MD15G1443700.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0021152
Physical Location & Seq
Reverse (-)
54408605 .. 54409228
624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1443700.v1.1.491

Sequence Viewer

Length: 624 bp
ATGCATATGAAGAATAACGTGATGATGGTGGTGCAGTCAGTACTACTTGTGATGATGATGTTAGGCCTCATGGCCCCCTCCCAAACCAATGCCTTGATTCCGTACAACCCAATATCCCCCTCCTCCCTCTTGGACCACATGATGATCGATGACCCCTTCAGAACTCTCGAACAGACTCCCTTCACCGTCCCAAACCCTACTAAAGTCGCTCAAGAGGCGCTCGCTCTGGCACGTGCTGACTGGAAAGAGACGGCGACTGCACACGTGATCTCATTGGACGTCCCGGGGCTGAAGAGGGAGGACGTCAAGATAGAGGTGGAGGAGAACAGGGTGCTGAGGATCAGCGGAGAGAGGAAGGCCGACGACCGGGAAGGTGAACTGCAGGGCGACAACTACAAGTGGCACCGCGCTGAGAGGACAGACGGCAAGTTCTGGAGGCAGTTCAGACTGCCTGGGAACGCCGACTTGGATCAGATAAAGGCTCACCTGGAGGACGGGGTGCTGAAGATTACGGTGCCAAAGTTAGCGGCGGAGAAGAAGAGGCAGCCCAAGCTCATCAACATCGCCTCTTCTTCCTCGTCTGCTGATCATGGGGCGGATGTCGAACCTACAAAGGTCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

208

Amino Acids

23.17

Weight (kDa)

6.31

Isoelectric Point (pI)

39.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 80 - 188 3.4e-29 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021152)

