Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004932

Physical Location & Seq

Genomic Coordinates Forward (+)

5777674 .. 5782245

Gene Structure 4572 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1082300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGGCGTCGGAAACCGATCGTCTTCTCCGGCAATCTTCCTTTTCCGCGGCGGAAGACATGGCGGAAGATGCCGACGCATCGAAGCCCTTCCTTTGTAGGTTTTTGAGCTATCCTGTTGCTAATACCAATACATCGAGCCACAAGAGGCGTCGCAGGCTCGCCGAGAAATTCGCCTCCACCGCCCCCCGGCGGCTGACTCGTCGGCAGCAGTCTTTCAGCCGCGACATCGGCCACGCTGCCAAGGAGACTTACTTGATTACTAGCCTCAGCTTCACGCTCCTTCGATACCTCGGGGTAGGCTACCGGTGGATGACGAGATTGCTTGCTCTTGGTTGCTATGCTATGTTACTTATGCCAGGATTTCTTCAAGTTGCAATATGTTATTTCTTTTCAAGCCAGGTCTGGCGTAGTATTGTGTATGGAGATCAACCAAGAAATAGGTTGGATCTATATTTACCTGCAAATAGTGATGGAAAAAAGCCGGTAGTGGTATTTGTAACTGGTGGAGCCTGGATTATTGGGTATAAAGCTTGGGGTTCCCTTTTAGGTCTGCAGTTGGCTGAAAGAGACATCATTGTGGCATGCGTTGATTACAGAAACTTTCCGCAAGGTACCATCAGTGATATGGTGAAAGATGCTTCTCAGGGGATCTCATATGTCTGCAACAATATAGCAGATTATGGAGGTGACCCTAATAGGATCTATCTAATGGGACAATCTGCTGGTGCGCATATTTCTGCTTGTGCTCTCTTGGATCAAGCAATCAAAGAAGCTAAGAAGGAAGAAAGCATTTCTTGGAGCGTCTCCCAGATAAAGGCTTATTTTGGTTTATCTGGCGGGTACAATTTGTTTAATTTAGTTGATCATTTCCATAATCGTGGACTATATCGTTCCATCTTTTTGAGCATTATGGAAGGTGAAGAATCCTTACATCAGTTTTCTCCTGAAGTTAAAGTAAACGACCCAAGCAATAGGAATGCCGTTTCTCTATTGCCTCCTATCACTCTTTTCCATGGAACTGCAGATTATTCGATACCATCTGACGCCAGTAAGGTTTTTGTGGACGTGCTTCAGAAGGCAGGAGCTAAAGCTGACTTAATACTATATGATGGAAAGACTCATACAGATTTGTTTCTTCAAGATCCTCTAAGAGGCGGCAAAGATGAATTGTTTGACCAACTAGTTTCCGTGATACATGCTGGTGATACCGAAGCTCTTGAGCAAGATGCTATTGCACCTCCGAGAAGGCGCCTTGTTCCAGAGCTCTTGTTGAAGTTGGCACGCTGCATCAGCCCCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0008150 GO:0008152 GO:0009056 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010296 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0031090 GO:0031984 GO:0033993 GO:0042175 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051723 GO:0052689 GO:0097305 GO:0098588 GO:0098791 GO:0098827 GO:0140096 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00900 ko01120 ko01130 map00900 map01120 map01130

Pfam Domains

Protein Families

Abhydrolase_3 COesterase

Protein Analysis

434

Amino Acids

48.25

Weight (kDa)

8.35

Isoelectric Point (pI)

