pycom13g03920

Serine threonine-protein phosphatase 2A 65 kDa regulatory subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001174
Physical Location & Seq
Forward (+)
2591763 .. 2594983
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03920.2

Sequence Viewer

Length: 1125 bp
ATGCAAGATTCTGTTCGACTCTTGGCCGTTGAGGGCTGTGCTGCTCTTGGGAAGTTGTTGGAGCCCCAAGATTGTGTTGCACATATCCTCCCTGTTATTGTTAACTTCTCTCAGGATAAGTCTTGGCGTGTGCGTTACATGGTTGCAAATCAACTATATGAGCTTTGTGAAGCTGTAGGGCCTGAGCCTACAAGGACGGACCTGGTTCCGGCATATGTGCGATTGCTTCGAGATAATGAGGCTGAAGTACGCATAGCAGCTGCTGGCAAAGTTACCAAATTTTGCCGGATTTTAAGTCCAGAACTTGCAATTCAGCATATTCTTCCATGTGTCAAGGAGCTGTCATCAGATTCTTCCCAACATGTCCGGTCTGCTTTGGCTTCAGTTATAATGGGAATGGCTCTTGTATTAGGAAAGGATGCTACAATTGAGCAGCTCCTTCCCATTTTTCTTTCACTCCTGAAGGATGAATTTCCTGATGTGCGTCTTAATATCATTAGCAAGCTTGATCAAGTGAATCAGGTTATTGGAATTGATTTGCTGTCTCAATCCTTGTTACCAGCCATAGTTGAGCTAGCGGAGGATAGACATTGGAGGGTTCGACTGGCAATAATAGAGTACATACCTTTATTGGCAAGTCAGCTGGGTGTAGGGTTCTTTGATGATAAGCTTGGTGCCCTTTGCATGCAGTGGTTACAAGACAAGGTCTACTCAATTCGTGATGCTGCTGCTAATAATTTAAAGCGTCTGGCAGAAGAGTTCGGTCCAGAATGGGCAATGCAACATATTGTTCCACAGGTCCTTCAGATGATAGACAATCCACACTATTTGTATCGAATGACAATTCTGCGTGCAATCTGTCTCCTTGCCCCGGTTATGGGTCCAGAAATCACATGCTCAAAACTGCTGCCAGTGCTCGTCAATGCGACAAAGGACAGAGTACCGAACATCAAATTCAATGTCGCAAAGGTGCTGCAGTCCCTTATCCCCATAGTTGATCAGTCTGTGGTGGAGAAAACAATTCGTCCCTCTTTGGTTGAGCTCAGCGAGGACCCAGATGTTGACGTCCGGTTTTTCGCTAACCAGGCACTTCAGGCAATCGATCATGTCATGATGTCAAGCTAG

Protein Analysis

375

Amino Acids

41.77

Weight (kDa)

