pyrophosphate-energized membrane proton pump
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| ABC_membrane | PF00664 | 39 - 310 | 4.1e-59 | ABC transporter transmembrane region |
| ABC_tran | PF00005 | 381 - 526 | 1.2e-31 | ABC transporter |
| ABC_membrane | PF00664 | 698 - 970 | 5.5e-60 | ABC transporter transmembrane region |
| ABC_tran | PF00005 | 1039 - 1188 | 3.7e-33 | ABC transporter |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2285 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 955 |
| Acc16I | TGCGCA | 1 cut(s) | 3645 |
| Acc36I | ACCTGC | 2 cut(s) | 289, 2285 |
| AccB1I | GGYRCC | 1 cut(s) | 2390 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1250 |
| AccBSI | CCGCTC | 1 cut(s) | 1164 |
| AccI | GTMKAC | 1 cut(s) | 3185 |
| AccII | CGCG | 1 cut(s) | 633 |
| AciI | CCGC | 5 cut(s) | 513, 633, 1164, 2376, 3649 |
| AclWI | GGATC | 6 cut(s) | 865, 1028, 1235, 1748, 2018, 2366 |
| AcoI | YGGCCR | 2 cut(s) | 1921, 2061 |
| AcsI | RAATTY | 8 cut(s) | 342, 1551, 2275, 2316, 2380, 2585, 2864, 3537 |
| AcuI | CTGAAG | 2 cut(s) | 2004, 3396 |
| AdeI | CACNNNGTG | 1 cut(s) | 2192 |
| AfeI | AGCGCT | 1 cut(s) | 1579 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 1250, 2055, 2144, 2254, 3227, 3459, 3513 |
| AflIII | ACRYGT | 2 cut(s) | 3070, 3367 |
| AgeI | ACCGGT | 1 cut(s) | 1823 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 663, 695 |
| Alw21I | GWGCWC | 2 cut(s) | 2349, 3568 |
| Alw26I | GTCTC | 5 cut(s) | 252, 307, 963, 1001, 3111 |
| Alw44I | GTGCAC | 2 cut(s) | 2345, 3564 |
| AlwI | GGATC | 6 cut(s) | 865, 1028, 1235, 1748, 2018, 2366 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 656, 748, 1250 |
| Ama87I | CYCGRG | 1 cut(s) | 3013 |
| Aor51HI | AGCGCT | 1 cut(s) | 1579 |
| AoxI | GGCC | 6 cut(s) | 280, 892, 1118, 1921, 2061, 3196 |
| ApaLI | GTGCAC | 2 cut(s) | 2345, 3564 |
| ApoI | RAATTY | 8 cut(s) | 342, 1551, 2275, 2316, 2380, 2585, 2864, 3537 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3139, 3171 |
| AseI | ATTAAT | 2 cut(s) | 1628, 2819 |
| AsiGI | ACCGGT | 1 cut(s) | 1823 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 2279, 3315, 3333 |
| AspLEI | GCGC | 2 cut(s) | 1580, 3646 |
| AspS9I | GGNCC | 4 cut(s) | 892, 2732, 3339, 3529 |
| AsuHPI | GGTGA | 7 cut(s) | 328, 541, 945, 1265, 2456, 2713, 3631 |
| AsuII | TTCGAA | 1 cut(s) | 2668 |
| AsuNHI | GCTAGC | 1 cut(s) | 2633 |
