Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G04040 |
| fragaria_vesca | FvH4_6g42470 |
| malus_domestica | MD09G1112800.v1.1 |
| prunus_persica | Prupe.3G211800_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr2g0158381 RchiOBHm_Chr4g0419181 |
| rosa_laevigata | RLG00000021081 |
| rosa_multiflora | Rmu_sc0001826.1_g000016 Rmu_sc0005921.1_g000001 |
| rosa_roxburghii | Rroxscaffold_2G00091450 |
| rosa_rugosa | Rorug02G0467400 |
| rosa_samantha | Rh2DG556900 Rh4DG218400 |
| rosa_wichuraiana | Rw2G044220 Rw4G018730 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 325 |
| AccBSI | CCGCTC | 1 cut(s) | 159 |
| AccI | GTMKAC | 2 cut(s) | 171, 249 |
| AciI | CCGC | 8 cut(s) | 23, 41, 133, 157, 162, 253, 267, 270 |
| AcoI | YGGCCR | 2 cut(s) | 154, 316 |
| AcuI | CTGAAG | 1 cut(s) | 401 |
| AcyI | GRCGYC | 1 cut(s) | 329 |
| AdeI | CACNNNGTG | 1 cut(s) | 415 |
| AfaI | GTAC | 1 cut(s) | 572 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 325 |
| AgsI | TTSAA | 1 cut(s) | 474 |
| AjnI | CCWGG | 1 cut(s) | 151 |
| AluBI | AGCT | 1 cut(s) | 264 |
| AluI | AGCT | 1 cut(s) | 264 |
| Alw26I | GTCTC | 1 cut(s) | 200 |
| AoxI | GGCC | 4 cut(s) | 145, 154, 316, 439 |
| ApeKI | GCWGC | 2 cut(s) | 563, 566 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 178, 210 |
| AspS9I | GGNCC | 1 cut(s) | 439 |
| AsuC2I | CCSGG | 1 cut(s) | 443 |
| AsuHPI | GGTGA | 3 cut(s) | 356, 446, 457 |
| AsuII | TTCGAA | 1 cut(s) | 28 |
| BbvI | GCAGC | 1 cut(s) | 575 |
| BceAI | ACGGC | 3 cut(s) | 127, 132, 177 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 474, 508 |
| BciT130I | CCWGG | 1 cut(s) | 153 |
| BcnI | CCSGG | 1 cut(s) | 443 |
| BcoDI | GTCTC | 1 cut(s) | 200 |
| BfaI | CTAG | 1 cut(s) | 577 |
| BfmI | CTRYAG | 1 cut(s) | 172 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 4 cut(s) | 157, 270, 564, 567 |
| BlsI | GCNGC | 4 cut(s) | 158, 271, 565, 568 |
| Bme1390I | CCNGG | 2 cut(s) | 153, 443 |
| BmgT120I | GGNCC | 1 cut(s) | 439 |
| BmrFI | CCNGG | 2 cut(s) | 153, 443 |
| BmsI | GCATC | 2 cut(s) | 337, 500 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 381, 413 |
| Bpu14I | TTCGAA | 1 cut(s) | 28 |
| BpuMI | CCSGG | 1 cut(s) | 443 |
| BsaHI | GRCGYC | 1 cut(s) | 329 |
| BsaJI | CCNNGG | 2 cut(s) | 142, 324 |
| BsaWI | WCCGGW | 1 cut(s) | 401 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 30, 34, 60 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 325 |
| Bse118I | RCCGGY | 1 cut(s) | 341 |
| BseBI | CCWGG | 1 cut(s) | 153 |
| BseDI | CCNNGG | 2 cut(s) | 142, 324 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 325 |
| BseRI | GAGGAG | 1 cut(s) | 116 |
| BseXI | GCAGC | 1 cut(s) | 575 |
| Bsh1285I | CGRYCG | 1 cut(s) | 253 |
| BshFI | GGCC | 4 cut(s) | 147, 156, 318, 441 |
| BsiEI | CGRYCG | 1 cut(s) | 253 |
| BsiSI | CCGG | 4 cut(s) | 273, 342, 402, 442 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 325 |
| BsmAI | GTCTC | 1 cut(s) | 200 |
| BsmBI | CGTCTC | 1 cut(s) | 200 |
| BsnI | GGCC | 4 cut(s) | 147, 156, 318, 441 |
