Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0015804

Physical Location & Seq

Genomic Coordinates Reverse (-)

8566858 .. 8571273

Gene Structure 4416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1112800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGTACTCCATGAATGGCAAGGCAGTGCCTGGTCGCTTCGTCTCTTCATACAGCGAGGTCCAGGCCAGTCGTCTCGACGTTCCGCTTCCTCTCCCTTCTGTTCTGAAGAGCTCCTTCAGCGTCGTCGATGGCCCCAAAAGCTCCGCCGCCGGAAACCCAGAGGAAATAGCCAAGTTGTTTCCTAACCTATTTGGGCAGCCTTCAGCAATGCTACGGCCAGGCGGTTCTTCGGTGCTTCCGAAGGATAAGAGTTTGAAGATTGGAGTGGTTCTCTCAGGAGGACAGGCCCCCGGTGGACACAATGTTATTTGTGGGATATTTGATTACTTGCAGAAGCACACTAGTGGAAGCATAATGTATGGATTTAAGGGTGGTCCGGCAGGGATCATGAAGTGTAAATACGTTCAACTGTCCGCGGAATTCATCTACCCTTACAGAAATCAGGGTGGCTTTGACATGATTGCAAGCGGCAGAGACAAGATTGAAACTCCCGAACAGTTTAAGCTAGCAGAAGAAACAGCAAAGAAGTTTGATTTAGACGGGCTTGTGGTCATTGGCGGGGATGACTCAAATACCAATGCTTGCCTTCTAGCTGAACACTTCAGGGGTAAAAATATGAAGACACAGGTAATTGGATGTCCCAAAACCATTGATGGTGATCTGAAAAGCAAAGAGGTGCCCATAAGTTTTGGATTCGACACTGCATGCAAGCTATATTCAGAAATGATTGGTAACGTGATGATGGATGCCCGTTCAACTGGGAAGTACTACCACTTTGTAAGGCTTATGGGGCGTGCAGCTTCTCATATAACCTTGGAGTGTGCACTGCAGACTCGTCCAAACGTCACGATTATTGGGGAAGAGGTTGCTGCCAAGAAGCAGACACTCAAGAGTGTAACAGACTATATAACAGATATAATCTGCAAACGTTCGGAACTTGGGTTTAATTATGGTGTCATACTTATACCAGAAGGCCTGATTGATTTCATTCCAGAGGTGCAGCAACTAATTTCGGAGCTTAACGAAATCTTAGCTCATGATATTGTTGATGAAGCAGGGGTGTGGAAACAGAAACTCCAGAGCCAATCCCTTGAGCTTTTTGAGTTTTTGCCGCAAACAATTCAGGAGCAACTACTGCTCGAAAGAGATCCACACGGGAATGTGCAGGTAGCAAAAATAGAGACAGAAAAGATGCTCCTTCAAATGGTAGAAACTGAACTGCACAAACGGATGCAGGAGGGGACATATAAGCGTGACTTTGCTGGAAAGACACACTTCTTCGGTTATGAGGGCAGATGTGGTCTTCCTACAAACTTTGATGCAAACTACTGCTATGCATTAGGTTATGGTGCCGGTGCGCTCCTTCATTCAGGAAAGACAGGATTGATATCCTCTGTGGGAAATCTGGCTGCGCCGGTTGAGCAATGGACAGTTGGTGGCACGGCATTGACTTCTTTAATGGATGTGGAGCGGAGACATGGAAAGTTCAAGCCTGTGATTAAGAAAGCAATGGTGGAACTTGAAGGTGCACCATTCAAAAAATTTGCATCATTCCGGGACGAGTGGGCTCTTAAGAATCGCTACGTTAATCCGGGTCCGATTCAATTTGTTGGGTCCGCAGCAAATACGATCAACAACACTCTTCTGCTGGAACTTGGAGCACAAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008443 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0015979 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0042221 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046835 GO:0047334 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko01100 ko01110 ko01120 ko01130 map00010 map00030 map00051 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

PFK

Protein Analysis

557

Amino Acids

60.88

Weight (kDa)

7.15

Isoelectric Point (pI)

