MD08G1249100.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0021152
Physical Location & Seq
Reverse (-)
31349994 .. 31350611
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1249100.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCATGTGAAGAAGAATAATGCGATGATGGTGCTGCAGCCAGTACTACTTGTGATGGTGATGTTAGGCCTCACTGCCCCCACCCAAACCAACGCCTTGATTCAGTTCAACCCAATATCCTCCTCCCTCTGGGACCACCACATGATCGATGACCCTTTCAGAATTCTCGAACAGACTCCCTTCACCATCCCAAACCCTACCGTTCAAGAGGCGCTAGCTCTGGCACGTGCCGACTGGAAAGAGACGGCGACTGCACACGTGATCTCATTGGACGTCCCGGGGATGAAGAAGGAGGGCGTGAAGATAGAGGTGGAGGAGAACAGGGTGCTGAGGATCAGCGGAGAGAGGAAGGCCGACGACCAGGAAGGAGATCAGGGCGACAACTACAAGTGGCACCGTGCTGAGAGGACAAATGGCAAGTTCTGGAGGCAGTTCAGGCTGCCTGGGAACGCCGACTTGGATCAGATCAAGGCTCATCTCGAGGACGGGGTGCTCAGGATTACGGTGCCAAAGTTTGCGGCGGAGAAGAAGAGGCAGCCCAAGCTCATCAGCATTGCCTCGTCTTCTTCCGCGTCTGCTGATCATGGGGTGGATGTCAACCCTACAAAGGTCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

206

Amino Acids

22.93

Weight (kDa)

7.08

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 78 - 185 8.8e-29 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021152)

