MD09G1103000.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015674
Physical Location & Seq
Reverse (-)
7539020 .. 7540351
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1103000.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGAAAACTGAAGTTAGAGGAAGCATTTCCTCCATTTCTCTCCAAAATCCAAGTACTGCTGTCTTCACCACCTCTCAGGGAAAGTCTCTCACCGGCGCGCTTACAGGGTGTCTTGGAAGCCTCGACGGGGCATGTATTGAGAAGCTACTACTTCACTGTGCAAGTGCCCTGGAGAGCAATGATGTGACCTTGGCTCAACAAGTGATGTGGGTGCTCAACAATGTTGCCTCTTCTGTTGGTGACCCTAACCAAAGGCTCACTTCATGGATCTTGAGGGCACTGATTTCAAGAGCCTCCAAGGTTTTCCCTACACCTATGAATTTTAATGGAAGCAGTAGTACTAGTACCATTCCAAGTAGGTTGATGTCGGTGACTGAGCTAGCCGGGTATGTTGATCTAATCCCATGGCACCGATTCGGATATTGTGCATCAAATAGTGCAATTTTAAAGGCAATTGAAGGGTGCCCAAAAGTTCATATCTTAGATTTTAGCATCACTCATGGTGTGCAATGGCCTACTTTAATAGATGCATTGGAAAAAAGGCCTGAGGGTCCTCCTTTGCTTAGAATCACTGTTCCTAATTGGAGGCCACAAGTCCCACCTTCGCTCAATGTCTCAAGCGAAGAAGTTGGTCTTCGTTTGGGGAATTTCTCTAGGTTTAGAGATGTTCCTTTTGAGTTCAATGTGATTGAAAACTCATCCTCTTTGGAATTGCTCCTGAGCACTCAATTAAACCCTTCCTCACTAGGCCTCAGGGAAGATGAGGTTTTGGTTGTAAATTGTCAAAACTGGTTACGCTATCTTTCCGATGAGCCGCTTGGTGGTATCCCCGGCTATCAAGATGGTTCGATGCGCAACACTTTCCTTAACACGATTAGATCCCTTAACCCTAGAATCATAGTTGTGGTAGATGAAGATTCTGATCTTACCGCACCAAGTCTTAGCTCAAGAATAACAACTTGTTTTAATTATCTTTGGATACCCTTTGATTCCTTGGAAACATTCTTGCCTAAAGATAGTACTCAAAGGATGAATTATGAATCTGATGTTGGTCACAAAATTGAAAACATTATTGGTTTTGAAGGGCAACAAAGAATAGAGAGGTTGGAGTCAGGTGTCACAATGTCACAAAGATTGAGAAACATTGGTTTTTCAAGTGCTCCATTTTGTGAAGAGACGGCTAAGGAAGTGAAGGCCTTATTAGATGAACATGCAAGTGGTTGGGGCATGAAACGTGAAGAAGATATGTTGGTGCTCACATGGAAGGGACACAACTCAGTTTTTGCGACAGCTTGGGTCCCAAATCCAAATGAGTTGCTAATTGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

444

Amino Acids

49.06

Weight (kDa)

5.2

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 47 - 432 7.3e-90 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015674)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g43780
malus_domestica MD09G1103000.v1.1 MD17G1090800.v1.1
prunus_persica Prupe.3G222800_v2.0.a1
pyrus_communis pycom09g02620 pycom17g08760
rosa_chinensis RchiOBHm_Chr2g0160531
rosa_laevigata RLG00000021220
rosa_multiflora Rmu_sc0000674.1_g000006
rosa_roxburghii Rroxscaffold_2G00089740
rosa_rugosa Rorug02G0483600
rosa_samantha Rh2AG548900 Rh2BG562600 Rh2CG533200 Rh2DG571900
rosa_wichuraiana Rw2G045440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 854
AccB1I GGYRCC 2 cut(s) 408, 462
AccII CGCG 1 cut(s) 98
AciI CCGC 2 cut(s) 815, 930
AclWI GGATC 2 cut(s) 275, 873
AcsI RAATTY 2 cut(s) 319, 646
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 4 cut(s) 55, 340, 346, 1021
