Rmu_sc0021522.1_g000001

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021522.1
OG0001174
Physical Location & Seq
Forward (+)
1052 .. 1756
705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021522.1_g000001.1.cds

Sequence Viewer

Length: 423 bp
atggagataaggctacatgtgacgaacttgggatcacttcgattgcctattcacctattgctcaaggtacagtgtgaatttttgatgttttgcattccttttagtaatgccttttctgacatgttgctgcaggaatataaggagaacccagattatgcacgttgtgttgctattgtagcatctgcatctttgagggagatgataaaacctggtgccttgggctattatttcacctatagcagttggtattacattctgaatatttggatactatccggaggcaaaggaagtgattgtcataagggtgcaattacaggagacatagtgggagacccattcaaagacacagctggaccttcacttcatgtccttattaaaatgcttgctacaaaaatggtgctcatggctcctgtctttctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

140

Amino Acids

15.67

Weight (kDa)

7.68

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 275
AclWI GGATC 1 cut(s) 40
AcsI RAATTY 1 cut(s) 77
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 1 cut(s) 69
AflIII ACRYGT 2 cut(s) 16, 120
AgsI TTSAA 1 cut(s) 340
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 1 cut(s) 350
AluI AGCT 1 cut(s) 350
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 2 cut(s) 312, 324
AlwI GGATC 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 2 cut(s) 44, 223
AvaII GGWCC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 210
BciVI GTATCC 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 312, 324
BfmI CTRYAG 2 cut(s) 128, 235
BfuI GTATCC 1 cut(s) 261
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 210
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 214, 408
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 2 cut(s) 188, 194
BpuEI CTTGAG 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
BseAI TCCGGA 1 cut(s) 275
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 216
BseXI GCAGC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 1 cut(s) 276
BsmAI GTCTC 2 cut(s) 312, 324
BsmI GAATGC 1 cut(s) 93
Bso31I GGTCTC 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 402
Bsp13I TCCGGA 1 cut(s) 275
Bsp143I GATC 1 cut(s) 32
BspEI TCCGGA 1 cut(s) 275
BspLI GGNNCC 2 cut(s) 214, 408
BspMAI CTGCAG 1 cut(s) 132
BspPI GGATC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 324
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 384
BstKTI GATC 1 cut(s) 35
BstMAI GTCTC 2 cut(s) 312, 324
BstMBI GATC 1 cut(s) 32
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 1 cut(s) 210
BstNSI RCATGY 2 cut(s) 20, 124
BstSCI CCNGG 1 cut(s) 208
BstSFI CTRYAG 2 cut(s) 128, 235
BstV1I GCAGC 1 cut(s) 114
BsuI GTATCC 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 384
Cfr13I GGNCC 1 cut(s) 353
CsiI ACCWGGT 1 cut(s) 208
Csp6I GTAC 1 cut(s) 68
CviAII CATG 4 cut(s) 17, 121, 365, 403
CviJI RGCY 4 cut(s) 13, 222, 350, 407
CviKI_1 RGCY 4 cut(s) 13, 222, 350, 407
CviQI GTAC 1 cut(s) 68
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
DraIII CACNNNGTG 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 4 cut(s) 20, 124, 368, 406
FaiI YATR 9 cut(s) 18, 122, 138, 156, 237, 300, 323, 366, 404
FatI CATG 4 cut(s) 16, 120, 364, 402
Fnu4HI GCNGC 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 128
HapII CCGG 1 cut(s) 276
Hin1II CATG 4 cut(s) 20, 124, 368, 406
HpaII CCGG 1 cut(s) 276
HphI GGTGA 2 cut(s) 44, 223
Hpy188I TCNGA 3 cut(s) 118, 258, 422
Hpy188III TCNNGA 1 cut(s) 276
HpyAV CCTTC 1 cut(s) 366
HpyCH4III ACNGT 1 cut(s) 72
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 5 cut(s) 93, 130, 158, 185, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 4 cut(s) 20, 124, 368, 406
Kpn2I TCCGGA 1 cut(s) 275
Kzo9I GATC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 7 cut(s) 116, 162, 195, 222, 289, 300, 336
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 2 cut(s) 188, 194
MabI ACCWGGT 1 cut(s) 208
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 1 cut(s) 19
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 2 cut(s) 77, 309
MnlI CCTC 2 cut(s) 186, 272
MroI TCCGGA 1 cut(s) 275
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 1 cut(s) 210
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 176
NdeII GATC 1 cut(s) 32
NlaIII CATG 4 cut(s) 20, 124, 368, 406
NlaIV GGNNCC 2 cut(s) 214, 408
NmuCI GTSAC 1 cut(s) 19
NspI RCATGY 2 cut(s) 20, 124
PciI ACATGT 2 cut(s) 16, 120
PctI GAATGC 1 cut(s) 93
PkrI GCNGC 1 cut(s) 129
PscI ACATGT 2 cut(s) 16, 120
Psp6I CCWGG 1 cut(s) 208
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 2 cut(s) 214, 408
PspPI GGNCC 1 cut(s) 353
PstI CTGCAG 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 1 cut(s) 32
Sau96I GGNCC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 1 cut(s) 402
SetI ASST 7 cut(s) 57, 69, 163, 211, 236, 352, 358
SexAI ACCWGGT 1 cut(s) 208
SfaNI GCATC 2 cut(s) 188, 194
SfcI CTRYAG 2 cut(s) 128, 235
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 2 cut(s) 77, 309
SspI AATATT 1 cut(s) 262
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 72
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 40
TasI AATT 2 cut(s) 77, 309
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 1 cut(s) 353
TspRI CASTG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 77
XceI RCATGY 2 cut(s) 20, 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.