCTGGACATGAATTGTATGATTCATACACCCTCTTTCGCCGTTTTCTCTAGTCTCAAGTGCTTGGAGCTGAAAAATGTTAGAATATTGGATGAGTCATTTTTCAAATGGATCTCAAATTCCTGCAAATGCATTGAGGAATTAGATCTTGATGATGTCCATGGAATTAGAAATATCAACATTGAGAGCTCTTCTTTGAAATCATTTAAATTTGGGTCTTGCAATCAGACTCGCTTCTGTCATCTTAAAATCTCTGGTGAGAAACTCATAGCCATAGTTGTCTACTGGAGTTTTTATTCATCTCTCAACAAATCGTTTAGTCTTTCTGCTCCGAATCTGAAAAGATTCTATTGGTTTGGGAATTTGCTGGATCACTCAAATCTCGGAGAACCGGTATGTTTAGAAGATGTTGGATTTTATACAGAGCCTGAAGACGATGAGACGGACTATATAGAGCATGTTTACACTACACACAAGCGCTTTGTTGTTCTAAATCCAGAGGCCATGAAGGGACTGTATAAGAGAGGAATCATACCACCTCTGTTTCGTAATACTCGTTATTTGTGTATGCATATTGGCAGCTTTGTTAATGACCTAGTCCCATGGATGGTCTTTCTTTTCCGAAGAATGCCTAATTTGTGTACCTTGTACATCAAGTCTACTCCACCTCTATCTGACTGCAATTCTCATCGTACATGTGGGTTCTATATGGAACACTGGAAGCAGCAGAACTTTGGTTTTATACTTGAGCTTAATGAGGTTACCATTGAGCTTTCCAGAGGACTAAATGGAATTGAGTTAGCAAGGTATATGCTCGAGCATGCTCAGAATTTGAAGAAAATGGTCATAGTTTGTTTACCTCTGCAGTCTGGTTTTGTTAAAAGGCTGTTAGATAAAAGTAAGATGATTTCGAATGGCACGGTTGTGTTCAAGGAAAATCGACAAGAAAGAAAGCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

490

Amino Acids

56.7

Weight (kDa)

7.74

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 127 - 248 3.8e-09 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 129 - 245 3.6e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 792, 1169
AclWI GGATC 2 cut(s) 629, 888
AcsI RAATTY 7 cut(s) 165, 200, 241, 628, 719, 871, 1339
AcuI CTGAAG 3 cut(s) 45, 470, 960
AfaI GTAC 3 cut(s) 1153, 1160, 1204
AfeI AGCGCT 1 cut(s) 989
AfiI CCNNNNNNNGG 2 cut(s) 29, 1117
AflIII ACRYGT 1 cut(s) 1205
AgeI ACCGGT 1 cut(s) 901
AgsI TTSAA 5 cut(s) 190, 616, 709, 1345, 1441
AhlI ACTAGT 1 cut(s) 71
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AleI CACNNNNGTG 1 cut(s) 1433
AluBI AGCT 7 cut(s) 116, 580, 699, 1092, 1261, 1282, 1465
AluI AGCT 7 cut(s) 116, 580, 699, 1092, 1261, 1282, 1465
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 3 cut(s) 510, 569, 944
AlwI GGATC 2 cut(s) 629, 888
AlwNI CAGNNNCTG 1 cut(s) 938
Ama87I CYCGRG 1 cut(s) 1325
Aor51HI AGCGCT 1 cut(s) 989
AoxI GGCC 1 cut(s) 1011
ApeKI GCWGC 2 cut(s) 1089, 1234
ApoI RAATTY 7 cut(s) 165, 200, 241, 628, 719, 871, 1339
AsiGI ACCGGT 1 cut(s) 901
