Rroxscaffold_4G00325550

Serine threonine-protein phosphatase 2A 65 kDa regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001174
Physical Location & Seq
Forward (+)
57292603 .. 57296756
4154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325550.1

Sequence Viewer

Length: 1038 bp
ATGAAAGACTTACATAGGGATGATCGGCATGAGGAGGAGATCACGAAAATGGGGTGGGTCGGCCTTGGGGACGCCGGAAAACACACTTTCCGGCCGGTCGGGTCGGCGGAGGCCGGGGAGCCTTCGATCCGCACTTACGGGCGGCGGCGGTGCAAGGGAGGACCAGAGAGAGGTCCGGCGTCGAGCGGCGATCCTAGGGATGGCGGGTCGCCGCGGAGGAGGCCGGAGAAGAGCGTTATGGCCGGGTGGAGAAAGCGGAGTTTAATGGAATTGTCAACAGATTCATCCCGGCACGTTCGCTCTGCATTGGCTTCGGTGATAATGGGAATGGCACCAGTTTTAGGAAAGGCAAGAATCTTCTATGAGTTTCCGGATGTCCGGCTGAACATTATTAGCAAGCTTGATCAAGTCAACCAGCCGTTCTTGGTAATTTACCTGGTGCGGGGAATTGGAATTGATCTGCTGTGTCCCGGTCTCTCTGCCAGCAATTGTGGAACCGGAGAGGATAGACATTGGAGGGTTCAGCTTGCGATAATAGAATATATCCCTTTATTGGCAAGTCAGTTGGGTGTGGGGTTTTTCGATGATAAGCTTGGTTCTCTTTGCATGCAGTGGTTAAATGATAAGGTTTACTCAATACGTGATGCAGCTGCTAATAATGTGAAGCGCCTTGCAGAAGAATTTGGTCCTGATTGGGCCATGCAGCATATAGTCCCACAGGTTTTGGAAATGATTAATAACCCTCACTATTTGTATCGGATGACCATTCTACATGCAATTTCCCTACTTGCCCCTGTTATGGGATCAGAAATTACTTGTTCCAAACTTCTACCGGTGGTTATCACTGCATCAAAAGATAGGTATTGCGTTCCGGATATTTGGATACTATCCGGAGGCAAAGGAAGTGATTGTCATAAGGCTGCAATTACGGGAGACATAGTGGGAGACCCATTCAAAGACACAGCTAGACCTTCACTTCATGTCCTTATTAAAATGCTTGCTACAAAAATGTTGGTCATGGCTCCTGTCTTTCATTGA

Protein Analysis

345

Amino Acids

38.18

Weight (kDa)

