MD17G1090800.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015674
Physical Location & Seq
Reverse (-)
7544673 .. 7546001
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1090800.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGAAAACTGAAGTTCGAGGAAGCATTACCTCCATTTCTCTCCAAAATCCAAGTACTGCTCTCTTCAACACCTCTCAGGGCACGTCCCTCACCGGTGCGCTTACCGGGTGTCTCGGAAGCCTCGACGGGGCATGTATTGAGAAGCTACTACTTCACTGTGCAAGTGCCCTAGAGAGCAATGATGTGACCTTGGCTCAACAAGTGATGTGGGTGCTCAACAATGTTGCCTCTTCTGTTGGTGACCCTAACCAAAGGCTCACCTCATGGATCTTGAGGGCACTAATTTCAAGAGCCTCAAAGGTTTGCCCTACACCTATGAATTTTAATGGAAGCAGTAGCACTAGTACCATTCCGAGTAGGTTGATGTCGGTGACCGAGCTAGCCGGGTATGTTGATCTAATCCCATGGCACCGATTCGGATACTGTGCATCAAATAGTGCAATTTTTAAGGCAATTCAAGGGTGCCCAAAAGTTCATATCTTAGATTTTAGCATCACTCATTGTATGCAATGGCCTACTTTAATAGATGCATTGGCCAAAAGGCCTGAGGGTCCTCCTTTGCTTAGAGTCTCTGTGCCTAATTGGAGGCCACAAGTCCCTCCTTTGCTCAATGTTTCAAGCGAAGAAGTTGGTCTTCGTTTGGGGAATTTCTCTAGGTTTAGAGATGTCCCTTTTGAGTTCAATGTGATTGAAAACTCATCCTCTTTGGAATTGCTAGTTACCTCTGAATTAAACCCTTCCTCACTAGACCTCCGGGAAGATGAGGTTTTGGTTGTGAATTGTCAAAATTGGTTACGCTATCTTTCCGATGAGCCAGGTGGTACCCCTGGCCATCAAGATGGTTCGATGCGCAACACTTTCCTTAACACGATTAGATCCCTTAACCCTAGAATCATAGTTGTGGTAGATGAAGATTCTGATCTTAGTGCACCAAGTCTTACCTCAAGAATAACAACTTGTTTTAATTATCTTTGGATACCCTTTGATTCCTTGGATACATTCTTGCCCAAAGATAGTACCCAAAGGATGGATTATGAATCTGATGTTGGTCACAAAATTGAAAACATTATTGGTTTTGAAGGGCACAAAAGAATAGAGAGGTTGGAGTCAGGTGTCACAATGTCACAAAGATTGAGAAACATTGGTTTTTCAAGTACTATATTTTGTGAAGAGACTGCTAGGGAAGTGAAGGCCTTGCTAGATGAACATGCAAGTGGTTGGGGCATGAAACGTGAAGAAGATATGTTGGTGCTCACATGGAAGGGACACAACTCAGTTTTTGCCACAGCTTGGGTCCCAAATCCAAATGGGTTGCTAATTGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

443

Amino Acids

48.97

Weight (kDa)

5.31

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 47 - 431 2.3e-88 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015674)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g43780
malus_domestica MD09G1103000.v1.1 MD17G1090800.v1.1
prunus_persica Prupe.3G222800_v2.0.a1
pyrus_communis pycom09g02620 pycom17g08760
rosa_chinensis RchiOBHm_Chr2g0160531
rosa_laevigata RLG00000021220
rosa_multiflora Rmu_sc0000674.1_g000006
rosa_roxburghii Rroxscaffold_2G00089740
rosa_rugosa Rorug02G0483600
rosa_samantha Rh2AG548900 Rh2BG562600 Rh2CG533200 Rh2DG571900
rosa_wichuraiana Rw2G045440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 821
AccB1I GGYRCC 3 cut(s) 408, 462, 821
AccB7I CCANNNNNTGG 2 cut(s) 1027, 1290
AclWI GGATC 2 cut(s) 275, 870
AcoI YGGCCR 2 cut(s) 534, 829
AcsI RAATTY 2 cut(s) 319, 646
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 5 cut(s) 55, 346, 823, 1018, 1156
