Rroxscaffold_6G00404080

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001174
Physical Location & Seq
Reverse (-)
26522957 .. 26525596
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404080.1

Sequence Viewer

Length: 663 bp
ATGGACAAGGACAAGAGCCGTACCGATCTGCTCGCCGCTGGCCGTAAAAAGGGTTTGTTGATGTATAAAAGCATTAGATTGATTGAATTTGACGGTAGTGCTCTTGGTAGTTGTCATGGTTCTATGACTTCTATATATTATCTTGTGCTTCTTCCAAAGAAAGACAAGTCTATTGTTTTGAATATAAGGAGAAGCGATTATGCACGTTGTGTTGCTATTGTAGCATCCGCATCTTTGAGGGAGATGATAAAACCTGGTGCCTTGGCTATTATTTCACCTATAGCAGTTGGTGGGTATTGCGTTCCGGATATTTGGATACTATCCGGAGGCAAAGTAAGTGATTGTCATAATGCTGCAATTACAGGAGACATTGACATTTGGGGAGTGATGGGGAGAGAGTGGGAGAGAATCCGAGAGAGAAAACTCGGGAGTCTTTTGCTTAGCTTTCGAGTTGTTCTTCACACTTGGCCAGTGTCAGCTACGTCGGCGGCAAAACCCGGCTTATCCTCATGGACCGGTCCGTCACCTTCGAAACCCTCCGTCGAGTGCGACGACGACGTGGCGCCGATGATCGCCGAGTTCGAGGCCTCGCAGAGGATTCCCGGTTTACTTGTTAGATCGCACCGCCGGTTCGGCTTGCAGAGAGAATGCGGTTCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

220

Amino Acids

23.97

Weight (kDa)

