RLG00000021220

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015674
Physical Location & Seq
Forward (+)
73060538 .. 73061881
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021220

Sequence Viewer

Length: 1344 bp
ATGAAAACTGAAGTGAGAGTAGGAAGCAGTACGTCAATTTCTGCTTTACAGAATCCGAGTCTCTTGATCAACAGCACCACCTCTCAGGGCTCCCTTACCGGTGCCCTCAGAGGGTGCCTCGGAAGCCTCGATGGGGCTTGCATAGAGAAGCTATTGCTTCACTGCACGAGTGCCCTAGAGAGCAACGACGTCACTTTAGCTCAGCAAGTGATGTGGGTGCTCAACAATGTCGCTTCTTCTGTTGGTGACCCTAACCAAAGGCTCACCTCCTGGATCTTAAGGGCACTGATCTCAAGATCCTCTAGAATTTGCCCGAGTCTCAACAATAACGGAGGCGGCGGACCTAACCCTAGTACTAGTACTACTAGCACCATCGCGGCGGAGAGGAGGCTGATGACGGTGACGGAGCTAGCTGGGTACGTTGATCTAATCCCATGGCACAGGTTTGGATATTGTGCATCCAACAGTGCAATTTTCCAAGCAATTCAAGGGTGCTCAAAAATTCATATCTTGGACTTTAGCATCACGCACTGTATGCAGTGGCCTACTCTCATAGATGCTCTGGGGAAAAGACCAGAAGGCCCTCCTGATTCACTTAGAGTGACTGTTCCCTCTTGTAGGCCTCTAGTCCCTCCTTTGCTCAATGTGTCAATTGAAGAAGTCGGTCTTCGTTTGGGAAATTTCTCTAGGTTTAGGGATGTCCCATTTGAGTTCAATGTCATCGAGAACTCCTCTTCTTTCGAGTTGTTATTGAGCCAATTAAACCCTACTTCGTTAAATCTCAAGGATGACGAGGCCTTGGTGGTAAATTGCCAAAATTGGTTACGCTATTTGTCCGATGATGAGGGTAGAACTAATAGTGCTCGTAACACTTTTCTTAACATGATTAGATGTCTCAACCCTAGAATCATAGTCGTAGTGGACGAAGATTCCGATCTAAGTTCCCCGAGCCTCAGTTCGAGAATCACCACATGTTTTAACTACTTGTGGATACCCTTTGATTGCTTGGAGACTTTCTTGCCCAAAGATAGTAACCAGAGGATGGAGTATGAGTCCGACATTGGTCAGAAGATCGAGAACATTATCGGTTTCGAAGGGGTCCAGAGAATAGAGAGGCTAGAGTCCGGCGTGACAGCATCTCAGCGGCTGAGGAACGCCGGTTTCTTGAGCTCGCCGTTCTGTGAAGAGACAGTCGGGGAAGTGAAGACCTTGCTCGATGAACATGCTAGTGGCTGGGGCATGAAACGTGAAGAAGATATGTTGGTCCTGACGTGGAAGGGGCACAACTCGGTTTTTGCCACAGCTTGGGTCCCAAATCAAAACCGGTTAATGTTGGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

448

Amino Acids

49.48

Weight (kDa)

5.19

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 49 - 436 4.6e-89 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015674)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g43780
malus_domestica MD09G1103000.v1.1 MD17G1090800.v1.1
prunus_persica Prupe.3G222800_v2.0.a1
pyrus_communis pycom09g02620 pycom17g08760
rosa_chinensis RchiOBHm_Chr2g0160531
rosa_laevigata RLG00000021220
rosa_multiflora Rmu_sc0000674.1_g000006
rosa_roxburghii Rroxscaffold_2G00089740
rosa_rugosa Rorug02G0483600
rosa_samantha Rh2AG548900 Rh2BG562600 Rh2CG533200 Rh2DG571900
rosa_wichuraiana Rw2G045440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
AccB1I GGYRCC 2 cut(s) 101, 114
AccB7I CCANNNNNTGG 2 cut(s) 1042, 1305
AccII CGCG 1 cut(s) 377