AfiI CCNNNNNNNGG 3 cut(s) 127, 1027, 1290
AgeI ACCGGT 1 cut(s) 92
AgsI TTSAA 9 cut(s) 67, 288, 458, 618, 682, 692, 1061, 1079, 1152
AhlI ACTAGT 1 cut(s) 341
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 2 cut(s) 814, 826
AjuI GAANNNNNNNTTGG 4 cut(s) 1029, 1053, 1061, 1085
AluBI AGCT 3 cut(s) 145, 379, 1289
AluI AGCT 3 cut(s) 145, 379, 1289
Alw21I GWGCWC 3 cut(s) 216, 931, 1254
Alw26I GTCTC 3 cut(s) 116, 574, 1166
Alw44I GTGCAC 1 cut(s) 927
AlwI GGATC 2 cut(s) 275, 870
AoxI GGCC 6 cut(s) 512, 534, 542, 587, 829, 1191
ApaLI GTGCAC 1 cut(s) 927
ApoI RAATTY 2 cut(s) 319, 646
AsiGI ACCGGT 1 cut(s) 92
Asp718I GGTACC 1 cut(s) 821
AspLEI GCGC 2 cut(s) 100, 852
AspS9I GGNCC 2 cut(s) 551, 1294
AsuC2I CCSGG 3 cut(s) 106, 385, 755
AsuHPI GGTGA 4 cut(s) 82, 250, 251, 382
AsuNHI GCTAGC 1 cut(s) 379
AvaII GGWCC 2 cut(s) 551, 1294
AxyI CCTNAGG 1 cut(s) 546
BaeGI GKGCMC 6 cut(s) 83, 169, 280, 467, 931, 1086
BalI TGGCCA 2 cut(s) 536, 831
BanI GGYRCC 3 cut(s) 408, 462, 821
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 3 cut(s) 216, 931, 1254
BccI CCATC 3 cut(s) 833, 840, 1021
BciT130I CCWGG 2 cut(s) 816, 828
BciVI GTATCC 3 cut(s) 413, 969, 988
BcnI CCSGG 3 cut(s) 106, 385, 755
BcoDI GTCTC 3 cut(s) 116, 574, 1166
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 9 cut(s) 170, 342, 380, 654, 716, 746, 888, 1179, 1199
BfuI GTATCC 3 cut(s) 413, 969, 988
BmcAI AGTACT 2 cut(s) 55, 1156
Bme1390I CCNGG 5 cut(s) 106, 385, 755, 816, 828
Bme18I GGWCC 2 cut(s) 551, 1294
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 2 cut(s) 551, 1294
BmiI GGNNCC 6 cut(s) 410, 464, 552, 823, 1295, 1296
BmrFI CCNGG 5 cut(s) 106, 385, 755, 816, 828
BmsI GCATC 4 cut(s) 437, 501, 517, 837
BmtI GCTAGC 1 cut(s) 383
BpiI GAAGAC 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 292, 928
BpuMI CCSGG 3 cut(s) 106, 385, 755
BsaBI GATNNNNATC 1 cut(s) 918
BsaJI CCNNGG 4 cut(s) 189, 404, 826, 990
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 735, 765
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 1027, 1290
Bse118I RCCGGY 1 cut(s) 92
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 2 cut(s) 184, 515
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 2 cut(s) 816, 828
BseDI CCNNGG 4 cut(s) 189, 404, 826, 990
BseGI GGATG 2 cut(s) 698, 1032
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 3 cut(s) 127, 1027, 1290
BseMI GCAATG 2 cut(s) 184, 515
BseMII CTCAG 3 cut(s) 89, 537, 1287
BseSI GKGCMC 6 cut(s) 83, 169, 280, 467, 931, 1086
BshFI GGCC 6 cut(s) 514, 536, 544, 589, 831, 1193
BshNI GGYRCC 3 cut(s) 408, 462, 821
BshTI ACCGGT 1 cut(s) 92
