MD09G1149300.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014476
Physical Location & Seq
Forward (+)
11730939 .. 11732612
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1149300.v1.1.491

Sequence Viewer

Length: 1674 bp
ATGGCTGTTAAGGCTTCAATCCTACTGGTTCTCCTTGTAATCTTCTCCTCCACTTTAGCAGCAATTGCCAGAGTTCACAATCACCGCAATCACAACTCAAATGCCTCCACTTTCGCCGGCATACAACTGCCGAAGCACATGAGCTTCAACGCCGTCTCCTTCTCATCCCACACCGGCTGCAGCCTCAGCAGCTCCAAAATACCCAACCAACCAGATTCAGCCATGGAAGAAGCTGAAGACTCCGACACCGACGAAGCAGCACCCGAATTGAACCCCCATAAACAGACCATGAAGCTCCACCTACGGCACAGATCACAGAACAAACAATCCGAAAGAAAAAGTTCAGTGATCGAGTCCACAGTCCGAGACTTGGTCAGAATTCAAACCCTCCACACAAGAATCGTCGAGAAGAAGAATCAGAACACATTTTCAAGGCTGCAGAAAGATAAGAAACCGAAGCACCACCAATCAAATCCGGTTGTAGCCCCAGCAGCCTCGCCGGAGTCCTACACCAACGAGCTCTCTGGCCAGCTTCAGGCCACGTTGAAGTCCGGCGTCAGCCTCGGTTCCGGCGAGTACTTCATGGATGTTTTCATCGGTACACCCCCTAAACACTTCTCTTTAATTCTCGACACTGGCAGTGACCTTAACTGGGTCCAATGCGCGCCGTGCCATGACTGCTTCGAGCAGCACGGTCCGCATTACGACCCCAAAGACTCAACTTCTTTTCTTGACATTAGCTGCAAGGATCCGCGGTGTCGATTAGTTTCGTCCCCGGATCCTCCGCAGCCCTGCAAGTCCGAAAATCAAACGTGTCCGTATTTCTACTGGTACGGAGACAGCTCCAACACCACCGGCGACTTCGCCCTCGAAACCTTTACCATCAACCTCACGTCATCGAACAAGGCGGAGTTCAAGAGGGTGGAGAATGTGATGTTTGGATGCGGCCATTGGAACAGAGGATTGTTCCATGGGGCTGCAGGGTTGCTAGGTCTTGGCAGAGGTCCTCTTTCCTTTGCTTCTCAGCTCCAATCGCTGTACGGCCACTCGTTTTCCTACTGCCTTGTGGATCGAAACAGCGATGCGAATGTCAGCAGCAAGTTGATTTTCGGGGAGGACAAGAACCTCTTGAGCCACCCGAAATTGACCTACACTTCCTTGGTGGGAGGGAAAGAAAACCCTGCTGATACATTCTACTATGTCGAGATAAAATCCATCATGGTCGGCGGAGAAGCGGTGGACATACCGGCTGAGACTTGGAAATTGTCGCCGGAGGGTGCGGGTGGGACAATCGTTGATTCCGGCACCACGTTGAGTTACTTTGCTGATCCAGCCTATCAAATTATCAAGGACGCTTTTGAGAAAAAGGTTAAAGGGTATCCAGTAGTGAATGACTTTCCATTTTTGGAACCTTGTTACAATGTGTCAGGTGTTGAGAAAATAGAGCTGCCGGAATTTGCGATTGTGTTTGCGGACGGAGCTGTGTGGGATTTCCCAGTTGAGAACTACTTCATCCAGATCGAACCGCAGGAAATCGTCTGCCTGGCGGTGTTGGGGACTCCGAAATCCGGCCTTTCGATCATCGGTAACTATCAGCAGCAGAATTTTCATATCCTCTATGATACAAAGAAGTCTAGGTTAGGATATGCACCCATGAAATGTGCTGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18A TAXi_C TAXi_N

Protein Analysis

558

Amino Acids

61.53

Weight (kDa)