Asp700I GAANNNNTTC 1 cut(s) 854
AspLEI GCGC 1 cut(s) 990
AsuHPI GGTGA 1 cut(s) 779
AsuII TTCGAA 1 cut(s) 1421
AvaI CYCGRG 1 cut(s) 1325
BanII GRGCYC 1 cut(s) 701
BbsI GAAGAC 2 cut(s) 68, 948
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 2 cut(s) 1101, 1246
BccI CCATC 3 cut(s) 190, 376, 1111
BceAI ACGGC 1 cut(s) 536
BcoDI GTCTC 3 cut(s) 510, 569, 944
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 4 cut(s) 72, 113, 561, 1106
BfmI CTRYAG 1 cut(s) 1373
BfoI RGCGCY 1 cut(s) 991
BglII AGATCT 2 cut(s) 435, 655
BisI GCNGC 2 cut(s) 1090, 1235
BlsI GCNGC 2 cut(s) 1091, 1236
BmeT110I CYCGRG 1 cut(s) 1325
BmiI GGNNCC 1 cut(s) 318
BmsI GCATC 3 cut(s) 103, 381, 447
BpiI GAAGAC 2 cut(s) 68, 948
BpmI CTGGAG 1 cut(s) 817
Bpu14I TTCGAA 1 cut(s) 1421
BpuEI CTTGAG 3 cut(s) 452, 551, 1277
BsaI GGTCTC 1 cut(s) 510
BsaJI CCNNGG 3 cut(s) 207, 670, 1112
BsaWI WCCGGW 1 cut(s) 901
BsaXI ACNNNNNCTCC 2 cut(s) 888, 918
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 1117
Bse118I RCCGGY 1 cut(s) 901
Bse1I ACTGG 2 cut(s) 800, 1232
BseDI CCNNGG 3 cut(s) 207, 670, 1112
BseGI GGATG 8 cut(s) 52, 182, 218, 243, 396, 480, 607, 1122
BseLI CCNNNNNNNGG 2 cut(s) 29, 1117
BseMII CTCAG 2 cut(s) 315, 1349
BseNI ACTGG 2 cut(s) 800, 1232
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 2 cut(s) 1101, 1246
BshFI GGCC 1 cut(s) 1013
BshTI ACCGGT 1 cut(s) 901
BsiHKAI GWGCWC 1 cut(s) 701
BsiHKCI CYCGRG 1 cut(s) 1325
BsiSI CCGG 1 cut(s) 902
BslFI GGGAC 2 cut(s) 1035, 1094
BslI CCNNNNNNNGG 2 cut(s) 29, 1117
BsmAI GTCTC 3 cut(s) 510, 569, 944
BsmBI CGTCTC 1 cut(s) 944
BsmFI GGGAC 2 cut(s) 1035, 1094
BsmI GAATGC 1 cut(s) 1143
BsnI GGCC 1 cut(s) 1013
Bso31I GGTCTC 1 cut(s) 510
BsoBI CYCGRG 1 cut(s) 1325
Bsp119I TTCGAA 1 cut(s) 1421
Bsp1286I GDGCHC 1 cut(s) 701
Bsp1407I TGTACA 1 cut(s) 1158
Bsp143I GATC 6 cut(s) 82, 427, 435, 621, 655, 880
Bsp19I CCATGG 2 cut(s) 670, 1112
BspANI GGCC 1 cut(s) 1013
BspCNI CTCAG 2 cut(s) 316, 1348
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 318
BspMAI CTGCAG 1 cut(s) 1377
BspPI GGATC 2 cut(s) 629, 888
BspQI GCTCTTC 1 cut(s) 706
BspT104I TTCGAA 1 cut(s) 1421