BsiHKAI GWGCWC 3 cut(s) 216, 931, 1254
BsiSI CCGG 4 cut(s) 93, 105, 384, 754
BslFI GGGAC 5 cut(s) 70, 581, 653, 1278, 1280
BslI CCNNNNNNNGG 3 cut(s) 127, 1027, 1290
BsmAI GTCTC 3 cut(s) 116, 574, 1166
BsmFI GGGAC 5 cut(s) 70, 581, 653, 1278, 1280
BsnI GGCC 6 cut(s) 514, 536, 544, 589, 831, 1193
Bsp1286I GDGCHC 8 cut(s) 83, 169, 216, 280, 467, 931, 1086, 1254
Bsp143I GATC 4 cut(s) 267, 394, 875, 919
Bsp19I CCATGG 1 cut(s) 404
BspANI GGCC 6 cut(s) 514, 536, 544, 589, 831, 1193
BspCNI CTCAG 3 cut(s) 88, 538, 1286
BspLI GGNNCC 6 cut(s) 410, 464, 552, 823, 1295, 1296
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 2 cut(s) 275, 870
BspT107I GGYRCC 3 cut(s) 408, 462, 821
BsrDI GCAATG 2 cut(s) 184, 515
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 4 cut(s) 189, 404, 826, 990
BssMI GATC 4 cut(s) 267, 394, 875, 919
BssT1I CCWWGG 3 cut(s) 189, 404, 990
Bst2UI CCWGG 2 cut(s) 816, 828
Bst4CI ACNGT 2 cut(s) 158, 425
Bst6I CTCTTC 3 cut(s) 68, 235, 1164
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 6 cut(s) 75, 481, 546, 563, 923, 1273
BstDSI CCRYGG 1 cut(s) 404
BstEII GGTNACC 2 cut(s) 239, 370
BstF5I GGATG 2 cut(s) 698, 1032
BstHHI GCGC 2 cut(s) 100, 852
BstKTI GATC 4 cut(s) 270, 397, 878, 922
BstMAI GTCTC 3 cut(s) 116, 574, 1166
BstMBI GATC 4 cut(s) 267, 394, 875, 919
BstNI CCWGG 2 cut(s) 816, 828
BstNSI RCATGY 2 cut(s) 135, 1211
BstPI GGTNACC 2 cut(s) 239, 370
BstSCI CCNGG 5 cut(s) 104, 383, 753, 814, 826
BstSLI GKGCMC 6 cut(s) 83, 169, 280, 467, 931, 1086
BstV2I GAAGAC 1 cut(s) 626
BstX2I RGATCY 2 cut(s) 267, 875
BstXI CCANNNNNNTGG 1 cut(s) 839
BstYI RGATCY 2 cut(s) 267, 875
Bsu36I CCTNAGG 1 cut(s) 546
BsuI GTATCC 3 cut(s) 413, 969, 988
BsuRI GGCC 6 cut(s) 514, 536, 544, 589, 831, 1193
BtgI CCRYGG 1 cut(s) 404
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 2 cut(s) 698, 1032
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 2 cut(s) 100, 852
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 551, 1294
Csp6I GTAC 5 cut(s) 54, 345, 822, 1017, 1155
CspAI ACCGGT 1 cut(s) 92
CspCI CAANNNNNGTGG 2 cut(s) 188, 223
CviAII CATG 6 cut(s) 132, 264, 405, 1208, 1225, 1257
CviQI GTAC 5 cut(s) 54, 345, 822, 1017, 1155
DdeI CTNAG 6 cut(s) 75, 481, 546, 563, 923, 1273
DpnI GATC 4 cut(s) 269, 396, 877, 921
DpnII GATC 4 cut(s) 267, 394, 875, 919
EaeI YGGCCR 2 cut(s) 534, 829
Eam1104I CTCTTC 3 cut(s) 68, 235, 1164
EarI CTCTTC 3 cut(s) 68, 235, 1164
Eco130I CCWWGG 3 cut(s) 189, 404, 990
Eco147I AGGCCT 2 cut(s) 544, 1193
Eco47I GGWCC 2 cut(s) 551, 1294
Eco57I CTGAAG 1 cut(s) 30
Eco81I CCTNAGG 1 cut(s) 546
