MD17G1135600.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014476
Physical Location & Seq
Forward (+)
12144781 .. 12146457
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1135600.v1.1.491

Sequence Viewer

Length: 1677 bp
ATGGCTGTTAGGGGTTCAATCCTAGTGGTTCTCCTTGTAATCTTCTCCTCCACTTTAGCAGCGATTGCCGGACTCCACAGCCACGACAATCACAACCCAAATGCCTCCACTCTCGCCGGCATACAGCTGCCGGAGCACATGAGCTTCAACGCCGTCTCCTCCTCCTCATCCCACACTGGCTGCAGCCTCAGCAGCTCTAAAAGAACAAACCAATCAGATTCAGCCATGGAAGATGCTGACGACTCTGACGACGACGAAGCCGCACCCGAATTGAACCCTCATAAACAAACTATGAAGCTCCATCTACGGCACAGATCACAGAACAAACAATCCGAAAGAAAAAACTCCGTGATCGAGTCCACAGTCCGAGACTTGATCAGAATTCAAACCCTCCACACAAGAATCGTAGAGAAAAAGAACCAGAACACAATTTCAAGGCTGCAGAAAGACAAGAAGCCGAAACCCTACCAAACAAATCCGGTTGTAGCCCCTGCCGCCTCGCCGGAGTCTTACACCAACGAGCTTTCAGGGCAACTTCAGGCCACGTTGAAGTCTGGTGTCAGCCTCGGCTCCGGCGAGTACTTCATGGATGTTTTCATCGGTACACCCCCTAAACACTTCTCTTTAATTCTCGACACTGGCAGTGACCTTAACTGGGTCCAATGTGCGCCGTGCTACGACTGCTTCGAGCAGCAGGGCCCGCATTACGACCCGAAAGACTCAACTTCTTTTCTTGACATTAGCTGCAAGGATCCGCGGTGTCAATTGATTTCGTCCCCGGATCCTCCGCAGCCCTGCAAGTCCGAAAATCAAACGTGCCCGTATTTCTACTGGTACGGAGACAGCTCCAACACTACCGGCGATTTCTCCCTCGAAACCTTTACCGTGAACCTCACGTCACCGAACAAGGCAGAGTTCAAGAGGGCGGAGAATGTGATGTTCGGATGCGGACATTGGAACAGAGGACTATTTCATGGGGCTGCAGGGTTGCTAGGGCTTGGCAGAGGCCCTCTTTCCTTTGCATCTCAGCTCCAATCGCTGTACGGACATTCGTTTTCCTACTGCCTTGTGGATCGAAACAGCGACGCGAATGTCAGCAGCAAGTTGATTTTCGGGGAGGACAAGAACCTCCTGAGCCACCCGAAATTGAGCTACACTTCCTTGGTGGGAGGGAAAGAAAACCCTGCTGATACATTCTACTATGTGGAGATAAAATCAGTCATGGTCGGCGGAGAAGCGGTGGACATACCGGCTGAGACTTGGAAATTGTCGCCGAAGGGTGCCGGCGGGACAATCATTGATTCCGGCACCACGTTGAGTTACTTTGCCGATCCTGCTTACCAAATTATCAAGGAGGCATTTGAGAAAAAGGTTAAAGGGTATCCAGTTGTGAAGGACTTTCCAATTTTGGAACCTTGTTACAATGTGTCAGGTGTCGAGAAAATAGAGCTGCCGGAGTTCGCAATTGTGTTTGCGGATGGAGCTGTGTGGGATTTCCCAGTAGAGAACTACTTCATCCAGATCGAACCACAGGAAATGGTCTGCCTGGCGGTGTTGGGGACTCCGAAATCCGGCCTTTCAATCATCGGAAACTATCAGCAGCAGAATTTTCATATCCTCTATGACACAAAGAAGTCTAGGCTAGGATATGCACCCATGAAATGTGCTGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18A TAXi_C TAXi_N

Protein Analysis

559

Amino Acids

61.4

Weight (kDa)