5.47

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AatII GACGTC 1 cut(s) 1068
AccB1I GGYRCC 1 cut(s) 674
AccI GTMKAC 1 cut(s) 708
AciI CCGC 1 cut(s) 578
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 3 cut(s) 278, 470, 953
AcuI CTGAAG 5 cut(s) 264, 366, 482, 788, 1076
AcyI GRCGYC 1 cut(s) 1065
AfaI GTAC 3 cut(s) 249, 620, 942
AfiI CCNNNNNNNGG 4 cut(s) 208, 871, 877, 878
AflIII ACRYGT 1 cut(s) 361
AgsI TTSAA 1 cut(s) 958
AjnI CCWGG 2 cut(s) 201, 1083
Alw21I GWGCWC 2 cut(s) 918, 1044
Alw26I GTCTC 2 cut(s) 549, 866
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 2 cut(s) 24, 179
ApeKI GCWGC 8 cut(s) 41, 257, 260, 433, 725, 728, 907, 973
ApoI RAATTY 3 cut(s) 278, 470, 953
ArsI GACNNNNNNTTYG 2 cut(s) 945, 977
AspS9I GGNCC 6 cut(s) 179, 199, 764, 799, 881, 1051
AsuC2I CCSGG 1 cut(s) 872
AsuNHI GCTAGC 1 cut(s) 574
AvaII GGWCC 5 cut(s) 199, 764, 799, 881, 1051
BaeGI GKGCMC 1 cut(s) 679
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 2 cut(s) 66, 1044
Bbv12I GWGCWC 2 cut(s) 918, 1044
BbvI GCAGC 8 cut(s) 28, 247, 269, 445, 712, 715, 894, 960
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 2 cut(s) 203, 1085
BclI TGATCA 2 cut(s) 508, 997
BcnI CCSGG 1 cut(s) 872
BcoDI GTCTC 2 cut(s) 549, 866
BfaI CTAG 2 cut(s) 575, 1123
BfmI CTRYAG 2 cut(s) 174, 974
BisI GCNGC 8 cut(s) 42, 258, 261, 434, 726, 729, 908, 974
BlpI GCTNAGC 1 cut(s) 1043
BlsI GCNGC 8 cut(s) 43, 259, 262, 435, 727, 730, 909, 975
Bme1390I CCNGG 3 cut(s) 203, 872, 1085
Bme18I GGWCC 5 cut(s) 199, 764, 799, 881, 1051
BmgT120I GGNCC 6 cut(s) 179, 199, 764, 799, 881, 1051
BmiI GGNNCC 5 cut(s) 63, 207, 676, 882, 1053
BmrFI CCNGG 3 cut(s) 203, 872, 1085
BmsI GCATC 2 cut(s) 409, 712
BmtI GCTAGC 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 183
Bpu1102I GCTNAGC 1 cut(s) 1043
BpuMI CCSGG 1 cut(s) 872
Bsa29I ATCGAT 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 1065
BsaJI CCNNGG 1 cut(s) 870
BsaWI WCCGGW 2 cut(s) 366, 1068
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 871, 877, 878
Bse1I ACTGG 2 cut(s) 609, 911
Bse3DI GCAATG 1 cut(s) 783
BseBI CCWGG 2 cut(s) 203, 1085
BseCI ATCGAT 1 cut(s) 1101
BseDI CCNNGG 1 cut(s) 870
BseGI GGATG 2 cut(s) 424, 472
BseLI CCNNNNNNNGG 4 cut(s) 208, 871, 877, 878
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 3 cut(s) 125, 174, 1057
BseNI ACTGG 2 cut(s) 609, 911
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 8 cut(s) 28, 247, 269, 445, 712, 715, 894, 960
BseYI CCCAGC 1 cut(s) 643
BshFI GGCC 2 cut(s) 26, 181
BshNI GGYRCC 1 cut(s) 674
BshVI ATCGAT 1 cut(s) 1101
BsiHKAI GWGCWC 2 cut(s) 918, 1044
BsiSI CCGG 5 cut(s) 209, 286, 367, 872, 1069
BslFI GGGAC 2 cut(s) 964, 1011
BslI CCNNNNNNNGG 4 cut(s) 208, 871, 877, 878
BsmAI GTCTC 2 cut(s) 549, 866
BsmFI GGGAC 2 cut(s) 964, 1011
BsnI GGCC 2 cut(s) 26, 181
Bsp1286I GDGCHC 4 cut(s) 66, 679, 918, 1044
Bsp143I GATC 3 cut(s) 508, 997, 1102
Bsp1720I GCTNAGC 1 cut(s) 1043
BspACI CCGC 1 cut(s) 578
BspANI GGCC 2 cut(s) 26, 181
BspCNI CTCAG 3 cut(s) 124, 175, 1056
BspDI ATCGAT 1 cut(s) 1101
BspHI TCATGA 1 cut(s) 1110
BspLI GGNNCC 5 cut(s) 63, 207, 676, 882, 1053
BspMAI CTGCAG 1 cut(s) 978
BspOI GCTAGC 1 cut(s) 578
BspT107I GGYRCC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 2 cut(s) 609, 911
