pycom08g21560

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0021152
Physical Location & Seq
Reverse (-)
21021891 .. 21022514
624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21560.1

Sequence Viewer

Length: 624 bp
ATGCATGTGAAGAAGAATAATGCGATGATGGTGCTGCAGCCAGTACTACTTGTGATGATGATGTTAGGCCTCACTGCCCCCACCCAAACCAACGCCTTGATTCAGTTCAACCCAATATCCTCCTCCCCCTGGGACCACCACATGATCAATGATCCCTTCAGAATTCTCGAACAGACTCCCTTCACCGTCCCAAACCCTACTAAAGCCGTTCAAGAGGCGCTCGCTCTGGCACGTGCCGACTGGAAGGAGACGGCGACTGCGCACGTGATCTCATTGGACGTCCCCGGCCTGAAGAAGGAGGACGTGAAGATAGAGGTGGAGGAGAACAGGCTGCTGAGGATCAGCGGAGAGAGGAAGGCCGACGATCAGGAAGGGGATCAGGGCGACAATTACAAGTGGCACCGCGCTGAGAGGACAAATGGCAAGTTCTGGAGGCAATTCAGGCTGCCTGGGAACACCGACTTGGATCAGATCAAGGCTCATCTCGAGGACGGGGTGCTCAGGATTACGGTGCCAAAGTTTGCGGCGGAGAAGAAGAGGCAGCCCAAGCTTATCAGCATTGCCTCTTCTTCTTCCACGTCAGCTGCTCATGGGTCAGATGTCAACCCTACAAAGGTCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

208

Amino Acids

23.19

Weight (kDa)

8.8

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 80 - 187 1.6e-29 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021152)

