MD09G1058400.v1.1

potassium channel activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0021156
Physical Location & Seq
Forward (+)
3879898 .. 3880758
861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1058400.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGGGGTAAGGCTAGCTGACATTTACCTCTCCCAGACCCTGCGTAAAGCGGAAGCCTTGTGCACTGGGTACGACCTTTTTATTGTTTTTAGTGCTGGATTTGAAGCAATGTCAGTTTTTCAGTGCATAATCAAGTTTCAGCCTGTAAATGTTATAGGTGGAGGAGCTATGCATCGCCAAAGTCGAGCCATATTTGGAGGTGGTGGTGAAGGGATAGGGGGAAGAGAGGGGAGAGAGGTAGGTGCAGTAGGTGGCAGAAGTTGGGCGTGGAAGTTGTTTAGTTGGCCAGTCTTGTCCATCGCAATATACATATTTTTCTTTTTCTTGAGTGTAGACTTTGTTGATTTGAATGAACGCCTTCCGAGTGAACACCCTACTAAGAAATCAGTTAAGGCACTATTCCTCATGTCCGAAACATTGACCGGTGTGGGGTACGGTGACATTGTCCCCAACACTGAGCGGGCCAAGTTGTTAATATCCTTTTTTATTTTCTTCGTCCGTTGGATTTGGTGCAAATCAGGAGGAGTTTTTTTGGTGTCTATTTGTGAAGGTTTTCTCAAACGATTTGGGTTTTGTGGTGTGAGTGAGGACATTTTCAGGGTAGTGATGGCAATAGCGGCACCTGCTGTGTGTGTGGGAGTAGGCTGTGTTGGGTTTCACTTGTTGGAGAAGCTGAGTTGGGAGGATGCTTTGTATTTGTCAGTTGTTTCTGCGACCACTGTTGGCTACGGTGAAATTCCGGTAAAGACAGCAAGCACTAAGGTTTTTGCAAGCATTTGGATGTGTTTTTCCACAATTATCTTTATCAAATGTCAGACATACCTAGTTGTTAGAATTATGAGAGCGTTTGGAAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

287

Amino Acids

31.58

Weight (kDa)

8.96

Isoelectric Point (pI)

29.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 128 - 165 2.2e-06 Ion channel
Ion_trans_2 PF07885 215 - 277 1.8e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021156)

