Rh4AG105800

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001174
Physical Location & Seq
Forward (+)
22810313 .. 22812468
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG105800.1

Sequence Viewer

Length: 525 bp
ATGAAGAATAAAACCCCAGCCACCCCTCCATCGATAGAGATCATCGGACCAAGGGAAAACGAAGGTTGGGTTGGATCTAAGGGAAGCAACCACACGTCCCCACAGTCCCAACCAGAGACAAGAGAGAAGAGAAAGAGAGAATGGCCTAAGAGACTAGCAATTAAGCAATTCGCACCAAAACCACAAGATCGAGCTGGTGCTAAATCCAGGTGGAGAAAGCGGAGTTTAATGGAATATAAGGAGAAGCCAGATTATGCACATTGTGTTGCTATTGTAGCATCTGCATCTTTGAGGGAGATGATAAAACCTGGTGCCATGGCTATTATTTCACCTATAGCAGTTGGTGGGTATTGCATTCCGGATATTTGGATACTATCCGGAGGCAAAGGAAGTAACTGTCATAAGGCTGCAATTACAGGAGACATAGTGGGAGACCCATTCAAAGACACAGCTAGACCTTCACTTCATGTCCTTATTAAAATGCTTGCTACAAAAATGCTGGTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

174

Amino Acids

19.17

Weight (kDa)

