MD08G1233600.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0021180
Physical Location & Seq
Forward (+)
30046064 .. 30049505
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1233600.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCTTCCAGGACCCAAACAGCGACCAAAATTCCAGTAGTAGATTTCTCCCAAGAGGATTGCTGGAAGTCAGGGACAAGTTCTTGGCTCTCAGTACGCAGAGACGTTTGTAATGCTCTCGAAGAGTTTGCCTGTTTCCTGGCAGTCCTTCCCAACAAAGTTTCCCGGGAGCTTAATGACACCATGTTTGGGACATTCAACGAGTTGTTTGATTTCCCCGACGAAATTAAAGCACAATTTCCTCAGGATAAACCTCTAGTTGAGGGTTACGTAAGGCTACCTACCCGTGAATCCATGTCATTTCGTTCAAGCACTGAAAAAATAAAGAACGTCACACGTCAACTTTGGCCGGAAGGAAATGACCAGTTTCGTGAGAGTGCTGATCTGTATTCAAAAGTGATGACAGAATTAAATCAAGTTGTGACAAAAATGGTATTTGAAAACTACAACGTAGAGAAATACCACGATGATCACATCCAATCGACCATGCCCATTACCCTACTTATAAAATATGATGAACCCAAGACAAATGAAACTGAAACTGTTCTACACAATCATACAGACAAGTGTTTTACTACCATACTCCATCAAGAATCGTATGGTGTTGAGATAAGCACAAAAGATAACGAGTGGATTGGTTATGATCCTCTGCCTTCATCATTATTATTCCTAGCAGGTGATGGACTTCAGGTTTGGAGTAACGACAGAATAAAATCTTGTATGCACAGAGTCGTTTTGAAGGAAAATAAGGTAAGATACTCGTTCGGACAATTCTTTTGGAACAAAGGAGTGATGTATGTACCAAATGAGCTTACGGACGAGGACCACCCCATGCAGTATAAGCCAATAGACCTAGTGGAATATCTTCAATATTATCATGAAAATATGTCCACCGTGGGATTTGATTTTTCGGTCAAGGACTATTGTGGGAAGCATGACGCCACAAGCATGAGTTTGAAAGGTGGTGTTGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

326

Amino Acids

37.68

Weight (kDa)

5.2

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 11 - 95 1.9e-06 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 168 - 257 5.2e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021180)