BssECI CCNNGG 1 cut(s) 870
BssMI GATC 3 cut(s) 508, 997, 1102
BssNI GRCGYC 1 cut(s) 1065
Bst2UI CCWGG 2 cut(s) 203, 1085
Bst6I CTCTTC 1 cut(s) 750
BstACI GRCGYC 1 cut(s) 1065
BstC8I GCNNGC 5 cut(s) 265, 503, 576, 686, 852
BstDEI CTNAG 3 cut(s) 111, 183, 1043
BstF5I GGATG 2 cut(s) 424, 472
BstKTI GATC 3 cut(s) 511, 1000, 1105
BstMAI GTCTC 2 cut(s) 549, 866
BstMBI GATC 3 cut(s) 508, 997, 1102
BstMWI GCNNNNNNNGC 3 cut(s) 913, 1085, 1094
BstNI CCWGG 2 cut(s) 203, 1085
BstNSI RCATGY 3 cut(s) 365, 688, 897
BstSCI CCNGG 3 cut(s) 201, 870, 1083
BstSFI CTRYAG 2 cut(s) 174, 974
BstSLI GKGCMC 1 cut(s) 679
BstV1I GCAGC 8 cut(s) 28, 247, 269, 445, 712, 715, 894, 960
Bsu15I ATCGAT 1 cut(s) 1101
BsuRI GGCC 2 cut(s) 26, 181
BsuTUI ATCGAT 1 cut(s) 1101
BtsCI GGATG 2 cut(s) 424, 472
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 2 cut(s) 695, 918
Cac8I GCNNGC 5 cut(s) 265, 503, 576, 686, 852
CaiI CAGNNNCTG 1 cut(s) 263
CciI TCATGA 1 cut(s) 1110
Cfr13I GGNCC 6 cut(s) 179, 199, 764, 799, 881, 1051
ClaI ATCGAT 1 cut(s) 1101
CseI GACGC 2 cut(s) 473, 734
CsiI ACCWGGT 1 cut(s) 201
Csp6I GTAC 3 cut(s) 248, 619, 941
CspCI CAANNNNNGTGG 2 cut(s) 810, 845
CviAII CATG 7 cut(s) 139, 327, 362, 685, 894, 1106, 1111
CviQI GTAC 3 cut(s) 248, 619, 941
DdeI CTNAG 3 cut(s) 111, 183, 1043
DpnI GATC 3 cut(s) 510, 999, 1104
DpnII GATC 3 cut(s) 508, 997, 1102
DraI TTTAAA 1 cut(s) 741
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 750
EarI CTCTTC 1 cut(s) 750
Ecl136II GAGCTC 1 cut(s) 1042
Eco24I GRGCYC 2 cut(s) 66, 1044
Eco47I GGWCC 5 cut(s) 199, 764, 799, 881, 1051
Eco53kI GAGCTC 1 cut(s) 1042
Eco57I CTGAAG 5 cut(s) 264, 366, 482, 788, 1076
EcoICRI GAGCTC 1 cut(s) 1042
EcoO109I RGGNCCY 3 cut(s) 179, 799, 1051
EcoRII CCWGG 2 cut(s) 201, 1083
EcoT38I GRGCYC 2 cut(s) 66, 1044
FaeI CATG 7 cut(s) 142, 330, 365, 688, 897, 1109, 1114
FaqI GGGAC 2 cut(s) 964, 1011
FatI CATG 7 cut(s) 138, 326, 361, 684, 893, 1105, 1110
FauNDI CATATG 1 cut(s) 214
FbaI TGATCA 2 cut(s) 508, 997
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 8 cut(s) 42, 258, 261, 434, 726, 729, 908, 974
FokI GGATG 2 cut(s) 431, 479
FriOI GRGCYC 2 cut(s) 66, 1044
Fsp4HI GCNGC 8 cut(s) 42, 258, 261, 434, 726, 729, 908, 974
FspBI CTAG 2 cut(s) 575, 1123
GluI GCNGC 8 cut(s) 42, 258, 261, 434, 726, 729, 908, 974
GsaI CCCAGC 1 cut(s) 647
HaeIII GGCC 2 cut(s) 26, 181
HapII CCGG 5 cut(s) 209, 286, 367, 872, 1069
HgaI GACGC 2 cut(s) 473, 734
Hin1I GRCGYC 1 cut(s) 1065
Hin1II CATG 7 cut(s) 142, 330, 365, 688, 897, 1109, 1114
HincII GTYRAC 2 cut(s) 103, 1063
HindII GTYRAC 2 cut(s) 103, 1063
HindIII AAGCTT 2 cut(s) 503, 668
HinfI GANTC 4 cut(s) 8, 18, 350, 517
HpaI GTTAAC 1 cut(s) 103
HpaII CCGG 5 cut(s) 209, 286, 367, 872, 1069
Hpy166II GTNNAC 3 cut(s) 103, 709, 1063
Hpy188I TCNGA 2 cut(s) 349, 807
Hpy188III TCNNGA 9 cut(s) 113, 230, 299, 460, 476, 719, 767, 884, 1111
Hpy8I GTNNAC 3 cut(s) 103, 709, 1063
HpyAV CCTTC 3 cut(s) 449, 457, 812
HpyCH4IV ACGT 1 cut(s) 1065
HpyCH4V TGCA 9 cut(s) 4, 80, 146, 308, 684, 688, 781, 854, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 913, 1085, 1094
HpyF3I CTNAG 3 cut(s) 111, 183, 1043
HpySE526I ACGT 1 cut(s) 1065
Hsp92I GRCGYC 1 cut(s) 1065
Hsp92II CATG 7 cut(s) 142, 330, 365, 688, 897, 1109, 1114