Species Orthologous Gene IDs
malus_domestica MD08G1249100.v1.1 MD15G1443700.v1.1
prunus_persica Prupe.1G586200_v2.0.a1
pyrus_communis pycom08g21560 pycom15g39120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 276
AccB1I GGYRCC 2 cut(s) 393, 505
AccII CGCG 1 cut(s) 572
AciI CCGC 4 cut(s) 339, 518, 521, 570
AclWI GGATC 2 cut(s) 341, 468
AcsI RAATTY 1 cut(s) 162
AcvI CACGTG 2 cut(s) 227, 259
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 2 cut(s) 129, 607
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 2 cut(s) 109, 206
AjnI CCWGG 2 cut(s) 360, 442
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 2 cut(s) 218, 544
AluI AGCT 2 cut(s) 218, 544
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 2 cut(s) 341, 468
Ama87I CYCGRG 2 cut(s) 277, 479
AoxI GGCC 2 cut(s) 67, 351
ApeKI GCWGC 4 cut(s) 34, 37, 439, 535
ApoI RAATTY 1 cut(s) 162
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 2 cut(s) 278, 279
AsuHPI GGTGA 2 cut(s) 70, 175
AsuNHI GCTAGC 1 cut(s) 214
AvaI CYCGRG 2 cut(s) 277, 479
AvaII GGWCC 1 cut(s) 133
BanI GGYRCC 2 cut(s) 393, 505
BbrPI CACGTG 2 cut(s) 227, 259
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 1 cut(s) 495
BbvCI CCTCAGC 1 cut(s) 329
BbvI GCAGC 4 cut(s) 21, 49, 426, 547
BccI CCATC 3 cut(s) 22, 49, 194
BceAI ACGGC 1 cut(s) 261
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 2 cut(s) 362, 444
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 2 cut(s) 278, 279
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 215
BisI GCNGC 5 cut(s) 35, 38, 440, 519, 536
BlsI GCNGC 5 cut(s) 36, 39, 441, 520, 537
BmcAI AGTACT 1 cut(s) 45
Bme1390I CCNGG 4 cut(s) 278, 279, 362, 444
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 277, 479
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 3 cut(s) 134, 395, 507
BmrFI CCNGG 4 cut(s) 278, 279, 362, 444
BmtI GCTAGC 1 cut(s) 218
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 445
Bpu10I CCTNAGC 2 cut(s) 329, 494
BpuMI CCSGG 2 cut(s) 278, 279
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 2 cut(s) 227, 259
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 277, 278, 443
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 607
Bse1I ACTGG 2 cut(s) 41, 239
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 2 cut(s) 362, 444
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 3 cut(s) 277, 278, 443
BseGI GGATG 3 cut(s) 186, 288, 598
BseLI CCNNNNNNNGG 2 cut(s) 129, 607
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 3 cut(s) 320, 393, 508
BseNI ACTGG 2 cut(s) 41, 239
BseRI GAGGAG 2 cut(s) 112, 329
BseXI GCAGC 4 cut(s) 21, 49, 426, 547
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 572
BshFI GGCC 2 cut(s) 69, 353
BshNI GGYRCC 2 cut(s) 393, 505
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 495
BsiHKCI CYCGRG 2 cut(s) 277, 479
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 2 cut(s) 146, 260
BslI CCNNNNNNNGG 2 cut(s) 129, 607
BsmAI GTCTC 1 cut(s) 236
BsmBI CGTCTC 1 cut(s) 236
BsmFI GGGAC 2 cut(s) 146, 260
BsnI GGCC 2 cut(s) 69, 353
BsoBI CYCGRG 2 cut(s) 277, 479
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
BspACI CCGC 4 cut(s) 339, 518, 521, 570
BspANI GGCC 2 cut(s) 69, 353
BspCNI CTCAG 3 cut(s) 321, 394, 507
BspDI ATCGAT 1 cut(s) 147
BspFNI CGCG 1 cut(s) 572
BspLI GGNNCC 3 cut(s) 134, 395, 507
BspMAI CTGCAG 1 cut(s) 39
BspOI GCTAGC 1 cut(s) 218
BspPI GGATC 2 cut(s) 341, 468
BspT107I GGYRCC 2 cut(s) 393, 505
BsrDI GCAATG 1 cut(s) 552
BsrI ACTGG 2 cut(s) 41, 239
BssECI CCNNGG 3 cut(s) 277, 278, 443
BssMI GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
BssNI GRCGYC 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 362, 444
Bst4CI ACNGT 3 cut(s) 202, 398, 505
Bst6I CTCTTC 1 cut(s) 524
BstACI GRCGYC 1 cut(s) 273
BstBAI YACGTR 2 cut(s) 227, 259
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 3 cut(s) 329, 402, 494
BstF5I GGATG 3 cut(s) 186, 288, 598
BstFNI CGCG 1 cut(s) 572
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 7 cut(s) 147, 264, 336, 373, 463, 468, 583
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
BstMWI GCNNNNNNNGC 2 cut(s) 436, 541
BstNI CCWGG 2 cut(s) 362, 444
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 4 cut(s) 276, 277, 360, 442
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 4 cut(s) 21, 49, 426, 547
BstV2I GAAGAC 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 147
BsuRI GGCC 2 cut(s) 69, 353
BsuTUI ATCGAT 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 186, 288, 598
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 133