Species Orthologous Gene IDs
malus_domestica MD08G1233200.v1.1 MD08G1233400.v1.1 MD08G1233500.v1.1 MD08G1233600.v1.1
pyrus_communis pycom08g20230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
AarI CACCTGC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 665
AclWI GGATC 1 cut(s) 638
AcoI YGGCCR 1 cut(s) 347
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 939
AfaI GTAC 2 cut(s) 96, 801
AfiI CCNNNNNNNGG 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 335
AgsI TTSAA 7 cut(s) 199, 309, 393, 440, 739, 869, 958
AjiI CACGTC 1 cut(s) 338
AjnI CCWGG 2 cut(s) 8, 138
AluBI AGCT 2 cut(s) 172, 811
AluI AGCT 2 cut(s) 172, 811
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 347
ApoI RAATTY 1 cut(s) 30
ArsI GACNNNNNNTTYG 6 cut(s) 170, 202, 675, 707, 759, 791
Asp700I GAANNNNTTC 2 cut(s) 543, 864
AspS9I GGNCC 2 cut(s) 12, 823
AsuC2I CCSGG 2 cut(s) 166, 167
AsuHPI GGTGA 1 cut(s) 689
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 2 cut(s) 12, 823
AxyI CCTNAGG 1 cut(s) 243
BccI CCATC 2 cut(s) 594, 674
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BciT130I CCWGG 2 cut(s) 10, 140
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 2 cut(s) 166, 167
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 3 cut(s) 257, 671, 854
BfuAI ACCTGC 1 cut(s) 665
Bme1390I CCNGG 4 cut(s) 10, 140, 166, 167
Bme18I GGWCC 2 cut(s) 12, 823
BmeT110I CYCGRG 1 cut(s) 165
BmgBI CACGTC 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 12, 823
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 4 cut(s) 10, 140, 166, 167
BpuMI CCSGG 2 cut(s) 166, 167
BsaAI YACGTR 1 cut(s) 271
BsaHI GRCGYC 1 cut(s) 939
BsaJI CCNNGG 2 cut(s) 165, 894
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 2 cut(s) 35, 364
Bse21I CCTNAGG 1 cut(s) 243
BseBI CCWGG 2 cut(s) 10, 140
BseDI CCNNGG 2 cut(s) 165, 894
BseGI GGATG 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 2 cut(s) 105, 257
BseNI ACTGG 2 cut(s) 35, 364
BshFI GGCC 1 cut(s) 349
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 2 cut(s) 166, 350
BslFI GGGAC 2 cut(s) 88, 205
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 2 cut(s) 88, 205
BsnI GGCC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 165
Bsp143I GATC 3 cut(s) 382, 469, 643
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 2 cut(s) 104, 256
BspHI TCATGA 1 cut(s) 877
BspLI GGNNCC 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 665
BspPI GGATC 1 cut(s) 638
BsrI ACTGG 2 cut(s) 35, 364
BssECI CCNNGG 2 cut(s) 165, 894
BssMI GATC 3 cut(s) 382, 469, 643
BssNI GRCGYC 1 cut(s) 939
Bst2UI CCWGG 2 cut(s) 10, 140
Bst4CI ACNGT 2 cut(s) 544, 895
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 939
BstBAI YACGTR 1 cut(s) 271
BstDEI CTNAG 2 cut(s) 91, 243
BstDSI CCRYGG 1 cut(s) 894
BstF5I GGATG 1 cut(s) 474
BstKTI GATC 3 cut(s) 385, 472, 646
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 3 cut(s) 382, 469, 643
BstMWI GCNNNNNNNGC 1 cut(s) 841
BstNI CCWGG 2 cut(s) 10, 140
BstSCI CCNGG 4 cut(s) 8, 138, 164, 165
BstSNI TACGTA 1 cut(s) 271
Bsu36I CCTNAGG 1 cut(s) 243
BsuRI GGCC 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 894
BtrI CACGTC 1 cut(s) 338
BtsCI GGATG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 312
BveI ACCTGC 1 cut(s) 665
CciI TCATGA 1 cut(s) 877
Cfr13I GGNCC 2 cut(s) 12, 823
Cfr9I CCCGGG 1 cut(s) 165
CseI GACGC 1 cut(s) 947
Csp6I GTAC 2 cut(s) 95, 800
CviAII CATG 7 cut(s) 184, 295, 487, 832, 878, 935, 949
CviJI RGCY 7 cut(s) 5, 88, 172, 277, 349, 811, 844
CviKI_1 RGCY 7 cut(s) 5, 88, 172, 277, 349, 811, 844
CviQI GTAC 2 cut(s) 95, 800
DdeI CTNAG 2 cut(s) 91, 243
DpnI GATC 3 cut(s) 384, 471, 645
DpnII GATC 3 cut(s) 382, 469, 643
EaeI YGGCCR 1 cut(s) 347
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco105I TACGTA 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 12, 823
