Rroxscaffold_2G00152900

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001174
Physical Location & Seq
Forward (+)
89732681 .. 89733802
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152900.1

Sequence Viewer

Length: 204 bp
ATGTTTTGCATTCCTTTTAGTAATGCCTTTTCTAACATGTTGCTGCAGGAATATAAGGAGAAGCCAGATTATGCACGTTGTGTTGCTATTGTAGCATCTGCATCTTTGAGGGAGATGATAAAACCTGGTGCCTTGGCTATTATTTCACCTATAGCAGTTGGTATTGTGTTCTGGATATTTGGATACTATCCGGAGGCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

67

Amino Acids

7.49

Weight (kDa)

7.75

Isoelectric Point (pI)

25.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 14 - 57 3.4e-11 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AccIII TCCGGA 1 cut(s) 190
AdeI CACNNNGTG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 36
AjnI CCWGG 1 cut(s) 124
Aor13HI TCCGGA 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 138
BanI GGYRCC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 126
BciVI GTATCC 1 cut(s) 176
BfmI CTRYAG 2 cut(s) 44, 150
BfuI GTATCC 1 cut(s) 176
BisI GCNGC 1 cut(s) 44
BlsI GCNGC 1 cut(s) 45
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 2 cut(s) 104, 110
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 1 cut(s) 185
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 191
BsmI GAATGC 1 cut(s) 9
Bsp13I TCCGGA 1 cut(s) 190
BspEI TCCGGA 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 126
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 2 cut(s) 44, 150
BstV1I GCAGC 1 cut(s) 30
BsuI GTATCC 1 cut(s) 176
CsiI ACCWGGT 1 cut(s) 124
CviAII CATG 1 cut(s) 37
CviJI RGCY 2 cut(s) 64, 137
CviKI_1 RGCY 2 cut(s) 64, 137
DraIII CACNNNGTG 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 1 cut(s) 40
FaiI YATR 4 cut(s) 38, 54, 72, 152
FatI CATG 1 cut(s) 36
Fnu4HI GCNGC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 44
GluI GCNGC 1 cut(s) 44
HapII CCGG 1 cut(s) 191
Hin1II CATG 1 cut(s) 40
HpaII CCGG 1 cut(s) 191
HphI GGTGA 1 cut(s) 138
Hpy188III TCNNGA 2 cut(s) 172, 191
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 4 cut(s) 9, 46, 74, 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 190
LpnPI CCDG 5 cut(s) 32, 78, 111, 138, 157
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 2 cut(s) 104, 110
MabI ACCWGGT 1 cut(s) 124
MaeII ACGT 1 cut(s) 76
MnlI CCTC 2 cut(s) 102, 187
MroI TCCGGA 1 cut(s) 190
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 9
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 1 cut(s) 130
NspI RCATGY 1 cut(s) 40
PciI ACATGT 1 cut(s) 36
PctI GAATGC 1 cut(s) 9
PkrI GCNGC 1 cut(s) 45
PscI ACATGT 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 130
PstI CTGCAG 1 cut(s) 48
SatI GCNGC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 126
SetI ASST 3 cut(s) 79, 127, 151
SexAI ACCWGGT 1 cut(s) 124
SfaNI GCATC 2 cut(s) 104, 110
SfcI CTRYAG 2 cut(s) 44, 150
SgeI CNNG 8 cut(s) 49, 59, 77, 87, 137, 138, 145, 184
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 132
TaiI ACGT 1 cut(s) 79
TseI GCWGC 1 cut(s) 43
XceI RCATGY 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.