Species Orthologous Gene IDs
malus_domestica MD09G1058100.v1.1 MD09G1058400.v1.1 MD09G1058700.v1.1
prunus_persica Prupe.1G192600_v2.0.a1
pyrus_communis pycom111g04840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 631
Acc36I ACCTGC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 619
AccBSI CCGCTC 1 cut(s) 460
AccI GTMKAC 1 cut(s) 333
AciI CCGC 3 cut(s) 50, 460, 617
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 1 cut(s) 735
AfaI GTAC 2 cut(s) 71, 434
AfiI CCNNNNNNNGG 1 cut(s) 429
AgeI ACCGGT 1 cut(s) 422
AgsI TTSAA 2 cut(s) 104, 349
AluBI AGCT 3 cut(s) 17, 167, 673
AluI AGCT 3 cut(s) 17, 167, 673
Alw21I GWGCWC 1 cut(s) 65
Alw44I GTGCAC 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 40
AoxI GGCC 2 cut(s) 284, 462
ApaLI GTGCAC 1 cut(s) 61
ApoI RAATTY 1 cut(s) 735
AsiGI ACCGGT 1 cut(s) 422
Asp700I GAANNNNTTC 2 cut(s) 357, 552
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 3 cut(s) 218, 449, 743
AsuNHI GCTAGC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 65
BalI TGGCCA 1 cut(s) 286
BanI GGYRCC 1 cut(s) 619
Bbv12I GWGCWC 1 cut(s) 65
BccI CCATC 2 cut(s) 305, 601
BfaI CTAG 2 cut(s) 14, 824
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 1 cut(s) 618
BlsI GCNGC 1 cut(s) 619
BmgT120I GGNCC 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 621
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 181, 676
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 346
BsaWI WCCGGW 2 cut(s) 422, 739
Bsc4I CCNNNNNNNGG 1 cut(s) 429
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 70, 287
Bse3DI GCAATG 1 cut(s) 114
BseGI GGATG 2 cut(s) 691, 786
BseLI CCNNNNNNNGG 1 cut(s) 429
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 2 cut(s) 447, 665
BseNI ACTGG 2 cut(s) 70, 287
BseRI GAGGAG 2 cut(s) 177, 537
BseSI GKGCMC 1 cut(s) 65
BsgI GTGCAG 1 cut(s) 264
BshFI GGCC 2 cut(s) 286, 464
BshNI GGYRCC 1 cut(s) 619
BshTI ACCGGT 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 423, 740
BslFI GGGAC 1 cut(s) 431
BslI CCNNNNNNNGG 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 431
BsnI GGCC 2 cut(s) 286, 464
Bsp1286I GDGCHC 1 cut(s) 65
BspACI CCGC 3 cut(s) 50, 460, 617
BspANI GGCC 2 cut(s) 286, 464
BspCNI CTCAG 2 cut(s) 448, 666
BspLI GGNNCC 1 cut(s) 621
BspMI ACCTGC 1 cut(s) 631
BspOI GCTAGC 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 619
BsrBI CCGCTC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 2 cut(s) 70, 287
BssAI RCCGGY 1 cut(s) 422
Bst4CI ACNGT 3 cut(s) 437, 721, 731
Bst6I CTCTTC 1 cut(s) 217
BstC8I GCNNGC 4 cut(s) 15, 462, 754, 772
BstDEI CTNAG 4 cut(s) 378, 456, 674, 759
BstF5I GGATG 2 cut(s) 691, 786
BstMWI GCNNNNNNNGC 2 cut(s) 617, 623
BstSLI GKGCMC 1 cut(s) 65
BsuRI GGCC 2 cut(s) 286, 464
BtgZI GCGATG 2 cut(s) 158, 283
BtsCI GGATG 2 cut(s) 691, 786
BtsIMutI CAGTG 4 cut(s) 63, 128, 453, 717
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 4 cut(s) 15, 462, 754, 772
CaiI CAGNNNCTG 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 462
Csp6I GTAC 2 cut(s) 70, 433
CspAI ACCGGT 1 cut(s) 422
CviAII CATG 1 cut(s) 406
CviQI GTAC 2 cut(s) 70, 433
DdeI CTNAG 4 cut(s) 378, 456, 674, 759
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
EcoT22I ATGCAT 1 cut(s) 174
FaeI CATG 1 cut(s) 409
FaiI YATR 9 cut(s) 128, 155, 170, 191, 307, 311, 407, 820, 839
FaqI GGGAC 1 cut(s) 431
FatI CATG 1 cut(s) 405
FauI CCCGC 1 cut(s) 453
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 1 cut(s) 618
FokI GGATG 2 cut(s) 698, 793
Fsp4HI GCNGC 1 cut(s) 618
FspBI CTAG 2 cut(s) 14, 824
GluI GCNGC 1 cut(s) 618
HaeIII GGCC 2 cut(s) 286, 464
HapII CCGG 2 cut(s) 423, 740
Hin1II CATG 1 cut(s) 409
HpaII CCGG 2 cut(s) 423, 740
HphI GGTGA 3 cut(s) 218, 449, 743
Hpy166II GTNNAC 3 cut(s) 63, 334, 368
Hpy188I TCNGA 3 cut(s) 363, 412, 816
Hpy188III TCNNGA 2 cut(s) 325, 519
Hpy8I GTNNAC 3 cut(s) 63, 334, 368
HpyAV CCTTC 3 cut(s) 203, 368, 542
HpyCH4III ACNGT 3 cut(s) 437, 721, 731
HpyCH4V TGCA 6 cut(s) 63, 126, 172, 245, 513, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 623
HpyF3I CTNAG 4 cut(s) 378, 456, 674, 759
Hsp92II CATG 1 cut(s) 409
LmnI GCTCC 1 cut(s) 164
LweI GCATC 2 cut(s) 181, 676
MaeI CTAG 2 cut(s) 14, 824
MaeIII GTNAC 1 cut(s) 437
MbiI CCGCTC 1 cut(s) 460
MboII GAAGA 2 cut(s) 234, 484
MhlI GDGCHC 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 4 cut(s) 735, 795, 834, 856
MluNI TGGCCA 1 cut(s) 286
MmeI TCCRAC 2 cut(s) 482, 645
MnlI CCTC 9 cut(s) 38, 155, 191, 220, 229, 413, 515, 580, 676
Mox20I TGGCCA 1 cut(s) 286
Mph1103I ATGCAT 1 cut(s) 174
MroXI GAANNNNTTC 2 cut(s) 357, 552
MscI TGGCCA 1 cut(s) 286
MseI TTAA 3 cut(s) 390, 473, 859
MslI CAYNNNNRTG 1 cut(s) 779
Msp20I TGGCCA 1 cut(s) 286
MspI CCGG 2 cut(s) 423, 740
MwoI GCNNNNNNNGC 2 cut(s) 617, 623
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 409
NlaIV GGNNCC 1 cut(s) 621
NmuCI GTSAC 1 cut(s) 437
NsiI ATGCAT 1 cut(s) 174
PaqCI CACCTGC 1 cut(s) 631
PcsI WCGNNNNNNNCGW 1 cut(s) 181
PdmI GAANNNNTTC 2 cut(s) 357, 552
PflFI GACNNNGTC 1 cut(s) 443
PinAI ACCGGT 1 cut(s) 422
PkrI GCNGC 1 cut(s) 619
PspN4I GGNNCC 1 cut(s) 621
PspPI GGNCC 1 cut(s) 462
PstNI CAGNNNCTG 1 cut(s) 40
PsyI GACNNNGTC 1 cut(s) 443
RsaI GTAC 2 cut(s) 71, 434
RsaNI GTAC 2 cut(s) 70, 433
RseI CAYNNNNRTG 1 cut(s) 779
SaqAI TTAA 3 cut(s) 390, 473, 859
SatI GCNGC 1 cut(s) 618
Sau96I GGNCC 1 cut(s) 462
SduI GDGCHC 1 cut(s) 65
SfaNI GCATC 2 cut(s) 181, 676
SmiMI CAYNNNNRTG 1 cut(s) 779
SmlI CTYRAG 1 cut(s) 325
SmoI CTYRAG 1 cut(s) 325
Sse9I AATT 4 cut(s) 735, 795, 834, 856
SsiI CCGC 3 cut(s) 50, 460, 617
SspMI CTAG 2 cut(s) 14, 824
TaaI ACNGT 3 cut(s) 437, 721, 731
TaqI TCGA 1 cut(s) 184
TasI AATT 4 cut(s) 735, 795, 834, 856
TauI GCSGC 1 cut(s) 620
Tru1I TTAA 3 cut(s) 390, 473, 859
Tru9I TTAA 3 cut(s) 390, 473, 859
TscAI CASTG 4 cut(s) 70, 128, 460, 724
TseFI GTSAC 1 cut(s) 437
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 1 cut(s) 366
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 4 cut(s) 70, 128, 460, 724
Tth111I GACNNNGTC 1 cut(s) 443
VneI GTGCAC 1 cut(s) 61
XapI RAATTY 1 cut(s) 735
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 357, 552
XspI CTAG 2 cut(s) 14, 824
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.