5.75

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 193 - 552 6.1e-22 Eukaryotic aspartyl protease
TAXi_N PF14543 194 - 372 4.2e-52 Xylanase inhibitor N-terminal
TAXi_C PF14541 400 - 551 2.2e-35 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59080 AT3G59080
fragaria_vesca FvH4_6g38620
malus_domestica MD09G1149300.v1.1 MD17G1135600.v1.1
prunus_persica Prupe.3G177100_v2.0.a1
pyrus_communis pycom09g06860 pycom17g12940
rosa_chinensis RchiOBHm_Chr2g0152031
rosa_laevigata RLG00000020602
rosa_roxburghii Rroxscaffold_2G00096030
rosa_rugosa Rorug02G0430400
rosa_samantha Rh2AG491200 Rh2BG503500 Rh2CG477600 Rh2DG514800
rosa_wichuraiana Rw2G040380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1091
AccB1I GGYRCC 2 cut(s) 1280, 1307
AccII CGCG 2 cut(s) 757, 1088
AclWI GGATC 6 cut(s) 746, 759, 776, 789, 1080, 1325
AcsI RAATTY 2 cut(s) 381, 1606
AcuI CTGAAG 1 cut(s) 521
AfaI GTAC 4 cut(s) 581, 604, 836, 1043
AfiI CCNNNNNNNGG 2 cut(s) 655, 1571
AgsI TTSAA 8 cut(s) 18, 148, 274, 386, 435, 550, 919, 1581
AjiI CACGTC 1 cut(s) 897
AjnI CCWGG 1 cut(s) 1545
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 4 cut(s) 160, 363, 834, 1250
AlwI GGATC 6 cut(s) 746, 759, 776, 789, 1080, 1325
AoxI GGCC 4 cut(s) 540, 697, 1006, 1573
ApaI GGGCCC 1 cut(s) 701
ApoI RAATTY 2 cut(s) 381, 1606
Asp700I GAANNNNTTC 1 cut(s) 1511
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 4 cut(s) 658, 697, 698, 1007
AsuC2I CCSGG 1 cut(s) 779
AsuHPI GGTGA 1 cut(s) 891
AvaII GGWCC 1 cut(s) 658
BaeGI GKGCMC 2 cut(s) 701, 821
BamHI GGATCC 2 cut(s) 751, 781
BanI GGYRCC 2 cut(s) 1280, 1307
BanII GRGCYC 1 cut(s) 701
Bbv12I GWGCWC 1 cut(s) 138
BbvCI CCTCAGC 1 cut(s) 188
BccI CCATC 2 cut(s) 309, 1472
BceAI ACGGC 3 cut(s) 137, 323, 655
BciT130I CCWGG 1 cut(s) 1547
BciVI GTATCC 1 cut(s) 1392
BclI TGATCA 1 cut(s) 375
BcnI CCSGG 1 cut(s) 779
BcoDI GTCTC 4 cut(s) 160, 363, 834, 1250
BfaI CTAG 4 cut(s) 23, 992, 1638, 1643
BfmI CTRYAG 3 cut(s) 181, 440, 981
BfuI GTATCC 1 cut(s) 1392
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 2 cut(s) 779, 1547
Bme18I GGWCC 1 cut(s) 658
BmgBI CACGTC 1 cut(s) 897
BmgT120I GGNCC 4 cut(s) 658, 697, 698, 1007
BmiI GGNNCC 8 cut(s) 571, 659, 699, 753, 783, 1282, 1309, 1413
BmrFI CCNGG 2 cut(s) 779, 1547
BmrI ACTGGG 2 cut(s) 664, 1493
BmsI GCATC 3 cut(s) 223, 935, 1031
BmuI ACTGGG 2 cut(s) 664, 1493
Bpu10I CCTNAGC 2 cut(s) 188, 1133
BpuMI CCSGG 1 cut(s) 779
BsaJI CCNNGG 5 cut(s) 225, 565, 755, 777, 1161
BsaWI WCCGGW 1 cut(s) 478
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 1224, 1254
Bsc4I CCNNNNNNNGG 2 cut(s) 655, 1571
Bse118I RCCGGY 4 cut(s) 116, 857, 1249, 1283