35.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	86 - 451	8.5e-35	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04040
fragaria_vesca	FvH4_6g42470
malus_domestica	MD09G1112800.v1.1
prunus_persica	Prupe.3G211800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0158381 RchiOBHm_Chr4g0419181
rosa_laevigata	RLG00000021081
rosa_multiflora	Rmu_sc0001826.1_g000016 Rmu_sc0005921.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091450
rosa_rugosa	Rorug02G0467400
rosa_samantha	Rh2DG556900 Rh4DG218400
rosa_wichuraiana	Rw2G044220 Rw4G018730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1156
AccB1I	GGYRCC	2 cut(s)	676, 1349
AccBSI	CCGCTC	1 cut(s)	1471
AccII	CGCG	1 cut(s)	416
AclI	AACGTT	1 cut(s)	928
AclWI	GGATC	2 cut(s)	392, 1142
AcoI	YGGCCR	1 cut(s)	215
AcsI	RAATTY	2 cut(s)	419, 1541
AcuI	CTGAAG	4 cut(s)	100, 125, 186, 586
AfaI	GTAC	2 cut(s)	5, 767
AfiI	CCNNNNNNNGG	1 cut(s)	1204
AflII	CTTAAG	1 cut(s)	1571
AgsI	TTSAA	9 cut(s)	256, 407, 485, 756, 1202, 1489, 1523, 1537, 1604
AhlI	ACTAGT	1 cut(s)	341
AjnI	CCWGG	3 cut(s)	28, 60, 217
AleI	CACNNNNGTG	1 cut(s)	342
AluBI	AGCT	9 cut(s)	111, 141, 505, 593, 712, 800, 1018, 1034, 1096
AluI	AGCT	9 cut(s)	111, 141, 505, 593, 712, 800, 1018, 1034, 1096
Alw21I	GWGCWC	4 cut(s)	113, 826, 1531, 1663
Alw26I	GTCTC	5 cut(s)	46, 77, 468, 1175, 1468
Alw44I	GTGCAC	2 cut(s)	822, 1527
AlwI	GGATC	2 cut(s)	392, 1142
AlwNI	CAGNNNCTG	1 cut(s)	29
AoxI	GGCC	5 cut(s)	63, 130, 215, 285, 973
ApaLI	GTGCAC	2 cut(s)	822, 1527
ApeKI	GCWGC	6 cut(s)	196, 797, 869, 1000, 1409, 1619
ApoI	RAATTY	2 cut(s)	419, 1541
AspLEI	GCGC	2 cut(s)	1360, 1414
AspS9I	GGNCC	6 cut(s)	58, 131, 286, 374, 1595, 1614
AsuC2I	CCSGG	3 cut(s)	291, 1556, 1593
AsuHPI	GGTGA	1 cut(s)	668
AsuNHI	GCTAGC	1 cut(s)	505
AvaII	GGWCC	4 cut(s)	58, 374, 1595, 1614
BaeGI	GKGCMC	3 cut(s)	681, 826, 1531
BanI	GGYRCC	2 cut(s)	676, 1349
BanII	GRGCYC	2 cut(s)	113, 1570
BarI	GAAGNNNNNNTAC	2 cut(s)	383, 415
BbsI	GAAGAC	2 cut(s)	626, 1295
Bbv12I	GWGCWC	4 cut(s)	113, 826, 1531, 1663
BbvI	GCAGC	6 cut(s)	208, 809, 856, 1012, 1396, 1631
BccI	CCATC	3 cut(s)	122, 647, 736
BceAI	ACGGC	2 cut(s)	230, 1458
BciT130I	CCWGG	3 cut(s)	30, 62, 219
