Rh3AG230800

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001174
Physical Location & Seq
Forward (+)
23827849 .. 23829403
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG230800.1

Sequence Viewer

Length: 516 bp
ATGTATCACTGTTTCATGATCGAGGAAGAAGTGGAGCTTGTGTGGCCACTAATTGACATAAAAAAGACTGGACAAGTCGATAGGAAAACTCTAACAAAGTGGGTGACAGGTATCCCACTAGCATCAAAGCAAGTGAATTATAGTCTCATATACAAGGCACTAGAGGAGAATGGAGATAAGGCTACATGTGACGAACTTGGGATCACTTCGATTGCCTATTCACCTATTGCTCAAGGAGAACCCAGATTATGCACGTTGTGTTGCTATTGTAGCATCTGCATCTTTGAGGGAGATGATAAAACCTGGTGCCTTGGCTATTATTTCACCTATAGCAGTTGGTATTGCGTTCTGAATATTTGGATACTATCCGGAGGCAAAGGAAGTGATTGTCATAAGGGTGCAATTACAGGAGACATAGTGGGAGACCCATTCAAAGACACAGCTGGACCGTCACTTCATGTCCTTATTAAAATGCTTGCTACAAAAATGGTGGTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

171

Amino Acids

19.07

Weight (kDa)