Ksp22I TGATCA 2 cut(s) 508, 997
KspAI GTTAAC 1 cut(s) 103
Kzo9I GATC 3 cut(s) 508, 997, 1102
LmnI GCTCC 3 cut(s) 61, 337, 441
Lsp1109I GCAGC 8 cut(s) 28, 247, 269, 445, 712, 715, 894, 960
LweI GCATC 2 cut(s) 409, 712
MabI ACCWGGT 1 cut(s) 201
MaeI CTAG 2 cut(s) 575, 1123
MaeII ACGT 1 cut(s) 1065
MaeIII GTNAC 4 cut(s) 134, 271, 555, 693
MalI GATC 3 cut(s) 510, 999, 1104
MboI GATC 3 cut(s) 508, 997, 1102
MboII GAAGA 3 cut(s) 314, 345, 767
MfeI CAATTG 1 cut(s) 426
MhlI GDGCHC 4 cut(s) 66, 679, 918, 1044
MlyI GAGTC 1 cut(s) 12
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 7 cut(s) 25, 98, 232, 574, 588, 1039, 1042
MseI TTAA 4 cut(s) 102, 293, 489, 740
MspA1I CMGCKG 2 cut(s) 260, 643
MspI CCGG 5 cut(s) 209, 286, 367, 872, 1069
MspR9I CCNGG 3 cut(s) 203, 872, 1085
MunI CAATTG 1 cut(s) 426
MvaI CCWGG 2 cut(s) 203, 1085
MwoI GCNNNNNNNGC 3 cut(s) 913, 1085, 1094
NciI CCSGG 1 cut(s) 872
NdeI CATATG 1 cut(s) 214
NdeII GATC 3 cut(s) 508, 997, 1102
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 7 cut(s) 142, 330, 365, 688, 897, 1109, 1114
NlaIV GGNNCC 5 cut(s) 63, 207, 676, 882, 1053
NspI RCATGY 3 cut(s) 365, 688, 897
PaeI GCATGC 1 cut(s) 688
PagI TCATGA 1 cut(s) 1110
PciI ACATGT 1 cut(s) 361
PfeI GAWTC 3 cut(s) 8, 350, 517
PflFI GACNNNGTC 1 cut(s) 704
PkrI GCNGC 8 cut(s) 43, 259, 262, 435, 727, 730, 909, 975
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PpuMI RGGWCCY 2 cut(s) 799, 1051
PscI ACATGT 1 cut(s) 361
PsiI TTATAA 1 cut(s) 389
Psp124BI GAGCTC 1 cut(s) 1044
Psp5II RGGWCCY 2 cut(s) 799, 1051
Psp6I CCWGG 2 cut(s) 201, 1083
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 2 cut(s) 201, 1083
PspN4I GGNNCC 5 cut(s) 63, 207, 676, 882, 1053
PspPI GGNCC 6 cut(s) 179, 199, 764, 799, 881, 1051
PspPPI RGGWCCY 2 cut(s) 799, 1051
PstI CTGCAG 1 cut(s) 978
PstNI CAGNNNCTG 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 704
PvuII CAGCTG 2 cut(s) 260, 643
RsaI GTAC 3 cut(s) 249, 620, 942
RsaNI GTAC 3 cut(s) 248, 619, 941
SacI GAGCTC 1 cut(s) 1044
SaqAI TTAA 4 cut(s) 102, 293, 489, 740
SatI GCNGC 8 cut(s) 42, 258, 261, 434, 726, 729, 908, 974
Sau3AI GATC 3 cut(s) 508, 997, 1102
Sau96I GGNCC 6 cut(s) 179, 199, 764, 799, 881, 1051
SchI GAGTC 1 cut(s) 12
ScrFI CCNGG 3 cut(s) 203, 872, 1085
SduI GDGCHC 4 cut(s) 66, 679, 918, 1044
SexAI ACCWGGT 1 cut(s) 201
SfaNI GCATC 2 cut(s) 409, 712
SfcI CTRYAG 2 cut(s) 174, 974
SinI GGWCC 5 cut(s) 199, 764, 799, 881, 1051
SphI GCATGC 1 cut(s) 688
SsiI CCGC 1 cut(s) 578
SspMI CTAG 2 cut(s) 575, 1123
SstI GAGCTC 1 cut(s) 1044
StyD4I CCNGG 3 cut(s) 201, 870, 1083
TaiI ACGT 1 cut(s) 1068
TaqI TCGA 5 cut(s) 16, 229, 601, 835, 1101
TaqII GACCGA 1 cut(s) 752
TatI WGTACW 1 cut(s) 618
TfiI GAWTC 3 cut(s) 8, 350, 517
Tru1I TTAA 4 cut(s) 102, 293, 489, 740
Tru9I TTAA 4 cut(s) 102, 293, 489, 740
TscAI CASTG 2 cut(s) 695, 918
TseI GCWGC 8 cut(s) 41, 257, 260, 433, 725, 728, 907, 973
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 2 cut(s) 695, 918
Tth111I GACNNNGTC 1 cut(s) 704
VpaK11BI GGWCC 5 cut(s) 199, 764, 799, 881, 1051
XapI RAATTY 3 cut(s) 278, 470, 953
XceI RCATGY 3 cut(s) 365, 688, 897
XmiI GTMKAC 1 cut(s) 708
XspI CTAG 2 cut(s) 575, 1123
ZraI GACGTC 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.