Species Orthologous Gene IDs
malus_domestica MD08G1249100.v1.1 MD15G1443700.v1.1
prunus_persica Prupe.1G586200_v2.0.a1
pyrus_communis pycom08g21560 pycom15g39120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 282, 306
AccB1I GGYRCC 2 cut(s) 402, 514
AccII CGCG 1 cut(s) 408
AciI CCGC 5 cut(s) 345, 406, 527, 530, 596
AclWI GGATC 2 cut(s) 347, 477
AcuI CTGAAG 3 cut(s) 142, 311, 524
AcvI CACGTG 2 cut(s) 233, 265
AcyI GRCGYC 2 cut(s) 279, 303
AfaI GTAC 2 cut(s) 42, 104
AfiI CCNNNNNNNGG 1 cut(s) 366
AflIII ACRYGT 1 cut(s) 262
AjnI CCWGG 2 cut(s) 451, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AluBI AGCT 1 cut(s) 553
AluI AGCT 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 2 cut(s) 347, 477
Ama87I CYCGRG 1 cut(s) 283
AoxI GGCC 3 cut(s) 64, 72, 357
ApeKI GCWGC 1 cut(s) 544
AspLEI GCGC 2 cut(s) 220, 410
AspS9I GGNCC 2 cut(s) 73, 133
AsuC2I CCSGG 3 cut(s) 284, 285, 368
AsuHPI GGTGA 3 cut(s) 175, 386, 476
AvaI CYCGRG 1 cut(s) 283
AvaII GGWCC 1 cut(s) 133
BanI GGYRCC 2 cut(s) 402, 514
BbrPI CACGTG 2 cut(s) 233, 265
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 1 cut(s) 556
BccI CCATC 1 cut(s) 19
BceAI ACGGC 2 cut(s) 267, 439
BciT130I CCWGG 2 cut(s) 453, 488
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 3 cut(s) 284, 285, 368
BcoDI GTCTC 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 2 cut(s) 528, 545
BlsI GCNGC 2 cut(s) 529, 546
BmcAI AGTACT 1 cut(s) 42
Bme1390I CCNGG 5 cut(s) 284, 285, 368, 453, 488
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 73, 133
BmiI GGNNCC 3 cut(s) 75, 404, 516
BmrFI CCNGG 5 cut(s) 284, 285, 368, 453, 488
BpmI CTGGAG 2 cut(s) 454, 509
Bpu10I CCTNAGC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 195
BpuMI CCSGG 3 cut(s) 284, 285, 368
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 2 cut(s) 233, 265
BsaHI GRCGYC 2 cut(s) 279, 303
BsaJI CCNNGG 3 cut(s) 283, 284, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 245
BseBI CCWGG 2 cut(s) 453, 488
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 3 cut(s) 283, 284, 452
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 326, 402
BseNI ACTGG 1 cut(s) 245
BseRI GAGGAG 2 cut(s) 112, 335
BseXI GCAGC 1 cut(s) 556
BsgI GTGCAG 2 cut(s) 53, 243
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 3 cut(s) 66, 74, 359
BshNI GGYRCC 2 cut(s) 402, 514
BshVI ATCGAT 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 283
BsiSI CCGG 2 cut(s) 284, 367
BslFI GGGAC 2 cut(s) 173, 266
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 242
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 2 cut(s) 173, 266
BsnI GGCC 3 cut(s) 66, 74, 359
BsoBI CYCGRG 1 cut(s) 283
Bsp143I GATC 5 cut(s) 144, 267, 339, 469, 586
BspACI CCGC 5 cut(s) 345, 406, 527, 530, 596
BspANI GGCC 3 cut(s) 66, 74, 359
BspCNI CTCAG 2 cut(s) 327, 403
BspDI ATCGAT 1 cut(s) 147
BspFNI CGCG 1 cut(s) 408
BspLI GGNNCC 3 cut(s) 75, 404, 516
BspMAI CTGCAG 1 cut(s) 384
BspPI GGATC 2 cut(s) 347, 477
BspT107I GGYRCC 2 cut(s) 402, 514
BsrI ACTGG 1 cut(s) 245
BssECI CCNNGG 3 cut(s) 283, 284, 452
BssMI GATC 5 cut(s) 144, 267, 339, 469, 586
BssNI GRCGYC 2 cut(s) 279, 303
Bst2UI CCWGG 2 cut(s) 453, 488
Bst4CI ACNGT 2 cut(s) 187, 514
Bst6I CTCTTC 3 cut(s) 287, 533, 574
BstACI GRCGYC 2 cut(s) 279, 303
BstBAI YACGTR 2 cut(s) 233, 265
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 2 cut(s) 335, 411
BstF5I GGATG 1 cut(s) 604
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 220, 410
BstKTI GATC 5 cut(s) 147, 270, 342, 472, 589
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 5 cut(s) 144, 267, 339, 469, 586
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 2 cut(s) 215, 550
BstNI CCWGG 2 cut(s) 453, 488
BstSCI CCNGG 5 cut(s) 282, 283, 366, 451, 486
BstSFI CTRYAG 1 cut(s) 380
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 556
Bsu15I ATCGAT 1 cut(s) 147
BsuRI GGCC 3 cut(s) 66, 74, 359
BsuTUI ATCGAT 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 547
BtsCI GGATG 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 2 cut(s) 220, 410
Cfr13I GGNCC 2 cut(s) 73, 133
Cfr9I CCCGGG 1 cut(s) 283
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 2 cut(s) 41, 103