BspTNI GGTCTC 1 cut(s) 510
BsrFI RCCGGY 1 cut(s) 901
BsrGI TGTACA 1 cut(s) 1158
BsrI ACTGG 2 cut(s) 800, 1232
BssAI RCCGGY 1 cut(s) 901
BssECI CCNNGG 3 cut(s) 207, 670, 1112
BssMI GATC 6 cut(s) 82, 427, 435, 621, 655, 880
BssT1I CCWWGG 2 cut(s) 670, 1112
Bst4CI ACNGT 2 cut(s) 1026, 1432
Bst6I CTCTTC 1 cut(s) 706
BstAUI TGTACA 1 cut(s) 1158
BstBI TTCGAA 1 cut(s) 1421
BstC8I GCNNGC 1 cut(s) 1332
BstDEI CTNAG 2 cut(s) 324, 1335
BstDSI CCRYGG 2 cut(s) 670, 1112
BstEII GGTNACC 1 cut(s) 1270
BstENI CCTNNNNNAGG 1 cut(s) 27
BstF5I GGATG 8 cut(s) 52, 182, 218, 243, 396, 480, 607, 1122
BstH2I RGCGCY 1 cut(s) 991
BstHHI GCGC 1 cut(s) 990
BstKTI GATC 6 cut(s) 85, 430, 438, 624, 658, 883
BstMAI GTCTC 3 cut(s) 510, 569, 944
BstMBI GATC 6 cut(s) 82, 427, 435, 621, 655, 880
BstNSI RCATGY 3 cut(s) 971, 1209, 1334
BstPI GGTNACC 1 cut(s) 1270
BstSFI CTRYAG 1 cut(s) 1373
BstV1I GCAGC 2 cut(s) 1101, 1246
BstV2I GAAGAC 2 cut(s) 68, 948
BstX2I RGATCY 3 cut(s) 435, 621, 655
BstYI RGATCY 3 cut(s) 435, 621, 655
BsuRI GGCC 1 cut(s) 1013
BtgI CCRYGG 2 cut(s) 670, 1112
BtgZI GCGATG 1 cut(s) 470
BtsCI GGATG 8 cut(s) 52, 182, 218, 243, 396, 480, 607, 1122
BtsIMutI CAGTG 3 cut(s) 120, 494, 1225
Cac8I GCNNGC 1 cut(s) 1332
CaiI CAGNNNCTG 1 cut(s) 938
CciI TCATGA 1 cut(s) 40
CfoI GCGC 1 cut(s) 990
Cfr10I RCCGGY 1 cut(s) 901
Csp6I GTAC 3 cut(s) 1152, 1159, 1203
CspAI ACCGGT 1 cut(s) 901
CviQI GTAC 3 cut(s) 1152, 1159, 1203
DdeI CTNAG 2 cut(s) 324, 1335
DpnI GATC 6 cut(s) 84, 429, 437, 623, 657, 882
DpnII GATC 6 cut(s) 82, 427, 435, 621, 655, 880
DraI TTTAAA 1 cut(s) 718
Eam1104I CTCTTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 706
Ecl136II GAGCTC 1 cut(s) 699
Eco130I CCWWGG 2 cut(s) 670, 1112
Eco24I GRGCYC 1 cut(s) 701
Eco31I GGTCTC 1 cut(s) 510
Eco47III AGCGCT 1 cut(s) 989
Eco53kI GAGCTC 1 cut(s) 699
Eco57I CTGAAG 3 cut(s) 45, 470, 960
Eco88I CYCGRG 1 cut(s) 1325
Eco91I GGTNACC 1 cut(s) 1270
EcoICRI GAGCTC 1 cut(s) 699
EcoNI CCTNNNNNAGG 1 cut(s) 27
EcoO65I GGTNACC 1 cut(s) 1270
EcoT14I CCWWGG 2 cut(s) 670, 1112
EcoT22I ATGCAT 2 cut(s) 644, 1083
EcoT38I GRGCYC 1 cut(s) 701
ErhI CCWWGG 2 cut(s) 670, 1112
Esp3I CGTCTC 1 cut(s) 944
