Rw4G008580

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001174
Physical Location & Seq
Forward (+)
18868579 .. 18870222
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008580.1

Sequence Viewer

Length: 321 bp
ATGGACAAGGACAAGAGGCGTACCGATCTGCTCGCTGCTGGCCGTAAAAAGGAATATAAGGAGAAGCCAGATTATGCACGTTGTGTTGCTATTGTAGCATCTACATCTTTGAGGGAGATGATAAAACCTAGTGCCTTGGCTATTATTTCACCTATAGCAGTTGGTATTGCATTCCGGATATTTGGATACTATCCGGAGGCAAAGGAAGTGACTGTCATAAGAGTGGGAGACCCATTCAAAGACACAGCTAGACCTTCACTTCATGTCCTTATTAAAATGCTTGCTACAAAAATGCTGGTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

106

Amino Acids

11.85

Weight (kDa)

9.83

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 18 - 59 1e-09 Inorganic H+ pyrophosphatase
H_PPase PF03030 74 - 101 7.6e-07 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 174, 193
AcoI YGGCCR 1 cut(s) 40
AdeI CACNNNGTG 1 cut(s) 83
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 1 cut(s) 238
AluBI AGCT 1 cut(s) 248
AluI AGCT 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 222
Aor13HI TCCGGA 2 cut(s) 174, 193
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 141
BbvI GCAGC 1 cut(s) 22
BceAI ACGGC 1 cut(s) 27
BciVI GTATCC 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 222
BfaI CTAG 2 cut(s) 129, 249
BfmI CTRYAG 1 cut(s) 153
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 306
BmsI GCATC 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 2 cut(s) 174, 193
Bsc4I CCNNNNNNNGG 1 cut(s) 49
BseAI TCCGGA 2 cut(s) 174, 193
BseDI CCNNGG 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 22
BshFI GGCC 1 cut(s) 42
BsiSI CCGG 2 cut(s) 175, 194
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 1 cut(s) 42
Bso31I GGTCTC 1 cut(s) 222
Bsp13I TCCGGA 2 cut(s) 174, 193
Bsp143I GATC 1 cut(s) 25
BspANI GGCC 1 cut(s) 42
BspEI TCCGGA 2 cut(s) 174, 193
BspLI GGNNCC 1 cut(s) 306
BspTNI GGTCTC 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 214
BstC8I GCNNGC 3 cut(s) 33, 40, 282
BstKTI GATC 1 cut(s) 28
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 1 cut(s) 25
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 22
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 1 cut(s) 42
Cac8I GCNNGC 3 cut(s) 33, 40, 282
Csp6I GTAC 1 cut(s) 21
CviAII CATG 2 cut(s) 263, 301
CviJI RGCY 5 cut(s) 42, 67, 140, 248, 305
CviKI_1 RGCY 5 cut(s) 42, 67, 140, 248, 305
CviQI GTAC 1 cut(s) 21
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
DraIII CACNNNGTG 1 cut(s) 83
EaeI YGGCCR 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 2 cut(s) 266, 304
FaiI YATR 6 cut(s) 57, 75, 155, 218, 264, 302
FatI CATG 2 cut(s) 262, 300
Fnu4HI GCNGC 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 2 cut(s) 129, 249
GluI GCNGC 1 cut(s) 36
HaeIII GGCC 1 cut(s) 42
HapII CCGG 2 cut(s) 175, 194
Hin1II CATG 2 cut(s) 266, 304
HpaII CCGG 2 cut(s) 175, 194
HphI GGTGA 1 cut(s) 141
Hpy188I TCNGA 1 cut(s) 320
Hpy188III TCNNGA 2 cut(s) 175, 194
HpyAV CCTTC 1 cut(s) 264
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 2 cut(s) 77, 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 2 cut(s) 266, 304
Kpn2I TCCGGA 2 cut(s) 174, 193
Kzo9I GATC 1 cut(s) 25
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 5 cut(s) 24, 81, 188, 207, 281
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 107
MaeI CTAG 2 cut(s) 129, 249
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 208
MalI GATC 1 cut(s) 27
MboI GATC 1 cut(s) 25
MnlI CCTC 3 cut(s) 9, 105, 190
MroI TCCGGA 2 cut(s) 174, 193
MseI TTAA 1 cut(s) 273
MslI CAYNNNNRTG 1 cut(s) 221
MspI CCGG 2 cut(s) 175, 194
Mva1269I GAATGC 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 95
NdeII GATC 1 cut(s) 25
NlaIII CATG 2 cut(s) 266, 304
NlaIV GGNNCC 1 cut(s) 306
NmuCI GTSAC 1 cut(s) 208
PctI GAATGC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 306
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 1 cut(s) 25
SetI ASST 5 cut(s) 82, 130, 154, 250, 256
SfaNI GCATC 1 cut(s) 107
SfcI CTRYAG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 221
SspMI CTAG 2 cut(s) 129, 249
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 82
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 251
XspI CTAG 2 cut(s) 129, 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.