Rorug05G0301400

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001174
Physical Location & Seq
Reverse (-)
34992021 .. 34993441
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0301400.1

Sequence Viewer

Length: 456 bp
ATGGAAGCCTCGATCTCCTCCTTCACACTCATCAGAACCCCAACCAAGTCCAACTCCAGCACAGCTATATTTCCAAGCATTAACATCACAAAACCATATGATTCATGCTCTATTACCAGAACAAAATGCCACAACCTCGCTTTTGCTATAAACACTACTACTCCAACCCTCCACGGCAGAAAAGCTTGGAAGACTGCAGCTATCGAAGATGTTTCCGGCGCTATAACGGATCCGGGACCGGTCGTCCTCACCTGGCAAATTGTCGTCGGAGCTATAGCTGGAGTTACACCTTTTGTGGTAGCAGGGATTGAATTCAGCAAAAGAATTATAGCACAACAGAGATGTGAGGTGTGTGGAGGGTCAGGACTTGTTTTGACTAAAGAAAACTATATGAAATGTCCTGGATGCGGTGGATTTCTCCCTTGGCAAACATGGAAAAGATTCTTTACTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

151

Amino Acids

16.29

Weight (kDa)

9.33

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 408
AclWI GGATC 2 cut(s) 224, 237
AcsI RAATTY 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 407
AgeI ACCGGT 1 cut(s) 238
AgsI TTSAA 1 cut(s) 311
AhdI GACNNNNNGTC 1 cut(s) 242
AjnI CCWGG 2 cut(s) 251, 400
AluBI AGCT 5 cut(s) 65, 185, 200, 272, 278
AluI AGCT 5 cut(s) 65, 185, 200, 272, 278
AlwI GGATC 2 cut(s) 224, 237
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 1 cut(s) 311
AsiGI ACCGGT 1 cut(s) 238
Asp700I GAANNNNTTC 1 cut(s) 440
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 236
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 236
BamHI GGATCC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 2 cut(s) 253, 402
BcnI CCSGG 1 cut(s) 234
BfmI CTRYAG 2 cut(s) 195, 273
BfoI RGCGCY 1 cut(s) 222
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 3 cut(s) 234, 253, 402
Bme18I GGWCC 1 cut(s) 236
BmeRI GACNNNNNGTC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 2 cut(s) 231, 237
BmrFI CCNGG 3 cut(s) 234, 253, 402
BmsI GCATC 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 40, 300
BpuMI CCSGG 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 172, 422
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 1 cut(s) 454
BseBI CCWGG 2 cut(s) 253, 402
BseDI CCNNGG 2 cut(s) 172, 422
BseGI GGATG 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 407
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 7
BseXI GCAGC 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 243
BshTI ACCGGT 1 cut(s) 238
BsiEI CGRYCG 1 cut(s) 243
BsiSI CCGG 3 cut(s) 216, 233, 239
BslFI GGGAC 1 cut(s) 249
BslI CCNNNNNNNGG 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 12, 229
BspACI CCGC 1 cut(s) 408
BspLI GGNNCC 2 cut(s) 231, 237
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 2 cut(s) 224, 237
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 1 cut(s) 454
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 2 cut(s) 172, 422
BssMI GATC 2 cut(s) 12, 229
BssT1I CCWWGG 1 cut(s) 422
Bst2UI CCWGG 2 cut(s) 253, 402
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 410
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 2 cut(s) 15, 232
BstMBI GATC 2 cut(s) 12, 229
BstMCI CGRYCG 1 cut(s) 243
BstNI CCWGG 2 cut(s) 253, 402
