MD08G1233200.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0021180
Physical Location & Seq
Forward (+)
29976369 .. 29979810
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1233200.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGCTTCCAGTACCCAAACAGCGGCCAAAATTCCAGTAGTAGATTTTTCCCAAGAGGACTGCTGGAAGTCAGGGACAAGTTCTTGGCTCTCAGTACGCAGAGACATTTGTAATGCTCTCGAAGAGTTTGGCTGTTTCCTGGCAGTCCTTCCCAACAAAGTTTCCCGGGAGCTTAATGACACCATGTTTGGGACATTCAACGAGTTGTTTGATTTCCCCGACGAAATTAAAGCGCAATTTCCTCAGGAAAAACCTCTAGTTGAGGGTTACGTAAGGCTGCCTACCCGTGAATACATGTCATTTCATTCAAGTACTGATAAAATAAAGAACGTCACACGTCAACTTTGGCCGGAAGGAAATGACCAGTTTCGTGAGAGTGCTGATCTGTATTCAAAAGTGATGACAGAATTAAATCAAGTTGTGACGAAAATGGTATTTGAAAACTACAAGGTAGAGAAATACCATGATGATCACATTCAATCGACCATGCCCATTACCGTACTTGTAAAAGATGATGAACCCAAGACAGATGAAACTGAAACTGTTGAACACAATCATACAGACAAGTGTTTTACTGGCATACTCCATCAAGATACGTATGGTGTTGAGCTACGCTCAAAAGATAACGAGTGGATTGGTTATGATCCTCTGCCTTCATCGTTATTATTCCTAGCGGGTGATGGACTTCAGGTTTGGAGTAACGACAGAATAAAATCTTGTATGCACAGAGTCGTTTTGAAGGAAAATAAGGTAAGATACTCGTTCGGACAATTCTTTTGGAACAAAGGAGTGATATATGTACCAAATGAGCTTACGGACAAGGACTACCCCATGCAGTATAAGCCAATAGACCTAGTGGAATATTTTCAATATTATTATGAAAATATGTCCACTGTGGGATTTGATTTTTCCGTCAAGGAATATTGTGGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

312

Amino Acids

36.28

Weight (kDa)

4.9

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 11 - 94 3.3e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 177 - 257 3e-14 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021180)