5.33

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 124 - 165 1.6e-15 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 368
AclWI GGATC 1 cut(s) 209
AcoI YGGCCR 1 cut(s) 44
AdeI CACNNNGTG 1 cut(s) 258
AflIII ACRYGT 1 cut(s) 185
AgsI TTSAA 1 cut(s) 433
AjnI CCWGG 1 cut(s) 302
AluBI AGCT 2 cut(s) 37, 443
AluI AGCT 2 cut(s) 37, 443
Alw26I GTCTC 3 cut(s) 149, 405, 417
AlwI GGATC 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 446
AsuHPI GGTGA 3 cut(s) 115, 213, 316
AvaII GGWCC 1 cut(s) 446
BalI TGGCCA 1 cut(s) 46
BanI GGYRCC 1 cut(s) 306
BciT130I CCWGG 1 cut(s) 304
BciVI GTATCC 2 cut(s) 122, 354
BcoDI GTCTC 3 cut(s) 149, 405, 417
BfaI CTAG 2 cut(s) 119, 161
BfmI CTRYAG 1 cut(s) 328
BfuI GTATCC 2 cut(s) 122, 354
Bme1390I CCNGG 1 cut(s) 304
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 2 cut(s) 308, 501
BmrFI CCNGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 131, 282, 288
BpuEI CTTGAG 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 310
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 402, 432
Bse1I ACTGG 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 368
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 310
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 179
BshFI GGCC 1 cut(s) 46
BshNI GGYRCC 1 cut(s) 306
BsiSI CCGG 1 cut(s) 369
BsmAI GTCTC 3 cut(s) 149, 405, 417
BsnI GGCC 1 cut(s) 46
Bso31I GGTCTC 1 cut(s) 417
Bsp13I TCCGGA 1 cut(s) 368
Bsp143I GATC 2 cut(s) 18, 201
BspANI GGCC 1 cut(s) 46
BspEI TCCGGA 1 cut(s) 368
BspHI TCATGA 1 cut(s) 15
BspLI GGNNCC 2 cut(s) 308, 501
BspPI GGATC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 306
BspTNI GGTCTC 1 cut(s) 417
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 2 cut(s) 18, 201
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 11, 450
BstC8I GCNNGC 1 cut(s) 477
BstKTI GATC 2 cut(s) 21, 204
BstMAI GTCTC 3 cut(s) 149, 405, 417
BstMBI GATC 2 cut(s) 18, 201
BstMWI GCNNNNNNNGC 2 cut(s) 43, 270
BstNI CCWGG 1 cut(s) 304
BstNSI RCATGY 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 302
BstSFI CTRYAG 1 cut(s) 328
BsuI GTATCC 2 cut(s) 122, 354
BsuRI GGCC 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 7
Cac8I GCNNGC 1 cut(s) 477
CciI TCATGA 1 cut(s) 15
Cfr13I GGNCC 1 cut(s) 446
CsiI ACCWGGT 1 cut(s) 302
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviAII CATG 4 cut(s) 16, 186, 458, 496
CviJI RGCY 6 cut(s) 37, 46, 182, 315, 443, 500
CviKI_1 RGCY 6 cut(s) 37, 46, 182, 315, 443, 500
DpnI GATC 2 cut(s) 20, 203
DpnII GATC 2 cut(s) 18, 201
DraIII CACNNNGTG 1 cut(s) 258
EaeI YGGCCR 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 4 cut(s) 19, 189, 461, 499
FalI AAGNNNNNCTT 2 cut(s) 21, 53
FatI CATG 4 cut(s) 15, 185, 457, 495
FspBI CTAG 2 cut(s) 119, 161
HaeIII GGCC 1 cut(s) 46
HapII CCGG 1 cut(s) 369
Hin1II CATG 4 cut(s) 19, 189, 461, 499
HpaII CCGG 1 cut(s) 369
HphI GGTGA 3 cut(s) 115, 213, 316
Hpy188I TCNGA 2 cut(s) 351, 515
Hpy188III TCNNGA 2 cut(s) 16, 369
HpyCH4III ACNGT 2 cut(s) 11, 450
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 3 cut(s) 252, 279, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 270
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 4 cut(s) 19, 189, 461, 499
Kpn2I TCCGGA 1 cut(s) 368
Kzo9I GATC 2 cut(s) 18, 201
LmnI GCTCC 2 cut(s) 34, 505
LpnPI CCDG 8 cut(s) 54, 93, 256, 289, 316, 382, 393, 429
LweI GCATC 3 cut(s) 131, 282, 288
MabI ACCWGGT 1 cut(s) 302
MaeI CTAG 2 cut(s) 119, 161
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 3 cut(s) 103, 188, 450
MalI GATC 2 cut(s) 20, 203
MboI GATC 2 cut(s) 18, 201
MboII GAAGA 1 cut(s) 38
MlsI TGGCCA 1 cut(s) 46
MluCI AATT 3 cut(s) 51, 136, 402
MluNI TGGCCA 1 cut(s) 46
MnlI CCTC 4 cut(s) 16, 157, 280, 365
Mox20I TGGCCA 1 cut(s) 46
MroI TCCGGA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 46
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 1 cut(s) 396
Msp20I TGGCCA 1 cut(s) 46
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 1 cut(s) 304
MvaI CCWGG 1 cut(s) 304
MwoI GCNNNNNNNGC 2 cut(s) 43, 270
NdeII GATC 2 cut(s) 18, 201
NlaIII CATG 4 cut(s) 19, 189, 461, 499
NlaIV GGNNCC 2 cut(s) 308, 501
NmuCI GTSAC 3 cut(s) 103, 188, 450
NspI RCATGY 1 cut(s) 189
PagI TCATGA 1 cut(s) 15
PciI ACATGT 1 cut(s) 185
PscI ACATGT 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 302
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 308, 501
PspPI GGNCC 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 443
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 1 cut(s) 468
Sau3AI GATC 2 cut(s) 18, 201
Sau96I GGNCC 1 cut(s) 446
ScrFI CCNGG 1 cut(s) 304
SetI ASST 7 cut(s) 39, 112, 226, 257, 305, 329, 445
SexAI ACCWGGT 1 cut(s) 302
SfaNI GCATC 3 cut(s) 131, 282, 288
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
Sse9I AATT 3 cut(s) 51, 136, 402
SspI AATATT 1 cut(s) 355
SspMI CTAG 2 cut(s) 119, 161
StyD4I CCNGG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 2 cut(s) 11, 450
TaiI ACGT 1 cut(s) 257
TaqI TCGA 3 cut(s) 21, 78, 209
TasI AATT 3 cut(s) 51, 136, 402
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 1 cut(s) 14
TseFI GTSAC 3 cut(s) 103, 188, 450
Tsp45I GTSAC 3 cut(s) 103, 188, 450
TspDTI ATGAA 2 cut(s) 4, 446
TspRI CASTG 1 cut(s) 14
VpaK11BI GGWCC 1 cut(s) 446
XceI RCATGY 1 cut(s) 189
XspI CTAG 2 cut(s) 119, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.