Rroxscaffold_6G00388010

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001174
Physical Location & Seq
Reverse (-)
502922 .. 506870
3949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00388010.1

Sequence Viewer

Length: 819 bp
ATGGACAAGGACAAGAGCCGTACCGATCTGCTCGCTGCTGGCCGTAAAAAGGAATTGTCAACAGATTCATCCCAGCACGTTCGCTCTGCATTGGCTTCAGTGATAATGGGAATGGCACCAGATGCAACCATAGAGCAACTGCTGCCTATTTTCCTTTCTCTTCTCAAAGATGAGTTTCCAGATGTCCGGCTGAACATTATTAGCAAGGTTGATCAAGTCAACCAGGGAATTGGAATTGATCTGCTGTCCCAGTCTCTACTGCCAGCAATTGTGGAACTGGCAGAGGATAGACATTGGAGGGTTCGGCTTGCGATAATAGAATATATCCCTTTATTGGCAAGTCAGTTGGGTGTGGGGTTTTTCGATGATAAGCTTGGTTCTCTTTGCATGCAGTGGTTAAATGATAAGGTTTACTCAATACGTGATGCAGCTGCTAATAATGTGAAGCGCCTTGCAGAAGAATTTGGTCCTGATTGGGCCATGCAGCATATAGTCCCACAGGTTTTGGAAATGATTAATAACCCTCACTATTTGTATCGGATGACCATTCTACATGCAATTTCCCTACTTGCCCCTGTTATGGGATCAGAAATTACTTGTTCCAAACTTCTACCGGTGGTTATCACTGCATCAAAAGATAGGTATTGCGTTCCGGATATTTGGATACTATCCGGAGGCAAAGGAAGTGATTGTCATAAGGCGGCAATTACAGGAGACATAGTGGGAGACCCATTCAAAGACACAGCTAGACCTTCACTTCATGTCCTTATTAAAATGCTTGCTACAAAAATGTTGGTCATGGCTCCTGTCTTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

272

Amino Acids

30.14

Weight (kDa)