| Bsp119I | TTCGAA | 1 cut(s) | 28 |
| Bsp143I | GATC | 1 cut(s) | 384 |
| BspACI | CCGC | 8 cut(s) | 23, 41, 133, 157, 162, 253, 267, 270 |
| BspANI | GGCC | 4 cut(s) | 147, 156, 318, 441 |
| BspT104I | TTCGAA | 1 cut(s) | 28 |
| BsrBI | CCGCTC | 1 cut(s) | 159 |
| BsrFI | RCCGGY | 1 cut(s) | 341 |
| BssAI | RCCGGY | 1 cut(s) | 341 |
| BssECI | CCNNGG | 2 cut(s) | 142, 324 |
| BssMI | GATC | 1 cut(s) | 384 |
| BssNI | GRCGYC | 1 cut(s) | 329 |
| BssT1I | CCWWGG | 2 cut(s) | 142, 324 |
| Bst2UI | CCWGG | 1 cut(s) | 153 |
| Bst6I | CTCTTC | 3 cut(s) | 70, 77, 491 |
| BstACI | GRCGYC | 1 cut(s) | 329 |
| BstBI | TTCGAA | 1 cut(s) | 28 |
| BstC8I | GCNNGC | 1 cut(s) | 320 |
| BstEII | GGTNACC | 1 cut(s) | 445 |
| BstKTI | GATC | 1 cut(s) | 387 |
| BstMAI | GTCTC | 1 cut(s) | 200 |
| BstMBI | GATC | 1 cut(s) | 384 |
| BstMCI | CGRYCG | 1 cut(s) | 253 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 31, 40, 153, 261, 347, 356 |
| BstNI | CCWGG | 1 cut(s) | 153 |
| BstPI | GGTNACC | 1 cut(s) | 445 |
| BstSCI | CCNGG | 2 cut(s) | 151, 441 |
| BstSFI | CTRYAG | 1 cut(s) | 172 |
| BstV1I | GCAGC | 1 cut(s) | 575 |
| BsuRI | GGCC | 4 cut(s) | 147, 156, 318, 441 |
| Cac8I | GCNNGC | 1 cut(s) | 320 |
| Cfr10I | RCCGGY | 1 cut(s) | 341 |
| Cfr13I | GGNCC | 1 cut(s) | 439 |
| CseI | GACGC | 1 cut(s) | 318 |
| Csp6I | GTAC | 1 cut(s) | 571 |
| CviAII | CATG | 1 cut(s) | 119 |
| CviQI | GTAC | 1 cut(s) | 571 |
| DpnI | GATC | 1 cut(s) | 386 |
| DpnII | GATC | 1 cut(s) | 384 |
| DraIII | CACNNNGTG | 1 cut(s) | 415 |
| EaeI | YGGCCR | 2 cut(s) | 154, 316 |
| Eam1104I | CTCTTC | 3 cut(s) | 70, 77, 491 |
| EarI | CTCTTC | 3 cut(s) | 70, 77, 491 |
| EciI | GGCGGA | 2 cut(s) | 151, 256 |
| Eco130I | CCWWGG | 2 cut(s) | 142, 324 |
| Eco57I | CTGAAG | 1 cut(s) | 401 |
| Eco91I | GGTNACC | 1 cut(s) | 445 |
| EcoO65I | GGTNACC | 1 cut(s) | 445 |
| EcoRII | CCWGG | 1 cut(s) | 151 |
| EcoT14I | CCWWGG | 2 cut(s) | 142, 324 |
| ErhI | CCWWGG | 2 cut(s) | 142, 324 |
| Esp3I | CGTCTC | 1 cut(s) | 200 |
| FaeI | CATG | 1 cut(s) | 122 |
| FaiI | YATR | 6 cut(s) | 11, 120, 464, 479, 507, 543 |
| FatI | CATG | 1 cut(s) | 118 |
| FblI | GTMKAC | 2 cut(s) | 171, 249 |
| Fnu4HI | GCNGC | 4 cut(s) | 157, 270, 564, 567 |
| Fsp4HI | GCNGC | 4 cut(s) | 157, 270, 564, 567 |
| FspBI | CTAG | 1 cut(s) | 577 |
| GluI | GCNGC | 4 cut(s) | 157, 270, 564, 567 |
| HaeIII | GGCC | 4 cut(s) | 147, 156, 318, 441 |
| HapII | CCGG | 4 cut(s) | 273, 342, 402, 442 |
| HgaI | GACGC | 1 cut(s) | 318 |
| Hin1I | GRCGYC | 1 cut(s) | 329 |
| Hin1II | CATG | 1 cut(s) | 122 |
| HincII | GTYRAC | 1 cut(s) | 250 |
| HindII | GTYRAC | 1 cut(s) | 250 |
| HinfI | GANTC | 1 cut(s) | 376 |
| HpaII | CCGG | 4 cut(s) | 273, 342, 402, 442 |
| HphI | GGTGA | 3 cut(s) | 356, 446, 457 |
| Hpy166II | GTNNAC | 3 cut(s) | 172, 250, 407 |
| Hpy188I | TCNGA | 2 cut(s) | 381, 389 |
| Hpy188III | TCNNGA | 2 cut(s) | 197, 281 |
| Hpy8I | GTNNAC | 3 cut(s) | 172, 250, 407 |
| Hpy99I | CGWCG | 1 cut(s) | 203 |