| AvaI | CYCGRG | 1 cut(s) | 3013 |
| AvaII | GGWCC | 3 cut(s) | 2732, 3339, 3529 |
| AxyI | CCTNAGG | 1 cut(s) | 1458 |
| BaeGI | GKGCMC | 2 cut(s) | 2349, 3568 |
| BalI | TGGCCA | 1 cut(s) | 2063 |
| BanI | GGYRCC | 1 cut(s) | 2390 |
| BanII | GRGCYC | 1 cut(s) | 1417 |
| BbsI | GAAGAC | 3 cut(s) | 169, 2211, 2403 |
| Bbv12I | GWGCWC | 2 cut(s) | 2349, 3568 |
| BbvCI | CCTCAGC | 1 cut(s) | 3281 |
| BceAI | ACGGC | 2 cut(s) | 2464, 3712 |
| BciVI | GTATCC | 1 cut(s) | 1119 |
| BclI | TGATCA | 3 cut(s) | 46, 117, 867 |
| BcoDI | GTCTC | 5 cut(s) | 252, 307, 963, 1001, 3111 |
| BfmI | CTRYAG | 4 cut(s) | 663, 1647, 1787, 2112 |
| BfoI | RGCGCY | 1 cut(s) | 1581 |
| BfuAI | ACCTGC | 2 cut(s) | 289, 2285 |
| BfuI | GTATCC | 1 cut(s) | 1119 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1462 |
| BglII | AGATCT | 1 cut(s) | 1131 |
| BlpI | GCTNAGC | 2 cut(s) | 1862, 2452 |
| BmcAI | AGTACT | 1 cut(s) | 2574 |
| Bme18I | GGWCC | 3 cut(s) | 2732, 3339, 3529 |
| BmeT110I | CYCGRG | 1 cut(s) | 3013 |
| BmgT120I | GGNCC | 4 cut(s) | 892, 2732, 3339, 3529 |
| BmiI | GGNNCC | 8 cut(s) | 685, 893, 1874, 2104, 2392, 2734, 2940, 3531 |
| BmrI | ACTGGG | 1 cut(s) | 330 |
| BmtI | GCTAGC | 1 cut(s) | 2637 |
| BmuI | ACTGGG | 1 cut(s) | 330 |
| BoxI | GACNNNNGTC | 2 cut(s) | 173, 3228 |
| BpiI | GAAGAC | 3 cut(s) | 169, 2211, 2403 |
| BpmI | CTGGAG | 4 cut(s) | 612, 1071, 2244, 3504 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2613, 3281 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1862, 2452 |
| Bpu14I | TTCGAA | 1 cut(s) | 2668 |
| BpuEI | CTTGAG | 3 cut(s) | 225, 384, 1025 |
| BsaBI | GATNNNNATC | 1 cut(s) | 119 |
| BsaI | GGTCTC | 2 cut(s) | 307, 1001 |
| BsaJI | CCNNGG | 3 cut(s) | 462, 552, 2248 |
| BsaWI | WCCGGW | 4 cut(s) | 410, 710, 1823, 2266 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2217, 2247 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 1250, 2055, 2144, 2254, 3227, 3459, 3513 |
| Bse118I | RCCGGY | 2 cut(s) | 1823, 1923 |
| Bse1I | ACTGG | 5 cut(s) | 325, 1054, 2522, 3281, 3573 |
| Bse21I | CCTNAGG | 1 cut(s) | 1458 |
| Bse3DI | GCAATG | 5 cut(s) | 924, 2106, 3387, 3431, 3507 |
| Bse8I | GATNNNNATC | 1 cut(s) | 119 |
| BseDI | CCNNGG | 3 cut(s) | 462, 552, 2248 |
| BseGI | GGATG | 6 cut(s) | 318, 545, 2244, 2713, 3106, 3577 |
| BseJI | GATNNNNATC | 1 cut(s) | 119 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 1250, 2055, 2144, 2254, 3227, 3459, 3513 |