FaqI GGGAC 2 cut(s) 1035, 1094
FblI GTMKAC 2 cut(s) 792, 1169
Fnu4HI GCNGC 2 cut(s) 1090, 1235
FokI GGATG 8 cut(s) 59, 169, 205, 250, 403, 467, 614, 1129
FriOI GRGCYC 1 cut(s) 701
Fsp4HI GCNGC 2 cut(s) 1090, 1235
FspBI CTAG 4 cut(s) 72, 113, 561, 1106
GlaI GCGC 1 cut(s) 989
GluI GCNGC 2 cut(s) 1090, 1235
GsuI CTGGAG 1 cut(s) 817
HaeII RGCGCY 1 cut(s) 991
HaeIII GGCC 1 cut(s) 1013
HapII CCGG 1 cut(s) 902
HhaI GCGC 1 cut(s) 990
Hin6I GCGC 1 cut(s) 988
HinP1I GCGC 1 cut(s) 988
HinfI GANTC 8 cut(s) 66, 271, 532, 605, 739, 844, 855, 1038
HpaII CCGG 1 cut(s) 902
HphI GGTGA 1 cut(s) 779
Hpy166II GTNNAC 6 cut(s) 20, 793, 973, 1152, 1170, 1367
Hpy188I TCNGA 7 cut(s) 738, 843, 849, 896, 1133, 1186, 1338
Hpy188III TCNNGA 8 cut(s) 41, 80, 268, 375, 431, 659, 1007, 1287
Hpy8I GTNNAC 6 cut(s) 20, 793, 973, 1152, 1170, 1367
HpyAV CCTTC 4 cut(s) 20, 445, 494, 1012
HpyCH4III ACNGT 2 cut(s) 1026, 1432
HpyF3I CTNAG 2 cut(s) 324, 1335
HspAI GCGC 1 cut(s) 988
Kzo9I GATC 6 cut(s) 82, 427, 435, 621, 655, 880
LguI GCTCTTC 1 cut(s) 706
LmnI GCTCC 2 cut(s) 577, 844
Lsp1109I GCAGC 2 cut(s) 1101, 1246
LweI GCATC 3 cut(s) 103, 381, 447
MaeI CTAG 4 cut(s) 72, 113, 561, 1106
MaeIII GTNAC 1 cut(s) 1270
MalI GATC 6 cut(s) 84, 429, 437, 623, 657, 882
MboI GATC 6 cut(s) 82, 427, 435, 621, 655, 880
MboII GAAGA 7 cut(s) 68, 75, 693, 926, 953, 1146, 1357
MflI RGATCY 3 cut(s) 435, 621, 655
MhlI GDGCHC 1 cut(s) 701
MlyI GAGTC 2 cut(s) 614, 733
MmeI TCCRAC 1 cut(s) 901
Mph1103I ATGCAT 2 cut(s) 644, 1083
MroXI GAANNNNTTC 1 cut(s) 854
MseI TTAA 5 cut(s) 717, 756, 1098, 1263, 1389
MslI CAYNNNNRTG 3 cut(s) 45, 223, 1433
MspI CCGG 1 cut(s) 902
Mva1269I GAATGC 1 cut(s) 1143
NcoI CCATGG 2 cut(s) 670, 1112
NdeII GATC 6 cut(s) 82, 427, 435, 621, 655, 880
NlaIV GGNNCC 1 cut(s) 318
NmeAIII GCCGAG 1 cut(s) 188
NsiI ATGCAT 2 cut(s) 644, 1083
NspI RCATGY 3 cut(s) 971, 1209, 1334
NspV TTCGAA 1 cut(s) 1421
OliI CACNNNNGTG 1 cut(s) 1433
PaeI GCATGC 1 cut(s) 1334
PaeR7I CTCGAG 1 cut(s) 1325
PagI TCATGA 1 cut(s) 40
PciI ACATGT 1 cut(s) 1205
PciSI GCTCTTC 1 cut(s) 706
PcsI WCGNNNNNNNCGW 1 cut(s) 1063
PctI GAATGC 1 cut(s) 1143