9.3

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 54 - 98 2.9e-07 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 520
AccB1I GGYRCC 2 cut(s) 257, 562
AccIII TCCGGA 2 cut(s) 304, 323
AciI CCGC 5 cut(s) 36, 228, 488, 625, 651
AcoI YGGCCR 2 cut(s) 40, 467
AcsI RAATTY 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 563
AdeI CACNNNGTG 1 cut(s) 209
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 2 cut(s) 49, 594
AgeI ACCGGT 1 cut(s) 515
AgsI TTSAA 2 cut(s) 86, 181
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 2 cut(s) 444, 479
AluI AGCT 2 cut(s) 444, 479
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 425
Aor13HI TCCGGA 2 cut(s) 304, 323
AoxI GGCC 3 cut(s) 40, 467, 585
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 86
AsiGI ACCGGT 1 cut(s) 515
AspLEI GCGC 1 cut(s) 565
AspS9I GGNCC 2 cut(s) 513, 518
AsuC2I CCSGG 2 cut(s) 498, 603
AsuHPI GGTGA 2 cut(s) 267, 516
AsuII TTCGAA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 2 cut(s) 513, 518
BalI TGGCCA 1 cut(s) 469
BanI GGYRCC 2 cut(s) 257, 562
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 340
BccI CCATC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 3, 27
BciT130I CCWGG 1 cut(s) 255
BciVI GTATCC 1 cut(s) 309
BcnI CCSGG 2 cut(s) 498, 603
BcoDI GTCTC 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 566
BfuI GTATCC 1 cut(s) 309
BglI GCCNNNNNGGC 1 cut(s) 633
BisI GCNGC 3 cut(s) 36, 354, 489
BlpI GCTNAGC 1 cut(s) 440
BlsI GCNGC 3 cut(s) 37, 355, 490
Bme1390I CCNGG 3 cut(s) 255, 498, 603
Bme18I GGWCC 2 cut(s) 513, 518
BmeT110I CYCGRG 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 2 cut(s) 513, 518
BmiI GGNNCC 2 cut(s) 259, 564
BmrFI CCNGG 3 cut(s) 255, 498, 603
BmsI GCATC 2 cut(s) 233, 239
Bpu1102I GCTNAGC 1 cut(s) 440
Bpu14I TTCGAA 1 cut(s) 530
BpuMI CCSGG 2 cut(s) 498, 603
BsaHI GRCGYC 1 cut(s) 563
BsaJI CCNNGG 1 cut(s) 261
BsaWI WCCGGW 3 cut(s) 304, 323, 515
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 594
Bse118I RCCGGY 2 cut(s) 515, 627
Bse1I ACTGG 1 cut(s) 470
BseAI TCCGGA 2 cut(s) 304, 323
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 49, 594
BseNI ACTGG 1 cut(s) 470
BseXI GCAGC 1 cut(s) 340
BshFI GGCC 3 cut(s) 42, 469, 587
BshNI GGYRCC 2 cut(s) 257, 562
BshTI ACCGGT 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 6 cut(s) 305, 324, 498, 516, 603, 628
BslI CCNNNNNNNGG 2 cut(s) 49, 594
BsmAI GTCTC 1 cut(s) 360
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 3 cut(s) 42, 469, 587
BsoBI CYCGRG 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 103
Bsp13I TCCGGA 2 cut(s) 304, 323
Bsp143I GATC 3 cut(s) 25, 570, 617
Bsp1720I GCTNAGC 1 cut(s) 440
BspACI CCGC 5 cut(s) 36, 228, 488, 625, 651
BspANI GGCC 3 cut(s) 42, 469, 587
BspEI TCCGGA 2 cut(s) 304, 323
BspLI GGNNCC 2 cut(s) 259, 564
BspT104I TTCGAA 1 cut(s) 530
BspT107I GGYRCC 2 cut(s) 257, 562
BsrFI RCCGGY 2 cut(s) 515, 627
BsrI ACTGG 1 cut(s) 470
BssAI RCCGGY 2 cut(s) 515, 627
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 3 cut(s) 25, 570, 617
BssNI GRCGYC 1 cut(s) 563
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 95
BstACI GRCGYC 1 cut(s) 563
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 3 cut(s) 33, 40, 638
BstDEI CTNAG 1 cut(s) 440
BstENI CCTNNNNNAGG 1 cut(s) 592
BstF5I GGATG 1 cut(s) 224
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 1 cut(s) 565
BstKTI GATC 3 cut(s) 28, 573, 620
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 3 cut(s) 25, 570, 617
BstMWI GCNNNNNNNGC 3 cut(s) 221, 485, 633
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 3 cut(s) 253, 496, 601
BstSFI CTRYAG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 340
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 3 cut(s) 42, 469, 587
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 477
Cac8I GCNNGC 3 cut(s) 33, 40, 638
CfoI GCGC 1 cut(s) 565
Cfr10I RCCGGY 2 cut(s) 515, 627
Cfr13I GGNCC 2 cut(s) 513, 518
CpoI CGGWCCG 1 cut(s) 518
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 1 cut(s) 21
CspAI ACCGGT 1 cut(s) 515
CspI CGGWCCG 1 cut(s) 518
CviAII CATG 2 cut(s) 116, 510
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 1 cut(s) 440
DinI GGCGCC 1 cut(s) 564