9.01

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP2R1A-like_HEAT PF22646 90 - 115 4.4e-06 PPP2R1A-like HEAT repeat
H_PPase PF03030 298 - 340 3.3e-15 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 331
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 214
AccIII TCCGGA 3 cut(s) 370, 871, 890
AclWI GGATC 3 cut(s) 121, 185, 811
AcoI YGGCCR 2 cut(s) 92, 240
AcsI RAATTY 1 cut(s) 680
AcyI GRCGYC 2 cut(s) 72, 179
AfiI CCNNNNNNNGG 7 cut(s) 170, 200, 341, 442, 553, 799, 800
AgeI ACCGGT 1 cut(s) 832
AgsI TTSAA 1 cut(s) 955
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 5 cut(s) 400, 526, 592, 650, 965
AluI AGCT 5 cut(s) 400, 526, 592, 650, 965
Alw26I GTCTC 3 cut(s) 479, 927, 939
AlwI GGATC 3 cut(s) 121, 185, 811
Aor13HI TCCGGA 3 cut(s) 370, 871, 890
AoxI GGCC 6 cut(s) 61, 92, 111, 221, 240, 696
ApeKI GCWGC 4 cut(s) 647, 650, 703, 920
ApoI RAATTY 1 cut(s) 680
AseI ATTAAT 1 cut(s) 735
AsiGI ACCGGT 1 cut(s) 832
AspA2I CCTAGG 1 cut(s) 194
AspLEI GCGC 1 cut(s) 669
AspS9I GGNCC 4 cut(s) 161, 173, 686, 696
AsuC2I CCSGG 4 cut(s) 115, 244, 289, 471
AsuHPI GGTGA 1 cut(s) 328
AvaII GGWCC 3 cut(s) 161, 173, 686
AvrII CCTAGG 1 cut(s) 194
BanI GGYRCC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 637, 659, 715, 907
BccI CCATC 1 cut(s) 194
BceAI ACGGC 1 cut(s) 403
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 1 cut(s) 437
BciVI GTATCC 1 cut(s) 876
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 4 cut(s) 115, 244, 289, 471
BcoDI GTCTC 3 cut(s) 479, 927, 939
BfaI CTAG 2 cut(s) 195, 966
BfoI RGCGCY 1 cut(s) 670
BfuI GTATCC 1 cut(s) 876
BisI GCNGC 8 cut(s) 143, 146, 187, 212, 648, 651, 704, 921
BlnI CCTAGG 1 cut(s) 194
BlsI GCNGC 8 cut(s) 144, 147, 188, 213, 649, 652, 705, 922
Bme1390I CCNGG 5 cut(s) 115, 244, 289, 437, 471
Bme18I GGWCC 3 cut(s) 161, 173, 686
BmgT120I GGNCC 4 cut(s) 161, 173, 686, 696
BmiI GGNNCC 4 cut(s) 120, 333, 496, 1023
BmrFI CCNGG 5 cut(s) 115, 244, 289, 437, 471
BmsI GCATC 2 cut(s) 634, 857
BpuMI CCSGG 4 cut(s) 115, 244, 289, 471
BsaAI YACGTR 1 cut(s) 641
BsaHI GRCGYC 2 cut(s) 72, 179
BsaI GGTCTC 2 cut(s) 479, 939
BsaJI CCNNGG 4 cut(s) 64, 114, 194, 212
BsaWI WCCGGW 5 cut(s) 370, 497, 832, 871, 890
BsaXI ACNNNNNCTCC 2 cut(s) 924, 954
Bsc4I CCNNNNNNNGG 7 cut(s) 170, 200, 341, 442, 553, 799, 800
Bse118I RCCGGY 2 cut(s) 94, 832
Bse1I ACTGG 1 cut(s) 335
BseAI TCCGGA 3 cut(s) 370, 871, 890
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 4 cut(s) 64, 114, 194, 212
BseGI GGATG 5 cut(s) 25, 205, 284, 379, 765
BseLI CCNNNNNNNGG 7 cut(s) 170, 200, 341, 442, 553, 799, 800
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 3 cut(s) 47, 50, 232
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 4 cut(s) 637, 659, 715, 907
Bsh1236I CGCG 1 cut(s) 214
Bsh1285I CGRYCG 2 cut(s) 95, 99
BshFI GGCC 6 cut(s) 63, 94, 113, 223, 242, 698
BshNI GGYRCC 1 cut(s) 331
BshTI ACCGGT 1 cut(s) 832
BsiEI CGRYCG 2 cut(s) 95, 99
BslFI GGGAC 3 cut(s) 83, 453, 698
BslI CCNNNNNNNGG 7 cut(s) 170, 200, 341, 442, 553, 799, 800
BsmAI GTCTC 3 cut(s) 479, 927, 939
BsmFI GGGAC 3 cut(s) 83, 453, 698
BsnI GGCC 6 cut(s) 63, 94, 113, 223, 242, 698
Bso31I GGTCTC 2 cut(s) 479, 939
Bsp13I TCCGGA 3 cut(s) 370, 871, 890
Bsp143I GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