PdmI GAANNNNTTC 1 cut(s) 854
PfeI GAWTC 6 cut(s) 66, 271, 532, 844, 855, 1038
PflFI GACNNNGTC 1 cut(s) 1106
PinAI ACCGGT 1 cut(s) 901
PkrI GCNGC 2 cut(s) 1091, 1236
PleI GAGTC 2 cut(s) 613, 733
PpsI GAGTC 2 cut(s) 613, 733
PscI ACATGT 1 cut(s) 1205
Psp124BI GAGCTC 1 cut(s) 701
PspEI GGTNACC 1 cut(s) 1270
PspN4I GGNNCC 1 cut(s) 318
PspXI VCTCGAGB 1 cut(s) 1325
PstI CTGCAG 1 cut(s) 1377
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 3 cut(s) 435, 621, 655
PsyI GACNNNGTC 1 cut(s) 1106
RsaI GTAC 3 cut(s) 1153, 1160, 1204
RsaNI GTAC 3 cut(s) 1152, 1159, 1203
RseI CAYNNNNRTG 3 cut(s) 45, 223, 1433
SacI GAGCTC 1 cut(s) 701
SapI GCTCTTC 1 cut(s) 706
SaqAI TTAA 5 cut(s) 717, 756, 1098, 1263, 1389
SatI GCNGC 2 cut(s) 1090, 1235
Sau3AI GATC 6 cut(s) 82, 427, 435, 621, 655, 880
SchI GAGTC 2 cut(s) 614, 733
SduI GDGCHC 1 cut(s) 701
SfaNI GCATC 3 cut(s) 103, 381, 447
SfcI CTRYAG 1 cut(s) 1373
Sfr274I CTCGAG 1 cut(s) 1325
SfuI TTCGAA 1 cut(s) 1421
SlaI CTCGAG 1 cut(s) 1325
SmiI ATTTAAAT 1 cut(s) 718
SmiMI CAYNNNNRTG 3 cut(s) 45, 223, 1433
SmlI CTYRAG 4 cut(s) 431, 566, 1256, 1325
SmoI CTYRAG 4 cut(s) 431, 566, 1256, 1325
SpeI ACTAGT 1 cut(s) 71
SphI GCATGC 1 cut(s) 1334
SspI AATATT 1 cut(s) 597
SspMI CTAG 4 cut(s) 72, 113, 561, 1106
SstI GAGCTC 1 cut(s) 701
StyI CCWWGG 2 cut(s) 670, 1112
SwaI ATTTAAAT 1 cut(s) 718
TaaI ACNGT 2 cut(s) 1026, 1432
TaqI TCGA 3 cut(s) 1326, 1421, 1450
TatI WGTACW 1 cut(s) 1158
TfiI GAWTC 6 cut(s) 66, 271, 532, 844, 855, 1038
Tru1I TTAA 5 cut(s) 717, 756, 1098, 1263, 1389
Tru9I TTAA 5 cut(s) 717, 756, 1098, 1263, 1389
TscAI CASTG 3 cut(s) 127, 494, 1232
TseI GCWGC 2 cut(s) 1089, 1234
TspDTI ATGAA 9 cut(s) 213, 254, 263, 354, 469, 524, 536, 798, 1031
TspGWI ACGGA 2 cut(s) 250, 968
TspRI CASTG 3 cut(s) 127, 494, 1232
Tth111I GACNNNGTC 1 cut(s) 1106
XagI CCTNNNNNAGG 1 cut(s) 27
XapI RAATTY 7 cut(s) 165, 200, 241, 628, 719, 871, 1339
XceI RCATGY 3 cut(s) 971, 1209, 1334
XhoI CTCGAG 1 cut(s) 1325
XmiI GTMKAC 2 cut(s) 792, 1169
XmnI GAANNNNTTC 1 cut(s) 854
XspI CTAG 4 cut(s) 72, 113, 561, 1106
Zsp2I ATGCAT 2 cut(s) 644, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.