DpnI GATC 3 cut(s) 27, 572, 619
DpnII GATC 3 cut(s) 25, 570, 617
DraIII CACNNNGTG 1 cut(s) 209
DrdI GACNNNNNNGTC 1 cut(s) 520
DseDI GACNNNNNNGTC 1 cut(s) 520
EaeI YGGCCR 2 cut(s) 40, 467
Eco130I CCWWGG 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 587
Eco47I GGWCC 2 cut(s) 513, 518
Eco88I CYCGRG 1 cut(s) 425
EcoNI CCTNNNNNAGG 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 261
EgeI GGCGCC 1 cut(s) 564
EheI GGCGCC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 2 cut(s) 119, 513
FatI CATG 2 cut(s) 115, 509
Fnu4HI GCNGC 3 cut(s) 36, 354, 489
FokI GGATG 1 cut(s) 211
Fsp4HI GCNGC 3 cut(s) 36, 354, 489
GlaI GCGC 1 cut(s) 564
GluI GCNGC 3 cut(s) 36, 354, 489
HaeII RGCGCY 1 cut(s) 566
HaeIII GGCC 3 cut(s) 42, 469, 587
HapII CCGG 6 cut(s) 305, 324, 498, 516, 603, 628
HhaI GCGC 1 cut(s) 565
Hin1I GRCGYC 1 cut(s) 563
Hin1II CATG 2 cut(s) 119, 513
Hin6I GCGC 1 cut(s) 563
HinP1I GCGC 1 cut(s) 563
HinfI GANTC 3 cut(s) 408, 430, 598
HpaII CCGG 6 cut(s) 305, 324, 498, 516, 603, 628
HphI GGTGA 2 cut(s) 267, 516
Hpy166II GTNNAC 1 cut(s) 608
Hpy188I TCNGA 1 cut(s) 413
Hpy188III TCNNGA 3 cut(s) 305, 324, 427
Hpy8I GTNNAC 1 cut(s) 608
Hpy99I CGWCG 5 cut(s) 487, 545, 554, 557, 560
HpyAV CCTTC 1 cut(s) 537
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4IV ACGT 3 cut(s) 205, 482, 558
HpyCH4V TGCA 3 cut(s) 203, 356, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 485, 633
HpyF3I CTNAG 1 cut(s) 440
HpySE526I ACGT 3 cut(s) 205, 482, 558
Hsp92I GRCGYC 1 cut(s) 563
Hsp92II CATG 2 cut(s) 119, 513
HspAI GCGC 1 cut(s) 563
KasI GGCGCC 1 cut(s) 562
Kpn2I TCCGGA 2 cut(s) 304, 323
Kzo9I GATC 3 cut(s) 25, 570, 617
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 2 cut(s) 233, 239
MabI ACCWGGT 1 cut(s) 253
MaeII ACGT 3 cut(s) 205, 482, 558
MaeIII GTNAC 1 cut(s) 522
MalI GATC 3 cut(s) 27, 572, 619
MboI GATC 3 cut(s) 25, 570, 617
MboII GAAGA 2 cut(s) 143, 449
MhlI GDGCHC 1 cut(s) 103
MlsI TGGCCA 1 cut(s) 469
MluCI AATT 2 cut(s) 86, 357
MluNI TGGCCA 1 cut(s) 469
Mly113I GGCGCC 1 cut(s) 563
MlyI GAGTC 1 cut(s) 439
MnlI CCTC 7 cut(s) 231, 320, 517, 547, 577, 588, 598
Mox20I TGGCCA 1 cut(s) 469
MroI TCCGGA 2 cut(s) 304, 323
MscI TGGCCA 1 cut(s) 469
Msp20I TGGCCA 1 cut(s) 469
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 6 cut(s) 305, 324, 498, 516, 603, 628
MspR9I CCNGG 3 cut(s) 255, 498, 603
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 221, 485, 633
NarI GGCGCC 1 cut(s) 563
NciI CCSGG 2 cut(s) 498, 603
NdeII GATC 3 cut(s) 25, 570, 617
NlaIII CATG 2 cut(s) 119, 513
NlaIV GGNNCC 2 cut(s) 259, 564
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 1 cut(s) 522
NspV TTCGAA 1 cut(s) 530
PceI AGGCCT 1 cut(s) 587
PcsI WCGNNNNNNNCGW 2 cut(s) 549, 579
PctI GAATGC 1 cut(s) 653
PfeI GAWTC 2 cut(s) 408, 598
PinAI ACCGGT 1 cut(s) 515
PkrI GCNGC 3 cut(s) 37, 355, 490
PleI GAGTC 1 cut(s) 438
PluTI GGCGCC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 259, 564
PspPI GGNCC 2 cut(s) 513, 518
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
Rsr2I CGGWCCG 1 cut(s) 518
RsrII CGGWCCG 1 cut(s) 518
SatI GCNGC 3 cut(s) 36, 354, 489
Sau3AI GATC 3 cut(s) 25, 570, 617
Sau96I GGNCC 2 cut(s) 513, 518
SchI GAGTC 1 cut(s) 439
ScrFI CCNGG 3 cut(s) 255, 498, 603
SduI GDGCHC 1 cut(s) 103
SetI ASST 8 cut(s) 208, 256, 280, 446, 481, 485, 529, 561
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 2 cut(s) 233, 239
SfcI CTRYAG 1 cut(s) 279
SfoI GGCGCC 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 2 cut(s) 513, 518
Sse9I AATT 2 cut(s) 86, 357
SseBI AGGCCT 1 cut(s) 587
SsiI CCGC 5 cut(s) 36, 228, 488, 625, 651
SspDI GGCGCC 1 cut(s) 562
StuI AGGCCT 1 cut(s) 587
StyD4I CCNGG 3 cut(s) 253, 496, 601
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 95
TaiI ACGT 3 cut(s) 208, 485, 561
TaqI TCGA 4 cut(s) 448, 530, 543, 582
TasI AATT 2 cut(s) 86, 357
TauI GCSGC 2 cut(s) 38, 491
TfiI GAWTC 2 cut(s) 408, 598
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 1 cut(s) 522
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 522
TspGWI ACGGA 2 cut(s) 510, 529
TspRI CASTG 1 cut(s) 477
VpaK11BI GGWCC 2 cut(s) 513, 518
XagI CCTNNNNNAGG 1 cut(s) 592
XapI RAATTY 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.