MD09G1041400.v1.1

Paired amphipathic helix protein Sin3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0019127
Physical Location & Seq
Reverse (-)
2670021 .. 2670697
677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1041400.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGTTTTGAAAGGTGAATTTCAGAGCTTTATGAATTTTGTCATGTATATCTGTCGTTCTTCCAACTTTTTTTTTGGATTATATAAATTTTTTATCTTAGATAATTATATTATCAAATTTACGAATTTCTTTTGTTCCTTTCTGGATTTGGAGCACAGCAATGGGCGATCCCGATTACCCAGCGGTGGAGGAGGCGGTGATTCTCAGAAATTGACAACCGCCGATGCTCTTTTGTTTCTCAAACAAGTGAAGGATGCGTTTTGTGACAAAGAAGAAAATTATGAGAAATTTCTCGAGATCATGAAAGATTTCAAGGCTAAAAGGGCTGTCACAGAAGATGTGGTTGCACGAGTGAAGGAATTATTCAAAGGGCATAAGGATTTAATTTTGGGATTTAATCCCTTCTTACCGGAGGGTTATCAGATAACCCCGGATGACGACGAAGATGCAACTTCGCTGAAACAGACAGTTGATGAAGATGAAGAAGCTGCTGGCTCTATAAACTTGACAAACGTGAGTGAGTACAAATATATTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202
Pfam Domains
Protein Families
PAH Sin3_corepress Sin3a_C

Protein Analysis

180

Amino Acids

20.56

Weight (kDa)

4.84

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAH PF02671 92 - 136 4.9e-17 Paired amphipathic helix repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15025
malus_domestica MD09G1041400.v1.1 MD15G1355900.v1.1
prunus_persica Prupe.3G276200_v2.0.a1 Prupe.3G276200_v2.0.a1
pyrus_communis pycom111g03240
rosa_laevigata RLG00000021909
rosa_samantha Rh2AG616300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 183, 195, 219
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 6 cut(s) 17, 34, 86, 116, 124, 287
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 3 cut(s) 10, 313, 367
AluBI AGCT 2 cut(s) 27, 488
AluI AGCT 2 cut(s) 27, 488
Alw21I GWGCWC 1 cut(s) 156
AlwI GGATC 1 cut(s) 162
Ama87I CYCGRG 1 cut(s) 293
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 6 cut(s) 17, 34, 86, 116, 124, 287
Asp700I GAANNNNTTC 1 cut(s) 308
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 2 cut(s) 26, 209
AvaI CYCGRG 1 cut(s) 293
BauI CACGAG 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 475
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BcnI CCSGG 1 cut(s) 431
BisI GCNGC 1 cut(s) 489
BlsI GCNGC 1 cut(s) 490
Bme1390I CCNGG 1 cut(s) 431
BmeT110I CYCGRG 1 cut(s) 293
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 3 cut(s) 214, 244, 436
BpuMI CCSGG 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 429
BsaWI WCCGGW 1 cut(s) 409
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse3DI GCAATG 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 2 cut(s) 259, 439
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 218
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 475
BseYI CCCAGC 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 2 cut(s) 410, 431
BslI CCNNNNNNNGG 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 2 cut(s) 167, 297
BspACI CCGC 3 cut(s) 183, 195, 219
BspCNI CTCAG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 300
BspPI GGATC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 2 cut(s) 167, 297
BssSI CACGAG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 469
BstC8I GCNNGC 1 cut(s) 493
BstDEI CTNAG 2 cut(s) 97, 204
BstF5I GGATG 2 cut(s) 259, 439
BstKTI GATC 2 cut(s) 170, 300
BstMBI GATC 2 cut(s) 167, 297
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 475
BtsCI GGATG 2 cut(s) 259, 439
Cac8I GCNNGC 1 cut(s) 493
CciI TCATGA 1 cut(s) 300
Csp6I GTAC 1 cut(s) 523
CviAII CATG 2 cut(s) 43, 301
CviJI RGCY 5 cut(s) 27, 317, 326, 488, 495
CviKI_1 RGCY 5 cut(s) 27, 317, 326, 488, 495
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 2 cut(s) 97, 204
DpnI GATC 2 cut(s) 169, 299
DpnII GATC 2 cut(s) 167, 297
Eco88I CYCGRG 1 cut(s) 293
FaeI CATG 2 cut(s) 46, 304
FatI CATG 2 cut(s) 42, 300
Fnu4HI GCNGC 1 cut(s) 489
FokI GGATG 2 cut(s) 266, 446
Fsp4HI GCNGC 1 cut(s) 489
GluI GCNGC 1 cut(s) 489
GsaI CCCAGC 1 cut(s) 183
HapII CCGG 2 cut(s) 410, 431
Hin1II CATG 2 cut(s) 46, 304
HinfI GANTC 1 cut(s) 200
HpaII CCGG 2 cut(s) 410, 431
HphI GGTGA 2 cut(s) 26, 209
Hpy188I TCNGA 3 cut(s) 24, 207, 423
Hpy188III TCNNGA 5 cut(s) 143, 171, 293, 295, 301
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 3 cut(s) 244, 349, 412
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 1 cut(s) 513
HpyCH4V TGCA 2 cut(s) 347, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 2 cut(s) 97, 204
HpySE526I ACGT 1 cut(s) 513
Hsp92II CATG 2 cut(s) 46, 304
Kzo9I GATC 2 cut(s) 167, 297
LmnI GCTCC 1 cut(s) 151
LpnPI CCDG 5 cut(s) 128, 193, 423, 444, 477
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 3 cut(s) 214, 244, 436
MaeII ACGT 1 cut(s) 513
MaeIII GTNAC 2 cut(s) 263, 328
MalI GATC 2 cut(s) 169, 299
MboI GATC 2 cut(s) 167, 297
MboII GAAGA 6 cut(s) 51, 284, 347, 455, 488, 494
MhlI GDGCHC 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 3 cut(s) 182, 185, 406
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 3 cut(s) 383, 396, 538
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 2 cut(s) 410, 431
MspR9I CCNGG 1 cut(s) 431
MwoI GCNNNNNNNGC 1 cut(s) 323
NciI CCSGG 1 cut(s) 431
NdeII GATC 2 cut(s) 167, 297
NlaIII CATG 2 cut(s) 46, 304
NmuCI GTSAC 2 cut(s) 263, 328
PaeR7I CTCGAG 1 cut(s) 293
PagI TCATGA 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 179
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 3 cut(s) 383, 396, 538
SatI GCNGC 1 cut(s) 489
Sau3AI GATC 2 cut(s) 167, 297
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 1 cut(s) 156
SetI ASST 4 cut(s) 16, 29, 490, 516
SfaNI GCATC 3 cut(s) 214, 244, 436
Sfr274I CTCGAG 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SsiI CCGC 3 cut(s) 183, 195, 219
StyD4I CCNGG 1 cut(s) 429
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 1 cut(s) 516
TaqI TCGA 1 cut(s) 294
TatI WGTACW 1 cut(s) 522
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 3 cut(s) 383, 396, 538
Tru9I TTAA 3 cut(s) 383, 396, 538
TseFI GTSAC 2 cut(s) 263, 328
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 2 cut(s) 263, 328
TspDTI ATGAA 4 cut(s) 47, 317, 489, 495
XapI RAATTY 6 cut(s) 17, 34, 86, 116, 124, 287
XhoI CTCGAG 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.