AfiI CCNNNNNNNGG 2 cut(s) 127, 821
AgsI TTSAA 7 cut(s) 288, 458, 682, 692, 1064, 1082, 1155
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 1 cut(s) 168
AjuI GAANNNNNNNTTGG 4 cut(s) 1032, 1056, 1064, 1088
AluBI AGCT 4 cut(s) 145, 379, 945, 1292
AluI AGCT 4 cut(s) 145, 379, 945, 1292
Alw21I GWGCWC 4 cut(s) 216, 725, 1162, 1257
Alw26I GTCTC 3 cut(s) 90, 619, 1169
AlwI GGATC 2 cut(s) 275, 873
AoxI GGCC 5 cut(s) 512, 542, 587, 748, 1194
ApoI RAATTY 2 cut(s) 319, 646
Asp700I GAANNNNTTC 1 cut(s) 25
AspLEI GCGC 3 cut(s) 98, 100, 855
AspS9I GGNCC 2 cut(s) 551, 1297
AsuC2I CCSGG 2 cut(s) 385, 831
AsuHPI GGTGA 4 cut(s) 58, 82, 251, 382
AsuNHI GCTAGC 1 cut(s) 379
AvaII GGWCC 2 cut(s) 551, 1297
AxyI CCTNAGG 2 cut(s) 546, 752
BaeGI GKGCMC 3 cut(s) 169, 280, 467
BanI GGYRCC 2 cut(s) 408, 462
BarI GAAGNNNNNNTAC 2 cut(s) 322, 354
BbsI GAAGAC 2 cut(s) 55, 626
Bbv12I GWGCWC 4 cut(s) 216, 725, 1162, 1257
BccI CCATC 1 cut(s) 836
BceAI ACGGC 1 cut(s) 1194
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 2 cut(s) 836, 972
BcnI CCSGG 2 cut(s) 385, 831
BcoDI GTCTC 3 cut(s) 90, 619, 1169
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 5 cut(s) 342, 380, 654, 746, 891
BfuI GTATCC 2 cut(s) 836, 972
BisI GCNGC 1 cut(s) 815
BlsI GCNGC 1 cut(s) 816
BmcAI AGTACT 3 cut(s) 55, 340, 1021
Bme1390I CCNGG 3 cut(s) 170, 385, 831
Bme18I GGWCC 2 cut(s) 551, 1297
BmgT120I GGNCC 2 cut(s) 551, 1297
BmiI GGNNCC 5 cut(s) 410, 464, 552, 1298, 1299
BmrFI CCNGG 3 cut(s) 170, 385, 831
BmsI GCATC 4 cut(s) 437, 501, 517, 840
BmtI GCTAGC 1 cut(s) 383
BpiI GAAGAC 2 cut(s) 55, 626
BpmI CTGGAG 1 cut(s) 191
Bpu10I CCTNAGC 2 cut(s) 719, 1182
BpuEI CTTGAG 3 cut(s) 292, 601, 931
BpuMI CCSGG 2 cut(s) 385, 831
BsaBI GATNNNNATC 1 cut(s) 921
BsaJI CCNNGG 6 cut(s) 168, 189, 297, 404, 829, 993
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 821
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 794
Bse21I CCTNAGG 2 cut(s) 546, 752
Bse3DI GCAATG 2 cut(s) 184, 515
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 6 cut(s) 168, 189, 297, 404, 829, 993
BseGI GGATG 2 cut(s) 698, 1035
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 2 cut(s) 127, 821
BseMI GCAATG 2 cut(s) 184, 515
BseMII CTCAG 6 cut(s) 89, 366, 537, 710, 766, 1290
BseNI ACTGG 1 cut(s) 794
BsePI GCGCGC 1 cut(s) 96
BseSI GKGCMC 3 cut(s) 169, 280, 467
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 5 cut(s) 514, 544, 589, 750, 1196
BshNI GGYRCC 2 cut(s) 408, 462
BsiHKAI GWGCWC 4 cut(s) 216, 725, 1162, 1257
BsiSI CCGG 3 cut(s) 93, 384, 831