BcnI	CCSGG	3 cut(s)	291, 1556, 1593
BcoDI	GTCTC	5 cut(s)	46, 77, 468, 1175, 1468
BcuI	ACTAGT	1 cut(s)	341
BfaI	CTAG	3 cut(s)	342, 506, 590
BfmI	CTRYAG	1 cut(s)	827
BfrI	CTTAAG	1 cut(s)	1571
BfuAI	ACCTGC	1 cut(s)	1156
BisI	GCNGC	9 cut(s)	147, 197, 469, 798, 870, 1001, 1112, 1410, 1620
BlsI	GCNGC	9 cut(s)	148, 198, 470, 799, 871, 1002, 1113, 1411, 1621
BmcAI	AGTACT	1 cut(s)	767
Bme1390I	CCNGG	6 cut(s)	30, 62, 219, 291, 1556, 1593
Bme18I	GGWCC	4 cut(s)	58, 374, 1595, 1614
BmgT120I	GGNCC	6 cut(s)	58, 131, 286, 374, 1595, 1614
BmiI	GGNNCC	6 cut(s)	133, 288, 678, 1351, 1596, 1615
BmrFI	CCNGG	6 cut(s)	30, 62, 219, 291, 1556, 1593
BmrI	ACTGGG	1 cut(s)	768
BmsI	GCATC	5 cut(s)	736, 1182, 1221, 1309, 1556
BmtI	GCTAGC	1 cut(s)	509
BmuI	ACTGGG	1 cut(s)	768
BpiI	GAAGAC	2 cut(s)	626, 1295
BplI	GAGNNNNNCTC	2 cut(s)	255, 287
BpmI	CTGGAG	1 cut(s)	1061
BpuEI	CTTGAG	2 cut(s)	872, 1112
BpuMI	CCSGG	3 cut(s)	291, 1556, 1593
BsaBI	GATNNNNATC	2 cut(s)	657, 1387
BsaJI	CCNNGG	3 cut(s)	289, 414, 813
BsaXI	ACNNNNNCTCC	2 cut(s)	1059, 1089
Bsc4I	CCNNNNNNNGG	1 cut(s)	1204
Bse118I	RCCGGY	2 cut(s)	1352, 1414
Bse1I	ACTGG	2 cut(s)	66, 763
Bse3DI	GCAATG	3 cut(s)	213, 1430, 1515
Bse8I	GATNNNNATC	2 cut(s)	657, 1387
BseBI	CCWGG	3 cut(s)	30, 62, 219
BseDI	CCNNGG	3 cut(s)	289, 414, 813
BseGI	GGATG	5 cut(s)	568, 641, 751, 1236, 1468
BseJI	GATNNNNATC	2 cut(s)	657, 1387
BseLI	CCNNNNNNNGG	1 cut(s)	1204
BseMI	GCAATG	3 cut(s)	213, 1430, 1515
BseMII	CTCAG	1 cut(s)	288
BseNI	ACTGG	2 cut(s)	66, 763
BseSI	GKGCMC	3 cut(s)	681, 826, 1531
BseXI	GCAGC	6 cut(s)	208, 809, 856, 1012, 1396, 1631
BsgI	GTGCAG	4 cut(s)	816, 1019, 1184, 1205
Bsh1236I	CGCG	1 cut(s)	416
BshFI	GGCC	5 cut(s)	65, 132, 217, 287, 975
BshNI	GGYRCC	2 cut(s)	676, 1349
BsiHKAI	GWGCWC	4 cut(s)	113, 826, 1531, 1663
BsiSI	CCGG	7 cut(s)	150, 291, 377, 1353, 1415, 1555, 1592
BslFI	GGGAC	3 cut(s)	624, 1255, 1571
BslI	CCNNNNNNNGG	1 cut(s)	1204
BsmAI	GTCTC	5 cut(s)	46, 77, 468, 1175, 1468
BsmBI	CGTCTC	2 cut(s)	46, 77
BsmFI	GGGAC	3 cut(s)	624, 1255, 1571
BsnI	GGCC	5 cut(s)	65, 132, 217, 287, 975
Bsp1286I	GDGCHC	6 cut(s)	113, 681, 826, 1531, 1570, 1663
Bsp143I	GATC	4 cut(s)	384, 658, 1147, 1629
