RLG00000027323
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0022395
Physical Location & Seq
Reverse (-)
10574186 .. 10575038
853 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027323

Sequence Viewer

Length: 477 bp
ATGATTCTTGCAAGGATGCAAGAGACTGCTGAGGCCTATTTCAGCTCAGCTGTTAAAAATGCAGTCGTTACTGTGCCAGTTCACTTCGATACCAAAGAAATGTTCCACTACTTGCCACAGGGGGATGGTAACTTTGAAGTGAAGGCTACTGCTGGAGACAATCACCTTGGTTGCTGCGGAAGCTGCAGATTGAGTGGGGAATGGGCCACCAGACTCTTGGATGTCACCATTCTGTCACTTGGTGTGGAGAGTGCAATGGGAGGTGCCATGATGGTTGTTATTTCAAGAAACACTCGAATTCCCATCAAGAAGAACTGTGCTTTTAGGACATCCGTCGACTACAAAACTAGTGGCAGATTCTCTGTTTATGAAGGTGATCAGAGTACGAAGGTAGTGAATAATAACTTTCTGGGTGACTATGCTCGATGGAATATATCAAGCTGCCAAGGGTGTCCCCCAGTTACAGACCTGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

159

Amino Acids

17.3

Weight (kDa)

6.81

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 71 - 146 1.9e-09 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022395)

