MD09G1058700.v1.1

potassium channel activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0021156
Physical Location & Seq
Reverse (-)
3894527 .. 3895828
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1058700.v1.1.491

Sequence Viewer

Length: 702 bp
ATGAATCAATTCCCAGGTGAGGGAGCTATGCATCGCCAAAATCGAGCCATTGGAGGTGCTGGAGTAGGGATAGGGGAGAGAGAGGGAAGTGCAGGTGGAAAGAGTTGGGCTTGGAAGATGTTTAGTTGGTTAGTCTTATCTATCTTCGTTTATATATTTTTTTTGTTGTTGAGTGGCGACTTTGCTGATCATAGCAAATTCCATACTAGAAAGTCTGTTAGGGCACTATACCTAATGTCTCAAACAATGACCGGTGTGGGGTACGGTGACATCGTCCCCAAAACTGAGCGGTCAAAGGTGTTAGTATCCTTTCTGATTTTCTTCGTCCGTTGGATTTGGTGCTACTCAGGAGGTGTTTTTTTTGCTTTTATCGGTGAGGGTTTCCTCAGACGATATAGGAAGGTAGTAAACTTTTTTAGTGTGAGTGAGGACCGTTTTAGGGTAGTGATGGCAGTAGCGGCACCTGCTCTGTGTGTGGGAGTAGGCTGTATTGGGTTTCACTTCTTGGGGAAGAAGAGCTGGGAGAATGCTATGTATTTGTCGGTTGTTTCTGTAACCACTGTAGGCTATGGTGAAGTTACGGTGGAGACGGCAGGCGCTAGGGTTTTTGCAAGCATTTGGATGTGCCTTTCCACAGTTATTTTTCTTAAATGTCAGACGTACCTAGTTGTTAGAATTATGAGCGGGTTTAGAAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

234

Amino Acids

26.12

Weight (kDa)

9.85

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 44 - 109 8.4e-08 Ion transport protein
Ion_trans_2 PF07885 47 - 116 2.7e-07 Ion channel
Ion_trans_2 PF07885 162 - 223 7.4e-12 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021156)