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 1 cut(s) 561
Csp6I GTAC 1 cut(s) 44
CviAII CATG 4 cut(s) 5, 142, 584, 615
CviJI RGCY 8 cut(s) 40, 69, 218, 353, 439, 473, 538, 544
CviKI_1 RGCY 8 cut(s) 40, 69, 218, 353, 439, 473, 538, 544
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 3 cut(s) 329, 402, 494
DpnI GATC 7 cut(s) 146, 263, 335, 372, 462, 467, 582
DpnII GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
EciI GGCGGA 1 cut(s) 536
Eco147I AGGCCT 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 133
Eco72I CACGTG 2 cut(s) 227, 259
Eco88I CYCGRG 2 cut(s) 277, 479
EcoRI GAATTC 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 360, 442
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 4 cut(s) 8, 145, 587, 618
FaiI YATR 4 cut(s) 6, 143, 585, 616
FaqI GGGAC 2 cut(s) 146, 260
FatI CATG 4 cut(s) 4, 141, 583, 614
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 5 cut(s) 35, 38, 440, 519, 536
FokI GGATG 3 cut(s) 173, 295, 605
Fsp4HI GCNGC 5 cut(s) 35, 38, 440, 519, 536
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 213
GluI GCNGC 5 cut(s) 35, 38, 440, 519, 536
GsuI CTGGAG 1 cut(s) 445
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 2 cut(s) 69, 353
HapII CCGG 1 cut(s) 278
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 4 cut(s) 8, 145, 587, 618
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 2 cut(s) 100, 175
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 70, 175
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 2 cut(s) 161, 465
Hpy188III TCNNGA 5 cut(s) 167, 206, 424, 479, 496
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 4 cut(s) 190, 283, 343, 359
HpyCH4III ACNGT 3 cut(s) 202, 398, 505
HpyCH4IV ACGT 3 cut(s) 226, 258, 273
HpyCH4V TGCA 3 cut(s) 4, 37, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 436, 541
HpyF3I CTNAG 3 cut(s) 329, 402, 494
HpySE526I ACGT 3 cut(s) 226, 258, 273
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 4 cut(s) 8, 145, 587, 618
HspAI GCGC 1 cut(s) 212
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
Lsp1109I GCAGC 4 cut(s) 21, 49, 426, 547
MaeI CTAG 1 cut(s) 215
MaeII ACGT 3 cut(s) 226, 258, 273
MalI GATC 7 cut(s) 146, 263, 335, 372, 462, 467, 582
MboI GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
MboII GAAGA 8 cut(s) 22, 25, 298, 313, 538, 541, 555, 558
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 1 cut(s) 162
MlyI GAGTC 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 4 cut(s) 278, 279, 362, 444
MvaI CCWGG 2 cut(s) 362, 444
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 2 cut(s) 436, 541
NciI CCSGG 2 cut(s) 278, 279
NdeII GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
NheI GCTAGC 1 cut(s) 214
NlaIII CATG 4 cut(s) 8, 145, 587, 618
NlaIV GGNNCC 3 cut(s) 134, 395, 507
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 479
PceI AGGCCT 1 cut(s) 69
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 5 cut(s) 36, 39, 441, 520, 537
PleI GAGTC 1 cut(s) 169
PmaCI CACGTG 2 cut(s) 227, 259
PmlI CACGTG 2 cut(s) 227, 259
PpsI GAGTC 1 cut(s) 169
Ppu21I YACGTR 2 cut(s) 227, 259
Psp6I CCWGG 2 cut(s) 360, 442
PspCI CACGTG 2 cut(s) 227, 259
PspGI CCWGG 2 cut(s) 360, 442
PspN4I GGNNCC 3 cut(s) 134, 395, 507
PspPI GGNCC 1 cut(s) 133
PstI CTGCAG 1 cut(s) 39
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SatI GCNGC 5 cut(s) 35, 38, 440, 519, 536
Sau3AI GATC 7 cut(s) 144, 261, 333, 370, 460, 465, 580
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 45
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 4 cut(s) 278, 279, 362, 444
SduI GDGCHC 1 cut(s) 495
SetI ASST 7 cut(s) 220, 229, 261, 276, 312, 546, 612
SfcI CTRYAG 1 cut(s) 35
Sfr274I CTCGAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 479
SmaI CCCGGG 1 cut(s) 279
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 1 cut(s) 162
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 4 cut(s) 339, 518, 521, 570
SspMI CTAG 1 cut(s) 215
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 276, 277, 360, 442
TaaI ACNGT 3 cut(s) 202, 398, 505
TaiI ACGT 3 cut(s) 229, 261, 276
TaqI TCGA 3 cut(s) 147, 168, 480
TasI AATT 1 cut(s) 162
TatI WGTACW 1 cut(s) 43
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 1 cut(s) 100
TscAI CASTG 1 cut(s) 79
TseI GCWGC 4 cut(s) 34, 37, 439, 535
TspDTI ATGAA 1 cut(s) 299
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 133
XapI RAATTY 1 cut(s) 162
XceI RCATGY 1 cut(s) 8
XhoI CTCGAG 1 cut(s) 479
XmaI CCCGGG 1 cut(s) 277
XspI CTAG 1 cut(s) 215
ZraI GACGTC 1 cut(s) 274
ZrmI AGTACT 1 cut(s) 45
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.