BspANI GGCC 6 cut(s) 63, 94, 113, 223, 242, 698
BspEI TCCGGA 3 cut(s) 370, 871, 890
BspFNI CGCG 1 cut(s) 214
BspLI GGNNCC 4 cut(s) 120, 333, 496, 1023
BspPI GGATC 3 cut(s) 121, 185, 811
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 331
BspTNI GGTCTC 2 cut(s) 479, 939
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 2 cut(s) 94, 832
BsrI ACTGG 1 cut(s) 335
BssAI RCCGGY 2 cut(s) 94, 832
BssECI CCNNGG 4 cut(s) 64, 114, 194, 212
BssMI GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
BssNI GRCGYC 2 cut(s) 72, 179
BssT1I CCWWGG 2 cut(s) 64, 194
Bst2UI CCWGG 1 cut(s) 437
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 2 cut(s) 72, 179
BstBAI YACGTR 1 cut(s) 641
BstC8I GCNNGC 5 cut(s) 398, 484, 528, 608, 999
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 5 cut(s) 25, 205, 284, 379, 765
BstFNI CGCG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 670
BstHHI GCGC 1 cut(s) 669
BstKTI GATC 7 cut(s) 25, 42, 129, 193, 406, 460, 806
BstMAI GTCTC 3 cut(s) 479, 927, 939
BstMBI GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
BstMCI CGRYCG 2 cut(s) 95, 99
BstMWI GCNNNNNNNGC 1 cut(s) 220
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 2 cut(s) 610, 776
BstSCI CCNGG 5 cut(s) 113, 242, 287, 435, 469
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 4 cut(s) 637, 659, 715, 907
BstZI CGGCCG 1 cut(s) 92
BsuI GTATCC 1 cut(s) 876
BsuRI GGCC 6 cut(s) 63, 94, 113, 223, 242, 698
BtgI CCRYGG 1 cut(s) 212
BtsCI GGATG 5 cut(s) 25, 205, 284, 379, 765
BtsI GCAGTG 2 cut(s) 617, 843
BtsIMutI CAGTG 2 cut(s) 617, 843
Cac8I GCNNGC 5 cut(s) 398, 484, 528, 608, 999
CfoI GCGC 1 cut(s) 669
Cfr10I RCCGGY 2 cut(s) 94, 832
Cfr13I GGNCC 4 cut(s) 161, 173, 686, 696
Cfr42I CCGCGG 1 cut(s) 215
CseI GACGC 2 cut(s) 80, 168
CsiI ACCWGGT 1 cut(s) 435
CspAI ACCGGT 1 cut(s) 832
CspCI CAANNNNNGTGG 2 cut(s) 705, 740
CviAII CATG 6 cut(s) 29, 607, 700, 773, 980, 1018
DpnI GATC 7 cut(s) 24, 41, 128, 192, 405, 459, 805
DpnII GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
EaeI YGGCCR 2 cut(s) 92, 240
EagI CGGCCG 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 122
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 2 cut(s) 64, 194
Eco31I GGTCTC 2 cut(s) 479, 939
Eco47I GGWCC 3 cut(s) 161, 173, 686
Eco52I CGGCCG 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 64, 194
ErhI CCWWGG 2 cut(s) 64, 194
FaeI CATG 6 cut(s) 32, 610, 703, 776, 983, 1021
FaqI GGGAC 3 cut(s) 83, 453, 698
FatI CATG 6 cut(s) 28, 606, 699, 772, 979, 1017
FauI CCCGC 2 cut(s) 197, 435
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 8 cut(s) 143, 146, 187, 212, 648, 651, 704, 921
FokI GGATG 5 cut(s) 32, 212, 271, 386, 772
Fsp4HI GCNGC 8 cut(s) 143, 146, 187, 212, 648, 651, 704, 921
FspBI CTAG 2 cut(s) 195, 966
GlaI GCGC 1 cut(s) 668
GluI GCNGC 8 cut(s) 143, 146, 187, 212, 648, 651, 704, 921
HaeII RGCGCY 1 cut(s) 670
HaeIII GGCC 6 cut(s) 63, 94, 113, 223, 242, 698
HgaI GACGC 2 cut(s) 80, 168
HhaI GCGC 1 cut(s) 669
Hin1I GRCGYC 2 cut(s) 72, 179
Hin1II CATG 6 cut(s) 32, 610, 703, 776, 983, 1021
Hin6I GCGC 1 cut(s) 667
HinP1I GCGC 1 cut(s) 667
HincII GTYRAC 2 cut(s) 276, 412
HindII GTYRAC 2 cut(s) 276, 412
HindIII AAGCTT 2 cut(s) 398, 590
HinfI GANTC 2 cut(s) 281, 354