| BseMI | GCAATG | 5 cut(s) | 924, 2106, 3387, 3431, 3507 |
| BseMII | CTCAG | 8 cut(s) | 1309, 1581, 2919, 2979, 3240, 3272, 3468, 3699 |
| BseNI | ACTGG | 5 cut(s) | 325, 1054, 2522, 3281, 3573 |
| BseRI | GAGGAG | 4 cut(s) | 24, 27, 609, 1415 |
| BseSI | GKGCMC | 2 cut(s) | 2349, 3568 |
| BseYI | CCCAGC | 1 cut(s) | 214 |
| BsgI | GTGCAG | 4 cut(s) | 1148, 2291, 2791, 3125 |
| Bsh1236I | CGCG | 1 cut(s) | 633 |
| BshFI | GGCC | 6 cut(s) | 282, 894, 1120, 1923, 2063, 3198 |
| BshNI | GGYRCC | 1 cut(s) | 2390 |
| BshTI | ACCGGT | 1 cut(s) | 1823 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2349, 3568 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3013 |
| BsiSI | CCGG | 5 cut(s) | 411, 711, 1824, 1924, 2267 |
| BslFI | GGGAC | 3 cut(s) | 212, 452, 2718 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 1250, 2055, 2144, 2254, 3227, 3459, 3513 |
| BsmAI | GTCTC | 5 cut(s) | 252, 307, 963, 1001, 3111 |
| BsmBI | CGTCTC | 2 cut(s) | 963, 3111 |
| BsmFI | GGGAC | 3 cut(s) | 212, 452, 2718 |
| BsmI | GAATGC | 1 cut(s) | 2664 |
| BsnI | GGCC | 6 cut(s) | 282, 894, 1120, 1923, 2063, 3198 |
| Bso31I | GGTCTC | 2 cut(s) | 307, 1001 |
| BsoBI | CYCGRG | 1 cut(s) | 3013 |
| Bsp119I | TTCGAA | 1 cut(s) | 2668 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1417, 2349, 3568 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1862, 2452 |
| Bsp19I | CCATGG | 1 cut(s) | 462 |
| BspACI | CCGC | 5 cut(s) | 513, 633, 1164, 2376, 3649 |
| BspANI | GGCC | 6 cut(s) | 282, 894, 1120, 1923, 2063, 3198 |
| BspCNI | CTCAG | 8 cut(s) | 1308, 1582, 2918, 2978, 3239, 3273, 3467, 3700 |
| BspFNI | CGCG | 1 cut(s) | 633 |
| BspHI | TCATGA | 1 cut(s) | 165 |
| BspLI | GGNNCC | 8 cut(s) | 685, 893, 1874, 2104, 2392, 2734, 2940, 3531 |
| BspMAI | CTGCAG | 1 cut(s) | 2116 |
| BspMI | ACCTGC | 2 cut(s) | 289, 2285 |
| BspOI | GCTAGC | 1 cut(s) | 2637 |
| BspPI | GGATC | 6 cut(s) | 865, 1028, 1235, 1748, 2018, 2366 |
| BspQI | GCTCTTC | 2 cut(s) | 1405, 2025 |
| BspT104I | TTCGAA | 1 cut(s) | 2668 |
| BspT107I | GGYRCC | 1 cut(s) | 2390 |
| BspTNI | GGTCTC | 2 cut(s) | 307, 1001 |
| BsrBI | CCGCTC | 1 cut(s) | 1164 |
| BsrDI | GCAATG | 5 cut(s) | 924, 2106, 3387, 3431, 3507 |
| BsrFI | RCCGGY | 2 cut(s) | 1823, 1923 |
| BsrI | ACTGG | 5 cut(s) | 325, 1054, 2522, 3281, 3573 |