AciI CCGC 5 cut(s) 336, 339, 377, 380, 1144
AclWI GGATC 2 cut(s) 281, 291
AcsI RAATTY 3 cut(s) 306, 501, 679
AcuI CTGAAG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 4 cut(s) 31, 355, 361, 419
AfiI CCNNNNNNNGG 5 cut(s) 111, 133, 618, 1042, 1305
AflII CTTAAG 1 cut(s) 277
AflIII ACRYGT 1 cut(s) 971
AgeI ACCGGT 2 cut(s) 98, 1323
AgsI TTSAA 3 cut(s) 488, 656, 715
AhlI ACTAGT 1 cut(s) 356
AjiI CACGTC 1 cut(s) 1272
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 6 cut(s) 151, 200, 409, 413, 1170, 1304
AluI AGCT 6 cut(s) 151, 200, 409, 413, 1170, 1304
Alw21I GWGCWC 4 cut(s) 222, 497, 865, 1172
Alw26I GTCTC 5 cut(s) 65, 323, 899, 1004, 1181
AlwI GGATC 2 cut(s) 281, 291
AlwNI CAGNNNCTG 1 cut(s) 1147
Ama87I CYCGRG 2 cut(s) 313, 946
AoxI GGCC 4 cut(s) 542, 580, 620, 795
ApoI RAATTY 3 cut(s) 306, 501, 679
AsiGI ACCGGT 2 cut(s) 98, 1323
AspS9I GGNCC 5 cut(s) 341, 581, 1099, 1264, 1309
AsuHPI GGTGA 4 cut(s) 256, 257, 412, 958
AsuII TTCGAA 1 cut(s) 1092
AsuNHI GCTAGC 1 cut(s) 409
AvaI CYCGRG 2 cut(s) 313, 946
AvaII GGWCC 4 cut(s) 341, 1099, 1264, 1309
BaeGI GKGCMC 4 cut(s) 106, 175, 286, 1284
BanI GGYRCC 2 cut(s) 101, 114
BanII GRGCYC 2 cut(s) 92, 1172
BauI CACGAG 1 cut(s) 166
BbsI GAAGAC 2 cut(s) 659, 1211
Bbv12I GWGCWC 4 cut(s) 222, 497, 865, 1172
BbvCI CCTCAGC 1 cut(s) 1148
BccI CCATC 3 cut(s) 125, 380, 1036
BceAI ACGGC 1 cut(s) 1159
BcgI CGANNNNNNTGC 2 cut(s) 1205, 1239
BciT130I CCWGG 1 cut(s) 271
BciVI GTATCC 1 cut(s) 984
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 5 cut(s) 65, 323, 899, 1004, 1181
BcuI ACTAGT 1 cut(s) 356
BfrI CTTAAG 1 cut(s) 277
BfuI GTATCC 1 cut(s) 984
BisI GCNGC 3 cut(s) 337, 378, 1145
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 3 cut(s) 338, 379, 1146
BmcAI AGTACT 2 cut(s) 355, 361
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 4 cut(s) 341, 1099, 1264, 1309
BmeT110I CYCGRG 2 cut(s) 313, 946
BmgBI CACGTC 1 cut(s) 1272
BmgT120I GGNCC 5 cut(s) 341, 581, 1099, 1264, 1309
BmiI GGNNCC 6 cut(s) 91, 103, 116, 1100, 1310, 1311
BmrFI CCNGG 1 cut(s) 271
BmsI GCATC 4 cut(s) 467, 531, 547, 1145
BmtI GCTAGC 1 cut(s) 413
BoxI GACNNNNGTC 1 cut(s) 1062
BpiI GAAGAC 2 cut(s) 659, 1211
BplI GAGNNNNNCTC 4 cut(s) 102, 134, 716, 748
Bpu10I CCTNAGC 1 cut(s) 1148
Bpu1102I GCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 1092
BpuEI CTTGAG 3 cut(s) 277, 767, 1186
BsaBI GATNNNNATC 1 cut(s) 933
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 118, 434, 798