Species Orthologous Gene IDs
malus_domestica MD08G1250700.v1.1
rosa_chinensis RchiOBHm_Chr1g0366771
rosa_laevigata RLG00000027323
rosa_samantha Rh6BG022800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AccI GTMKAC 1 cut(s) 336
AciI CCGC 1 cut(s) 177
AcsI RAATTY 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 242
AfaI GTAC 1 cut(s) 385
AgsI TTSAA 2 cut(s) 137, 285
AhlI ACTAGT 1 cut(s) 347
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AluBI AGCT 4 cut(s) 45, 50, 183, 441
AluI AGCT 4 cut(s) 45, 50, 183, 441
Alw26I GTCTC 2 cut(s) 17, 150
AoxI GGCC 2 cut(s) 33, 204
ApeKI GCWGC 3 cut(s) 174, 183, 441
ApoI RAATTY 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 204
AsuHPI GGTGA 4 cut(s) 155, 217, 386, 425
BanI GGYRCC 1 cut(s) 263
BbvCI CCTCAGC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 161, 170, 428
BccI CCATC 4 cut(s) 119, 265, 311, 420
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 2 cut(s) 17, 150
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 184
BisI GCNGC 3 cut(s) 175, 184, 442
BlpI GCTNAGC 1 cut(s) 46
BlsI GCNGC 3 cut(s) 176, 185, 443
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 1 cut(s) 265
BmrI ACTGGG 1 cut(s) 452
BmsI GCATC 1 cut(s) 6
BmuI ACTGGG 1 cut(s) 452
BoxI GACNNNNGTC 1 cut(s) 332
BpmI CTGGAG 1 cut(s) 174
Bpu10I CCTNAGC 1 cut(s) 30
Bpu1102I GCTNAGC 1 cut(s) 46
BsaJI CCNNGG 2 cut(s) 166, 445
Bse1I ACTGG 2 cut(s) 77, 458
Bse3DI GCAATG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 166, 445
BseGI GGATG 4 cut(s) 21, 130, 226, 329
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 21, 60
BseNI ACTGG 2 cut(s) 77, 458
BseXI GCAGC 3 cut(s) 161, 170, 428
BshFI GGCC 2 cut(s) 35, 206
BshNI GGYRCC 1 cut(s) 263
BslFI GGGAC 1 cut(s) 438
BsmAI GTCTC 2 cut(s) 17, 150
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 2 cut(s) 35, 206
Bsp143I GATC 1 cut(s) 376
Bsp1720I GCTNAGC 1 cut(s) 46
BspACI CCGC 1 cut(s) 177
BspANI GGCC 2 cut(s) 35, 206
BspCNI CTCAG 2 cut(s) 22, 59
BspLI GGNNCC 1 cut(s) 265
BspMAI CTGCAG 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 77, 458
BssECI CCNNGG 2 cut(s) 166, 445
BssMI GATC 1 cut(s) 376
BssT1I CCWWGG 2 cut(s) 166, 445
Bst4CI ACNGT 2 cut(s) 73, 317
BstDEI CTNAG 2 cut(s) 30, 46
BstF5I GGATG 4 cut(s) 21, 130, 226, 329
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 2 cut(s) 17, 150
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 2 cut(s) 180, 183
BstPAI GACNNNNGTC 1 cut(s) 332
BstSFI CTRYAG 1 cut(s) 184
BstV1I GCAGC 3 cut(s) 161, 170, 428
BstXI CCANNNNNNTGG 1 cut(s) 217
BsuRI GGCC 2 cut(s) 35, 206
BtsCI GGATG 4 cut(s) 21, 130, 226, 329
Cfr13I GGNCC 1 cut(s) 204
Csp6I GTAC 1 cut(s) 384
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 1 cut(s) 268
CviJI RGCY 7 cut(s) 35, 45, 50, 146, 183, 206, 441
CviKI_1 RGCY 7 cut(s) 35, 45, 50, 146, 183, 206, 441
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 2 cut(s) 30, 46
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
DraIII CACNNNGTG 1 cut(s) 242
Eco130I CCWWGG 2 cut(s) 166, 445
Eco147I AGGCCT 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 297
EcoT14I CCWWGG 2 cut(s) 166, 445
ErhI CCWWGG 2 cut(s) 166, 445
FaeI CATG 1 cut(s) 271
FaiI YATR 4 cut(s) 269, 369, 420, 434
FaqI GGGAC 1 cut(s) 438
FatI CATG 1 cut(s) 267
FbaI TGATCA 1 cut(s) 376
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 3 cut(s) 175, 184, 442
FokI GGATG 4 cut(s) 28, 137, 233, 316
Fsp4HI GCNGC 3 cut(s) 175, 184, 442
FspBI CTAG 1 cut(s) 348
GluI GCNGC 3 cut(s) 175, 184, 442
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 2 cut(s) 35, 206
Hin1II CATG 1 cut(s) 271
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 3 cut(s) 4, 213, 357
HphI GGTGA 4 cut(s) 155, 217, 386, 425
Hpy166II GTNNAC 2 cut(s) 82, 337
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 2 cut(s) 285, 307
Hpy8I GTNNAC 2 cut(s) 82, 337
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 3 cut(s) 136, 365, 382
HpyCH4III ACNGT 2 cut(s) 73, 317
HpyCH4V TGCA 5 cut(s) 11, 19, 62, 186, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 183
HpyF3I CTNAG 2 cut(s) 30, 46
Hsp92II CATG 1 cut(s) 271
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 1 cut(s) 376
LpnPI CCDG 6 cut(s) 90, 104, 138, 223, 395, 471
Lsp1109I GCAGC 3 cut(s) 161, 170, 428
LweI GCATC 1 cut(s) 6
MaeI CTAG 1 cut(s) 348
MaeIII GTNAC 6 cut(s) 67, 128, 223, 234, 413, 460
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 1 cut(s) 322
MluCI AATT 1 cut(s) 297
MlyI GAGTC 1 cut(s) 207
MnlI CCTC 2 cut(s) 25, 254
MseI TTAA 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 180, 183
NdeII GATC 1 cut(s) 376
NlaIII CATG 1 cut(s) 271
NlaIV GGNNCC 1 cut(s) 265
NmuCI GTSAC 3 cut(s) 223, 234, 413
PceI AGGCCT 1 cut(s) 35
PfeI GAWTC 2 cut(s) 4, 357
PkrI GCNGC 3 cut(s) 176, 185, 443
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
PshAI GACNNNNGTC 1 cut(s) 332
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 204
PstI CTGCAG 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SalI GTCGAC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 3 cut(s) 175, 184, 442
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 1 cut(s) 207
SetI ASST 9 cut(s) 47, 52, 168, 185, 265, 376, 393, 443, 471
SfaNI GCATC 1 cut(s) 6
SfcI CTRYAG 1 cut(s) 184
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 1 cut(s) 297
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 1 cut(s) 177
SspMI CTAG 1 cut(s) 348
StuI AGGCCT 1 cut(s) 35
StyI CCWWGG 2 cut(s) 166, 445
TaaI ACNGT 2 cut(s) 73, 317
TaqI TCGA 4 cut(s) 87, 295, 336, 424
TasI AATT 1 cut(s) 297
TfiI GAWTC 2 cut(s) 4, 357
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TseFI GTSAC 3 cut(s) 223, 234, 413
TseI GCWGC 3 cut(s) 174, 183, 441
Tsp45I GTSAC 3 cut(s) 223, 234, 413
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 322
XapI RAATTY 1 cut(s) 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.