BspANI	GGCC	5 cut(s)	65, 132, 217, 287, 975
BspCNI	CTCAG	1 cut(s)	287
BspFNI	CGCG	1 cut(s)	416
BspHI	TCATGA	2 cut(s)	387, 1036
BspLI	GGNNCC	6 cut(s)	133, 288, 678, 1351, 1596, 1615
BspMAI	CTGCAG	1 cut(s)	831
BspMI	ACCTGC	1 cut(s)	1156
BspOI	GCTAGC	1 cut(s)	509
BspPI	GGATC	2 cut(s)	392, 1142
BspQI	GCTCTTC	1 cut(s)	101
BspT107I	GGYRCC	2 cut(s)	676, 1349
BspTI	CTTAAG	1 cut(s)	1571
BsrBI	CCGCTC	1 cut(s)	1471
BsrDI	GCAATG	3 cut(s)	213, 1430, 1515
BsrFI	RCCGGY	2 cut(s)	1352, 1414
BsrI	ACTGG	2 cut(s)	66, 763
BssAI	RCCGGY	2 cut(s)	1352, 1414
BssECI	CCNNGG	3 cut(s)	289, 414, 813
BssMI	GATC	4 cut(s)	384, 658, 1147, 1629
BssT1I	CCWWGG	1 cut(s)	813
Bst2UI	CCWGG	3 cut(s)	30, 62, 219
Bst4CI	ACNGT	3 cut(s)	411, 498, 1432
Bst6I	CTCTTC	4 cut(s)	49, 101, 855, 1647
BstAFI	CTTAAG	1 cut(s)	1571
BstAPI	GCANNNNNTGC	1 cut(s)	1135
BstC8I	GCNNGC	6 cut(s)	466, 507, 583, 706, 710, 795
BstDEI	CTNAG	2 cut(s)	274, 1030
BstDSI	CCRYGG	1 cut(s)	414
BstF5I	GGATG	5 cut(s)	568, 641, 751, 1236, 1468
BstFNI	CGCG	1 cut(s)	416
BstHHI	GCGC	2 cut(s)	1360, 1414
BstKTI	GATC	4 cut(s)	387, 661, 1150, 1632
BstMAI	GTCTC	5 cut(s)	46, 77, 468, 1175, 1468
BstMBI	GATC	4 cut(s)	384, 658, 1147, 1629
BstMWI	GCNNNNNNNGC	5 cut(s)	117, 138, 790, 1135, 1420
BstNI	CCWGG	3 cut(s)	30, 62, 219
BstNSI	RCATGY	1 cut(s)	708
BstSCI	CCNGG	6 cut(s)	28, 60, 217, 289, 1554, 1591
BstSFI	CTRYAG	1 cut(s)	827
BstSLI	GKGCMC	3 cut(s)	681, 826, 1531
BstUI	CGCG	1 cut(s)	416
BstV1I	GCAGC	6 cut(s)	208, 809, 856, 1012, 1396, 1631
BstV2I	GAAGAC	2 cut(s)	626, 1295
BstX2I	RGATCY	1 cut(s)	1147
BstYI	RGATCY	1 cut(s)	1147
BsuRI	GGCC	5 cut(s)	65, 132, 217, 287, 975
BtgI	CCRYGG	1 cut(s)	414
BtsCI	GGATG	5 cut(s)	568, 641, 751, 1236, 1468
BtsI	GCAGTG	3 cut(s)	30, 699, 824
BtsIMutI	CAGTG	3 cut(s)	30, 699, 824
BveI	ACCTGC	1 cut(s)	1156
Cac8I	GCNNGC	6 cut(s)	466, 507, 583, 706, 710, 795
CaiI	CAGNNNCTG	1 cut(s)	29
CciI	TCATGA	2 cut(s)	387, 1036
CfoI	GCGC	2 cut(s)	1360, 1414
Cfr10I	RCCGGY	2 cut(s)	1352, 1414
Cfr13I	GGNCC	6 cut(s)	58, 131, 286, 374, 1595, 1614
Cfr42I	CCGCGG	1 cut(s)	417
CseI	GACGC	1 cut(s)	109
Csp6I	GTAC	2 cut(s)	4, 766
CviAII	CATG	6 cut(s)	10, 388, 457, 705, 1037, 1478
CviQI	GTAC	2 cut(s)	4, 766