Species Orthologous Gene IDs
malus_domestica MD08G1233200.v1.1 MD08G1233400.v1.1 MD08G1233500.v1.1 MD08G1233600.v1.1
pyrus_communis pycom08g20230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 23, 674
AclWI GGATC 1 cut(s) 638
AcoI YGGCCR 2 cut(s) 24, 347
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 5 cut(s) 13, 96, 313, 501, 801
AfiI CCNNNNNNNGG 2 cut(s) 22, 189
AflIII ACRYGT 2 cut(s) 294, 335
AgsI TTSAA 8 cut(s) 199, 309, 393, 440, 479, 548, 739, 869
AjiI CACGTC 1 cut(s) 338
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 3 cut(s) 172, 610, 811
AluI AGCT 3 cut(s) 172, 610, 811
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 2 cut(s) 24, 347
ApeKI GCWGC 1 cut(s) 277
ApoI RAATTY 1 cut(s) 30
ArsI GACNNNNNNTTYG 6 cut(s) 170, 202, 675, 707, 759, 791
Asp700I GAANNNNTTC 1 cut(s) 864
AspLEI GCGC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 166, 167
AsuHPI GGTGA 1 cut(s) 689
AvaI CYCGRG 1 cut(s) 165
AxyI CCTNAGG 1 cut(s) 243
BbvI GCAGC 1 cut(s) 264
BccI CCATC 2 cut(s) 594, 674
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 2 cut(s) 166, 167
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 3 cut(s) 257, 671, 854
BisI GCNGC 2 cut(s) 24, 278
BlsI GCNGC 2 cut(s) 25, 279
BmcAI AGTACT 1 cut(s) 313
Bme1390I CCNGG 3 cut(s) 140, 166, 167
BmeT110I CYCGRG 1 cut(s) 165
BmgBI CACGTC 1 cut(s) 338
BmrFI CCNGG 3 cut(s) 140, 166, 167
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpuMI CCSGG 2 cut(s) 166, 167
BsaAI YACGTR 2 cut(s) 271, 597
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 189
Bse1I ACTGG 4 cut(s) 9, 35, 364, 580
Bse21I CCTNAGG 1 cut(s) 243
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 22, 189
BseMII CTCAG 2 cut(s) 105, 257
BseNI ACTGG 4 cut(s) 9, 35, 364, 580
BseXI GCAGC 1 cut(s) 264
BshFI GGCC 2 cut(s) 26, 349
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 2 cut(s) 166, 350
BslFI GGGAC 2 cut(s) 88, 205
BslI CCNNNNNNNGG 2 cut(s) 22, 189
BsmAI GTCTC 1 cut(s) 96
BsmFI GGGAC 2 cut(s) 88, 205
BsnI GGCC 2 cut(s) 26, 349
BsoBI CYCGRG 1 cut(s) 165
Bsp143I GATC 3 cut(s) 382, 469, 643
BspACI CCGC 2 cut(s) 23, 674
BspANI GGCC 2 cut(s) 26, 349
BspCNI CTCAG 2 cut(s) 104, 256
BspPI GGATC 1 cut(s) 638
BsrI ACTGG 4 cut(s) 9, 35, 364, 580
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 382, 469, 643
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 499, 544, 895
Bst6I CTCTTC 1 cut(s) 117
BstBAI YACGTR 2 cut(s) 271, 597
BstDEI CTNAG 2 cut(s) 91, 243
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 3 cut(s) 385, 472, 646
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 3 cut(s) 382, 469, 643
BstMWI GCNNNNNNNGC 1 cut(s) 841
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 3 cut(s) 138, 164, 165
BstSNI TACGTA 2 cut(s) 271, 597
BstV1I GCAGC 1 cut(s) 264
Bsu36I CCTNAGG 1 cut(s) 243
BsuRI GGCC 2 cut(s) 26, 349
BtrI CACGTC 1 cut(s) 338
BtsIMutI CAGTG 1 cut(s) 891
CfoI GCGC 1 cut(s) 235
Cfr9I CCCGGG 1 cut(s) 165
Csp6I GTAC 5 cut(s) 12, 95, 312, 500, 800
CviAII CATG 5 cut(s) 184, 295, 464, 487, 832
CviQI GTAC 5 cut(s) 12, 95, 312, 500, 800
DdeI CTNAG 2 cut(s) 91, 243
DpnI GATC 3 cut(s) 384, 471, 645
DpnII GATC 3 cut(s) 382, 469, 643
EaeI YGGCCR 2 cut(s) 24, 347
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco105I TACGTA 2 cut(s) 271, 597
Eco57I CTGAAG 1 cut(s) 671
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 138