Species Orthologous Gene IDs
malus_domestica MD09G1058100.v1.1 MD09G1058400.v1.1 MD09G1058700.v1.1
prunus_persica Prupe.1G192600_v2.0.a1
pyrus_communis pycom111g04840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 83, 472
Acc36I ACCTGC 2 cut(s) 83, 472
AccB1I GGYRCC 1 cut(s) 460
AccBSI CCGCTC 2 cut(s) 289, 684
AciI CCGC 3 cut(s) 289, 458, 684
AcsI RAATTY 1 cut(s) 197
AfaI GTAC 2 cut(s) 263, 662
AfiI CCNNNNNNNGG 4 cut(s) 19, 20, 258, 439
AgeI ACCGGT 1 cut(s) 251
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 2 cut(s) 26, 519
AluI AGCT 2 cut(s) 26, 519
Alw26I GTCTC 2 cut(s) 243, 581
ApoI RAATTY 1 cut(s) 197
AsiGI ACCGGT 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 599
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 4 cut(s) 29, 278, 386, 584
AvaII GGWCC 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 460
BccI CCATC 1 cut(s) 442
BceAI ACGGC 1 cut(s) 606
BciT130I CCWGG 1 cut(s) 15
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 187
BcoDI GTCTC 2 cut(s) 243, 581
BfaI CTAG 3 cut(s) 207, 600, 665
BfmI CTRYAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 600
BfuAI ACCTGC 2 cut(s) 83, 472
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 13
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 20, 258, 439
Bse118I RCCGGY 1 cut(s) 251
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 13
BseGI GGATG 1 cut(s) 627
BseLI CCNNNNNNNGG 4 cut(s) 19, 20, 258, 439
BseMII CTCAG 3 cut(s) 276, 360, 400
BseSI GKGCMC 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 519
BsgI GTGCAG 1 cut(s) 111
BshNI GGYRCC 1 cut(s) 460
BshTI ACCGGT 1 cut(s) 251
BsiSI CCGG 1 cut(s) 252
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 4 cut(s) 19, 20, 258, 439
BsmAI GTCTC 2 cut(s) 243, 581
BsmBI CGTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 532
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 1 cut(s) 187
BspACI CCGC 3 cut(s) 289, 458, 684
BspCNI CTCAG 3 cut(s) 277, 359, 399
BspLI GGNNCC 1 cut(s) 462
BspMI ACCTGC 2 cut(s) 83, 472
BspQI GCTCTTC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 460
BsrBI CCGCTC 2 cut(s) 289, 684
BsrFI RCCGGY 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 13
BssMI GATC 1 cut(s) 187
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 5 cut(s) 266, 434, 562, 583, 637
Bst6I CTCTTC 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 595, 613
BstDEI CTNAG 3 cut(s) 285, 346, 386
BstF5I GGATG 1 cut(s) 627
BstH2I RGCGCY 1 cut(s) 600
BstHHI GCGC 1 cut(s) 599
BstKTI GATC 1 cut(s) 190
BstMAI GTCTC 2 cut(s) 243, 581
BstMBI GATC 1 cut(s) 187
BstMWI GCNNNNNNNGC 2 cut(s) 458, 464
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 226
BsuI GTATCC 1 cut(s) 316
BtgZI GCGATG 1 cut(s) 17
BtsCI GGATG 1 cut(s) 627
BtsIMutI CAGTG 1 cut(s) 558
BveI ACCTGC 2 cut(s) 83, 472
Cac8I GCNNGC 2 cut(s) 595, 613
CfoI GCGC 1 cut(s) 599
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 262, 661
CspAI ACCGGT 1 cut(s) 251
CviJI RGCY 6 cut(s) 26, 47, 110, 486, 519, 567
CviKI_1 RGCY 6 cut(s) 26, 47, 110, 486, 519, 567
CviQI GTAC 2 cut(s) 262, 661
DdeI CTNAG 3 cut(s) 285, 346, 386
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 13
EcoT22I ATGCAT 1 cut(s) 33
Esp3I CGTCTC 1 cut(s) 581
FaqI GGGAC 1 cut(s) 260
FauI CCCGC 1 cut(s) 677
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 1 cut(s) 634
Fsp4HI GCNGC 1 cut(s) 459
FspBI CTAG 3 cut(s) 207, 600, 665
GlaI GCGC 1 cut(s) 598
GluI GCNGC 1 cut(s) 459
GsaI CCCAGC 1 cut(s) 523
GsuI CTGGAG 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 600
HapII CCGG 1 cut(s) 252
HhaI GCGC 1 cut(s) 599
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 252
HphI GGTGA 4 cut(s) 29, 278, 386, 584
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 3 cut(s) 315, 389, 657
Hpy188III TCNNGA 1 cut(s) 348
Hpy8I GTNNAC 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 394
HpyCH4III ACNGT 5 cut(s) 266, 434, 562, 583, 637
HpyCH4IV ACGT 1 cut(s) 659
HpyCH4V TGCA 3 cut(s) 31, 92, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 464
HpyF3I CTNAG 3 cut(s) 285, 346, 386
HpySE526I ACGT 1 cut(s) 659
HspAI GCGC 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 1 cut(s) 187
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 8 cut(s) 27, 45, 78, 265, 333, 477, 505, 579
LweI GCATC 1 cut(s) 40
MaeI CTAG 3 cut(s) 207, 600, 665
MaeII ACGT 1 cut(s) 659
MaeIII GTNAC 3 cut(s) 266, 553, 577
MalI GATC 1 cut(s) 189
MbiI CCGCTC 2 cut(s) 289, 684
MboI GATC 1 cut(s) 187
MboII GAAGA 5 cut(s) 127, 136, 313, 523, 526
MhlI GDGCHC 1 cut(s) 226
MluCI AATT 4 cut(s) 8, 197, 675, 697
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 7 cut(s) 13, 47, 76, 344, 370, 395, 421
Mph1103I ATGCAT 1 cut(s) 33
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 1 cut(s) 620
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 458, 464
NdeII GATC 1 cut(s) 187
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 1 cut(s) 266
NsiI ATGCAT 1 cut(s) 33
PaqCI CACCTGC 2 cut(s) 83, 472
PciSI GCTCTTC 1 cut(s) 509
PcsI WCGNNNNNNNCGW 2 cut(s) 40, 270
PctI GAATGC 1 cut(s) 532
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 4
PflFI GACNNNGTC 1 cut(s) 272
PinAI ACCGGT 1 cut(s) 251
PkrI GCNGC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 519
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 1 cut(s) 430
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 2 cut(s) 263, 662
RsaNI GTAC 2 cut(s) 262, 661
RseI CAYNNNNRTG 1 cut(s) 620
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 1 cut(s) 459
Sau3AI GATC 1 cut(s) 187
Sau96I GGNCC 1 cut(s) 430
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 226
SfaNI GCATC 1 cut(s) 40
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 620
Sse9I AATT 4 cut(s) 8, 197, 675, 697
SsiI CCGC 3 cut(s) 289, 458, 684
SspMI CTAG 3 cut(s) 207, 600, 665
StyD4I CCNGG 1 cut(s) 13
TaaI ACNGT 5 cut(s) 266, 434, 562, 583, 637
TaiI ACGT 1 cut(s) 662
TaqI TCGA 1 cut(s) 43
TasI AATT 4 cut(s) 8, 197, 675, 697
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 1 cut(s) 565
TseFI GTSAC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 565
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 3 cut(s) 207, 600, 665
Zsp2I ATGCAT 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.