Eco91I GGTNACC 2 cut(s) 239, 370
EcoO109I RGGNCCY 2 cut(s) 551, 1294
EcoO65I GGTNACC 2 cut(s) 239, 370
EcoRII CCWGG 2 cut(s) 814, 826
EcoT14I CCWWGG 3 cut(s) 189, 404, 990
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 3 cut(s) 189, 404, 990
FaeI CATG 6 cut(s) 135, 267, 408, 1211, 1228, 1260
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 5 cut(s) 70, 581, 653, 1278, 1280
FatI CATG 6 cut(s) 131, 263, 404, 1207, 1224, 1256
FokI GGATG 2 cut(s) 685, 1039
FspBI CTAG 9 cut(s) 170, 342, 380, 654, 716, 746, 888, 1179, 1199
FspI TGCGCA 1 cut(s) 851
GlaI GCGC 2 cut(s) 99, 851
HaeIII GGCC 6 cut(s) 514, 536, 544, 589, 831, 1193
HapII CCGG 4 cut(s) 93, 105, 384, 754
HhaI GCGC 2 cut(s) 100, 852
Hin1II CATG 6 cut(s) 135, 267, 408, 1211, 1228, 1260
Hin6I GCGC 2 cut(s) 98, 850
HinP1I GCGC 2 cut(s) 98, 850
HinfI GANTC 7 cut(s) 414, 567, 891, 914, 986, 1037, 1106
HpaII CCGG 4 cut(s) 93, 105, 384, 754
HphI GGTGA 4 cut(s) 82, 250, 251, 382
Hpy166II GTNNAC 1 cut(s) 929
Hpy188I TCNGA 7 cut(s) 116, 354, 419, 727, 808, 919, 1042
Hpy188III TCNNGA 4 cut(s) 271, 288, 836, 945
Hpy8I GTNNAC 1 cut(s) 929
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 4 cut(s) 747, 1073, 1183, 1255
HpyCH4III ACNGT 2 cut(s) 158, 425
HpyCH4IV ACGT 2 cut(s) 83, 1231
HpyCH4V TGCA 7 cut(s) 161, 428, 440, 508, 530, 929, 1211
HpyF3I CTNAG 6 cut(s) 75, 481, 546, 563, 923, 1273
HpySE526I ACGT 2 cut(s) 83, 1231
Hsp92II CATG 6 cut(s) 135, 267, 408, 1211, 1228, 1260
HspAI GCGC 2 cut(s) 98, 850
KflI GGGWCCC 1 cut(s) 1294
KpnI GGTACC 1 cut(s) 825
Kzo9I GATC 4 cut(s) 267, 394, 875, 919
LweI GCATC 4 cut(s) 437, 501, 517, 837
MaeI CTAG 9 cut(s) 170, 342, 380, 654, 716, 746, 888, 1179, 1199
MaeII ACGT 2 cut(s) 83, 1231
MaeIII GTNAC 8 cut(s) 184, 239, 370, 718, 792, 1049, 1114, 1122
MalI GATC 4 cut(s) 269, 396, 877, 921
MboI GATC 4 cut(s) 267, 394, 875, 919
MboII GAAGA 9 cut(s) 55, 222, 626, 635, 770, 923, 1181, 1247, 1250
MflI RGATCY 2 cut(s) 267, 875
MhlI GDGCHC 8 cut(s) 83, 169, 216, 280, 467, 931, 1086, 1254
MlsI TGGCCA 2 cut(s) 536, 831
MluNI TGGCCA 2 cut(s) 536, 831
MlyI GAGTC 2 cut(s) 576, 1115
MmeI TCCRAC 1 cut(s) 1083
Mox20I TGGCCA 2 cut(s) 536, 831
Mph1103I ATGCAT 1 cut(s) 532
MscI TGGCCA 2 cut(s) 536, 831
MseI TTAA 8 cut(s) 324, 447, 521, 731, 864, 882, 963, 1327
MslI CAYNNNNRTG 3 cut(s) 837, 899, 1212
Msp20I TGGCCA 2 cut(s) 536, 831
MspI CCGG 4 cut(s) 93, 105, 384, 754
MspR9I CCNGG 5 cut(s) 106, 385, 755, 816, 828
MvaI CCWGG 2 cut(s) 816, 828
NciI CCSGG 3 cut(s) 106, 385, 755
NcoI CCATGG 1 cut(s) 404
NdeII GATC 4 cut(s) 267, 394, 875, 919