FaeI CATG 5 cut(s) 187, 298, 467, 490, 835
FaqI GGGAC 2 cut(s) 88, 205
FatI CATG 5 cut(s) 183, 294, 463, 486, 831
FauI CCCGC 1 cut(s) 667
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 24, 278
Fsp4HI GCNGC 2 cut(s) 24, 278
FspBI CTAG 3 cut(s) 257, 671, 854
GlaI GCGC 1 cut(s) 234
GluI GCNGC 2 cut(s) 24, 278
HaeIII GGCC 2 cut(s) 26, 349
HapII CCGG 2 cut(s) 166, 350
HhaI GCGC 1 cut(s) 235
Hin1II CATG 5 cut(s) 187, 298, 467, 490, 835
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HincII GTYRAC 1 cut(s) 341
HindII GTYRAC 1 cut(s) 341
HinfI GANTC 1 cut(s) 729
HpaII CCGG 2 cut(s) 166, 350
HphI GGTGA 1 cut(s) 689
Hpy166II GTNNAC 2 cut(s) 341, 891
Hpy188I TCNGA 1 cut(s) 767
Hpy188III TCNNGA 4 cut(s) 119, 245, 371, 590
Hpy8I GTNNAC 2 cut(s) 341, 891
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 4 cut(s) 158, 347, 663, 733
HpyCH4III ACNGT 3 cut(s) 499, 544, 895
HpyCH4IV ACGT 4 cut(s) 270, 330, 337, 596
HpyCH4V TGCA 2 cut(s) 724, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 841
HpyF3I CTNAG 2 cut(s) 91, 243
HpySE526I ACGT 4 cut(s) 270, 330, 337, 596
Hsp92II CATG 5 cut(s) 187, 298, 467, 490, 835
HspAI GCGC 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 382, 469, 643
LmnI GCTCC 1 cut(s) 169
Lsp1109I GCAGC 1 cut(s) 264
MaeI CTAG 3 cut(s) 257, 671, 854
MaeII ACGT 4 cut(s) 270, 330, 337, 596
MaeIII GTNAC 4 cut(s) 266, 331, 421, 698
MalI GATC 3 cut(s) 384, 471, 645
MboI GATC 3 cut(s) 382, 469, 643
MboII GAAGA 1 cut(s) 134
MluCI AATT 5 cut(s) 30, 225, 236, 407, 770
MlyI GAGTC 1 cut(s) 738
MnlI CCTC 5 cut(s) 49, 252, 256, 264, 657
MroXI GAANNNNTTC 1 cut(s) 864
MseI TTAA 3 cut(s) 174, 228, 410
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 166, 350
MspR9I CCNGG 3 cut(s) 140, 166, 167
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 841
NciI CCSGG 2 cut(s) 166, 167
NdeII GATC 3 cut(s) 382, 469, 643
NlaIII CATG 5 cut(s) 187, 298, 467, 490, 835
NmuCI GTSAC 2 cut(s) 331, 421
NspI RCATGY 1 cut(s) 298
PciI ACATGT 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 864
PkrI GCNGC 2 cut(s) 25, 279
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
Ppu21I YACGTR 2 cut(s) 271, 597
PscI ACATGT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
RsaI GTAC 5 cut(s) 13, 96, 313, 501, 801
RsaNI GTAC 5 cut(s) 12, 95, 312, 500, 800
SaqAI TTAA 3 cut(s) 174, 228, 410
SatI GCNGC 2 cut(s) 24, 278
Sau3AI GATC 3 cut(s) 382, 469, 643
ScaI AGTACT 1 cut(s) 313
SchI GAGTC 1 cut(s) 738
ScrFI CCNGG 3 cut(s) 140, 166, 167
SmaI CCCGGG 1 cut(s) 167
SnaBI TACGTA 2 cut(s) 271, 597
Sse9I AATT 5 cut(s) 30, 225, 236, 407, 770
SsiI CCGC 2 cut(s) 23, 674
SspI AATATT 3 cut(s) 863, 872, 923
SspMI CTAG 3 cut(s) 257, 671, 854
StyD4I CCNGG 3 cut(s) 138, 164, 165
TaaI ACNGT 3 cut(s) 499, 544, 895
TaiI ACGT 4 cut(s) 273, 333, 340, 599
TaqI TCGA 2 cut(s) 120, 482
TasI AATT 5 cut(s) 30, 225, 236, 407, 770
TatI WGTACW 1 cut(s) 311
TauI GCSGC 1 cut(s) 26
Tru1I TTAA 3 cut(s) 174, 228, 410
Tru9I TTAA 3 cut(s) 174, 228, 410
TscAI CASTG 1 cut(s) 898
TseFI GTSAC 2 cut(s) 331, 421
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 2 cut(s) 331, 421
TspDTI ATGAA 5 cut(s) 293, 531, 546, 645, 894
TspGWI ACGGA 2 cut(s) 830, 901
TspMI CCCGGG 1 cut(s) 165
TspRI CASTG 1 cut(s) 898
XapI RAATTY 1 cut(s) 30
XceI RCATGY 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 3 cut(s) 257, 671, 854
ZrmI AGTACT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.