BsaWI WCCGGW 2 cut(s) 98, 1323
BsaXI ACNNNNNCTCC 2 cut(s) 1037, 1067
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 133, 618, 1042, 1305
Bse118I RCCGGY 3 cut(s) 98, 1157, 1323
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 3 cut(s) 118, 434, 798
BseGI GGATG 4 cut(s) 458, 703, 793, 1047
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 5 cut(s) 111, 133, 618, 1042, 1305
BseMII CTCAG 6 cut(s) 98, 121, 215, 967, 1139, 1154
BseRI GAGGAG 2 cut(s) 400, 721
BseSI GKGCMC 4 cut(s) 106, 175, 286, 1284
BseYI CCCAGC 2 cut(s) 413, 1233
BsgI GTGCAG 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 377
BshFI GGCC 4 cut(s) 544, 582, 622, 797
BshNI GGYRCC 2 cut(s) 101, 114
BshTI ACCGGT 2 cut(s) 98, 1323
BsiHKAI GWGCWC 4 cut(s) 222, 497, 865, 1172
BsiHKCI CYCGRG 2 cut(s) 313, 946
BsiSI CCGG 4 cut(s) 99, 1125, 1158, 1324
BslFI GGGAC 3 cut(s) 614, 686, 1295
BslI CCNNNNNNNGG 5 cut(s) 111, 133, 618, 1042, 1305
BsmAI GTCTC 5 cut(s) 65, 323, 899, 1004, 1181
BsmFI GGGAC 3 cut(s) 614, 686, 1295
BsnI GGCC 4 cut(s) 544, 582, 622, 797
BsoBI CYCGRG 2 cut(s) 313, 946
Bsp119I TTCGAA 1 cut(s) 1092
Bsp1286I GDGCHC 9 cut(s) 92, 106, 175, 222, 286, 497, 865, 1172, 1284
Bsp143I GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 434
BspACI CCGC 5 cut(s) 336, 339, 377, 380, 1144
BspANI GGCC 4 cut(s) 544, 582, 622, 797
BspCNI CTCAG 6 cut(s) 97, 120, 214, 966, 1140, 1153
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 6 cut(s) 91, 103, 116, 1100, 1310, 1311
BspOI GCTAGC 1 cut(s) 413
BspPI GGATC 2 cut(s) 281, 291
BspT104I TTCGAA 1 cut(s) 1092
BspT107I GGYRCC 2 cut(s) 101, 114
BspTI CTTAAG 1 cut(s) 277
BsrFI RCCGGY 3 cut(s) 98, 1157, 1323
BssAI RCCGGY 3 cut(s) 98, 1157, 1323
BssECI CCNNGG 3 cut(s) 118, 434, 798
BssMI GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
BssNI GRCGYC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 166
BssT1I CCWWGG 2 cut(s) 434, 798
Bst2BI CACGAG 1 cut(s) 166
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 5 cut(s) 400, 467, 533, 607, 1192
Bst6I CTCTTC 2 cut(s) 739, 1179
BstACI GRCGYC 1 cut(s) 189
BstAFI CTTAAG 1 cut(s) 277
BstAPI GCANNNNNTGC 1 cut(s) 535
BstBI TTCGAA 1 cut(s) 1092
BstC8I GCNNGC 3 cut(s) 139, 411, 1172
BstDEI CTNAG 8 cut(s) 84, 107, 201, 596, 938, 953, 1140, 1148
BstDSI CCRYGG 1 cut(s) 434
BstEII GGTNACC 1 cut(s) 245
BstENI CCTNNNNNAGG 1 cut(s) 616
BstF5I GGATG 4 cut(s) 458, 703, 793, 1047