CviAII CATG 4 cut(s) 70, 139, 590, 621
CviJI RGCY 7 cut(s) 66, 74, 289, 359, 482, 547, 553
CviKI_1 RGCY 7 cut(s) 66, 74, 289, 359, 482, 547, 553
CviQI GTAC 2 cut(s) 41, 103
DdeI CTNAG 2 cut(s) 335, 411
DpnI GATC 5 cut(s) 146, 269, 341, 471, 588
DpnII GATC 5 cut(s) 144, 267, 339, 469, 586
Eam1104I CTCTTC 3 cut(s) 287, 533, 574
EarI CTCTTC 3 cut(s) 287, 533, 574
EciI GGCGGA 2 cut(s) 545, 611
Eco147I AGGCCT 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 133
Eco57I CTGAAG 3 cut(s) 142, 311, 524
Eco72I CACGTG 2 cut(s) 233, 265
Eco88I CYCGRG 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 451, 486
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 4 cut(s) 73, 142, 593, 624
FaiI YATR 6 cut(s) 6, 8, 71, 140, 591, 622
FaqI GGGAC 2 cut(s) 173, 266
FatI CATG 4 cut(s) 69, 138, 589, 620
FauNDI CATATG 1 cut(s) 6
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 2 cut(s) 528, 545
FokI GGATG 1 cut(s) 611
Fsp4HI GCNGC 2 cut(s) 528, 545
GlaI GCGC 2 cut(s) 219, 409
GluI GCNGC 2 cut(s) 528, 545
GsuI CTGGAG 2 cut(s) 454, 509
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 3 cut(s) 66, 74, 359
HapII CCGG 2 cut(s) 284, 367
HhaI GCGC 2 cut(s) 220, 410
Hin1I GRCGYC 2 cut(s) 279, 303
Hin1II CATG 4 cut(s) 73, 142, 593, 624
Hin6I GCGC 2 cut(s) 218, 408
HinP1I GCGC 2 cut(s) 218, 408
HinfI GANTC 2 cut(s) 97, 175
HpaII CCGG 2 cut(s) 284, 367
HphI GGTGA 3 cut(s) 175, 386, 476
Hpy166II GTNNAC 1 cut(s) 377
Hpy188I TCNGA 3 cut(s) 161, 446, 474
Hpy188III TCNNGA 4 cut(s) 167, 212, 307, 433
Hpy8I GTNNAC 1 cut(s) 377
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 4 cut(s) 166, 190, 349, 365
HpyCH4III ACNGT 2 cut(s) 187, 514
HpyCH4IV ACGT 5 cut(s) 18, 232, 264, 279, 303
HpyCH4V TGCA 4 cut(s) 4, 34, 260, 382
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 550
HpyF3I CTNAG 2 cut(s) 335, 411
HpySE526I ACGT 5 cut(s) 18, 232, 264, 279, 303
Hsp92I GRCGYC 2 cut(s) 279, 303
Hsp92II CATG 4 cut(s) 73, 142, 593, 624
HspAI GCGC 2 cut(s) 218, 408
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 5 cut(s) 144, 267, 339, 469, 586
Lsp1109I GCAGC 1 cut(s) 556
MaeII ACGT 5 cut(s) 18, 232, 264, 279, 303
MalI GATC 5 cut(s) 146, 269, 341, 471, 588
MboI GATC 5 cut(s) 144, 267, 339, 469, 586
MboII GAAGA 7 cut(s) 22, 304, 517, 547, 550, 561, 564
MlyI GAGTC 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 2 cut(s) 284, 367
MspR9I CCNGG 5 cut(s) 284, 285, 368, 453, 488
MvaI CCWGG 2 cut(s) 453, 488
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 2 cut(s) 215, 550
NciI CCSGG 3 cut(s) 284, 285, 368
NdeI CATATG 1 cut(s) 6
NdeII GATC 5 cut(s) 144, 267, 339, 469, 586
NlaIII CATG 4 cut(s) 73, 142, 593, 624
NlaIV GGNNCC 3 cut(s) 75, 404, 516
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 66
PfeI GAWTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 529, 546
PleI GAGTC 1 cut(s) 169
PmaCI CACGTG 2 cut(s) 233, 265
PmlI CACGTG 2 cut(s) 233, 265
PpsI GAGTC 1 cut(s) 169
Ppu21I YACGTR 2 cut(s) 233, 265
Psp6I CCWGG 2 cut(s) 451, 486
PspCI CACGTG 2 cut(s) 233, 265
PspGI CCWGG 2 cut(s) 451, 486
PspN4I GGNNCC 3 cut(s) 75, 404, 516
PspPI GGNCC 2 cut(s) 73, 133
PstI CTGCAG 1 cut(s) 384
RsaI GTAC 2 cut(s) 42, 104
RsaNI GTAC 2 cut(s) 41, 103
SatI GCNGC 2 cut(s) 528, 545
Sau3AI GATC 5 cut(s) 144, 267, 339, 469, 586
Sau96I GGNCC 2 cut(s) 73, 133
ScaI AGTACT 1 cut(s) 42
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 5 cut(s) 284, 285, 368, 453, 488
SfcI CTRYAG 1 cut(s) 380
SinI GGWCC 1 cut(s) 133
SmaI CCCGGG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 5 cut(s) 345, 406, 527, 530, 596
StuI AGGCCT 1 cut(s) 66
StyD4I CCNGG 5 cut(s) 282, 283, 366, 451, 486
TaaI ACNGT 2 cut(s) 187, 514
TaiI ACGT 5 cut(s) 21, 235, 267, 282, 306
TaqI TCGA 3 cut(s) 147, 168, 603
TatI WGTACW 1 cut(s) 40
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 1 cut(s) 97
TseI GCWGC 1 cut(s) 544
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 90
TspMI CCCGGG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 133
XmaI CCCGGG 1 cut(s) 283
ZraI GACGTC 2 cut(s) 280, 304
ZrmI AGTACT 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.