49.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BD-FAE	PF20434	148 - 355	4.4e-28	BD-FAE
COesterase	PF00135	148 - 254	1.7e-14	Carboxylesterase family
Abhydrolase_3	PF07859	163 - 374	3.3e-17	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02410 AT3G02410 AT3G02410 AT5G15860
fragaria_vesca	FvH4_6g45510 FvH4_6g45510 FvH4_6g45510 FvH4_6g45510 FvH4_6g45510 FvH4_6g45510 FvH4_6g45510
malus_domestica	MD09G1082300.v1.1 MD17G1073000.v1.1
prunus_persica	Prupe.3G241400_v2.0.a1 Prupe.3G241400_v2.0.a1 Prupe.3G241400_v2.0.a1 Prupe.3G241400_v2.0.a1
pyrus_communis	pycom09g00880 pycom17g07390
rosa_chinensis	RchiOBHm_Chr2g0163451
rosa_laevigata	RLG00000021402
rosa_multiflora	Rmu_sc0028601.1_g000003
rosa_roxburghii	Rroxscaffold_2G00087310
rosa_rugosa	Rorug02G0502800 Rorug02G0502900
rosa_samantha	Rh2AG569800 Rh2BG582000 Rh2CG551800 Rh2DG591700
rosa_wichuraiana	Rw2G047170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	729
Acc36I	ACCTGC	1 cut(s)	466
Acc65I	GGTACC	1 cut(s)	611
AccB1I	GGYRCC	2 cut(s)	611, 1248
AccII	CGCG	2 cut(s)	47, 222
AclWI	GGATC	5 cut(s)	453, 656, 707, 762, 1136
AcoI	YGGCCR	1 cut(s)	229
AcsI	RAATTY	1 cut(s)	167
AcuI	CTGAAG	2 cut(s)	966, 1055
AcyI	GRCGYC	4 cut(s)	5, 148, 1044, 1249
AfaI	GTAC	2 cut(s)	613, 842
AfiI	CCNNNNNNNGG	4 cut(s)	186, 189, 814, 1151
AgeI	ACCGGT	1 cut(s)	303
AgsI	TTSAA	4 cut(s)	368, 393, 1139, 1273
AhlI	ACTAGT	1 cut(s)	1180
AjiI	CACGTC	1 cut(s)	1066
AjnI	CCWGG	3 cut(s)	355, 396, 509
AluBI	AGCT	8 cut(s)	108, 270, 530, 773, 1085, 1091, 1214, 1264
AluI	AGCT	8 cut(s)	108, 270, 530, 773, 1085, 1091, 1214, 1264
Alw21I	GWGCWC	2 cut(s)	748, 1266
Alw26I	GTCTC	3 cut(s)	239, 561, 808
AlwI	GGATC	5 cut(s)	453, 656, 707, 762, 1136
Ama87I	CYCGRG	1 cut(s)	290
AoxI	GGCC	1 cut(s)	229
ApeKI	GCWGC	3 cut(s)	205, 236, 1284
ApoI	RAATTY	1 cut(s)	167
AsiGI	ACCGGT	1 cut(s)	303
Asp700I	GAANNNNTTC	1 cut(s)	86
Asp718I	GGTACC	1 cut(s)	611
AspLEI	GCGC	2 cut(s)	730, 1251
AsuC2I	CCSGG	1 cut(s)	187
AsuHPI	GGTGA	4 cut(s)	640, 698, 929, 1214
AvaI	CYCGRG	1 cut(s)	290
BanI	GGYRCC	2 cut(s)	611, 1248
BanII	GRGCYC	1 cut(s)	1266
BarI	GAAGNNNNNNTAC	2 cut(s)	912, 944
BbsI	GAAGAC	2 cut(s)	14, 60
Bbv12I	GWGCWC	2 cut(s)	748, 1266
BbvCI	CCTCAGC	1 cut(s)	266
BbvI	GCAGC	3 cut(s)	217, 223, 1271
BccI	CCATC	5 cut(s)	464, 623, 902, 1045, 1103
BceAI	ACGGC	1 cut(s)	965
BcgI	CGANNNNNNTGC	2 cut(s)	1011, 1045
BciT130I	CCWGG	3 cut(s)	357, 398, 511
BclI	TGATCA	1 cut(s)	862
BcnI	CCSGG	1 cut(s)	187