BstFNI CGCG 1 cut(s) 377
BstKTI GATC 7 cut(s) 69, 276, 291, 299, 427, 937, 1074
BstMAI GTCTC 5 cut(s) 65, 323, 899, 1004, 1181
BstMBI GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
BstMWI GCNNNNNNNGC 2 cut(s) 123, 535
BstNI CCWGG 1 cut(s) 271
BstNSI RCATGY 2 cut(s) 975, 1226
BstPAI GACNNNNGTC 1 cut(s) 1062
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 269
BstSLI GKGCMC 4 cut(s) 106, 175, 286, 1284
BstUI CGCG 1 cut(s) 377
BstV2I GAAGAC 2 cut(s) 659, 1211
BstX2I RGATCY 2 cut(s) 273, 296
BstYI RGATCY 2 cut(s) 273, 296
BsuI GTATCC 1 cut(s) 984
BsuRI GGCC 4 cut(s) 544, 582, 622, 797
BtgI CCRYGG 1 cut(s) 434
BtgZI GCGATG 1 cut(s) 358
BtrI CACGTC 1 cut(s) 1272
BtsCI GGATG 4 cut(s) 458, 703, 793, 1047
BtsI GCAGTG 2 cut(s) 160, 545
BtsIMutI CAGTG 5 cut(s) 160, 284, 472, 529, 545
Cac8I GCNNGC 3 cut(s) 139, 411, 1172
CaiI CAGNNNCTG 1 cut(s) 1147
Cfr10I RCCGGY 3 cut(s) 98, 1157, 1323
Cfr13I GGNCC 5 cut(s) 341, 581, 1099, 1264, 1309
Csp6I GTAC 4 cut(s) 30, 354, 360, 418
CspAI ACCGGT 2 cut(s) 98, 1323
CspCI CAANNNNNGTGG 2 cut(s) 194, 229
CviAII CATG 5 cut(s) 435, 883, 972, 1223, 1240
CviQI GTAC 4 cut(s) 30, 354, 360, 418
DdeI CTNAG 8 cut(s) 84, 107, 201, 596, 938, 953, 1140, 1148
DpnI GATC 7 cut(s) 68, 275, 290, 298, 426, 936, 1073
DpnII GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
Eam1104I CTCTTC 2 cut(s) 739, 1179
EarI CTCTTC 2 cut(s) 739, 1179
EciI GGCGGA 2 cut(s) 354, 395
Ecl136II GAGCTC 1 cut(s) 1170
Eco130I CCWWGG 2 cut(s) 434, 798
Eco147I AGGCCT 2 cut(s) 622, 797
Eco24I GRGCYC 2 cut(s) 92, 1172
Eco47I GGWCC 4 cut(s) 341, 1099, 1264, 1309
Eco53kI GAGCTC 1 cut(s) 1170
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 2 cut(s) 313, 946
Eco91I GGTNACC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 1170
EcoNI CCTNNNNNAGG 1 cut(s) 616
EcoO109I RGGNCCY 2 cut(s) 581, 1309
EcoO65I GGTNACC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 269
EcoT14I CCWWGG 2 cut(s) 434, 798
EcoT38I GRGCYC 2 cut(s) 92, 1172
ErhI CCWWGG 2 cut(s) 434, 798
FaeI CATG 5 cut(s) 438, 886, 975, 1226, 1243
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 3 cut(s) 614, 686, 1295
FatI CATG 5 cut(s) 434, 882, 971, 1222, 1239
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 337, 378, 1145
FokI GGATG 4 cut(s) 445, 710, 800, 1054
FriOI GRGCYC 2 cut(s) 92, 1172
Fsp4HI GCNGC 3 cut(s) 337, 378, 1145
GluI GCNGC 3 cut(s) 337, 378, 1145
GsaI CCCAGC 2 cut(s) 417, 1237