| BssAI | RCCGGY | 2 cut(s) | 1823, 1923 |
| BssECI | CCNNGG | 3 cut(s) | 462, 552, 2248 |
| BssT1I | CCWWGG | 3 cut(s) | 462, 552, 2248 |
| Bst6I | CTCTTC | 3 cut(s) | 1392, 1405, 2025 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 2651, 3410, 3572 |
| BstBI | TTCGAA | 1 cut(s) | 2668 |
| BstC8I | GCNNGC | 9 cut(s) | 635, 642, 658, 1425, 1925, 2635, 2859, 3028, 3359 |
| BstDSI | CCRYGG | 1 cut(s) | 462 |
| BstF5I | GGATG | 6 cut(s) | 318, 545, 2244, 2713, 3106, 3577 |
| BstFNI | CGCG | 1 cut(s) | 633 |
| BstH2I | RGCGCY | 1 cut(s) | 1581 |
| BstHHI | GCGC | 2 cut(s) | 1580, 3646 |
| BstMAI | GTCTC | 5 cut(s) | 252, 307, 963, 1001, 3111 |
| BstNSI | RCATGY | 3 cut(s) | 1157, 3074, 3371 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 173, 3228 |
| BstSFI | CTRYAG | 4 cut(s) | 663, 1647, 1787, 2112 |
| BstSLI | GKGCMC | 2 cut(s) | 2349, 3568 |
| BstUI | CGCG | 1 cut(s) | 633 |
| BstV2I | GAAGAC | 3 cut(s) | 169, 2211, 2403 |
| BstX2I | RGATCY | 2 cut(s) | 1131, 1753 |
| BstYI | RGATCY | 2 cut(s) | 1131, 1753 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1458 |
| BsuI | GTATCC | 1 cut(s) | 1119 |
| BsuRI | GGCC | 6 cut(s) | 282, 894, 1120, 1923, 2063, 3198 |
| BtgI | CCRYGG | 1 cut(s) | 462 |
| BtgZI | GCGATG | 2 cut(s) | 1646, 3036 |
| BtsCI | GGATG | 6 cut(s) | 318, 545, 2244, 2713, 3106, 3577 |
| BtsI | GCAGTG | 1 cut(s) | 1704 |
| BtsIMutI | CAGTG | 4 cut(s) | 1704, 3195, 3566, 3741 |
| BveI | ACCTGC | 2 cut(s) | 289, 2285 |
| Cac8I | GCNNGC | 9 cut(s) | 635, 642, 658, 1425, 1925, 2635, 2859, 3028, 3359 |
| CaiI | CAGNNNCTG | 3 cut(s) | 656, 748, 1250 |
| CciI | TCATGA | 1 cut(s) | 165 |
| CfoI | GCGC | 2 cut(s) | 1580, 3646 |
| Cfr10I | RCCGGY | 2 cut(s) | 1823, 1923 |
| Cfr13I | GGNCC | 4 cut(s) | 892, 2732, 3339, 3529 |
| CseI | GACGC | 1 cut(s) | 178 |
| CspAI | ACCGGT | 1 cut(s) | 1823 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 778, 813, 1680, 1715 |
| DraIII | CACNNNGTG | 1 cut(s) | 2192 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 955 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 955 |
| EaeI | YGGCCR | 2 cut(s) | 1921, 2061 |
| Eam1104I | CTCTTC | 3 cut(s) | 1392, 1405, 2025 |
| EarI | CTCTTC | 3 cut(s) | 1392, 1405, 2025 |
| Eco130I | CCWWGG | 3 cut(s) | 462, 552, 2248 |
| Eco147I | AGGCCT | 1 cut(s) | 282 |
| Eco24I | GRGCYC | 1 cut(s) | 1417 |
| Eco31I | GGTCTC | 2 cut(s) | 307, 1001 |