6.13

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 192 - 551 4.8e-22 Eukaryotic aspartyl protease
TAXi_N PF14543 193 - 371 6.2e-53 Xylanase inhibitor N-terminal
TAXi_C PF14541 399 - 550 2e-35 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59080 AT3G59080
fragaria_vesca FvH4_6g38620
malus_domestica MD09G1149300.v1.1 MD17G1135600.v1.1
prunus_persica Prupe.3G177100_v2.0.a1
pyrus_communis pycom09g06860 pycom17g12940
rosa_chinensis RchiOBHm_Chr2g0152031
rosa_laevigata RLG00000020602
rosa_roxburghii Rroxscaffold_2G00096030
rosa_rugosa Rorug02G0430400
rosa_samantha Rh2AG491200 Rh2BG503500 Rh2CG477600 Rh2DG514800
rosa_wichuraiana Rw2G040380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1304
AccII CGCG 2 cut(s) 665, 754
AclWI GGATC 6 cut(s) 743, 756, 773, 786, 1077, 1322
AcoI YGGCCR 3 cut(s) 526, 946, 1042
AcsI RAATTY 3 cut(s) 378, 1454, 1603
AcuI CTGAAG 2 cut(s) 255, 518
AcyI GRCGYC 1 cut(s) 555
AfaI GTAC 4 cut(s) 578, 601, 833, 1040
AfiI CCNNNNNNNGG 4 cut(s) 370, 535, 652, 1568
AflIII ACRYGT 1 cut(s) 812
AgsI TTSAA 7 cut(s) 18, 148, 271, 383, 432, 547, 916
AjiI CACGTC 1 cut(s) 894
AjnI CCWGG 1 cut(s) 1542
AloI GAACNNNNNNTCC 2 cut(s) 311, 343
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 4 cut(s) 160, 360, 831, 1247
AlwI GGATC 6 cut(s) 743, 756, 773, 786, 1077, 1322
AoxI GGCC 5 cut(s) 526, 537, 946, 1042, 1570
ApoI RAATTY 3 cut(s) 378, 1454, 1603
Asp700I GAANNNNTTC 2 cut(s) 340, 1508
AspLEI GCGC 2 cut(s) 665, 667
AspS9I GGNCC 3 cut(s) 655, 695, 1004
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 3 cut(s) 655, 695, 1004
BalI TGGCCA 1 cut(s) 528
BamHI GGATCC 2 cut(s) 748, 778
BanI GGYRCC 1 cut(s) 1304
BanII GRGCYC 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 522
BbvCI CCTCAGC 1 cut(s) 185
BccI CCATC 2 cut(s) 890, 1223
BceAI ACGGC 4 cut(s) 137, 320, 652, 1057
BciT130I CCWGG 1 cut(s) 1544
BciVI GTATCC 1 cut(s) 1389
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 4 cut(s) 160, 360, 831, 1247
BfaI CTAG 2 cut(s) 989, 1635
BfmI CTRYAG 3 cut(s) 178, 437, 978
BfuI GTATCC 1 cut(s) 1389
BmcAI AGTACT 1 cut(s) 578
Bme1390I CCNGG 2 cut(s) 776, 1544
Bme18I GGWCC 3 cut(s) 655, 695, 1004
BmgBI CACGTC 1 cut(s) 894
BmgT120I GGNCC 3 cut(s) 655, 695, 1004
BmiI GGNNCC 6 cut(s) 568, 656, 750, 780, 1306, 1410
BmrFI CCNGG 2 cut(s) 776, 1544
BmrI ACTGGG 2 cut(s) 661, 1490
BmsI GCATC 2 cut(s) 932, 1072
BmuI ACTGGG 2 cut(s) 661, 1490
BpiI GAAGAC 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 1150
BpuMI CCSGG 1 cut(s) 776
BsaHI GRCGYC 1 cut(s) 555
BsaJI CCNNGG 6 cut(s) 222, 562, 752, 774, 970, 1158
BsaWI WCCGGW 1 cut(s) 475
BsaXI ACNNNNNCTCC 6 cut(s) 15, 45, 140, 170, 1221, 1251
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 535, 652, 1568
Bse118I RCCGGY 4 cut(s) 116, 173, 854, 1246
Bse1I ACTGG 6 cut(s) 30, 640, 656, 833, 1382, 1496
BseBI CCWGG 1 cut(s) 1544