HaeIII GGCC 4 cut(s) 544, 582, 622, 797
HapII CCGG 4 cut(s) 99, 1125, 1158, 1324
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 5 cut(s) 438, 886, 975, 1226, 1243
HinfI GANTC 9 cut(s) 52, 58, 316, 590, 906, 929, 963, 1052, 1121
HpaII CCGG 4 cut(s) 99, 1125, 1158, 1324
HphI GGTGA 4 cut(s) 256, 257, 412, 958
Hpy166II GTNNAC 1 cut(s) 922
Hpy188I TCNGA 7 cut(s) 57, 110, 122, 838, 934, 1057, 1068
Hpy8I GTNNAC 1 cut(s) 922
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 572, 1088, 1270
HpyCH4III ACNGT 5 cut(s) 400, 467, 533, 607, 1192
HpyCH4IV ACGT 5 cut(s) 32, 189, 420, 1246, 1271
HpyCH4V TGCA 5 cut(s) 141, 165, 458, 470, 538
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 535
HpyF3I CTNAG 8 cut(s) 84, 107, 201, 596, 938, 953, 1140, 1148
HpySE526I ACGT 5 cut(s) 32, 189, 420, 1246, 1271
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 5 cut(s) 438, 886, 975, 1226, 1243
KflI GGGWCCC 1 cut(s) 1309
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
LmnI GCTCC 2 cut(s) 95, 406
LweI GCATC 4 cut(s) 467, 531, 547, 1145
MaeII ACGT 5 cut(s) 32, 189, 420, 1246, 1271
MaeIII GTNAC 8 cut(s) 190, 245, 400, 601, 822, 866, 1031, 1129
MalI GATC 7 cut(s) 68, 275, 290, 298, 426, 936, 1073
MboI GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 2 cut(s) 273, 296
MhlI GDGCHC 9 cut(s) 92, 106, 175, 222, 286, 497, 865, 1172, 1284
MlyI GAGTC 4 cut(s) 67, 325, 1061, 1130
MmeI TCCRAC 3 cut(s) 486, 1080, 1314
MseI TTAA 7 cut(s) 278, 761, 776, 879, 978, 1328, 1342
MslI CAYNNNNRTG 1 cut(s) 1227
MspA1I CMGCKG 1 cut(s) 1144
MspCI CTTAAG 1 cut(s) 277
MspI CCGG 4 cut(s) 99, 1125, 1158, 1324
MspR9I CCNGG 1 cut(s) 271
MunI CAATTG 1 cut(s) 651
MvaI CCWGG 1 cut(s) 271
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 2 cut(s) 123, 535
NcoI CCATGG 1 cut(s) 434
NdeII GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
NheI GCTAGC 1 cut(s) 409
NlaIII CATG 5 cut(s) 438, 886, 975, 1226, 1243
NlaIV GGNNCC 6 cut(s) 91, 103, 116, 1100, 1310, 1311
NmuCI GTSAC 5 cut(s) 190, 245, 400, 601, 1129
NspI RCATGY 2 cut(s) 975, 1226
NspV TTCGAA 1 cut(s) 1092
PceI AGGCCT 2 cut(s) 622, 797
PciI ACATGT 1 cut(s) 971
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 921, 930
PfeI GAWTC 5 cut(s) 52, 590, 906, 929, 963
PflMI CCANNNNNTGG 2 cut(s) 1042, 1305
PfoI TCCNGGA 1 cut(s) 269
PinAI ACCGGT 2 cut(s) 98, 1323
PkrI GCNGC 3 cut(s) 338, 379, 1146
PleI GAGTC 4 cut(s) 66, 324, 1060, 1129