| Eco47I | GGWCC | 3 cut(s) | 2732, 3339, 3529 |
| Eco47III | AGCGCT | 1 cut(s) | 1579 |
| Eco57I | CTGAAG | 2 cut(s) | 2004, 3396 |
| Eco81I | CCTNAGG | 1 cut(s) | 1458 |
| Eco88I | CYCGRG | 1 cut(s) | 3013 |
| EcoO109I | RGGNCCY | 2 cut(s) | 2732, 3529 |
| EcoRI | GAATTC | 2 cut(s) | 342, 1551 |
| EcoT14I | CCWWGG | 3 cut(s) | 462, 552, 2248 |
| EcoT22I | ATGCAT | 3 cut(s) | 970, 2818, 3760 |
| EcoT38I | GRGCYC | 1 cut(s) | 1417 |
| ErhI | CCWWGG | 3 cut(s) | 462, 552, 2248 |
| Esp3I | CGTCTC | 2 cut(s) | 963, 3111 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 528, 560, 1602, 1634, 2395, 2427, 2559, 2591 |
| FaqI | GGGAC | 3 cut(s) | 212, 452, 2718 |
| FauI | CCCGC | 2 cut(s) | 626, 2383 |
| FauNDI | CATATG | 1 cut(s) | 1375 |
| FbaI | TGATCA | 3 cut(s) | 46, 117, 867 |
| FblI | GTMKAC | 1 cut(s) | 3185 |
| FokI | GGATG | 6 cut(s) | 325, 552, 2251, 2720, 3113, 3584 |
| FriOI | GRGCYC | 1 cut(s) | 1417 |
| FspI | TGCGCA | 1 cut(s) | 3645 |
| GlaI | GCGC | 2 cut(s) | 1579, 3645 |
| GsaI | CCCAGC | 1 cut(s) | 218 |
| GsuI | CTGGAG | 4 cut(s) | 612, 1071, 2244, 3504 |
| HaeII | RGCGCY | 1 cut(s) | 1581 |
| HaeIII | GGCC | 6 cut(s) | 282, 894, 1120, 1923, 2063, 3198 |
| HapII | CCGG | 5 cut(s) | 411, 711, 1824, 1924, 2267 |
| HgaI | GACGC | 1 cut(s) | 178 |
| HhaI | GCGC | 2 cut(s) | 1580, 3646 |
| Hin6I | GCGC | 2 cut(s) | 1578, 3644 |
| HinP1I | GCGC | 2 cut(s) | 1578, 3644 |
| HincII | GTYRAC | 3 cut(s) | 627, 1209, 3186 |
| HindII | GTYRAC | 3 cut(s) | 627, 1209, 3186 |
| HpaII | CCGG | 5 cut(s) | 411, 711, 1824, 1924, 2267 |
| HphI | GGTGA | 7 cut(s) | 328, 541, 945, 1265, 2456, 2713, 3631 |
| Hpy166II | GTNNAC | 8 cut(s) | 627, 1209, 1502, 1932, 2347, 3186, 3236, 3566 |
| Hpy8I | GTNNAC | 8 cut(s) | 627, 1209, 1502, 1932, 2347, 3186, 3236, 3566 |
| Hpy99I | CGWCG | 3 cut(s) | 1568, 1742, 3554 |
| HpyAV | CCTTC | 7 cut(s) | 712, 1754, 1787, 2425, 2692, 2723, 3706 |
| HpyCH4IV | ACGT | 2 cut(s) | 110, 3120 |
| HpySE526I | ACGT | 2 cut(s) | 110, 3120 |
| HspAI | GCGC | 2 cut(s) | 1578, 3644 |
| KroI | GCCGGC | 1 cut(s) | 1923 |
| KroNI | GCCGGC | 1 cut(s) | 1925 |
| Ksp22I | TGATCA | 3 cut(s) | 46, 117, 867 |
| LguI | GCTCTTC | 2 cut(s) | 1405, 2025 |
| LmnI | GCTCC | 9 cut(s) | 203, 498, 683, 1763, 1796, 1872, 2102, 2834, 2944 |
| MaeII | ACGT | 2 cut(s) | 110, 3120 |
| MaeIII | GTNAC | 4 cut(s) | 316, 1978, 2444, 3370 |
| MbiI | CCGCTC | 1 cut(s) | 1164 |