BcoDI	GTCTC	3 cut(s)	239, 561, 808
BcuI	ACTAGT	1 cut(s)	1180
BfaI	CTAG	2 cut(s)	261, 1181
BfmI	CTRYAG	2 cut(s)	551, 1020
BfoI	RGCGCY	1 cut(s)	1252
BfuAI	ACCTGC	1 cut(s)	466
BisI	GCNGC	7 cut(s)	48, 191, 206, 220, 237, 1156, 1285
BlsI	GCNGC	7 cut(s)	49, 192, 207, 221, 238, 1157, 1286
Bme1390I	CCNGG	4 cut(s)	187, 357, 398, 511
BmeT110I	CYCGRG	1 cut(s)	290
BmgBI	CACGTC	1 cut(s)	1066
BmiI	GGNNCC	4 cut(s)	508, 538, 613, 1250
BmrFI	CCNGG	4 cut(s)	187, 357, 398, 511
BmsI	GCATC	5 cut(s)	58, 86, 625, 1216, 1296
BpiI	GAAGAC	2 cut(s)	14, 60
Bpu10I	CCTNAGC	1 cut(s)	266
BpuEI	CTTGAG	1 cut(s)	1238
BpuMI	CCSGG	1 cut(s)	187
BsaHI	GRCGYC	4 cut(s)	5, 148, 1044, 1249
BsaJI	CCNNGG	5 cut(s)	45, 185, 240, 289, 1012
BsaWI	WCCGGW	1 cut(s)	303
Bsc4I	CCNNNNNNNGG	4 cut(s)	186, 189, 814, 1151
Bse118I	RCCGGY	2 cut(s)	303, 481
Bse1I	ACTGG	2 cut(s)	505, 1047
BseBI	CCWGG	3 cut(s)	357, 398, 511
BseDI	CCNNGG	5 cut(s)	45, 185, 240, 289, 1012
BseGI	GGATG	1 cut(s)	315
BseLI	CCNNNNNNNGG	4 cut(s)	186, 189, 814, 1151
BseMII	CTCAG	2 cut(s)	280, 656
BseNI	ACTGG	2 cut(s)	505, 1047
BseXI	GCAGC	3 cut(s)	217, 223, 1271
Bsh1236I	CGCG	2 cut(s)	47, 222
Bsh1285I	CGRYCG	1 cut(s)	19
BshFI	GGCC	1 cut(s)	231
BshNI	GGYRCC	2 cut(s)	611, 1248
BshTI	ACCGGT	1 cut(s)	303
BsiEI	CGRYCG	1 cut(s)	19
BsiHKAI	GWGCWC	2 cut(s)	748, 1266
BsiHKCI	CYCGRG	1 cut(s)	290
BsiSI	CCGG	4 cut(s)	28, 187, 304, 482
BslFI	GGGAC	1 cut(s)	726
BslI	CCNNNNNNNGG	4 cut(s)	186, 189, 814, 1151
BsmAI	GTCTC	3 cut(s)	239, 561, 808
BsmBI	CGTCTC	1 cut(s)	808
BsmFI	GGGAC	1 cut(s)	726
BsmI	GAATGC	1 cut(s)	982
BsnI	GGCC	1 cut(s)	231
BsoBI	CYCGRG	1 cut(s)	290
Bsp1286I	GDGCHC	2 cut(s)	748, 1266
Bsp143I	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
Bsp19I	CCATGG	1 cut(s)	1012
BspANI	GGCC	1 cut(s)	231
BspCNI	CTCAG	2 cut(s)	279, 655
BspFNI	CGCG	2 cut(s)	47, 222
BspLI	GGNNCC	4 cut(s)	508, 538, 613, 1250
BspMAI	CTGCAG	2 cut(s)	555, 1024
BspMI	ACCTGC	1 cut(s)	466
BspPI	GGATC	5 cut(s)	453, 656, 707, 762, 1136
BspT107I	GGYRCC	2 cut(s)	611, 1248
BsrFI	RCCGGY	2 cut(s)	303, 481
BsrI	ACTGG	2 cut(s)	505, 1047
BssAI	RCCGGY	2 cut(s)	303, 481
BssECI	CCNNGG	5 cut(s)	45, 185, 240, 289, 1012
BssMI	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
BssNI	GRCGYC	4 cut(s)	5, 148, 1044, 1249
BssT1I	CCWWGG	2 cut(s)	240, 1012
Bst2UI	CCWGG	3 cut(s)	357, 398, 511
BstACI	GRCGYC	4 cut(s)	5, 148, 1044, 1249
BstC8I	GCNNGC	5 cut(s)	155, 159, 324, 583, 1282
BstDEI	CTNAG	4 cut(s)	266, 642, 774, 1148