DdeI	CTNAG	2 cut(s)	274, 1030
DpnI	GATC	4 cut(s)	386, 660, 1149, 1631
DpnII	GATC	4 cut(s)	384, 658, 1147, 1629
EaeI	YGGCCR	1 cut(s)	215
Eam1104I	CTCTTC	4 cut(s)	49, 101, 855, 1647
EarI	CTCTTC	4 cut(s)	49, 101, 855, 1647
EciI	GGCGGA	1 cut(s)	133
Ecl136II	GAGCTC	1 cut(s)	111
Eco130I	CCWWGG	1 cut(s)	813
Eco147I	AGGCCT	1 cut(s)	975
Eco24I	GRGCYC	2 cut(s)	113, 1570
Eco32I	GATATC	1 cut(s)	1389
Eco47I	GGWCC	4 cut(s)	58, 374, 1595, 1614
Eco53kI	GAGCTC	1 cut(s)	111
Eco57I	CTGAAG	4 cut(s)	100, 125, 186, 586
EcoICRI	GAGCTC	1 cut(s)	111
EcoO109I	RGGNCCY	1 cut(s)	286
EcoRI	GAATTC	1 cut(s)	419
EcoRII	CCWGG	3 cut(s)	28, 60, 217
EcoRV	GATATC	1 cut(s)	1389
EcoT14I	CCWWGG	1 cut(s)	813
EcoT22I	ATGCAT	1 cut(s)	1339
EcoT38I	GRGCYC	2 cut(s)	113, 1570
ErhI	CCWWGG	1 cut(s)	813
Esp3I	CGTCTC	2 cut(s)	46, 77
FaeI	CATG	6 cut(s)	13, 391, 460, 708, 1040, 1481
FalI	AAGNNNNNCTT	6 cut(s)	98, 130, 1241, 1273, 1259, 1291
FaqI	GGGAC	3 cut(s)	624, 1255, 1571
FatI	CATG	6 cut(s)	9, 387, 456, 704, 1036, 1477
FauI	CCCGC	1 cut(s)	551
Fnu4HI	GCNGC	9 cut(s)	147, 197, 469, 798, 870, 1001, 1112, 1410, 1620
FokI	GGATG	5 cut(s)	575, 648, 758, 1243, 1475
FriOI	GRGCYC	2 cut(s)	113, 1570
Fsp4HI	GCNGC	9 cut(s)	147, 197, 469, 798, 870, 1001, 1112, 1410, 1620
FspBI	CTAG	3 cut(s)	342, 506, 590
GlaI	GCGC	2 cut(s)	1359, 1413
GluI	GCNGC	9 cut(s)	147, 197, 469, 798, 870, 1001, 1112, 1410, 1620
GsuI	CTGGAG	1 cut(s)	1061
HaeIII	GGCC	5 cut(s)	65, 132, 217, 287, 975
HapII	CCGG	7 cut(s)	150, 291, 377, 1353, 1415, 1555, 1592
HgaI	GACGC	1 cut(s)	109
HhaI	GCGC	2 cut(s)	1360, 1414
Hin1II	CATG	6 cut(s)	13, 391, 460, 708, 1040, 1481
Hin6I	GCGC	2 cut(s)	1358, 1412
HinP1I	GCGC	2 cut(s)	1358, 1412
HinfI	GANTC	5 cut(s)	566, 693, 832, 1576, 1600
HpaII	CCGG	7 cut(s)	150, 291, 377, 1353, 1415, 1555, 1592
HphI	GGTGA	1 cut(s)	668
Hpy166II	GTNNAC	3 cut(s)	296, 824, 1529
Hpy188I	TCNGA	7 cut(s)	105, 240, 663, 721, 934, 1015, 1599
Hpy8I	GTNNAC	3 cut(s)	296, 824, 1529
Hpy99I	CGWCG	3 cut(s)	80, 125, 128
HpyAV	CCTTC	9 cut(s)	105, 124, 210, 235, 596, 965, 1208, 1373, 1517
HpyCH4III	ACNGT	3 cut(s)	411, 498, 1432
HpyCH4IV	ACGT	6 cut(s)	78, 402, 735, 843, 928, 1584
HpyF10VI	GCNNNNNNNGC	5 cut(s)	117, 138, 790, 1135, 1420