Bse1I ACTGG 6 cut(s) 181, 643, 659, 836, 1385, 1499
BseBI CCWGG 1 cut(s) 1547
BseDI CCNNGG 5 cut(s) 225, 565, 755, 777, 1161
BseGI GGATG 5 cut(s) 167, 595, 950, 1483, 1515
BseLI CCNNNNNNNGG 2 cut(s) 655, 1571
BseMII CTCAG 4 cut(s) 202, 1040, 1124, 1245
BseNI ACTGG 6 cut(s) 181, 643, 659, 836, 1385, 1499
BseRI GAGGAG 4 cut(s) 37, 148, 151, 154
BseSI GKGCMC 2 cut(s) 701, 821
Bsh1236I CGCG 2 cut(s) 757, 1088
BshFI GGCC 4 cut(s) 542, 699, 1008, 1575
BshNI GGYRCC 2 cut(s) 1280, 1307
BsiHKAI GWGCWC 1 cut(s) 138
BslFI GGGAC 3 cut(s) 760, 1303, 1573
BslI CCNNNNNNNGG 2 cut(s) 655, 1571
BsmAI GTCTC 4 cut(s) 160, 363, 834, 1250
BsmBI CGTCTC 1 cut(s) 160
BsmFI GGGAC 3 cut(s) 760, 1303, 1573
BsnI GGCC 4 cut(s) 542, 699, 1008, 1575
Bsp120I GGGCCC 1 cut(s) 697
Bsp1286I GDGCHC 3 cut(s) 138, 701, 821
Bsp143I GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
Bsp19I CCATGG 1 cut(s) 225
BspANI GGCC 4 cut(s) 542, 699, 1008, 1575
BspCNI CTCAG 4 cut(s) 201, 1039, 1125, 1246
BspFNI CGCG 2 cut(s) 757, 1088
BspLI GGNNCC 8 cut(s) 571, 659, 699, 753, 783, 1282, 1309, 1413
BspMAI CTGCAG 3 cut(s) 185, 444, 985
BspPI GGATC 6 cut(s) 746, 759, 776, 789, 1080, 1325
BspT107I GGYRCC 2 cut(s) 1280, 1307
BsrFI RCCGGY 4 cut(s) 116, 857, 1249, 1283
BsrI ACTGG 6 cut(s) 181, 643, 659, 836, 1385, 1499
BssAI RCCGGY 4 cut(s) 116, 857, 1249, 1283
BssECI CCNNGG 5 cut(s) 225, 565, 755, 777, 1161
BssMI GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
BssT1I CCWWGG 2 cut(s) 225, 1161
Bst2UI CCWGG 1 cut(s) 1547
Bst4CI ACNGT 2 cut(s) 364, 886
BstAPI GCANNNNNTGC 1 cut(s) 65
BstC8I GCNNGC 3 cut(s) 118, 701, 1285
BstDEI CTNAG 4 cut(s) 188, 1026, 1133, 1254
BstDSI CCRYGG 2 cut(s) 225, 755
BstF5I GGATG 5 cut(s) 167, 595, 950, 1483, 1515
BstFNI CGCG 2 cut(s) 757, 1088
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 8 cut(s) 317, 354, 378, 754, 784, 1075, 1333, 1524
BstMAI GTCTC 4 cut(s) 160, 363, 834, 1250
BstMBI GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
BstNI CCWGG 1 cut(s) 1547
BstSCI CCNGG 2 cut(s) 777, 1545
BstSFI CTRYAG 3 cut(s) 181, 440, 981
BstSLI GKGCMC 2 cut(s) 701, 821
BstUI CGCG 2 cut(s) 757, 1088
BstX2I RGATCY 2 cut(s) 751, 781
BstYI RGATCY 2 cut(s) 751, 781
BsuI GTATCC 1 cut(s) 1392
BsuRI GGCC 4 cut(s) 542, 699, 1008, 1575
BtgI CCRYGG 2 cut(s) 225, 755
BtrI CACGTC 1 cut(s) 897
BtsCI GGATG 5 cut(s) 167, 595, 950, 1483, 1515
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 3 cut(s) 174, 636, 649
Cac8I GCNNGC 3 cut(s) 118, 701, 1285
CfoI GCGC 1 cut(s) 670
Cfr10I RCCGGY 4 cut(s) 116, 857, 1249, 1283
Cfr13I GGNCC 4 cut(s) 658, 697, 698, 1007
Cfr42I CCGCGG 1 cut(s) 758