| MfeI | CAATTG | 4 cut(s) | 579, 1029, 1521, 2655 |
| MflI | RGATCY | 2 cut(s) | 1131, 1753 |
| MhlI | GDGCHC | 3 cut(s) | 1417, 2349, 3568 |
| MlsI | TGGCCA | 1 cut(s) | 2063 |
| MluNI | TGGCCA | 1 cut(s) | 2063 |
| MlyI | GAGTC | 9 cut(s) | 606, 1281, 1601, 2201, 2939, 2957, 3005, 3218, 3587 |
| MmeI | TCCRAC | 7 cut(s) | 290, 661, 778, 2973, 3078, 3142, 3561 |
| Mox20I | TGGCCA | 1 cut(s) | 2063 |
| Mph1103I | ATGCAT | 3 cut(s) | 970, 2818, 3760 |
| MroNI | GCCGGC | 1 cut(s) | 1923 |
| MroXI | GAANNNNTTC | 3 cut(s) | 2279, 3315, 3333 |
| MscI | TGGCCA | 1 cut(s) | 2063 |
| MslI | CAYNNNNRTG | 2 cut(s) | 113, 2171 |
| Msp20I | TGGCCA | 1 cut(s) | 2063 |
| MspA1I | CMGCKG | 1 cut(s) | 2117 |
| MspI | CCGG | 5 cut(s) | 411, 711, 1824, 1924, 2267 |
| MunI | CAATTG | 4 cut(s) | 579, 1029, 1521, 2655 |
| Mva1269I | GAATGC | 1 cut(s) | 2664 |
| MvnI | CGCG | 1 cut(s) | 633 |
| NaeI | GCCGGC | 1 cut(s) | 1925 |
| NcoI | CCATGG | 1 cut(s) | 462 |
| NdeI | CATATG | 1 cut(s) | 1375 |
| NgoMIV | GCCGGC | 1 cut(s) | 1923 |
| NheI | GCTAGC | 1 cut(s) | 2633 |
| NlaIV | GGNNCC | 8 cut(s) | 685, 893, 1874, 2104, 2392, 2734, 2940, 3531 |
| NmuCI | GTSAC | 3 cut(s) | 316, 1978, 2444 |
| NsbI | TGCGCA | 1 cut(s) | 3645 |
| NsiI | ATGCAT | 3 cut(s) | 970, 2818, 3760 |
| NspI | RCATGY | 3 cut(s) | 1157, 3074, 3371 |
| NspV | TTCGAA | 1 cut(s) | 2668 |
| PaeR7I | CTCGAG | 1 cut(s) | 3013 |
| PagI | TCATGA | 1 cut(s) | 165 |
| PaqCI | CACCTGC | 1 cut(s) | 2285 |
| PceI | AGGCCT | 1 cut(s) | 282 |
| PciI | ACATGT | 2 cut(s) | 3070, 3367 |
| PciSI | GCTCTTC | 2 cut(s) | 1405, 2025 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 637 |
| PctI | GAATGC | 1 cut(s) | 2664 |
| PdiI | GCCGGC | 1 cut(s) | 1925 |
| PdmI | GAANNNNTTC | 3 cut(s) | 2279, 3315, 3333 |
| PfeI | GAWTC | 6 cut(s) | 1143, 1817, 2924, 2986, 3210, 3329 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1250 |
| PinAI | ACCGGT | 1 cut(s) | 1823 |
| PleI | GAGTC | 9 cut(s) | 605, 1280, 1600, 2201, 2939, 2957, 3005, 3218, 3586 |
| PpsI | GAGTC | 9 cut(s) | 605, 1280, 1600, 2201, 2939, 2957, 3005, 3218, 3586 |
| PpuMI | RGGWCCY | 2 cut(s) | 2732, 3529 |
| PscI | ACATGT | 2 cut(s) | 3070, 3367 |
| PshAI | GACNNNNGTC | 2 cut(s) | 173, 3228 |
| PshBI | ATTAAT | 2 cut(s) | 1628, 2819 |
| Psp5II | RGGWCCY | 2 cut(s) | 2732, 3529 |
| PspFI | CCCAGC | 1 cut(s) | 214 |