| HpyAV | CCTTC | 2 cut(s) | 218, 247 |
| HpyCH4IV | ACGT | 1 cut(s) | 201 |
| HpyCH4V | TGCA | 1 cut(s) | 563 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 31, 40, 153, 261, 347, 356 |
| HpySE526I | ACGT | 1 cut(s) | 201 |
| Hsp92I | GRCGYC | 1 cut(s) | 329 |
| Hsp92II | CATG | 1 cut(s) | 122 |
| Kzo9I | GATC | 1 cut(s) | 384 |
| LmnI | GCTCC | 2 cut(s) | 164, 269 |
| Lsp1109I | GCAGC | 1 cut(s) | 575 |
| LweI | GCATC | 2 cut(s) | 337, 500 |
| MaeI | CTAG | 1 cut(s) | 577 |
| MaeII | ACGT | 1 cut(s) | 201 |
| MaeIII | GTNAC | 2 cut(s) | 330, 445 |
| MalI | GATC | 1 cut(s) | 386 |
| MbiI | CCGCTC | 1 cut(s) | 159 |
| MboI | GATC | 1 cut(s) | 384 |
| MboII | GAAGA | 5 cut(s) | 57, 64, 67, 394, 508 |
| MluCI | AATT | 2 cut(s) | 508, 538 |
| MlyI | GAGTC | 1 cut(s) | 385 |
| MnlI | CCTC | 9 cut(s) | 73, 92, 134, 137, 175, 224, 229, 236, 430 |
| MspI | CCGG | 4 cut(s) | 273, 342, 402, 442 |
| MspR9I | CCNGG | 2 cut(s) | 153, 443 |
| MvaI | CCWGG | 1 cut(s) | 153 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 31, 40, 153, 261, 347, 356 |
| NciI | CCSGG | 1 cut(s) | 443 |
| NdeII | GATC | 1 cut(s) | 384 |
| NlaIII | CATG | 1 cut(s) | 122 |
| NmuCI | GTSAC | 2 cut(s) | 330, 445 |
| NspV | TTCGAA | 1 cut(s) | 28 |
| PflFI | GACNNNGTC | 1 cut(s) | 119 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 325 |
| PkrI | GCNGC | 4 cut(s) | 158, 271, 565, 568 |
| PleI | GAGTC | 1 cut(s) | 384 |
| PpsI | GAGTC | 1 cut(s) | 384 |
| Psp6I | CCWGG | 1 cut(s) | 151 |
| PspEI | GGTNACC | 1 cut(s) | 445 |
| PspGI | CCWGG | 1 cut(s) | 151 |
| PspPI | GGNCC | 1 cut(s) | 439 |
| PsyI | GACNNNGTC | 1 cut(s) | 119 |
| RsaI | GTAC | 1 cut(s) | 572 |
| RsaNI | GTAC | 1 cut(s) | 571 |
| SalI | GTCGAC | 1 cut(s) | 248 |
| SatI | GCNGC | 4 cut(s) | 157, 270, 564, 567 |
| Sau3AI | GATC | 1 cut(s) | 384 |
| Sau96I | GGNCC | 1 cut(s) | 439 |
| SchI | GAGTC | 1 cut(s) | 385 |
| ScrFI | CCNGG | 2 cut(s) | 153, 443 |
| SetI | ASST | 5 cut(s) | 65, 204, 266, 312, 452 |
| SfaNI | GCATC | 2 cut(s) | 337, 500 |
| SfcI | CTRYAG | 1 cut(s) | 172 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 153 |
| SfuI | TTCGAA | 1 cut(s) | 28 |
| SgrAI | CRCCGGYG | 1 cut(s) | 341 |
| Sse9I | AATT | 2 cut(s) | 508, 538 |
| SsiI | CCGC | 8 cut(s) | 23, 41, 133, 157, 162, 253, 267, 270 |
| SspMI | CTAG | 1 cut(s) | 577 |
| StyD4I | CCNGG | 2 cut(s) | 151, 441 |
| StyI | CCWWGG | 2 cut(s) | 142, 324 |
| TaiI | ACGT | 1 cut(s) | 204 |
| TaqI | TCGA | 4 cut(s) | 28, 198, 249, 494 |
| TasI | AATT | 2 cut(s) | 508, 538 |
| TauI | GCSGC | 2 cut(s) | 159, 272 |
| TseFI | GTSAC | 2 cut(s) | 330, 445 |
| TseI | GCWGC | 2 cut(s) | 563, 566 |
| Tsp45I | GTSAC | 2 cut(s) | 330, 445 |
| TspDTI | ATGAA | 1 cut(s) | 342 |
| TspGWI | ACGGA | 3 cut(s) | 157, 211, 232 |
| Tth111I | GACNNNGTC | 1 cut(s) | 119 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 325 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 149 |
| XmiI | GTMKAC | 2 cut(s) | 171, 249 |
| XspI | CTAG | 1 cut(s) | 577 |