Rmu_sc0007727.1_g000033

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007727.1
OG0001174
Physical Location & Seq
Forward (+)
157043 .. 157619
577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007727.1_g000033.1.cds

Sequence Viewer

Length: 342 bp
atggagataaggctacatgtgacgaacttgggatcacttcgattgcctattcacctattgctcaaggtacagtgtgaatttttgatgttttgcattccttttagtaatgccttttctgacatgttgctgcaggaatataaggagaacccagattatgcacgttgtgttgctattgtagcatctgcatctttgagggagatgataaaacctggtgccttggctattatttcacctatagcagttggtgggtattacattctgaatatttggatactatccggaggcaaaggaagtgattgtcataagggtgcaattacaggagacatggctttccctccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

113

Amino Acids

12.4

Weight (kDa)

6.8

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 278
AclWI GGATC 1 cut(s) 40
AcsI RAATTY 1 cut(s) 77
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 1 cut(s) 69
AflIII ACRYGT 2 cut(s) 16, 120
AjnI CCWGG 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 44, 222
BanI GGYRCC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 210
BciVI GTATCC 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 315
BfmI CTRYAG 2 cut(s) 128, 234
BfuI GTATCC 1 cut(s) 264
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 2 cut(s) 188, 194
BpuEI CTTGAG 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 278
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 216
BseXI GCAGC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 212
BsiSI CCGG 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 93
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 1 cut(s) 32
BspEI TCCGGA 1 cut(s) 278
BspLI GGNNCC 1 cut(s) 214
BspMAI CTGCAG 1 cut(s) 132
BspPI GGATC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 72
BstKTI GATC 1 cut(s) 35
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 1 cut(s) 32
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 1 cut(s) 210
BstNSI RCATGY 2 cut(s) 20, 124
BstSCI CCNGG 1 cut(s) 208
BstSFI CTRYAG 2 cut(s) 128, 234
BstV1I GCAGC 1 cut(s) 114
BsuI GTATCC 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 77
CsiI ACCWGGT 1 cut(s) 208
Csp6I GTAC 1 cut(s) 68
CviAII CATG 3 cut(s) 17, 121, 325
CviJI RGCY 3 cut(s) 13, 221, 329
CviKI_1 RGCY 3 cut(s) 13, 221, 329
CviQI GTAC 1 cut(s) 68
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
DraIII CACNNNGTG 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 3 cut(s) 20, 124, 328
FaiI YATR 7 cut(s) 18, 122, 138, 156, 236, 303, 326
FatI CATG 3 cut(s) 16, 120, 324
Fnu4HI GCNGC 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 128
HapII CCGG 1 cut(s) 279
Hin1II CATG 3 cut(s) 20, 124, 328
HpaII CCGG 1 cut(s) 279
HphI GGTGA 2 cut(s) 44, 222
Hpy188I TCNGA 2 cut(s) 118, 261
Hpy188III TCNNGA 1 cut(s) 279
HpyCH4III ACNGT 1 cut(s) 72
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 5 cut(s) 93, 130, 158, 185, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 3 cut(s) 20, 124, 328
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 1 cut(s) 32
LpnPI CCDG 6 cut(s) 116, 162, 195, 222, 292, 303
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 2 cut(s) 188, 194
MabI ACCWGGT 1 cut(s) 208
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 1 cut(s) 19
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MluCI AATT 2 cut(s) 77, 312
MnlI CCTC 2 cut(s) 186, 275
MroI TCCGGA 1 cut(s) 278
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 1 cut(s) 210
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 176
NdeII GATC 1 cut(s) 32
NlaIII CATG 3 cut(s) 20, 124, 328
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 19
NspI RCATGY 2 cut(s) 20, 124
PciI ACATGT 2 cut(s) 16, 120
PctI GAATGC 1 cut(s) 93
PkrI GCNGC 1 cut(s) 129
PscI ACATGT 2 cut(s) 16, 120
Psp6I CCWGG 1 cut(s) 208
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 214
PstI CTGCAG 1 cut(s) 132
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 306
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 210
SetI ASST 5 cut(s) 57, 69, 163, 211, 235
SexAI ACCWGGT 1 cut(s) 208
SfaNI GCATC 2 cut(s) 188, 194
SfcI CTRYAG 2 cut(s) 128, 234
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 2 cut(s) 77, 312
SspI AATATT 1 cut(s) 265
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 72
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 40
TasI AATT 2 cut(s) 77, 312
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 19
TspRI CASTG 1 cut(s) 77
XapI RAATTY 1 cut(s) 77
XceI RCATGY 2 cut(s) 20, 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.