BseDI CCNNGG 6 cut(s) 222, 562, 752, 774, 970, 1158
BseGI GGATG 4 cut(s) 164, 592, 947, 1512
BseLI CCNNNNNNNGG 4 cut(s) 370, 535, 652, 1568
BseMII CTCAG 3 cut(s) 199, 1037, 1242
BseNI ACTGG 6 cut(s) 30, 640, 656, 833, 1382, 1496
BsePI GCGCGC 1 cut(s) 663
BseRI GAGGAG 1 cut(s) 37
BseYI CCCAGC 1 cut(s) 487
Bsh1236I CGCG 2 cut(s) 665, 754
BshFI GGCC 5 cut(s) 528, 539, 948, 1044, 1572
BshNI GGYRCC 1 cut(s) 1304
BsiHKAI GWGCWC 1 cut(s) 522
BslFI GGGAC 3 cut(s) 757, 1300, 1570
BslI CCNNNNNNNGG 4 cut(s) 370, 535, 652, 1568
BsmAI GTCTC 4 cut(s) 160, 360, 831, 1247
BsmBI CGTCTC 1 cut(s) 160
BsmFI GGGAC 3 cut(s) 757, 1300, 1570
BsnI GGCC 5 cut(s) 528, 539, 948, 1044, 1572
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
Bsp19I CCATGG 2 cut(s) 222, 970
BspANI GGCC 5 cut(s) 528, 539, 948, 1044, 1572
BspCNI CTCAG 3 cut(s) 198, 1036, 1243
BspFNI CGCG 2 cut(s) 665, 754
BspLI GGNNCC 6 cut(s) 568, 656, 750, 780, 1306, 1410
BspMAI CTGCAG 3 cut(s) 182, 441, 982
BspPI GGATC 6 cut(s) 743, 756, 773, 786, 1077, 1322
BspT107I GGYRCC 1 cut(s) 1304
BsrFI RCCGGY 4 cut(s) 116, 173, 854, 1246
BsrI ACTGG 6 cut(s) 30, 640, 656, 833, 1382, 1496
BssAI RCCGGY 4 cut(s) 116, 173, 854, 1246
BssECI CCNNGG 6 cut(s) 222, 562, 752, 774, 970, 1158
BssHII GCGCGC 1 cut(s) 663
BssMI GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
BssNI GRCGYC 1 cut(s) 555
BssT1I CCWWGG 3 cut(s) 222, 970, 1158
Bst2UI CCWGG 1 cut(s) 1544
Bst4CI ACNGT 2 cut(s) 361, 695
BstACI GRCGYC 1 cut(s) 555
BstAPI GCANNNNNTGC 1 cut(s) 65
BstC8I GCNNGC 3 cut(s) 118, 530, 665
BstDEI CTNAG 3 cut(s) 185, 1023, 1251
BstDSI CCRYGG 3 cut(s) 222, 752, 970
BstF5I GGATG 4 cut(s) 164, 592, 947, 1512
BstFNI CGCG 2 cut(s) 665, 754
BstHHI GCGC 2 cut(s) 665, 667
BstKTI GATC 8 cut(s) 314, 351, 751, 781, 1072, 1330, 1521, 1581
BstMAI GTCTC 4 cut(s) 160, 360, 831, 1247
BstMBI GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
BstNI CCWGG 1 cut(s) 1544
BstSCI CCNGG 2 cut(s) 774, 1542
BstSFI CTRYAG 3 cut(s) 178, 437, 978
BstUI CGCG 2 cut(s) 665, 754
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 2 cut(s) 748, 778
BstYI RGATCY 2 cut(s) 748, 778
BsuI GTATCC 1 cut(s) 1389
BsuRI GGCC 5 cut(s) 528, 539, 948, 1044, 1572
BtgI CCRYGG 3 cut(s) 222, 752, 970
BtgZI GCGATG 1 cut(s) 1095
BtrI CACGTC 1 cut(s) 894
BtsCI GGATG 4 cut(s) 164, 592, 947, 1512
BtsI GCAGTG 1 cut(s) 646
BtsIMutI CAGTG 3 cut(s) 351, 633, 646
Cac8I GCNNGC 3 cut(s) 118, 530, 665
CfoI GCGC 2 cut(s) 665, 667
Cfr10I RCCGGY 4 cut(s) 116, 173, 854, 1246
Cfr13I GGNCC 3 cut(s) 655, 695, 1004
Cfr42I CCGCGG 1 cut(s) 755
CpoI CGGWCCG 1 cut(s) 695
CseI GACGC 2 cut(s) 544, 1361
Csp6I GTAC 4 cut(s) 577, 600, 832, 1039
CspI CGGWCCG 1 cut(s) 695
CviAII CATG 8 cut(s) 139, 223, 289, 583, 674, 971, 1219, 1654
CviQI GTAC 4 cut(s) 577, 600, 832, 1039
DdeI CTNAG 3 cut(s) 185, 1023, 1251
DpnI GATC 8 cut(s) 313, 350, 750, 780, 1071, 1329, 1520, 1580