6.24

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP2R1A-like_HEAT PF22646 16 - 40 8.6e-07 PPP2R1A-like HEAT repeat
H_PPase PF03030 225 - 267 2.3e-15 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 115
AccIII TCCGGA 2 cut(s) 652, 671
AciI CCGC 1 cut(s) 701
AclWI GGATC 1 cut(s) 592
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 1 cut(s) 461
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 4 cut(s) 49, 334, 580, 581
AgeI ACCGGT 1 cut(s) 613
AgsI TTSAA 1 cut(s) 736
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 3 cut(s) 373, 431, 746
AluI AGCT 3 cut(s) 373, 431, 746
Alw26I GTCTC 3 cut(s) 258, 708, 720
AlwI GGATC 1 cut(s) 592
Aor13HI TCCGGA 2 cut(s) 652, 671
AoxI GGCC 2 cut(s) 40, 477
ApeKI GCWGC 5 cut(s) 35, 142, 428, 431, 484
ApoI RAATTY 1 cut(s) 461
AseI ATTAAT 1 cut(s) 516
AsiGI ACCGGT 1 cut(s) 613
AspLEI GCGC 1 cut(s) 450
AspS9I GGNCC 2 cut(s) 467, 477
AvaII GGWCC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 115
BbvI GCAGC 5 cut(s) 22, 129, 418, 440, 496
BceAI ACGGC 2 cut(s) 3, 27
BciT130I CCWGG 1 cut(s) 224
BciVI GTATCC 1 cut(s) 657
BclI TGATCA 1 cut(s) 211
BcoDI GTCTC 3 cut(s) 258, 708, 720
BfaI CTAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 451
BfuI GTATCC 1 cut(s) 657
BisI GCNGC 6 cut(s) 36, 143, 429, 432, 485, 702
BlsI GCNGC 6 cut(s) 37, 144, 430, 433, 486, 703
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 467, 477
BmiI GGNNCC 2 cut(s) 117, 804
BmrFI CCNGG 1 cut(s) 224
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 3 cut(s) 112, 415, 638
BmuI ACTGGG 1 cut(s) 244
BsaAI YACGTR 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 720
BsaJI CCNNGG 1 cut(s) 223
BsaWI WCCGGW 3 cut(s) 613, 652, 671
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 334, 580, 581
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 2 cut(s) 250, 282
BseAI TCCGGA 2 cut(s) 652, 671
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 2 cut(s) 68, 546
BseLI CCNNNNNNNGG 4 cut(s) 49, 334, 580, 581
BseNI ACTGG 2 cut(s) 250, 282
BseXI GCAGC 5 cut(s) 22, 129, 418, 440, 496
BseYI CCCAGC 1 cut(s) 72
BshFI GGCC 2 cut(s) 42, 479
BshNI GGYRCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 613
BsiSI CCGG 4 cut(s) 187, 614, 653, 672
BslFI GGGAC 2 cut(s) 232, 479
BslI CCNNNNNNNGG 4 cut(s) 49, 334, 580, 581
BsmAI GTCTC 3 cut(s) 258, 708, 720
BsmFI GGGAC 2 cut(s) 232, 479
BsnI GGCC 2 cut(s) 42, 479
Bso31I GGTCTC 1 cut(s) 720
Bsp13I TCCGGA 2 cut(s) 652, 671
Bsp143I GATC 4 cut(s) 25, 211, 238, 584
BspACI CCGC 1 cut(s) 701
BspANI GGCC 2 cut(s) 42, 479
BspEI TCCGGA 2 cut(s) 652, 671
BspLI GGNNCC 2 cut(s) 117, 804
BspPI GGATC 1 cut(s) 592
BspT107I GGYRCC 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 720
BsrFI RCCGGY 1 cut(s) 613
BsrI ACTGG 2 cut(s) 250, 282
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 4 cut(s) 25, 211, 238, 584
Bst2UI CCWGG 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 165
BstAPI GCANNNNNTGC 2 cut(s) 122, 142
BstBAI YACGTR 1 cut(s) 422
BstC8I GCNNGC 6 cut(s) 33, 40, 264, 309, 389, 780
BstF5I GGATG 2 cut(s) 68, 546
BstH2I RGCGCY 1 cut(s) 451
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 4 cut(s) 28, 214, 241, 587
BstMAI GTCTC 3 cut(s) 258, 708, 720
BstMBI GATC 4 cut(s) 25, 211, 238, 584
BstMWI GCNNNNNNNGC 2 cut(s) 122, 142
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 2 cut(s) 391, 557
BstSCI CCNGG 1 cut(s) 222
BstV1I GCAGC 5 cut(s) 22, 129, 418, 440, 496
BstXI CCANNNNNNTGG 1 cut(s) 230
BsuI GTATCC 1 cut(s) 657
BsuRI GGCC 2 cut(s) 42, 479
BtsCI GGATG 2 cut(s) 68, 546
BtsI GCAGTG 2 cut(s) 398, 624
BtsIMutI CAGTG 3 cut(s) 105, 398, 624
Cac8I GCNNGC 6 cut(s) 33, 40, 264, 309, 389, 780
CfoI GCGC 1 cut(s) 450
Cfr10I RCCGGY 1 cut(s) 613
Cfr13I GGNCC 2 cut(s) 467, 477
Csp6I GTAC 1 cut(s) 21
CspAI ACCGGT 1 cut(s) 613