BstDSI	CCRYGG	2 cut(s)	45, 1012
BstEII	GGTNACC	1 cut(s)	686
BstENI	CCTNNNNNAGG	1 cut(s)	1149
BstF5I	GGATG	1 cut(s)	315
BstFNI	CGCG	2 cut(s)	47, 222
BstH2I	RGCGCY	1 cut(s)	1252
BstHHI	GCGC	2 cut(s)	730, 1251
BstKTI	GATC	8 cut(s)	19, 427, 448, 651, 702, 757, 865, 1144
BstMAI	GTCTC	3 cut(s)	239, 561, 808
BstMBI	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
BstMCI	CGRYCG	1 cut(s)	19
BstMWI	GCNNNNNNNGC	5 cut(s)	68, 154, 179, 228, 1290
BstNI	CCWGG	3 cut(s)	357, 398, 511
BstNSI	RCATGY	2 cut(s)	585, 1199
BstPI	GGTNACC	1 cut(s)	686
BstSCI	CCNGG	4 cut(s)	185, 355, 396, 509
BstSFI	CTRYAG	2 cut(s)	551, 1020
BstUI	CGCG	2 cut(s)	47, 222
BstV1I	GCAGC	3 cut(s)	217, 223, 1271
BstV2I	GAAGAC	2 cut(s)	14, 60
BstX2I	RGATCY	4 cut(s)	445, 648, 699, 1141
BstXI	CCANNNNNNTGG	1 cut(s)	878
BstYI	RGATCY	4 cut(s)	445, 648, 699, 1141
BsuRI	GGCC	1 cut(s)	231
BtgI	CCRYGG	2 cut(s)	45, 1012
BtrI	CACGTC	1 cut(s)	1066
BtsCI	GGATG	1 cut(s)	315
BtsIMutI	CAGTG	1 cut(s)	625
BveI	ACCTGC	1 cut(s)	466
Cac8I	GCNNGC	5 cut(s)	155, 159, 324, 583, 1282
CfoI	GCGC	2 cut(s)	730, 1251
Cfr10I	RCCGGY	2 cut(s)	303, 481
Cfr42I	CCGCGG	1 cut(s)	48
CseI	GACGC	4 cut(s)	83, 137, 790, 1052
Csp6I	GTAC	2 cut(s)	612, 841
CspAI	ACCGGT	1 cut(s)	303
CviAII	CATG	4 cut(s)	58, 582, 1013, 1196
CviQI	GTAC	2 cut(s)	612, 841
DdeI	CTNAG	4 cut(s)	266, 642, 774, 1148
DinI	GGCGCC	1 cut(s)	1250
DpnI	GATC	8 cut(s)	18, 426, 447, 650, 701, 756, 864, 1143
DpnII	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
EaeI	YGGCCR	1 cut(s)	229
EciI	GGCGGA	2 cut(s)	65, 77
Ecl136II	GAGCTC	1 cut(s)	1264
Eco130I	CCWWGG	2 cut(s)	240, 1012
Eco24I	GRGCYC	1 cut(s)	1266
Eco53kI	GAGCTC	1 cut(s)	1264
Eco57I	CTGAAG	2 cut(s)	966, 1055
Eco88I	CYCGRG	1 cut(s)	290
Eco91I	GGTNACC	1 cut(s)	686
EcoICRI	GAGCTC	1 cut(s)	1264
EcoNI	CCTNNNNNAGG	1 cut(s)	1149
EcoO65I	GGTNACC	1 cut(s)	686
EcoRII	CCWGG	3 cut(s)	355, 396, 509
EcoT14I	CCWWGG	2 cut(s)	240, 1012
EcoT38I	GRGCYC	1 cut(s)	1266
EgeI	GGCGCC	1 cut(s)	1250
EheI	GGCGCC	1 cut(s)	1250
ErhI	CCWWGG	2 cut(s)	240, 1012
Esp3I	CGTCTC	1 cut(s)	808
FaeI	CATG	4 cut(s)	61, 585, 1016, 1199
FalI	AAGNNNNNCTT	2 cut(s)	778, 810
FaqI	GGGAC	1 cut(s)	726
FatI	CATG	4 cut(s)	57, 581, 1012, 1195
FauI	CCCGC	1 cut(s)	830
FauNDI	CATATG	1 cut(s)	655
FbaI	TGATCA	1 cut(s)	862
Fnu4HI	GCNGC	7 cut(s)	48, 191, 206, 220, 237, 1156, 1285
FokI	GGATG	1 cut(s)	322
FriOI	GRGCYC	1 cut(s)	1266
Fsp4HI	GCNGC	7 cut(s)	48, 191, 206, 220, 237, 1156, 1285