BstSCI CCNGG 3 cut(s) 232, 251, 400
BstSFI CTRYAG 2 cut(s) 195, 273
BstV1I GCAGC 1 cut(s) 209
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 410
CfoI GCGC 1 cut(s) 221
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 236
CspAI ACCGGT 1 cut(s) 238
CviAII CATG 2 cut(s) 105, 432
CviJI RGCY 7 cut(s) 8, 65, 185, 200, 272, 278, 453
CviKI_1 RGCY 7 cut(s) 8, 65, 185, 200, 272, 278, 453
DpnI GATC 2 cut(s) 14, 231
DpnII GATC 2 cut(s) 12, 229
DriI GACNNNNNGTC 1 cut(s) 242
Eam1105I GACNNNNNGTC 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 251, 400
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 2 cut(s) 108, 435
FaqI GGGAC 1 cut(s) 249
FatI CATG 2 cut(s) 104, 431
FauNDI CATATG 1 cut(s) 97
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 1 cut(s) 417
Fsp4HI GCNGC 1 cut(s) 198
GlaI GCGC 1 cut(s) 220
GluI GCNGC 1 cut(s) 198
GsuI CTGGAG 2 cut(s) 40, 300
HaeII RGCGCY 1 cut(s) 222
HapII CCGG 3 cut(s) 216, 233, 239
HhaI GCGC 1 cut(s) 221
Hin1II CATG 2 cut(s) 108, 435
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 2 cut(s) 101, 441
HpaII CCGG 3 cut(s) 216, 233, 239
HphI GGTGA 1 cut(s) 241
Hpy188I TCNGA 2 cut(s) 35, 269
Hpy188III TCNNGA 1 cut(s) 363
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 31
HpyCH4V TGCA 1 cut(s) 197
Hsp92II CATG 2 cut(s) 108, 435
HspAI GCGC 1 cut(s) 219
Kzo9I GATC 2 cut(s) 12, 229
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 1 cut(s) 209
LweI GCATC 1 cut(s) 395
MaeIII GTNAC 1 cut(s) 283
MalI GATC 2 cut(s) 14, 231
MboI GATC 2 cut(s) 12, 229
MboII GAAGA 2 cut(s) 202, 218
MflI RGATCY 1 cut(s) 229
MluCI AATT 3 cut(s) 258, 311, 324
MmeI TCCRAC 3 cut(s) 75, 188, 247
MnlI CCTC 7 cut(s) 19, 28, 146, 179, 257, 340, 350
MroXI GAANNNNTTC 1 cut(s) 440
MseI TTAA 1 cut(s) 81
MspI CCGG 3 cut(s) 216, 233, 239
MspR9I CCNGG 3 cut(s) 234, 253, 402
MvaI CCWGG 2 cut(s) 253, 402
NciI CCSGG 1 cut(s) 234
NdeI CATATG 1 cut(s) 97
NdeII GATC 2 cut(s) 12, 229
NlaIII CATG 2 cut(s) 108, 435
NlaIV GGNNCC 2 cut(s) 231, 237
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 2 cut(s) 101, 441
PfoI TCCNGGA 2 cut(s) 232, 400
PinAI ACCGGT 1 cut(s) 238
PkrI GCNGC 1 cut(s) 199
Psp6I CCWGG 2 cut(s) 251, 400
PspGI CCWGG 2 cut(s) 251, 400
PspN4I GGNNCC 2 cut(s) 231, 237
PspPI GGNCC 1 cut(s) 236
PstI CTGCAG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 229
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 2 cut(s) 12, 229
Sau96I GGNCC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 234, 253, 402
SetI ASST 9 cut(s) 67, 138, 187, 202, 254, 274, 280, 292, 351
SfaNI GCATC 1 cut(s) 395
SfcI CTRYAG 2 cut(s) 195, 273
SinI GGWCC 1 cut(s) 236
Sse9I AATT 3 cut(s) 258, 311, 324
SsiI CCGC 1 cut(s) 408
StyD4I CCNGG 3 cut(s) 232, 251, 400
StyI CCWWGG 1 cut(s) 422
TaqI TCGA 2 cut(s) 11, 204
TasI AATT 3 cut(s) 258, 311, 324
TfiI GAWTC 2 cut(s) 101, 441
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 2 cut(s) 93, 407
TspGWI ACGGA 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 236
XapI RAATTY 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.