CseI GACGC 1 cut(s) 1094
Csp6I GTAC 4 cut(s) 580, 603, 835, 1042
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 6 cut(s) 139, 226, 586, 974, 1222, 1657
CviQI GTAC 4 cut(s) 580, 603, 835, 1042
DdeI CTNAG 4 cut(s) 188, 1026, 1133, 1254
DpnI GATC 8 cut(s) 316, 353, 377, 753, 783, 1074, 1332, 1523
DpnII GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
DrdI GACNNNNNNGTC 1 cut(s) 1091
DseDI GACNNNNNNGTC 1 cut(s) 1091
EciI GGCGGA 2 cut(s) 941, 1245
Eco130I CCWWGG 2 cut(s) 225, 1161
Eco24I GRGCYC 1 cut(s) 701
Eco47I GGWCC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 521
EcoO109I RGGNCCY 2 cut(s) 697, 1007
EcoRI GAATTC 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 1545
EcoT14I CCWWGG 2 cut(s) 225, 1161
EcoT38I GRGCYC 1 cut(s) 701
ErhI CCWWGG 2 cut(s) 225, 1161
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 6 cut(s) 142, 229, 589, 977, 1225, 1660
FaqI GGGAC 3 cut(s) 760, 1303, 1573
FatI CATG 6 cut(s) 138, 225, 585, 973, 1221, 1656
FauI CCCGC 2 cut(s) 708, 1280
FbaI TGATCA 1 cut(s) 375
FokI GGATG 5 cut(s) 154, 602, 957, 1490, 1502
FriOI GRGCYC 1 cut(s) 701
FspBI CTAG 4 cut(s) 23, 992, 1638, 1643
GlaI GCGC 1 cut(s) 669
HaeIII GGCC 4 cut(s) 542, 699, 1008, 1575
HgaI GACGC 1 cut(s) 1094
HhaI GCGC 1 cut(s) 670
Hin1II CATG 6 cut(s) 142, 229, 589, 977, 1225, 1660
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HinfI GANTC 9 cut(s) 72, 218, 242, 356, 402, 506, 719, 1301, 1561
HphI GGTGA 1 cut(s) 891
Hpy166II GTNNAC 4 cut(s) 360, 605, 889, 1243
Hpy188I TCNGA 9 cut(s) 217, 247, 334, 368, 380, 805, 944, 1566, 1589
Hpy188III TCNNGA 6 cut(s) 632, 734, 919, 1132, 1438, 1519
Hpy8I GTNNAC 4 cut(s) 360, 605, 889, 1243
Hpy99I CGWCG 3 cut(s) 254, 257, 1088
HpyAV CCTTC 2 cut(s) 1270, 1387
HpyCH4III ACNGT 2 cut(s) 364, 886
HpyCH4IV ACGT 4 cut(s) 545, 815, 896, 1313
HpyCH4V TGCA 7 cut(s) 183, 442, 747, 798, 983, 1022, 1652
HpyF3I CTNAG 4 cut(s) 188, 1026, 1133, 1254
HpySE526I ACGT 4 cut(s) 545, 815, 896, 1313
Hsp92II CATG 6 cut(s) 142, 229, 589, 977, 1225, 1660
HspAI GCGC 1 cut(s) 668
KroI GCCGGC 2 cut(s) 116, 1283
KroNI GCCGGC 2 cut(s) 118, 1285
Ksp22I TGATCA 1 cut(s) 375
KspI CCGCGG 1 cut(s) 758
Kzo9I GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
LmnI GCTCC 6 cut(s) 133, 303, 575, 851, 1035, 1481
LweI GCATC 3 cut(s) 223, 935, 1031
MaeI CTAG 4 cut(s) 23, 992, 1638, 1643
MaeII ACGT 4 cut(s) 545, 815, 896, 1313
MaeIII GTNAC 4 cut(s) 644, 897, 1319, 1418
MalI GATC 8 cut(s) 316, 353, 377, 753, 783, 1074, 1332, 1523
MboI GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
MboII GAAGA 2 cut(s) 34, 242
MfeI CAATTG 2 cut(s) 764, 1464