BslFI GGGAC 3 cut(s) 581, 1281, 1283
BslI CCNNNNNNNGG 2 cut(s) 127, 821
BsmAI GTCTC 3 cut(s) 90, 619, 1169
BsmBI CGTCTC 1 cut(s) 1169
BsmFI GGGAC 3 cut(s) 581, 1281, 1283
BsnI GGCC 5 cut(s) 514, 544, 589, 750, 1196
Bsp1286I GDGCHC 7 cut(s) 169, 216, 280, 467, 725, 1162, 1257
Bsp143I GATC 4 cut(s) 267, 394, 878, 922
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 2 cut(s) 815, 930
BspANI GGCC 5 cut(s) 514, 544, 589, 750, 1196
BspCNI CTCAG 6 cut(s) 88, 367, 538, 711, 765, 1289
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 5 cut(s) 410, 464, 552, 1298, 1299
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 2 cut(s) 275, 873
BspT107I GGYRCC 2 cut(s) 408, 462
BsrDI GCAATG 2 cut(s) 184, 515
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 1 cut(s) 794
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 6 cut(s) 168, 189, 297, 404, 829, 993
BssHII GCGCGC 1 cut(s) 96
BssMI GATC 4 cut(s) 267, 394, 878, 922
BssT1I CCWWGG 4 cut(s) 189, 297, 404, 993
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 158, 574
Bst6I CTCTTC 2 cut(s) 235, 1167
BstC8I GCNNGC 2 cut(s) 98, 381
BstDSI CCRYGG 1 cut(s) 404
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 2 cut(s) 698, 1035
BstFNI CGCG 1 cut(s) 98
BstHHI GCGC 3 cut(s) 98, 100, 855
BstKTI GATC 4 cut(s) 270, 397, 881, 925
BstMAI GTCTC 3 cut(s) 90, 619, 1169
BstMBI GATC 4 cut(s) 267, 394, 878, 922
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 2 cut(s) 135, 1214
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 3 cut(s) 168, 383, 829
BstSLI GKGCMC 3 cut(s) 169, 280, 467
BstUI CGCG 1 cut(s) 98
BstV2I GAAGAC 2 cut(s) 55, 626
BstX2I RGATCY 2 cut(s) 267, 878
BstYI RGATCY 2 cut(s) 267, 878
Bsu36I CCTNAGG 2 cut(s) 546, 752
BsuI GTATCC 2 cut(s) 836, 972
BsuRI GGCC 5 cut(s) 514, 544, 589, 750, 1196
BtgI CCRYGG 1 cut(s) 404
BtsCI GGATG 2 cut(s) 698, 1035
BtsIMutI CAGTG 3 cut(s) 154, 278, 570
Cac8I GCNNGC 2 cut(s) 98, 381
CfoI GCGC 3 cut(s) 98, 100, 855
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 551, 1297
Csp6I GTAC 4 cut(s) 54, 339, 345, 1020
CspCI CAANNNNNGTGG 2 cut(s) 188, 223
CviAII CATG 7 cut(s) 132, 264, 405, 500, 1211, 1228, 1260
CviQI GTAC 4 cut(s) 54, 339, 345, 1020
DpnI GATC 4 cut(s) 269, 396, 880, 924
DpnII GATC 4 cut(s) 267, 394, 878, 922
DraI TTTAAA 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 235, 1167
EarI CTCTTC 2 cut(s) 235, 1167
Eco130I CCWWGG 4 cut(s) 189, 297, 404, 993
Eco147I AGGCCT 3 cut(s) 544, 750, 1196
Eco47I GGWCC 2 cut(s) 551, 1297
Eco57I CTGAAG 1 cut(s) 30