Eco57I CTGAAG 1 cut(s) 671
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 8, 138
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 7 cut(s) 187, 298, 490, 835, 881, 938, 952
FaqI GGGAC 2 cut(s) 88, 205
FatI CATG 7 cut(s) 183, 294, 486, 831, 877, 934, 948
FbaI TGATCA 1 cut(s) 469
FokI GGATG 1 cut(s) 461
FspBI CTAG 3 cut(s) 257, 671, 854
HaeIII GGCC 1 cut(s) 349
HapII CCGG 2 cut(s) 166, 350
HgaI GACGC 1 cut(s) 947
Hin1I GRCGYC 1 cut(s) 939
Hin1II CATG 7 cut(s) 187, 298, 490, 835, 881, 938, 952
HincII GTYRAC 1 cut(s) 341
HindII GTYRAC 1 cut(s) 341
HinfI GANTC 3 cut(s) 290, 593, 729
HpaII CCGG 2 cut(s) 166, 350
HphI GGTGA 1 cut(s) 689
Hpy166II GTNNAC 2 cut(s) 341, 891
Hpy188I TCNGA 1 cut(s) 767
Hpy188III TCNNGA 5 cut(s) 119, 245, 371, 590, 878
Hpy8I GTNNAC 2 cut(s) 341, 891
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 4 cut(s) 158, 347, 663, 733
HpyCH4III ACNGT 2 cut(s) 544, 895
HpyCH4IV ACGT 5 cut(s) 105, 270, 330, 337, 450
HpyCH4V TGCA 2 cut(s) 724, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 841
HpyF3I CTNAG 2 cut(s) 91, 243
HpySE526I ACGT 5 cut(s) 105, 270, 330, 337, 450
Hsp92I GRCGYC 1 cut(s) 939
Hsp92II CATG 7 cut(s) 187, 298, 490, 835, 881, 938, 952
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 382, 469, 643
LmnI GCTCC 1 cut(s) 169
MaeI CTAG 3 cut(s) 257, 671, 854
MaeII ACGT 5 cut(s) 105, 270, 330, 337, 450
MaeIII GTNAC 4 cut(s) 266, 331, 421, 698
MalI GATC 3 cut(s) 384, 471, 645
MboI GATC 3 cut(s) 382, 469, 643
MboII GAAGA 2 cut(s) 134, 857
MluCI AATT 5 cut(s) 30, 225, 236, 407, 770
MlyI GAGTC 1 cut(s) 738
MmeI TCCRAC 1 cut(s) 949
MnlI CCTC 6 cut(s) 49, 252, 256, 264, 657, 814
MroXI GAANNNNTTC 2 cut(s) 543, 864
MseI TTAA 3 cut(s) 174, 228, 410
MslI CAYNNNNRTG 1 cut(s) 947
MspI CCGG 2 cut(s) 166, 350
MspR9I CCNGG 4 cut(s) 10, 140, 166, 167
MvaI CCWGG 2 cut(s) 10, 140
MwoI GCNNNNNNNGC 1 cut(s) 841
NciI CCSGG 2 cut(s) 166, 167
NdeII GATC 3 cut(s) 382, 469, 643
NlaIII CATG 7 cut(s) 187, 298, 490, 835, 881, 938, 952
NlaIV GGNNCC 1 cut(s) 14
NmuCI GTSAC 2 cut(s) 331, 421
PagI TCATGA 1 cut(s) 877
PaqCI CACCTGC 1 cut(s) 665
PdmI GAANNNNTTC 2 cut(s) 543, 864
PfeI GAWTC 2 cut(s) 290, 593
PfoI TCCNGGA 1 cut(s) 8
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
Ppu21I YACGTR 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 12
PsiI TTATAA 1 cut(s) 506
Psp5II RGGWCCY 1 cut(s) 12
Psp6I CCWGG 2 cut(s) 8, 138
PspGI CCWGG 2 cut(s) 8, 138
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 2 cut(s) 12, 823
PspPPI RGGWCCY 1 cut(s) 12
RsaI GTAC 2 cut(s) 96, 801
RsaNI GTAC 2 cut(s) 95, 800
RseI CAYNNNNRTG 1 cut(s) 947
SaqAI TTAA 3 cut(s) 174, 228, 410
Sau3AI GATC 3 cut(s) 382, 469, 643
Sau96I GGNCC 2 cut(s) 12, 823
SchI GAGTC 1 cut(s) 738
ScrFI CCNGG 4 cut(s) 10, 140, 166, 167
SinI GGWCC 2 cut(s) 12, 823
SmaI CCCGGG 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 947
SnaBI TACGTA 1 cut(s) 271
Sse9I AATT 5 cut(s) 30, 225, 236, 407, 770
SspI AATATT 1 cut(s) 872
SspMI CTAG 3 cut(s) 257, 671, 854
StyD4I CCNGG 4 cut(s) 8, 138, 164, 165
TaaI ACNGT 2 cut(s) 544, 895
TaiI ACGT 5 cut(s) 108, 273, 333, 340, 453
TaqI TCGA 2 cut(s) 120, 482
TaqII GACCGA 1 cut(s) 901
TasI AATT 5 cut(s) 30, 225, 236, 407, 770
TfiI GAWTC 2 cut(s) 290, 593
Tru1I TTAA 3 cut(s) 174, 228, 410
Tru9I TTAA 3 cut(s) 174, 228, 410
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 2 cut(s) 331, 421
Tsp45I GTSAC 2 cut(s) 331, 421
TspDTI ATGAA 4 cut(s) 531, 546, 645, 894
TspGWI ACGGA 1 cut(s) 830
TspMI CCCGGG 1 cut(s) 165
TspRI CASTG 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 12, 823
XapI RAATTY 1 cut(s) 30
XmaI CCCGGG 1 cut(s) 165
XmnI GAANNNNTTC 2 cut(s) 543, 864
XspI CTAG 3 cut(s) 257, 671, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.