MflI RGATCY 2 cut(s) 751, 781
MhlI GDGCHC 3 cut(s) 138, 701, 821
MlyI GAGTC 6 cut(s) 66, 236, 365, 515, 713, 1555
MmeI TCCRAC 1 cut(s) 873
MroNI GCCGGC 2 cut(s) 116, 1283
MroXI GAANNNNTTC 1 cut(s) 1511
MseI TTAA 3 cut(s) 626, 651, 1374
MspA1I CMGCKG 2 cut(s) 127, 757
MspR9I CCNGG 2 cut(s) 779, 1547
MunI CAATTG 2 cut(s) 764, 1464
MvaI CCWGG 1 cut(s) 1547
MvnI CGCG 2 cut(s) 757, 1088
NaeI GCCGGC 2 cut(s) 118, 1285
NciI CCSGG 1 cut(s) 779
NcoI CCATGG 1 cut(s) 225
NdeII GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
NgoMIV GCCGGC 2 cut(s) 116, 1283
NlaIII CATG 6 cut(s) 142, 229, 589, 977, 1225, 1660
NlaIV GGNNCC 8 cut(s) 571, 659, 699, 753, 783, 1282, 1309, 1413
NmeAIII GCCGAG 1 cut(s) 546
NmuCI GTSAC 2 cut(s) 644, 897
PcsI WCGNNNNNNNCGW 3 cut(s) 246, 573, 684
PdiI GCCGGC 2 cut(s) 118, 1285
PdmI GAANNNNTTC 1 cut(s) 1511
PfeI GAWTC 3 cut(s) 218, 402, 1301
PleI GAGTC 6 cut(s) 66, 236, 364, 514, 713, 1555
PpsI GAGTC 6 cut(s) 66, 236, 364, 514, 713, 1555
Psp6I CCWGG 1 cut(s) 1545
PspGI CCWGG 1 cut(s) 1545
PspN4I GGNNCC 8 cut(s) 571, 659, 699, 753, 783, 1282, 1309, 1413
PspOMI GGGCCC 1 cut(s) 697
PspPI GGNCC 4 cut(s) 658, 697, 698, 1007
PstI CTGCAG 3 cut(s) 185, 444, 985
PsuI RGATCY 2 cut(s) 751, 781
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 4 cut(s) 581, 604, 836, 1043
RsaNI GTAC 4 cut(s) 580, 603, 835, 1042
SacII CCGCGG 1 cut(s) 758
SaqAI TTAA 3 cut(s) 626, 651, 1374
Sau3AI GATC 8 cut(s) 314, 351, 375, 751, 781, 1072, 1330, 1521
Sau96I GGNCC 4 cut(s) 658, 697, 698, 1007
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 6 cut(s) 66, 236, 365, 515, 713, 1555
ScrFI CCNGG 2 cut(s) 779, 1547
SduI GDGCHC 3 cut(s) 138, 701, 821
SfaNI GCATC 3 cut(s) 223, 935, 1031
SfcI CTRYAG 3 cut(s) 181, 440, 981
Sfr303I CCGCGG 1 cut(s) 758
SgrBI CCGCGG 1 cut(s) 758
SinI GGWCC 1 cut(s) 658
SspMI CTAG 4 cut(s) 23, 992, 1638, 1643
StyD4I CCNGG 2 cut(s) 777, 1545
StyI CCWWGG 2 cut(s) 225, 1161
TaaI ACNGT 2 cut(s) 364, 886
TaiI ACGT 4 cut(s) 548, 818, 899, 1316
TaqI TCGA 7 cut(s) 354, 633, 687, 873, 1075, 1437, 1524
TatI WGTACW 1 cut(s) 579
TauI GCSGC 2 cut(s) 263, 497
TfiI GAWTC 3 cut(s) 218, 402, 1301
Tru1I TTAA 3 cut(s) 626, 651, 1374
Tru9I TTAA 3 cut(s) 626, 651, 1374
TscAI CASTG 3 cut(s) 181, 643, 649
TseFI GTSAC 2 cut(s) 644, 897
Tsp45I GTSAC 2 cut(s) 644, 897
TspDTI ATGAA 7 cut(s) 308, 574, 586, 962, 1504, 1601, 1673
TspGWI ACGGA 3 cut(s) 337, 852, 1059
TspRI CASTG 3 cut(s) 181, 643, 649
VpaK11BI GGWCC 1 cut(s) 658
XapI RAATTY 2 cut(s) 381, 1606
XmnI GAANNNNTTC 1 cut(s) 1511
XspI CTAG 4 cut(s) 23, 992, 1638, 1643
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.