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CviAII CATG 5 cut(s) 388, 481, 554, 761, 799
CviQI GTAC 1 cut(s) 21
DpnI GATC 4 cut(s) 27, 213, 240, 586
DpnII GATC 4 cut(s) 25, 211, 238, 584
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 720
Eco47I GGWCC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 222
FaeI CATG 5 cut(s) 391, 484, 557, 764, 802
FaqI GGGAC 2 cut(s) 232, 479
FatI CATG 5 cut(s) 387, 480, 553, 760, 798
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 6 cut(s) 36, 143, 429, 432, 485, 702
FokI GGATG 2 cut(s) 55, 553
Fsp4HI GCNGC 6 cut(s) 36, 143, 429, 432, 485, 702
FspBI CTAG 1 cut(s) 747
GlaI GCGC 1 cut(s) 449
GluI GCNGC 6 cut(s) 36, 143, 429, 432, 485, 702
GsaI CCCAGC 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 451
HaeIII GGCC 2 cut(s) 42, 479
HapII CCGG 4 cut(s) 187, 614, 653, 672
HhaI GCGC 1 cut(s) 450
Hin1II CATG 5 cut(s) 391, 484, 557, 764, 802
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HincII GTYRAC 2 cut(s) 60, 220
HindII GTYRAC 2 cut(s) 60, 220
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 1 cut(s) 65
HpaII CCGG 4 cut(s) 187, 614, 653, 672
Hpy166II GTNNAC 3 cut(s) 60, 220, 412
Hpy188I TCNGA 2 cut(s) 540, 589
Hpy188III TCNNGA 4 cut(s) 179, 470, 653, 672
Hpy8I GTNNAC 3 cut(s) 60, 220, 412
HpyAV CCTTC 1 cut(s) 762
HpyCH4IV ACGT 2 cut(s) 78, 421
HpyCH4V TGCA 9 cut(s) 89, 125, 387, 391, 428, 455, 484, 557, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 142
HpySE526I ACGT 2 cut(s) 78, 421
Hsp92II CATG 5 cut(s) 391, 484, 557, 764, 802
HspAI GCGC 1 cut(s) 448
Kpn2I TCCGGA 2 cut(s) 652, 671
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 4 cut(s) 25, 211, 238, 584
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 5 cut(s) 22, 129, 418, 440, 496
LweI GCATC 3 cut(s) 112, 415, 638
MaeI CTAG 1 cut(s) 747
MaeII ACGT 2 cut(s) 78, 421
MalI GATC 4 cut(s) 27, 213, 240, 586
MboI GATC 4 cut(s) 25, 211, 238, 584
MboII GAAGA 2 cut(s) 152, 470
MfeI CAATTG 1 cut(s) 267
MluCI AATT 8 cut(s) 53, 228, 234, 267, 461, 558, 591, 705
MnlI CCTC 4 cut(s) 277, 291, 534, 668
MroI TCCGGA 2 cut(s) 652, 671
MseI TTAA 3 cut(s) 398, 516, 771
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 4 cut(s) 187, 614, 653, 672
MspR9I CCNGG 1 cut(s) 224
MunI CAATTG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 122, 142
NdeII GATC 4 cut(s) 25, 211, 238, 584
NlaIII CATG 5 cut(s) 391, 484, 557, 764, 802
NlaIV GGNNCC 2 cut(s) 117, 804
NspI RCATGY 2 cut(s) 391, 557
PaeI GCATGC 1 cut(s) 391
PfeI GAWTC 1 cut(s) 65
PinAI ACCGGT 1 cut(s) 613
PkrI GCNGC 6 cut(s) 37, 144, 430, 433, 486, 703
Ppu21I YACGTR 1 cut(s) 422
PshBI ATTAAT 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 222
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 2 cut(s) 117, 804
PspPI GGNCC 2 cut(s) 467, 477
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
SaqAI TTAA 3 cut(s) 398, 516, 771
SatI GCNGC 6 cut(s) 36, 143, 429, 432, 485, 702
Sau3AI GATC 4 cut(s) 25, 211, 238, 584
Sau96I GGNCC 2 cut(s) 467, 477
ScrFI CCNGG 1 cut(s) 224
SfaNI GCATC 3 cut(s) 112, 415, 638
SinI GGWCC 1 cut(s) 467
SphI GCATGC 1 cut(s) 391
Sse9I AATT 8 cut(s) 53, 228, 234, 267, 461, 558, 591, 705
SsiI CCGC 1 cut(s) 701
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 222
TaiI ACGT 2 cut(s) 81, 424
TaqI TCGA 1 cut(s) 363
TasI AATT 8 cut(s) 53, 228, 234, 267, 461, 558, 591, 705
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 3 cut(s) 398, 516, 771
Tru9I TTAA 3 cut(s) 398, 516, 771
TscAI CASTG 3 cut(s) 105, 398, 631
TseI GCWGC 5 cut(s) 35, 142, 428, 431, 484
TspDTI ATGAA 3 cut(s) 57, 749, 803
TspRI CASTG 3 cut(s) 105, 398, 631
VpaK11BI GGWCC 1 cut(s) 467
VspI ATTAAT 1 cut(s) 516
XapI RAATTY 1 cut(s) 461
XceI RCATGY 2 cut(s) 391, 557
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.