DpnII GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
EaeI YGGCCR 3 cut(s) 526, 946, 1042
EciI GGCGGA 2 cut(s) 923, 1242
Ecl136II GAGCTC 1 cut(s) 520
Eco130I CCWWGG 3 cut(s) 222, 970, 1158
Eco24I GRGCYC 1 cut(s) 522
Eco47I GGWCC 3 cut(s) 655, 695, 1004
Eco53kI GAGCTC 1 cut(s) 520
Eco57I CTGAAG 2 cut(s) 255, 518
EcoICRI GAGCTC 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 1004
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 1542
EcoT14I CCWWGG 3 cut(s) 222, 970, 1158
EcoT38I GRGCYC 1 cut(s) 522
ErhI CCWWGG 3 cut(s) 222, 970, 1158
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 8 cut(s) 142, 226, 292, 586, 677, 974, 1222, 1657
FalI AAGNNNNNCTT 2 cut(s) 1112, 1144
FaqI GGGAC 3 cut(s) 757, 1300, 1570
FatI CATG 8 cut(s) 138, 222, 288, 582, 673, 970, 1218, 1653
FauI CCCGC 1 cut(s) 1273
FokI GGATG 4 cut(s) 151, 599, 954, 1499
FriOI GRGCYC 1 cut(s) 522
FspBI CTAG 2 cut(s) 989, 1635
GlaI GCGC 2 cut(s) 664, 666
GsaI CCCAGC 1 cut(s) 491
HaeIII GGCC 5 cut(s) 528, 539, 948, 1044, 1572
HgaI GACGC 2 cut(s) 544, 1361
HhaI GCGC 2 cut(s) 665, 667
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 8 cut(s) 142, 226, 292, 586, 677, 974, 1222, 1657
Hin6I GCGC 2 cut(s) 663, 665
HinP1I GCGC 2 cut(s) 663, 665
HinfI GANTC 9 cut(s) 215, 239, 353, 399, 415, 503, 716, 1298, 1558
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 4 cut(s) 76, 357, 602, 1240
Hpy188I TCNGA 7 cut(s) 244, 331, 365, 377, 420, 802, 1563
Hpy188III TCNNGA 7 cut(s) 406, 629, 731, 916, 1129, 1204, 1516
Hpy8I GTNNAC 4 cut(s) 76, 357, 602, 1240
Hpy99I CGWCG 2 cut(s) 254, 407
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 2 cut(s) 361, 695
HpyCH4IV ACGT 4 cut(s) 542, 812, 893, 1310
HpyCH4V TGCA 6 cut(s) 180, 439, 744, 795, 980, 1649
HpyF3I CTNAG 3 cut(s) 185, 1023, 1251
HpySE526I ACGT 4 cut(s) 542, 812, 893, 1310
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 8 cut(s) 142, 226, 292, 586, 677, 974, 1222, 1657
HspAI GCGC 2 cut(s) 663, 665
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
KspI CCGCGG 1 cut(s) 755
Kzo9I GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
LmnI GCTCC 5 cut(s) 197, 300, 848, 1032, 1478
LweI GCATC 2 cut(s) 932, 1072
MaeI CTAG 2 cut(s) 989, 1635
MaeII ACGT 4 cut(s) 542, 812, 893, 1310
MaeIII GTNAC 4 cut(s) 641, 1316, 1415, 1586
MalI GATC 8 cut(s) 313, 350, 750, 780, 1071, 1329, 1520, 1580
MboI GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
MboII GAAGA 5 cut(s) 34, 239, 248, 421, 424
MfeI CAATTG 1 cut(s) 63
MflI RGATCY 2 cut(s) 748, 778
MhlI GDGCHC 1 cut(s) 522
MlsI TGGCCA 1 cut(s) 528
MluCI AATT 9 cut(s) 63, 266, 378, 624, 1142, 1262, 1341, 1454, 1603
MluNI TGGCCA 1 cut(s) 528
MlyI GAGTC 5 cut(s) 233, 362, 512, 710, 1552
MmeI TCCRAC 2 cut(s) 267, 870
Mox20I TGGCCA 1 cut(s) 528
MroNI GCCGGC 1 cut(s) 116
MroXI GAANNNNTTC 2 cut(s) 340, 1508
MscI TGGCCA 1 cut(s) 528