PpsI GAGTC 4 cut(s) 66, 324, 1060, 1129
PpuMI RGGWCCY 1 cut(s) 1309
PscI ACATGT 1 cut(s) 971
PshAI GACNNNNGTC 1 cut(s) 1062
Psp124BI GAGCTC 1 cut(s) 1172
Psp5II RGGWCCY 1 cut(s) 1309
Psp6I CCWGG 1 cut(s) 269
PspEI GGTNACC 1 cut(s) 245
PspFI CCCAGC 2 cut(s) 413, 1233
PspGI CCWGG 1 cut(s) 269
PspN4I GGNNCC 6 cut(s) 91, 103, 116, 1100, 1310, 1311
PspPI GGNCC 5 cut(s) 341, 581, 1099, 1264, 1309
PspPPI RGGWCCY 1 cut(s) 1309
PstNI CAGNNNCTG 1 cut(s) 1147
PsuI RGATCY 2 cut(s) 273, 296
RsaI GTAC 4 cut(s) 31, 355, 361, 419
RsaNI GTAC 4 cut(s) 30, 354, 360, 418
RseI CAYNNNNRTG 1 cut(s) 1227
SacI GAGCTC 1 cut(s) 1172
SaqAI TTAA 7 cut(s) 278, 761, 776, 879, 978, 1328, 1342
SatI GCNGC 3 cut(s) 337, 378, 1145
Sau3AI GATC 7 cut(s) 66, 273, 288, 296, 424, 934, 1071
Sau96I GGNCC 5 cut(s) 341, 581, 1099, 1264, 1309
ScaI AGTACT 2 cut(s) 355, 361
SchI GAGTC 4 cut(s) 67, 325, 1061, 1130
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 9 cut(s) 92, 106, 175, 222, 286, 497, 865, 1172, 1284
SfaNI GCATC 4 cut(s) 467, 531, 547, 1145
SfuI TTCGAA 1 cut(s) 1092
SinI GGWCC 4 cut(s) 341, 1099, 1264, 1309
SmiMI CAYNNNNRTG 1 cut(s) 1227
SmlI CTYRAG 4 cut(s) 277, 292, 782, 1165
SmoI CTYRAG 4 cut(s) 277, 292, 782, 1165
SpeI ACTAGT 1 cut(s) 356
SseBI AGGCCT 2 cut(s) 622, 797
SsiI CCGC 5 cut(s) 336, 339, 377, 380, 1144
SstI GAGCTC 1 cut(s) 1172
StuI AGGCCT 2 cut(s) 622, 797
StyD4I CCNGG 1 cut(s) 269
StyI CCWWGG 2 cut(s) 434, 798
TaaI ACNGT 5 cut(s) 400, 467, 533, 607, 1192
TaiI ACGT 5 cut(s) 35, 192, 423, 1249, 1274
TaqI TCGA 7 cut(s) 129, 723, 741, 959, 1074, 1092, 1215
TaqII GACCGA 1 cut(s) 653
TatI WGTACW 2 cut(s) 353, 359
TauI GCSGC 3 cut(s) 339, 380, 1147
TfiI GAWTC 5 cut(s) 52, 590, 906, 929, 963
Tru1I TTAA 7 cut(s) 278, 761, 776, 879, 978, 1328, 1342
Tru9I TTAA 7 cut(s) 278, 761, 776, 879, 978, 1328, 1342
TscAI CASTG 5 cut(s) 167, 291, 472, 536, 545
TseFI GTSAC 5 cut(s) 190, 245, 400, 601, 1129
Tsp45I GTSAC 5 cut(s) 190, 245, 400, 601, 1129
TspDTI ATGAA 4 cut(s) 17, 494, 1233, 1256
TspGWI ACGGA 2 cut(s) 345, 419
TspRI CASTG 5 cut(s) 167, 291, 472, 536, 545
Van91I CCANNNNNTGG 2 cut(s) 1042, 1305
Vha464I CTTAAG 1 cut(s) 277
VpaK11BI GGWCC 4 cut(s) 341, 1099, 1264, 1309
XagI CCTNNNNNAGG 1 cut(s) 616
XapI RAATTY 3 cut(s) 306, 501, 679
XbaI TCTAGA 1 cut(s) 302
XceI RCATGY 2 cut(s) 975, 1226
ZraI GACGTC 1 cut(s) 190
ZrmI AGTACT 2 cut(s) 355, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.