9.97

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 73 - 116 4.7e-10 Inorganic H+ pyrophosphatase
H_PPase PF03030 127 - 169 1.9e-15 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 311
AccIII TCCGGA 2 cut(s) 358, 377
AciI CCGC 1 cut(s) 220
AclWI GGATC 1 cut(s) 82
AdeI CACNNNGTG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 1 cut(s) 442
AjiI CACGTC 1 cut(s) 96
AjnI CCWGG 2 cut(s) 206, 307
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 2 cut(s) 194, 452
AluI AGCT 2 cut(s) 194, 452
Alw26I GTCTC 4 cut(s) 110, 145, 414, 426
AlwI GGATC 1 cut(s) 82
Aor13HI TCCGGA 2 cut(s) 358, 377
AoxI GGCC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 321
AvaII GGWCC 1 cut(s) 47
BanI GGYRCC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 394
BccI CCATC 1 cut(s) 37
BciT130I CCWGG 2 cut(s) 208, 309
BciVI GTATCC 1 cut(s) 363
BcoDI GTCTC 4 cut(s) 110, 145, 414, 426
BfaI CTAG 2 cut(s) 155, 453
BfmI CTRYAG 1 cut(s) 333
BfuI GTATCC 1 cut(s) 363
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
Bme1390I CCNGG 2 cut(s) 208, 309
Bme18I GGWCC 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 2 cut(s) 313, 510
BmrFI CCNGG 2 cut(s) 208, 309
BmsI GCATC 2 cut(s) 287, 293
Bsa29I ATCGAT 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 50, 315
BsaWI WCCGGW 2 cut(s) 358, 377
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bse8I GATNNNNATC 1 cut(s) 38
BseAI TCCGGA 2 cut(s) 358, 377
BseBI CCWGG 2 cut(s) 208, 309
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 50, 315
BseJI GATNNNNATC 1 cut(s) 38
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 145
BshNI GGYRCC 1 cut(s) 311
BshVI ATCGAT 1 cut(s) 32
BsiSI CCGG 2 cut(s) 359, 378
BslFI GGGAC 2 cut(s) 82, 91
BsmAI GTCTC 4 cut(s) 110, 145, 414, 426
BsmFI GGGAC 2 cut(s) 82, 91
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 1 cut(s) 145
Bso31I GGTCTC 1 cut(s) 426
Bsp13I TCCGGA 2 cut(s) 358, 377
Bsp143I GATC 3 cut(s) 39, 74, 187
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 1 cut(s) 220
BspANI GGCC 1 cut(s) 145
BspDI ATCGAT 1 cut(s) 32
BspEI TCCGGA 2 cut(s) 358, 377
BspLI GGNNCC 2 cut(s) 313, 510
BspPI GGATC 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 426
BssECI CCNNGG 2 cut(s) 50, 315
BssMI GATC 3 cut(s) 39, 74, 187
BssT1I CCWWGG 2 cut(s) 50, 315
Bst2UI CCWGG 2 cut(s) 208, 309
Bst4CI ACNGT 2 cut(s) 105, 398
Bst6I CTCTTC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 2 cut(s) 78, 147
BstDSI CCRYGG 1 cut(s) 315
BstKTI GATC 3 cut(s) 42, 77, 190
BstMAI GTCTC 4 cut(s) 110, 145, 414, 426
BstMBI GATC 3 cut(s) 39, 74, 187
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNI CCWGG 2 cut(s) 208, 309
BstSCI CCNGG 2 cut(s) 206, 307
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 363
BsuRI GGCC 1 cut(s) 145
BsuTUI ATCGAT 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 315
BtrI CACGTC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 47
ClaI ATCGAT 1 cut(s) 32
CsiI ACCWGGT 1 cut(s) 307
CviAII CATG 3 cut(s) 316, 467, 505
CviJI RGCY 8 cut(s) 20, 145, 194, 247, 320, 407, 452, 509
CviKI_1 RGCY 8 cut(s) 20, 145, 194, 247, 320, 407, 452, 509
DdeI CTNAG 2 cut(s) 78, 147
DpnI GATC 3 cut(s) 41, 76, 189
DpnII GATC 3 cut(s) 39, 74, 187
DraIII CACNNNGTG 1 cut(s) 263
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 50, 315
Eco31I GGTCTC 1 cut(s) 426
Eco47I GGWCC 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 206, 307
EcoT14I CCWWGG 2 cut(s) 50, 315
ErhI CCWWGG 2 cut(s) 50, 315
FaeI CATG 3 cut(s) 319, 470, 508
FaiI YATR 8 cut(s) 237, 255, 317, 335, 402, 425, 468, 506
FaqI GGGAC 2 cut(s) 82, 91
FatI CATG 3 cut(s) 315, 466, 504
Fnu4HI GCNGC 1 cut(s) 408
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 2 cut(s) 155, 453
GluI GCNGC 1 cut(s) 408
GsaI CCCAGC 1 cut(s) 20
HaeIII GGCC 1 cut(s) 145
HapII CCGG 2 cut(s) 359, 378
Hin1II CATG 3 cut(s) 319, 470, 508
HpaII CCGG 2 cut(s) 359, 378
HphI GGTGA 1 cut(s) 321
Hpy188I TCNGA 2 cut(s) 47, 524
Hpy188III TCNNGA 2 cut(s) 359, 378
HpyAV CCTTC 2 cut(s) 56, 468
HpyCH4III ACNGT 2 cut(s) 105, 398
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 4 cut(s) 257, 284, 354, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 2 cut(s) 78, 147
HpySE526I ACGT 1 cut(s) 95
Hsp92II CATG 3 cut(s) 319, 470, 508
Kpn2I TCCGGA 2 cut(s) 358, 377
Kzo9I GATC 3 cut(s) 39, 74, 187
LmnI GCTCC 1 cut(s) 514
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 2 cut(s) 287, 293
MabI ACCWGGT 1 cut(s) 307
MaeI CTAG 2 cut(s) 155, 453
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 392
MalI GATC 3 cut(s) 41, 76, 189
MboI GATC 3 cut(s) 39, 74, 187
MboII GAAGA 2 cut(s) 16, 139
MflI RGATCY 1 cut(s) 74
MluCI AATT 3 cut(s) 159, 167, 411
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 3 cut(s) 36, 285, 374
MroI TCCGGA 2 cut(s) 358, 377
MseI TTAA 3 cut(s) 162, 227, 477
MspI CCGG 2 cut(s) 359, 378
MspR9I CCNGG 2 cut(s) 208, 309
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 2 cut(s) 208, 309
MwoI GCNNNNNNNGC 1 cut(s) 275
NcoI CCATGG 1 cut(s) 315
NdeII GATC 3 cut(s) 39, 74, 187
NlaIII CATG 3 cut(s) 319, 470, 508
NlaIV GGNNCC 2 cut(s) 313, 510
PctI GAATGC 1 cut(s) 354
PkrI GCNGC 1 cut(s) 409
Psp6I CCWGG 2 cut(s) 206, 307
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 2 cut(s) 206, 307
PspN4I GGNNCC 2 cut(s) 313, 510
PspPI GGNCC 1 cut(s) 47
PsuI RGATCY 1 cut(s) 74
SaqAI TTAA 3 cut(s) 162, 227, 477
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 3 cut(s) 39, 74, 187
Sau96I GGNCC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 208, 309
SetI ASST 8 cut(s) 67, 98, 196, 212, 310, 334, 454, 460
SexAI ACCWGGT 1 cut(s) 307
SfaNI GCATC 2 cut(s) 287, 293
SfcI CTRYAG 1 cut(s) 333
SinI GGWCC 1 cut(s) 47
Sse9I AATT 3 cut(s) 159, 167, 411
SsiI CCGC 1 cut(s) 220
SspMI CTAG 2 cut(s) 155, 453
StyD4I CCNGG 2 cut(s) 206, 307
StyI CCWWGG 2 cut(s) 50, 315
TaaI ACNGT 2 cut(s) 105, 398
TaiI ACGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 32, 190
TasI AATT 3 cut(s) 159, 167, 411
Tru1I TTAA 3 cut(s) 162, 227, 477
Tru9I TTAA 3 cut(s) 162, 227, 477
TseI GCWGC 1 cut(s) 407
TspDTI ATGAA 2 cut(s) 17, 455
VpaK11BI GGWCC 1 cut(s) 47
XspI CTAG 2 cut(s) 155, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.