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 6 cut(s) 135, 267, 408, 1211, 1228, 1260
NlaIV GGNNCC 6 cut(s) 410, 464, 552, 823, 1295, 1296
NmuCI GTSAC 6 cut(s) 184, 239, 370, 1049, 1114, 1122
NsbI TGCGCA 1 cut(s) 851
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 2 cut(s) 135, 1211
PceI AGGCCT 2 cut(s) 544, 1193
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 5 cut(s) 414, 891, 914, 986, 1037
PflMI CCANNNNNTGG 2 cut(s) 1027, 1290
PfoI TCCNGGA 1 cut(s) 753
PinAI ACCGGT 1 cut(s) 92
PleI GAGTC 2 cut(s) 575, 1114
PpsI GAGTC 2 cut(s) 575, 1114
PpuMI RGGWCCY 2 cut(s) 551, 1294
Psp5II RGGWCCY 2 cut(s) 551, 1294
Psp6I CCWGG 2 cut(s) 814, 826
PspEI GGTNACC 2 cut(s) 239, 370
PspGI CCWGG 2 cut(s) 814, 826
PspN4I GGNNCC 6 cut(s) 410, 464, 552, 823, 1295, 1296
PspPI GGNCC 2 cut(s) 551, 1294
PspPPI RGGWCCY 2 cut(s) 551, 1294
PsuI RGATCY 2 cut(s) 267, 875
RsaI GTAC 5 cut(s) 55, 346, 823, 1018, 1156
RsaNI GTAC 5 cut(s) 54, 345, 822, 1017, 1155
RseI CAYNNNNRTG 3 cut(s) 837, 899, 1212
SaqAI TTAA 8 cut(s) 324, 447, 521, 731, 864, 882, 963, 1327
Sau3AI GATC 4 cut(s) 267, 394, 875, 919
Sau96I GGNCC 2 cut(s) 551, 1294
ScaI AGTACT 2 cut(s) 55, 1156
SchI GAGTC 2 cut(s) 576, 1115
ScrFI CCNGG 5 cut(s) 106, 385, 755, 816, 828
SduI GDGCHC 8 cut(s) 83, 169, 216, 280, 467, 931, 1086, 1254
SfaNI GCATC 4 cut(s) 437, 501, 517, 837
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 2 cut(s) 551, 1294
SmiMI CAYNNNNRTG 3 cut(s) 837, 899, 1212
SmlI CTYRAG 2 cut(s) 271, 943
SmoI CTYRAG 2 cut(s) 271, 943
SpeI ACTAGT 1 cut(s) 341
SseBI AGGCCT 2 cut(s) 544, 1193
SspMI CTAG 9 cut(s) 170, 342, 380, 654, 716, 746, 888, 1179, 1199
StuI AGGCCT 2 cut(s) 544, 1193
StyD4I CCNGG 5 cut(s) 104, 383, 753, 814, 826
StyI CCWWGG 3 cut(s) 189, 404, 990
TaaI ACNGT 2 cut(s) 158, 425
TaiI ACGT 2 cut(s) 86, 1234
TaqI TCGA 3 cut(s) 16, 123, 845
TaqII GACCGA 1 cut(s) 389
TatI WGTACW 2 cut(s) 53, 1154
TfiI GAWTC 5 cut(s) 414, 891, 914, 986, 1037
Tru1I TTAA 8 cut(s) 324, 447, 521, 731, 864, 882, 963, 1327
Tru9I TTAA 8 cut(s) 324, 447, 521, 731, 864, 882, 963, 1327
TscAI CASTG 1 cut(s) 161
TseFI GTSAC 6 cut(s) 184, 239, 370, 1049, 1114, 1122
Tsp45I GTSAC 6 cut(s) 184, 239, 370, 1049, 1114, 1122
TspDTI ATGAA 7 cut(s) 17, 332, 464, 924, 1050, 1218, 1241
TspRI CASTG 1 cut(s) 161
Van91I CCANNNNNTGG 2 cut(s) 1027, 1290
VneI GTGCAC 1 cut(s) 927
VpaK11BI GGWCC 2 cut(s) 551, 1294
XapI RAATTY 2 cut(s) 319, 646
XceI RCATGY 2 cut(s) 135, 1211
XspI CTAG 9 cut(s) 170, 342, 380, 654, 716, 746, 888, 1179, 1199
ZrmI AGTACT 2 cut(s) 55, 1156
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.