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 3 cut(s) 276, 412, 631
Hpy188I TCNGA 2 cut(s) 759, 808
Hpy188III TCNNGA 5 cut(s) 43, 371, 689, 872, 891
Hpy8I GTNNAC 3 cut(s) 276, 412, 631
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 2 cut(s) 132, 981
HpyCH4IV ACGT 2 cut(s) 294, 640
HpyF10VI GCNNNNNNNGC 1 cut(s) 220
HpySE526I ACGT 2 cut(s) 294, 640
Hsp92I GRCGYC 2 cut(s) 72, 179
Hsp92II CATG 6 cut(s) 32, 610, 703, 776, 983, 1021
HspAI GCGC 1 cut(s) 667
Kpn2I TCCGGA 3 cut(s) 370, 871, 890
Ksp22I TGATCA 1 cut(s) 403
KspI CCGCGG 1 cut(s) 215
Kzo9I GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 2 cut(s) 118, 1027
Lsp1109I GCAGC 4 cut(s) 637, 659, 715, 907
LweI GCATC 2 cut(s) 634, 857
MabI ACCWGGT 1 cut(s) 435
MaeI CTAG 2 cut(s) 195, 966
MaeII ACGT 2 cut(s) 294, 640
MalI GATC 7 cut(s) 24, 41, 128, 192, 405, 459, 805
MbiI CCGCTC 1 cut(s) 186
MboI GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
MboII GAAGA 3 cut(s) 241, 349, 689
MfeI CAATTG 1 cut(s) 487
MluCI AATT 9 cut(s) 269, 429, 447, 453, 487, 680, 777, 810, 924
MroI TCCGGA 3 cut(s) 370, 871, 890
MseI TTAA 4 cut(s) 263, 617, 735, 990
MslI CAYNNNNRTG 2 cut(s) 18, 47
MspA1I CMGCKG 2 cut(s) 214, 650
MspR9I CCNGG 5 cut(s) 115, 244, 289, 437, 471
MunI CAATTG 1 cut(s) 487
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 1 cut(s) 220
NciI CCSGG 4 cut(s) 115, 244, 289, 471
NdeII GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
NlaIII CATG 6 cut(s) 32, 610, 703, 776, 983, 1021
NlaIV GGNNCC 4 cut(s) 120, 333, 496, 1023
NspI RCATGY 2 cut(s) 610, 776
PaeI GCATGC 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 224
PfeI GAWTC 2 cut(s) 281, 354
PinAI ACCGGT 1 cut(s) 832
PkrI GCNGC 8 cut(s) 144, 147, 188, 213, 649, 652, 705, 922
Ppu21I YACGTR 1 cut(s) 641
PshBI ATTAAT 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 4 cut(s) 120, 333, 496, 1023
PspPI GGNCC 4 cut(s) 161, 173, 686, 696
PvuII CAGCTG 1 cut(s) 650
RseI CAYNNNNRTG 2 cut(s) 18, 47
SacII CCGCGG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 4 cut(s) 263, 617, 735, 990
SatI GCNGC 8 cut(s) 143, 146, 187, 212, 648, 651, 704, 921
Sau3AI GATC 7 cut(s) 22, 39, 126, 190, 403, 457, 803
Sau96I GGNCC 4 cut(s) 161, 173, 686, 696
ScrFI CCNGG 5 cut(s) 115, 244, 289, 437, 471
SexAI ACCWGGT 1 cut(s) 435
SfaNI GCATC 2 cut(s) 634, 857
Sfr303I CCGCGG 1 cut(s) 215
SgrBI CCGCGG 1 cut(s) 215
SinI GGWCC 3 cut(s) 161, 173, 686
SmiMI CAYNNNNRTG 2 cut(s) 18, 47
SphI GCATGC 1 cut(s) 610
Sse9I AATT 9 cut(s) 269, 429, 447, 453, 487, 680, 777, 810, 924
SspMI CTAG 2 cut(s) 195, 966
StyD4I CCNGG 5 cut(s) 113, 242, 287, 435, 469
StyI CCWWGG 2 cut(s) 64, 194
TaiI ACGT 2 cut(s) 297, 643
TaqI TCGA 3 cut(s) 125, 182, 582
TasI AATT 9 cut(s) 269, 429, 447, 453, 487, 680, 777, 810, 924
TauI GCSGC 4 cut(s) 145, 148, 189, 214
TfiI GAWTC 2 cut(s) 281, 354
Tru1I TTAA 4 cut(s) 263, 617, 735, 990
Tru9I TTAA 4 cut(s) 263, 617, 735, 990
TscAI CASTG 2 cut(s) 617, 850
TseI GCWGC 4 cut(s) 647, 650, 703, 920
TspDTI ATGAA 4 cut(s) 17, 273, 968, 1022
TspRI CASTG 2 cut(s) 617, 850
VpaK11BI GGWCC 3 cut(s) 161, 173, 686
VspI ATTAAT 1 cut(s) 735
XapI RAATTY 1 cut(s) 680
XceI RCATGY 2 cut(s) 610, 776
XmaJI CCTAGG 1 cut(s) 194
XspI CTAG 2 cut(s) 195, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.