Eco81I CCTNAGG 2 cut(s) 546, 752
Eco91I GGTNACC 1 cut(s) 239
EcoO109I RGGNCCY 2 cut(s) 551, 1297
EcoO65I GGTNACC 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 4 cut(s) 189, 297, 404, 993
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 4 cut(s) 189, 297, 404, 993
Esp3I CGTCTC 1 cut(s) 1169
FaeI CATG 7 cut(s) 135, 267, 408, 503, 1214, 1231, 1263
FalI AAGNNNNNCTT 4 cut(s) 244, 276, 618, 650
FaqI GGGAC 3 cut(s) 581, 1281, 1283
FatI CATG 7 cut(s) 131, 263, 404, 499, 1210, 1227, 1259
Fnu4HI GCNGC 1 cut(s) 815
FokI GGATG 2 cut(s) 685, 1042
Fsp4HI GCNGC 1 cut(s) 815
FspBI CTAG 5 cut(s) 342, 380, 654, 746, 891
FspI TGCGCA 1 cut(s) 854
GlaI GCGC 3 cut(s) 97, 99, 854
GluI GCNGC 1 cut(s) 815
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 5 cut(s) 514, 544, 589, 750, 1196
HapII CCGG 3 cut(s) 93, 384, 831
HhaI GCGC 3 cut(s) 98, 100, 855
Hin1II CATG 7 cut(s) 135, 267, 408, 503, 1214, 1231, 1263
Hin6I GCGC 3 cut(s) 96, 98, 853
HinP1I GCGC 3 cut(s) 96, 98, 853
HinfI GANTC 7 cut(s) 414, 567, 894, 917, 989, 1040, 1109
HpaII CCGG 3 cut(s) 93, 384, 831
HphI GGTGA 4 cut(s) 58, 82, 251, 382
Hpy188I TCNGA 4 cut(s) 419, 808, 922, 1045
Hpy188III TCNNGA 5 cut(s) 271, 288, 718, 839, 948
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 6 cut(s) 452, 612, 747, 1076, 1186, 1258
HpyCH4III ACNGT 2 cut(s) 158, 574
HpyCH4IV ACGT 1 cut(s) 1234
HpyCH4V TGCA 6 cut(s) 161, 428, 440, 508, 530, 1214
HpySE526I ACGT 1 cut(s) 1234
Hsp92II CATG 7 cut(s) 135, 267, 408, 503, 1214, 1231, 1263
HspAI GCGC 3 cut(s) 96, 98, 853
KflI GGGWCCC 1 cut(s) 1297
Kzo9I GATC 4 cut(s) 267, 394, 878, 922
LmnI GCTCC 2 cut(s) 720, 1165
LweI GCATC 4 cut(s) 437, 501, 517, 840
MaeI CTAG 5 cut(s) 342, 380, 654, 746, 891
MaeII ACGT 1 cut(s) 1234
MaeIII GTNAC 7 cut(s) 184, 239, 370, 792, 1052, 1117, 1125
MalI GATC 4 cut(s) 269, 396, 880, 924
MboI GATC 4 cut(s) 267, 394, 878, 922
MboII GAAGA 9 cut(s) 55, 222, 626, 635, 770, 926, 1184, 1250, 1253
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 2 cut(s) 267, 878
MhlI GDGCHC 7 cut(s) 169, 216, 280, 467, 725, 1162, 1257
MlyI GAGTC 1 cut(s) 1118
MmeI TCCRAC 1 cut(s) 1086
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 8 cut(s) 324, 446, 521, 731, 867, 885, 966, 1330
MslI CAYNNNNRTG 2 cut(s) 902, 1215
MspI CCGG 3 cut(s) 93, 384, 831
MspR9I CCNGG 3 cut(s) 170, 385, 831
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 98
NciI CCSGG 2 cut(s) 385, 831
NcoI CCATGG 1 cut(s) 404
NdeII GATC 4 cut(s) 267, 394, 878, 922
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 7 cut(s) 135, 267, 408, 503, 1214, 1231, 1263
