MD08G1250700.v1.1
HSP70 Family

heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0022395
Physical Location & Seq
Reverse (-)
31567192 .. 31567422
231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1250700.v1.1.491

Sequence Viewer

Length: 231 bp
ATGGTGCTGACAAAGATGAAGGAGATCGCGGAGTCGTATTTGGGTCAGATGGTGAAGAACATCGTCCTGACTGTCCCTGCCTACTTCAACGACTCTCAGCAGCAAGCCACCAAGGACGCTGGTGCGATTTCCGGGCTCAATGTCATGCGGATCATCAACGAGCCAATGGCCGCCGCCATCGCCTACGGGCTTGATAAGAAGGCGTCGAGAAAGGGGAGCAGAACGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

77

Amino Acids

8.24

Weight (kDa)

9.63

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 1 - 68 2.8e-34 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022395)

Species Orthologous Gene IDs
malus_domestica MD08G1250700.v1.1
rosa_chinensis RchiOBHm_Chr1g0366771
rosa_laevigata RLG00000027323
rosa_samantha Rh6BG022800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 29
AciI CCGC 4 cut(s) 29, 148, 171, 174
AclWI GGATC 1 cut(s) 158
AcoI YGGCCR 1 cut(s) 168
AcyI GRCGYC 1 cut(s) 203
AgsI TTSAA 1 cut(s) 88
AlwI GGATC 1 cut(s) 158
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 1 cut(s) 100
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 64
BanII GRGCYC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 112
BccI CCATC 2 cut(s) 43, 185
BcnI CCSGG 1 cut(s) 133
BisI GCNGC 3 cut(s) 101, 171, 174
BlsI GCNGC 3 cut(s) 102, 172, 175
Bme1390I CCNGG 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 133
BpuMI CCSGG 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 170
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 59
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 24, 150
BspACI CCGC 4 cut(s) 29, 148, 171, 174
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 1 cut(s) 29
BspPI GGATC 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 2 cut(s) 24, 150
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 29
BstKTI GATC 2 cut(s) 27, 153
BstMBI GATC 2 cut(s) 24, 150
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 131
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 112
BsuRI GGCC 1 cut(s) 170
BtgZI GCGATG 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 105
CseI GACGC 2 cut(s) 125, 192
CviAII CATG 1 cut(s) 145
CviJI RGCY 5 cut(s) 107, 136, 163, 170, 190
CviKI_1 RGCY 5 cut(s) 107, 136, 163, 170, 190
DdeI CTNAG 1 cut(s) 96
DpnI GATC 2 cut(s) 26, 152
DpnII GATC 2 cut(s) 24, 150
EaeI YGGCCR 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 1 cut(s) 148
FaiI YATR 1 cut(s) 146
FaqI GGGAC 1 cut(s) 59
FatI CATG 1 cut(s) 144
Fnu4HI GCNGC 3 cut(s) 101, 171, 174
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 3 cut(s) 101, 171, 174
GluI GCNGC 3 cut(s) 101, 171, 174
HaeIII GGCC 1 cut(s) 170
HapII CCGG 1 cut(s) 132
HgaI GACGC 2 cut(s) 125, 192
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 1 cut(s) 148
HinfI GANTC 2 cut(s) 32, 92
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 3 cut(s) 67, 207, 228
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 13, 193
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 224
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 1 cut(s) 148
Kzo9I GATC 2 cut(s) 24, 150
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 4 cut(s) 80, 90, 105, 145
Lsp1109I GCAGC 1 cut(s) 112
MaeII ACGT 1 cut(s) 224
MalI GATC 2 cut(s) 26, 152
MboI GATC 2 cut(s) 24, 150
MboII GAAGA 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 138
MlyI GAGTC 2 cut(s) 41, 86
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 1 cut(s) 133
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 1 cut(s) 133
NdeII GATC 2 cut(s) 24, 150
NlaIII CATG 1 cut(s) 148
PkrI GCNGC 3 cut(s) 102, 172, 175
PleI GAGTC 2 cut(s) 40, 86
PpsI GAGTC 2 cut(s) 40, 86
SatI GCNGC 3 cut(s) 101, 171, 174
Sau3AI GATC 2 cut(s) 24, 150
SchI GAGTC 2 cut(s) 41, 86
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 1 cut(s) 138
SetI ASST 1 cut(s) 227
SsiI CCGC 4 cut(s) 29, 148, 171, 174
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 227
TaqI TCGA 1 cut(s) 206
TauI GCSGC 2 cut(s) 173, 176
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.