MseI TTAA 4 cut(s) 9, 623, 648, 1371
Msp20I TGGCCA 1 cut(s) 528
MspA1I CMGCKG 1 cut(s) 754
MspR9I CCNGG 2 cut(s) 776, 1544
MunI CAATTG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 1544
MvnI CGCG 2 cut(s) 665, 754
NaeI GCCGGC 1 cut(s) 118
NciI CCSGG 1 cut(s) 776
NcoI CCATGG 2 cut(s) 222, 970
NdeII GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 8 cut(s) 142, 226, 292, 586, 677, 974, 1222, 1657
NlaIV GGNNCC 6 cut(s) 568, 656, 750, 780, 1306, 1410
NmuCI GTSAC 1 cut(s) 641
PauI GCGCGC 1 cut(s) 663
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PdiI GCCGGC 1 cut(s) 118
PdmI GAANNNNTTC 2 cut(s) 340, 1508
PfeI GAWTC 4 cut(s) 215, 399, 415, 1298
PflFI GACNNNGTC 1 cut(s) 371
PleI GAGTC 5 cut(s) 233, 361, 511, 710, 1552
PpsI GAGTC 5 cut(s) 233, 361, 511, 710, 1552
PpuMI RGGWCCY 1 cut(s) 1004
Psp124BI GAGCTC 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 1004
Psp6I CCWGG 1 cut(s) 1542
PspFI CCCAGC 1 cut(s) 487
PspGI CCWGG 1 cut(s) 1542
PspN4I GGNNCC 6 cut(s) 568, 656, 750, 780, 1306, 1410
PspPI GGNCC 3 cut(s) 655, 695, 1004
PspPPI RGGWCCY 1 cut(s) 1004
PstI CTGCAG 3 cut(s) 182, 441, 982
PsuI RGATCY 2 cut(s) 748, 778
PsyI GACNNNGTC 1 cut(s) 371
PteI GCGCGC 1 cut(s) 663
RsaI GTAC 4 cut(s) 578, 601, 833, 1040
RsaNI GTAC 4 cut(s) 577, 600, 832, 1039
Rsr2I CGGWCCG 1 cut(s) 695
RsrII CGGWCCG 1 cut(s) 695
SacI GAGCTC 1 cut(s) 522
SacII CCGCGG 1 cut(s) 755
SaqAI TTAA 4 cut(s) 9, 623, 648, 1371
Sau3AI GATC 8 cut(s) 311, 348, 748, 778, 1069, 1327, 1518, 1578
Sau96I GGNCC 3 cut(s) 655, 695, 1004
ScaI AGTACT 1 cut(s) 578
SchI GAGTC 5 cut(s) 233, 362, 512, 710, 1552
ScrFI CCNGG 2 cut(s) 776, 1544
SduI GDGCHC 1 cut(s) 522
SfaNI GCATC 2 cut(s) 932, 1072
SfcI CTRYAG 3 cut(s) 178, 437, 978
Sfr303I CCGCGG 1 cut(s) 755
SgrAI CRCCGGYG 1 cut(s) 854
SgrBI CCGCGG 1 cut(s) 755
SinI GGWCC 3 cut(s) 655, 695, 1004
SmlI CTYRAG 1 cut(s) 1129
SmoI CTYRAG 1 cut(s) 1129
Sse9I AATT 9 cut(s) 63, 266, 378, 624, 1142, 1262, 1341, 1454, 1603
SspMI CTAG 2 cut(s) 989, 1635
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 774, 1542
StyI CCWWGG 3 cut(s) 222, 970, 1158
TaaI ACNGT 2 cut(s) 361, 695
TaiI ACGT 4 cut(s) 545, 815, 896, 1313
TasI AATT 9 cut(s) 63, 266, 378, 624, 1142, 1262, 1341, 1454, 1603
TatI WGTACW 1 cut(s) 576
TauI GCSGC 1 cut(s) 948
TfiI GAWTC 4 cut(s) 215, 399, 415, 1298
Tru1I TTAA 4 cut(s) 9, 623, 648, 1371
Tru9I TTAA 4 cut(s) 9, 623, 648, 1371
TscAI CASTG 3 cut(s) 351, 640, 646
TseFI GTSAC 1 cut(s) 641
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 6 cut(s) 305, 571, 583, 1501, 1598, 1670
TspGWI ACGGA 3 cut(s) 807, 849, 1491
TspRI CASTG 3 cut(s) 351, 640, 646
Tth111I GACNNNGTC 1 cut(s) 371
VpaK11BI GGWCC 3 cut(s) 655, 695, 1004
XapI RAATTY 3 cut(s) 378, 1454, 1603
XmnI GAANNNNTTC 2 cut(s) 340, 1508
XspI CTAG 2 cut(s) 989, 1635
ZrmI AGTACT 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.