FspAI	RTGCGCAY	1 cut(s)	729
FspBI	CTAG	2 cut(s)	261, 1181
FspI	TGCGCA	1 cut(s)	729
GlaI	GCGC	2 cut(s)	729, 1250
GluI	GCNGC	7 cut(s)	48, 191, 206, 220, 237, 1156, 1285
HaeII	RGCGCY	1 cut(s)	1252
HaeIII	GGCC	1 cut(s)	231
HapII	CCGG	4 cut(s)	28, 187, 304, 482
HgaI	GACGC	4 cut(s)	83, 137, 790, 1052
HhaI	GCGC	2 cut(s)	730, 1251
Hin1I	GRCGYC	4 cut(s)	5, 148, 1044, 1249
Hin1II	CATG	4 cut(s)	61, 585, 1016, 1199
Hin6I	GCGC	2 cut(s)	728, 1249
HinP1I	GCGC	2 cut(s)	728, 1249
HindIII	AAGCTT	1 cut(s)	528
HinfI	GANTC	3 cut(s)	196, 923, 1117
HpaII	CCGG	4 cut(s)	28, 187, 304, 482
HphI	GGTGA	4 cut(s)	640, 698, 929, 1214
Hpy166II	GTNNAC	3 cut(s)	881, 958, 1063
Hpy188I	TCNGA	4 cut(s)	10, 1042, 1074, 1242
Hpy188III	TCNNGA	4 cut(s)	944, 1139, 1217, 1259
Hpy8I	GTNNAC	3 cut(s)	881, 958, 1063
Hpy99I	CGWCG	4 cut(s)	10, 77, 153, 204
HpyAV	CCTTC	6 cut(s)	97, 290, 772, 908, 1069, 1239
HpyCH4IV	ACGT	1 cut(s)	1065
HpyCH4V	TGCA	7 cut(s)	374, 461, 553, 663, 1022, 1235, 1287
HpyF10VI	GCNNNNNNNGC	5 cut(s)	68, 154, 179, 228, 1290
HpyF3I	CTNAG	4 cut(s)	266, 642, 774, 1148
HpySE526I	ACGT	1 cut(s)	1065
Hsp92I	GRCGYC	4 cut(s)	5, 148, 1044, 1249
Hsp92II	CATG	4 cut(s)	61, 585, 1016, 1199
HspAI	GCGC	2 cut(s)	728, 1249
KasI	GGCGCC	1 cut(s)	1248
KpnI	GGTACC	1 cut(s)	615
Ksp22I	TGATCA	1 cut(s)	862
KspI	CCGCGG	1 cut(s)	48
Kzo9I	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
LmnI	GCTCC	4 cut(s)	282, 506, 798, 1082
Lsp1109I	GCAGC	3 cut(s)	217, 223, 1271
LweI	GCATC	5 cut(s)	58, 86, 625, 1216, 1296
MaeI	CTAG	2 cut(s)	261, 1181
MaeII	ACGT	1 cut(s)	1065
MaeIII	GTNAC	3 cut(s)	345, 496, 686
MalI	GATC	8 cut(s)	18, 426, 447, 650, 701, 756, 864, 1143
MboI	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
MboII	GAAGA	8 cut(s)	14, 27, 65, 77, 356, 794, 932, 1127
MflI	RGATCY	4 cut(s)	445, 648, 699, 1141
MhlI	GDGCHC	2 cut(s)	748, 1266
MluCI	AATT	4 cut(s)	167, 844, 853, 1166
Mly113I	GGCGCC	1 cut(s)	1249
MlyI	GAGTC	2 cut(s)	190, 1111
MmeI	TCCRAC	1 cut(s)	423
MnlI	CCTC	9 cut(s)	138, 184, 275, 299, 677, 1005, 1145, 1155, 1248
MroXI	GAANNNNTTC	1 cut(s)	86
MseI	TTAA	3 cut(s)	852, 951, 1097
MslI	CAYNNNNRTG	3 cut(s)	575, 876, 1200
MspA1I	CMGCKG	1 cut(s)	47
MspI	CCGG	4 cut(s)	28, 187, 304, 482
MspR9I	CCNGG	4 cut(s)	187, 357, 398, 511
Mva1269I	GAATGC	1 cut(s)	982
MvaI	CCWGG	3 cut(s)	357, 398, 511
MvnI	CGCG	2 cut(s)	47, 222
MwoI	GCNNNNNNNGC	5 cut(s)	68, 154, 179, 228, 1290
NarI	GGCGCC	1 cut(s)	1249
NciI	CCSGG	1 cut(s)	187