NlaIV GGNNCC 5 cut(s) 410, 464, 552, 1298, 1299
NmuCI GTSAC 6 cut(s) 184, 239, 370, 1052, 1117, 1125
NsbI TGCGCA 1 cut(s) 854
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 2 cut(s) 135, 1214
PauI GCGCGC 1 cut(s) 96
PceI AGGCCT 3 cut(s) 544, 750, 1196
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 6 cut(s) 414, 567, 894, 917, 989, 1040
PkrI GCNGC 1 cut(s) 816
PleI GAGTC 1 cut(s) 1117
PpsI GAGTC 1 cut(s) 1117
PpuMI RGGWCCY 2 cut(s) 551, 1297
Psp5II RGGWCCY 2 cut(s) 551, 1297
Psp6I CCWGG 1 cut(s) 168
PspEI GGTNACC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 5 cut(s) 410, 464, 552, 1298, 1299
PspPI GGNCC 2 cut(s) 551, 1297
PspPPI RGGWCCY 2 cut(s) 551, 1297
PsuI RGATCY 2 cut(s) 267, 878
PteI GCGCGC 1 cut(s) 96
RsaI GTAC 4 cut(s) 55, 340, 346, 1021
RsaNI GTAC 4 cut(s) 54, 339, 345, 1020
RseI CAYNNNNRTG 2 cut(s) 902, 1215
SaqAI TTAA 8 cut(s) 324, 446, 521, 731, 867, 885, 966, 1330
SatI GCNGC 1 cut(s) 815
Sau3AI GATC 4 cut(s) 267, 394, 878, 922
Sau96I GGNCC 2 cut(s) 551, 1297
ScaI AGTACT 3 cut(s) 55, 340, 1021
SchI GAGTC 1 cut(s) 1118
ScrFI CCNGG 3 cut(s) 170, 385, 831
SduI GDGCHC 7 cut(s) 169, 216, 280, 467, 725, 1162, 1257
SfaNI GCATC 4 cut(s) 437, 501, 517, 840
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 2 cut(s) 551, 1297
SmiMI CAYNNNNRTG 2 cut(s) 902, 1215
SmlI CTYRAG 3 cut(s) 271, 616, 946
SmoI CTYRAG 3 cut(s) 271, 616, 946
SpeI ACTAGT 1 cut(s) 341
SseBI AGGCCT 3 cut(s) 544, 750, 1196
SsiI CCGC 2 cut(s) 815, 930
SspMI CTAG 5 cut(s) 342, 380, 654, 746, 891
StuI AGGCCT 3 cut(s) 544, 750, 1196
StyD4I CCNGG 3 cut(s) 168, 383, 829
StyI CCWWGG 4 cut(s) 189, 297, 404, 993
TaaI ACNGT 2 cut(s) 158, 574
TaiI ACGT 1 cut(s) 1237
TaqI TCGA 2 cut(s) 123, 848
TatI WGTACW 3 cut(s) 53, 338, 1019
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 6 cut(s) 414, 567, 894, 917, 989, 1040
Tru1I TTAA 8 cut(s) 324, 446, 521, 731, 867, 885, 966, 1330
Tru9I TTAA 8 cut(s) 324, 446, 521, 731, 867, 885, 966, 1330
TscAI CASTG 3 cut(s) 161, 285, 577
TseFI GTSAC 6 cut(s) 184, 239, 370, 1052, 1117, 1125
Tsp45I GTSAC 6 cut(s) 184, 239, 370, 1052, 1117, 1125
TspDTI ATGAA 9 cut(s) 17, 252, 332, 464, 927, 1046, 1053, 1221, 1244
TspRI CASTG 3 cut(s) 161, 285, 577
VpaK11BI GGWCC 2 cut(s) 551, 1297
XapI RAATTY 2 cut(s) 319, 646
XceI RCATGY 2 cut(s) 135, 1214
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 5 cut(s) 342, 380, 654, 746, 891
ZrmI AGTACT 3 cut(s) 55, 340, 1021
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.