HpyF3I	CTNAG	2 cut(s)	274, 1030
HpySE526I	ACGT	6 cut(s)	78, 402, 735, 843, 928, 1584
Hsp92II	CATG	6 cut(s)	13, 391, 460, 708, 1040, 1481
HspAI	GCGC	2 cut(s)	1358, 1412
KspI	CCGCGG	1 cut(s)	417
Kzo9I	GATC	4 cut(s)	384, 658, 1147, 1629
LguI	GCTCTTC	1 cut(s)	101
LmnI	GCTCC	8 cut(s)	116, 146, 1015, 1126, 1200, 1365, 1468, 1658
Lsp1109I	GCAGC	6 cut(s)	208, 809, 856, 1012, 1396, 1631
LweI	GCATC	5 cut(s)	736, 1182, 1221, 1309, 1556
MaeI	CTAG	3 cut(s)	342, 506, 590
MaeII	ACGT	6 cut(s)	78, 402, 735, 843, 928, 1584
MaeIII	GTNAC	4 cut(s)	731, 844, 895, 1253
MalI	GATC	4 cut(s)	386, 660, 1149, 1631
MbiI	CCGCTC	1 cut(s)	1471
MboI	GATC	4 cut(s)	384, 658, 1147, 1629
MflI	RGATCY	1 cut(s)	1147
MhlI	GDGCHC	6 cut(s)	113, 681, 826, 1531, 1570, 1663
MluCI	AATT	7 cut(s)	419, 630, 946, 1008, 1119, 1541, 1604
MlyI	GAGTC	2 cut(s)	560, 826
Mph1103I	ATGCAT	1 cut(s)	1339
MseI	TTAA	8 cut(s)	366, 501, 945, 1020, 1457, 1500, 1572, 1587
MslI	CAYNNNNRTG	2 cut(s)	342, 1158
MspA1I	CMGCKG	1 cut(s)	416
MspCI	CTTAAG	1 cut(s)	1571
MspI	CCGG	7 cut(s)	150, 291, 377, 1353, 1415, 1555, 1592
MspR9I	CCNGG	6 cut(s)	30, 62, 219, 291, 1556, 1593
MvaI	CCWGG	3 cut(s)	30, 62, 219
MvnI	CGCG	1 cut(s)	416
MwoI	GCNNNNNNNGC	5 cut(s)	117, 138, 790, 1135, 1420
NciI	CCSGG	3 cut(s)	291, 1556, 1593
NdeII	GATC	4 cut(s)	384, 658, 1147, 1629
NheI	GCTAGC	1 cut(s)	505
NlaIII	CATG	6 cut(s)	13, 391, 460, 708, 1040, 1481
NlaIV	GGNNCC	6 cut(s)	133, 288, 678, 1351, 1596, 1615
NmuCI	GTSAC	2 cut(s)	844, 1253
NsiI	ATGCAT	1 cut(s)	1339
NspI	RCATGY	1 cut(s)	708
OliI	CACNNNNGTG	1 cut(s)	342
PaeI	GCATGC	1 cut(s)	708
PagI	TCATGA	2 cut(s)	387, 1036
PceI	AGGCCT	1 cut(s)	975
PciSI	GCTCTTC	1 cut(s)	101
PcsI	WCGNNNNNNNCGW	1 cut(s)	236
PfeI	GAWTC	3 cut(s)	693, 1576, 1600
PfoI	TCCNGGA	1 cut(s)	1554
PkrI	GCNGC	9 cut(s)	148, 198, 470, 799, 871, 1002, 1113, 1411, 1621
PleI	GAGTC	2 cut(s)	560, 826
PpsI	GAGTC	2 cut(s)	560, 826
Psp124BI	GAGCTC	1 cut(s)	113
Psp1406I	AACGTT	1 cut(s)	928
Psp6I	CCWGG	3 cut(s)	28, 60, 217
PspGI	CCWGG	3 cut(s)	28, 60, 217
PspN4I	GGNNCC	6 cut(s)	133, 288, 678, 1351, 1596, 1615
PspPI	GGNCC	6 cut(s)	58, 131, 286, 374, 1595, 1614
PstI	CTGCAG	1 cut(s)	831