NcoI	CCATGG	1 cut(s)	1012
NdeI	CATATG	1 cut(s)	655
NdeII	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
NlaIII	CATG	4 cut(s)	61, 585, 1016, 1199
NlaIV	GGNNCC	4 cut(s)	508, 538, 613, 1250
NmeAIII	GCCGAG	1 cut(s)	187
NmuCI	GTSAC	1 cut(s)	686
NsbI	TGCGCA	1 cut(s)	729
NspI	RCATGY	2 cut(s)	585, 1199
PaeI	GCATGC	1 cut(s)	585
PctI	GAATGC	1 cut(s)	982
PdmI	GAANNNNTTC	1 cut(s)	86
PfeI	GAWTC	1 cut(s)	923
PinAI	ACCGGT	1 cut(s)	303
PkrI	GCNGC	7 cut(s)	49, 192, 207, 221, 238, 1157, 1286
Ple19I	CGATCG	1 cut(s)	19
PleI	GAGTC	2 cut(s)	190, 1111
PluTI	GGCGCC	1 cut(s)	1252
PpsI	GAGTC	2 cut(s)	190, 1111
Psp124BI	GAGCTC	1 cut(s)	1266
Psp6I	CCWGG	3 cut(s)	355, 396, 509
PspEI	GGTNACC	1 cut(s)	686
PspGI	CCWGG	3 cut(s)	355, 396, 509
PspN4I	GGNNCC	4 cut(s)	508, 538, 613, 1250
PstI	CTGCAG	2 cut(s)	555, 1024
PsuI	RGATCY	4 cut(s)	445, 648, 699, 1141
PvuI	CGATCG	1 cut(s)	19
RsaI	GTAC	2 cut(s)	613, 842
RsaNI	GTAC	2 cut(s)	612, 841
RseI	CAYNNNNRTG	3 cut(s)	575, 876, 1200
SacI	GAGCTC	1 cut(s)	1266
SacII	CCGCGG	1 cut(s)	48
SaqAI	TTAA	3 cut(s)	852, 951, 1097
SatI	GCNGC	7 cut(s)	48, 191, 206, 220, 237, 1156, 1285
Sau3AI	GATC	8 cut(s)	16, 424, 445, 648, 699, 754, 862, 1141
SchI	GAGTC	2 cut(s)	190, 1111
ScrFI	CCNGG	4 cut(s)	187, 357, 398, 511
SduI	GDGCHC	2 cut(s)	748, 1266
SfaNI	GCATC	5 cut(s)	58, 86, 625, 1216, 1296
SfcI	CTRYAG	2 cut(s)	551, 1020
SfoI	GGCGCC	1 cut(s)	1250
Sfr303I	CCGCGG	1 cut(s)	48
SgrBI	CCGCGG	1 cut(s)	48
SmiMI	CAYNNNNRTG	3 cut(s)	575, 876, 1200
SmlI	CTYRAG	1 cut(s)	1217
SmoI	CTYRAG	1 cut(s)	1217
SpeI	ACTAGT	1 cut(s)	1180
SphI	GCATGC	1 cut(s)	585
Sse9I	AATT	4 cut(s)	167, 844, 853, 1166
SspDI	GGCGCC	1 cut(s)	1248
SspMI	CTAG	2 cut(s)	261, 1181
SstI	GAGCTC	1 cut(s)	1266
StyD4I	CCNGG	4 cut(s)	185, 355, 396, 509
StyI	CCWWGG	2 cut(s)	240, 1012
TaiI	ACGT	1 cut(s)	1068
TaqI	TCGA	4 cut(s)	80, 134, 283, 1031
TasI	AATT	4 cut(s)	167, 844, 853, 1166
TauI	GCSGC	4 cut(s)	50, 193, 222, 1158
TfiI	GAWTC	1 cut(s)	923
Tru1I	TTAA	3 cut(s)	852, 951, 1097
Tru9I	TTAA	3 cut(s)	852, 951, 1097
TscAI	CASTG	1 cut(s)	625
TseFI	GTSAC	1 cut(s)	686
TseI	GCWGC	3 cut(s)	205, 236, 1284
Tsp45I	GTSAC	1 cut(s)	686
TspDTI	ATGAA	1 cut(s)	1179
TspGWI	ACGGA	1 cut(s)	1177
TspRI	CASTG	1 cut(s)	625
XagI	CCTNNNNNAGG	1 cut(s)	1149
XapI	RAATTY	1 cut(s)	167
XceI	RCATGY	2 cut(s)	585, 1199
XcmI	CCANNNNNNNNNTGG	1 cut(s)	622
XmnI	GAANNNNTTC	1 cut(s)	86
XspI	CTAG	2 cut(s)	261, 1181

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.