Species Orthologous Gene IDs
malus_domestica MD08G1249100.v1.1 MD15G1443700.v1.1
prunus_persica Prupe.1G586200_v2.0.a1
pyrus_communis pycom08g21560 pycom15g39120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
Acc16I TGCGCA 1 cut(s) 261
AccB1I GGYRCC 2 cut(s) 399, 511
AccII CGCG 1 cut(s) 405
AciI CCGC 4 cut(s) 345, 403, 524, 527
AclWI GGATC 4 cut(s) 146, 347, 384, 474
AcsI RAATTY 1 cut(s) 162
AcuI CTGAAG 2 cut(s) 142, 311
AcvI CACGTG 2 cut(s) 233, 265
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 3 cut(s) 129, 295, 613
AgsI TTSAA 2 cut(s) 109, 212
AjiI CACGTC 2 cut(s) 304, 579
AjnI CCWGG 2 cut(s) 128, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AluBI AGCT 2 cut(s) 550, 584
AluI AGCT 2 cut(s) 550, 584
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 4 cut(s) 146, 347, 384, 474
Ama87I CYCGRG 1 cut(s) 485
AoxI GGCC 3 cut(s) 67, 286, 357
ApeKI GCWGC 6 cut(s) 34, 37, 331, 445, 541, 584
ApoI RAATTY 1 cut(s) 162
AspLEI GCGC 3 cut(s) 220, 262, 407
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 1 cut(s) 133
BanI GGYRCC 2 cut(s) 399, 511
BbrPI CACGTG 2 cut(s) 233, 265
Bbv12I GWGCWC 1 cut(s) 501
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 6 cut(s) 21, 49, 318, 432, 553, 571
BccI CCATC 1 cut(s) 22
BceAI ACGGC 2 cut(s) 191, 267
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 2 cut(s) 130, 450
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 7 cut(s) 35, 38, 332, 446, 525, 542, 585
BlsI GCNGC 7 cut(s) 36, 39, 333, 447, 526, 543, 586
BmcAI AGTACT 1 cut(s) 45
Bme1390I CCNGG 3 cut(s) 130, 285, 450
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 485
BmgBI CACGTC 2 cut(s) 304, 579
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 3 cut(s) 134, 401, 513
BmrFI CCNGG 3 cut(s) 130, 285, 450
BpmI CTGGAG 1 cut(s) 451
Bpu10I CCTNAGC 2 cut(s) 335, 500
BpuMI CCSGG 1 cut(s) 285
BsaAI YACGTR 2 cut(s) 233, 265
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 4 cut(s) 128, 129, 283, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 295, 613
Bse1I ACTGG 2 cut(s) 41, 245
Bse3DI GCAATG 1 cut(s) 558
BseBI CCWGG 2 cut(s) 130, 450
BseDI CCNNGG 4 cut(s) 128, 129, 283, 449
BseLI CCNNNNNNNGG 3 cut(s) 129, 295, 613
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 3 cut(s) 326, 399, 514
BseNI ACTGG 2 cut(s) 41, 245
BseRI GAGGAG 2 cut(s) 112, 335
BseXI GCAGC 6 cut(s) 21, 49, 318, 432, 553, 571
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 3 cut(s) 69, 288, 359
BshNI GGYRCC 2 cut(s) 399, 511
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 3 cut(s) 146, 173, 266
BslI CCNNNNNNNGG 3 cut(s) 129, 295, 613
BsmAI GTCTC 1 cut(s) 242
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 3 cut(s) 146, 173, 266
BsnI GGCC 3 cut(s) 69, 288, 359
BsoBI CYCGRG 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
BspACI CCGC 4 cut(s) 345, 403, 524, 527
BspANI GGCC 3 cut(s) 69, 288, 359
BspCNI CTCAG 3 cut(s) 327, 400, 513
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 134, 401, 513
BspMAI CTGCAG 1 cut(s) 39
BspPI GGATC 4 cut(s) 146, 347, 384, 474
BspT107I GGYRCC 2 cut(s) 399, 511
BsrDI GCAATG 1 cut(s) 558
BsrI ACTGG 2 cut(s) 41, 245
BssECI CCNNGG 4 cut(s) 128, 129, 283, 449
BssMI GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
BssNI GRCGYC 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 130, 450
Bst4CI ACNGT 2 cut(s) 187, 511
Bst6I CTCTTC 2 cut(s) 530, 571
BstACI GRCGYC 1 cut(s) 279
BstBAI YACGTR 2 cut(s) 233, 265
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 3 cut(s) 335, 408, 500
BstENI CCTNNNNNAGG 1 cut(s) 293
BstFNI CGCG 1 cut(s) 405
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 3 cut(s) 220, 262, 407
BstKTI GATC 8 cut(s) 147, 154, 270, 342, 367, 379, 469, 474
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
BstMWI GCNNNNNNNGC 2 cut(s) 442, 547
BstNI CCWGG 2 cut(s) 130, 450
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 3 cut(s) 128, 283, 448
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 6 cut(s) 21, 49, 318, 432, 553, 571
BsuRI GGCC 3 cut(s) 69, 288, 359
BtgZI GCGATG 1 cut(s) 38
BtrI CACGTC 2 cut(s) 304, 579
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 3 cut(s) 220, 262, 407
Cfr13I GGNCC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 44
CviAII CATG 4 cut(s) 5, 142, 590, 621
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 3 cut(s) 335, 408, 500
DpnI GATC 8 cut(s) 146, 153, 269, 341, 366, 378, 468, 473