PstNI	CAGNNNCTG	1 cut(s)	29
PsuI	RGATCY	1 cut(s)	1147
RsaI	GTAC	2 cut(s)	5, 767
RsaNI	GTAC	2 cut(s)	4, 766
RseI	CAYNNNNRTG	2 cut(s)	342, 1158
SacI	GAGCTC	1 cut(s)	113
SacII	CCGCGG	1 cut(s)	417
SapI	GCTCTTC	1 cut(s)	101
SaqAI	TTAA	8 cut(s)	366, 501, 945, 1020, 1457, 1500, 1572, 1587
SatI	GCNGC	9 cut(s)	147, 197, 469, 798, 870, 1001, 1112, 1410, 1620
Sau3AI	GATC	4 cut(s)	384, 658, 1147, 1629
Sau96I	GGNCC	6 cut(s)	58, 131, 286, 374, 1595, 1614
ScaI	AGTACT	1 cut(s)	767
SchI	GAGTC	2 cut(s)	560, 826
ScrFI	CCNGG	6 cut(s)	30, 62, 219, 291, 1556, 1593
SduI	GDGCHC	6 cut(s)	113, 681, 826, 1531, 1570, 1663
SfaNI	GCATC	5 cut(s)	736, 1182, 1221, 1309, 1556
SfcI	CTRYAG	1 cut(s)	827
Sfr303I	CCGCGG	1 cut(s)	417
SgrBI	CCGCGG	1 cut(s)	417
SinI	GGWCC	4 cut(s)	58, 374, 1595, 1614
SmiMI	CAYNNNNRTG	2 cut(s)	342, 1158
SmlI	CTYRAG	3 cut(s)	887, 1091, 1571
SmoI	CTYRAG	3 cut(s)	887, 1091, 1571
SpeI	ACTAGT	1 cut(s)	341
SphI	GCATGC	1 cut(s)	708
Sse9I	AATT	7 cut(s)	419, 630, 946, 1008, 1119, 1541, 1604
SseBI	AGGCCT	1 cut(s)	975
SspMI	CTAG	3 cut(s)	342, 506, 590
SstI	GAGCTC	1 cut(s)	113
StuI	AGGCCT	1 cut(s)	975
StyD4I	CCNGG	6 cut(s)	28, 60, 217, 289, 1554, 1591
StyI	CCWWGG	1 cut(s)	813
TaaI	ACNGT	3 cut(s)	411, 498, 1432
TaiI	ACGT	6 cut(s)	81, 405, 738, 846, 931, 1587
TaqI	TCGA	4 cut(s)	75, 126, 696, 1140
TasI	AATT	7 cut(s)	419, 630, 946, 1008, 1119, 1541, 1604
TatI	WGTACW	2 cut(s)	3, 765
TauI	GCSGC	3 cut(s)	149, 471, 1114
TfiI	GAWTC	3 cut(s)	693, 1576, 1600
Tru1I	TTAA	8 cut(s)	366, 501, 945, 1020, 1457, 1500, 1572, 1587
Tru9I	TTAA	8 cut(s)	366, 501, 945, 1020, 1457, 1500, 1572, 1587
TscAI	CASTG	3 cut(s)	30, 706, 831
TseFI	GTSAC	2 cut(s)	844, 1253
TseI	GCWGC	6 cut(s)	196, 797, 869, 1000, 1409, 1619
Tsp45I	GTSAC	2 cut(s)	844, 1253
TspDTI	ATGAA	8 cut(s)	26, 36, 404, 412, 632, 976, 1065, 1355
TspGWI	ACGGA	1 cut(s)	1243
TspRI	CASTG	3 cut(s)	30, 706, 831
Vha464I	CTTAAG	1 cut(s)	1571
VneI	GTGCAC	2 cut(s)	822, 1527
VpaK11BI	GGWCC	4 cut(s)	58, 374, 1595, 1614
XapI	RAATTY	2 cut(s)	419, 1541
XceI	RCATGY	1 cut(s)	708
XspI	CTAG	3 cut(s)	342, 506, 590
ZrmI	AGTACT	1 cut(s)	767
Zsp2I	ATGCAT	1 cut(s)	1339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.