| PspN4I | GGNNCC | 8 cut(s) | 685, 893, 1874, 2104, 2392, 2734, 2940, 3531 |
| PspPI | GGNCC | 4 cut(s) | 892, 2732, 3339, 3529 |
| PspPPI | RGGWCCY | 2 cut(s) | 2732, 3529 |
| PspXI | VCTCGAGB | 1 cut(s) | 3013 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 3576, 3608 |
| PstI | CTGCAG | 1 cut(s) | 2116 |
| PstNI | CAGNNNCTG | 3 cut(s) | 656, 748, 1250 |
| PsuI | RGATCY | 2 cut(s) | 1131, 1753 |
| PvuII | CAGCTG | 1 cut(s) | 2117 |
| RseI | CAYNNNNRTG | 2 cut(s) | 113, 2171 |
| SalI | GTCGAC | 1 cut(s) | 3184 |
| SapI | GCTCTTC | 2 cut(s) | 1405, 2025 |
| Sau96I | GGNCC | 4 cut(s) | 892, 2732, 3339, 3529 |
| ScaI | AGTACT | 1 cut(s) | 2574 |
| SchI | GAGTC | 9 cut(s) | 606, 1281, 1601, 2201, 2939, 2957, 3005, 3218, 3587 |
| SduI | GDGCHC | 3 cut(s) | 1417, 2349, 3568 |
| SfcI | CTRYAG | 4 cut(s) | 663, 1647, 1787, 2112 |
| Sfr274I | CTCGAG | 1 cut(s) | 3013 |
| SfuI | TTCGAA | 1 cut(s) | 2668 |
| SinI | GGWCC | 3 cut(s) | 2732, 3339, 3529 |
| SlaI | CTCGAG | 1 cut(s) | 3013 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 113, 2171 |
| SmlI | CTYRAG | 4 cut(s) | 240, 399, 1004, 3013 |
| SmoI | CTYRAG | 4 cut(s) | 240, 399, 1004, 3013 |
| SseBI | AGGCCT | 1 cut(s) | 282 |
| SsiI | CCGC | 5 cut(s) | 513, 633, 1164, 2376, 3649 |
| SspI | AATATT | 2 cut(s) | 2979, 3346 |
| StuI | AGGCCT | 1 cut(s) | 282 |
| StyI | CCWWGG | 3 cut(s) | 462, 552, 2248 |
| TaiI | ACGT | 2 cut(s) | 113, 3123 |
| TatI | WGTACW | 5 cut(s) | 261, 354, 2572, 3235, 3592 |
| TauI | GCSGC | 1 cut(s) | 1167 |
| TfiI | GAWTC | 6 cut(s) | 1143, 1817, 2924, 2986, 3210, 3329 |
| TscAI | CASTG | 4 cut(s) | 1704, 3195, 3573, 3748 |
| TseFI | GTSAC | 3 cut(s) | 316, 1978, 2444 |
| Tsp45I | GTSAC | 3 cut(s) | 316, 1978, 2444 |
| TspRI | CASTG | 4 cut(s) | 1704, 3195, 3573, 3748 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1250 |
| VneI | GTGCAC | 2 cut(s) | 2345, 3564 |
| VpaK11BI | GGWCC | 3 cut(s) | 2732, 3339, 3529 |
| VspI | ATTAAT | 2 cut(s) | 1628, 2819 |
| XapI | RAATTY | 8 cut(s) | 342, 1551, 2275, 2316, 2380, 2585, 2864, 3537 |
| XbaI | TCTAGA | 2 cut(s) | 1582, 1828 |
| XceI | RCATGY | 3 cut(s) | 1157, 3074, 3371 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2056 |
| XhoI | CTCGAG | 1 cut(s) | 3013 |
| XmiI | GTMKAC | 1 cut(s) | 3185 |
| XmnI | GAANNNNTTC | 3 cut(s) | 2279, 3315, 3333 |
| ZrmI | AGTACT | 1 cut(s) | 2574 |
| Zsp2I | ATGCAT | 3 cut(s) | 970, 2818, 3760 |