DpnII GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
Eam1104I CTCTTC 2 cut(s) 530, 571
EarI CTCTTC 2 cut(s) 530, 571
EciI GGCGGA 1 cut(s) 542
Eco147I AGGCCT 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 142, 311
Eco72I CACGTG 2 cut(s) 233, 265
Eco88I CYCGRG 1 cut(s) 485
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 128, 448
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 4 cut(s) 8, 145, 593, 624
FaiI YATR 4 cut(s) 6, 143, 591, 622
FaqI GGGAC 3 cut(s) 146, 173, 266
FatI CATG 4 cut(s) 4, 141, 589, 620
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 7 cut(s) 35, 38, 332, 446, 525, 542, 585
Fsp4HI GCNGC 7 cut(s) 35, 38, 332, 446, 525, 542, 585
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 3 cut(s) 219, 261, 406
GluI GCNGC 7 cut(s) 35, 38, 332, 446, 525, 542, 585
GsuI CTGGAG 1 cut(s) 451
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 3 cut(s) 69, 288, 359
HapII CCGG 1 cut(s) 285
HhaI GCGC 3 cut(s) 220, 262, 407
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 4 cut(s) 8, 145, 593, 624
Hin6I GCGC 3 cut(s) 218, 260, 405
HinP1I GCGC 3 cut(s) 218, 260, 405
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 2 cut(s) 100, 175
HpaII CCGG 1 cut(s) 285
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 604
Hpy188I TCNGA 3 cut(s) 161, 471, 598
Hpy188III TCNNGA 6 cut(s) 167, 212, 368, 430, 485, 502
Hpy8I GTNNAC 1 cut(s) 604
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 6 cut(s) 166, 190, 238, 289, 349, 365
HpyCH4III ACNGT 2 cut(s) 187, 511
HpyCH4IV ACGT 5 cut(s) 232, 264, 279, 303, 578
HpyCH4V TGCA 2 cut(s) 4, 37
HpyF10VI GCNNNNNNNGC 2 cut(s) 442, 547
HpyF3I CTNAG 3 cut(s) 335, 408, 500
HpySE526I ACGT 5 cut(s) 232, 264, 279, 303, 578
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 4 cut(s) 8, 145, 593, 624
HspAI GCGC 3 cut(s) 218, 260, 405
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
Lsp1109I GCAGC 6 cut(s) 21, 49, 318, 432, 553, 571
MaeII ACGT 5 cut(s) 232, 264, 279, 303, 578
MalI GATC 8 cut(s) 146, 153, 269, 341, 366, 378, 468, 473
MboI GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
MboII GAAGA 9 cut(s) 22, 25, 304, 319, 544, 547, 558, 561, 564
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 3 cut(s) 162, 388, 437
MlyI GAGTC 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 2 cut(s) 345, 584
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 3 cut(s) 130, 285, 450
MvaI CCWGG 2 cut(s) 130, 450
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 2 cut(s) 442, 547
NciI CCSGG 1 cut(s) 285
NdeII GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
NlaIII CATG 4 cut(s) 8, 145, 593, 624
NlaIV GGNNCC 3 cut(s) 134, 401, 513
NsbI TGCGCA 1 cut(s) 261
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 485
PasI CCCWGGG 1 cut(s) 129
PceI AGGCCT 1 cut(s) 69
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 7 cut(s) 36, 39, 333, 447, 526, 543, 586
PleI GAGTC 1 cut(s) 169
PmaCI CACGTG 2 cut(s) 233, 265
PmlI CACGTG 2 cut(s) 233, 265
PpsI GAGTC 1 cut(s) 169
Ppu21I YACGTR 2 cut(s) 233, 265
Psp6I CCWGG 2 cut(s) 128, 448
PspCI CACGTG 2 cut(s) 233, 265
PspGI CCWGG 2 cut(s) 128, 448
PspN4I GGNNCC 3 cut(s) 134, 401, 513
PspPI GGNCC 1 cut(s) 133
PstI CTGCAG 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SatI GCNGC 7 cut(s) 35, 38, 332, 446, 525, 542, 585
Sau3AI GATC 8 cut(s) 144, 151, 267, 339, 364, 376, 466, 471
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 45
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 3 cut(s) 130, 285, 450
SduI GDGCHC 1 cut(s) 501
SetI ASST 9 cut(s) 235, 267, 282, 306, 318, 552, 581, 586, 618
SfcI CTRYAG 1 cut(s) 35
Sfr274I CTCGAG 1 cut(s) 485
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 3 cut(s) 162, 388, 437
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 4 cut(s) 345, 403, 524, 527
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 3 cut(s) 128, 283, 448
TaaI ACNGT 2 cut(s) 187, 511
TaiI ACGT 5 cut(s) 235, 267, 282, 306, 581
TaqI TCGA 2 cut(s) 168, 486
TasI AATT 3 cut(s) 162, 388, 437
TatI WGTACW 1 cut(s) 43
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 1 cut(s) 100
TscAI CASTG 1 cut(s) 79
TseI GCWGC 6 cut(s) 34, 37, 331, 445, 541, 584
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 1 cut(s) 162
XceI RCATGY 1 cut(s) 8
XhoI CTCGAG 1 cut(s) 485
ZraI GACGTC 1 cut(s) 280
ZrmI AGTACT 1 cut(s) 45
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.