pycom09g06860

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0014476
Physical Location & Seq
Forward (+)
5077871 .. 5079994
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g06860.1

Sequence Viewer

Length: 1911 bp
ATGAAGAAGACCAGCTTTAAATTGAAGTTTTTTGCTTCTGGGTTCCCTTCTTCGTTTTCATTTTTTTTTTCCATCGGAATTCAAGTTCCATCTGGTTTTTCCGTTTCTGGGATTTTCAGATTTTCATCCAAAGATTTGGGCTTTCAGCGAAGTTTAAATCTTTCTTGGTTTTTCTCCAGTTTTTTTCTTCTCGAGTTTTATTCATGCAGGAATTTGAAACGAGAAGATACCATCAGAATGGCTGTTAAGGCTTCAATCCTACTAGTTCTCCTTGTGATCTTCTCCTCCACTTTAGCAGCGATTGCCAGAGTTCACAACCACCGCAATCACAACTCGAATGCCTCCACTTTCGCCGGCATACAACTGCCGGAGCACATGAGCTTCAACGCCGTCTCCTCCTCATCCCACACCGGCTGCAGCCTCAGCTGCTCTAAAATACCCAAGCAAGCAGACTCAACCATGGAAGAAGCTGAAGACTCCGACACCGACGAAGCAGCACCCGAATTGAACCCCCATAAACAGACTATGAAGCTCCACCTACGGCACAGATCACAGAACAAACAATCCGAAAGAAAAAGTTCAGTGATCGAGTCCACAGTCCGAGACTTGGTCAGAATTCAAACCCTCCACACAAGAATCGTTGAGAAGAAGAATCAGAACACATTTTCAAGGCTGCAGAAAGATAAGAAACCGAAGCACCACCAATCAAATCAGGTTGTAGCCCCGGCAGCCTCGCCGGAGTCCTACACCAACGAGCTCTCGGGCCAGCTTCAGGCCACGTTGAAGTCCGGCGTCAGCCTCGGCTCCGGCGAGTACTTCATGGATGTTTTCATCGGTACACCCCCTAAACAATTCTCTTTAATTCTCGACACTGGCAGTGACCTTAACTGGGTCCAATGCGTGCCGTGCCGCGACTGCTTCGAGCAGCACGGCCCGCACTACGACCCCAAAGACTCAACTTCTTTTCGTGACATTAGCTGCAAGAATCGGCGGTGTCAATTGGTTTCGTCCCCGGATCCTCCGCAACCCTGCAAGTCCGAAAATCAAACGTGTCCGTATTTCTACTGGTACGGAGACAGCTCCAACACCACCGGCGACTTAGCCCTCGAAACCTTTACCGTCAACCTCACGTCATCGAACAAGGCGGAGTTCAAGAGGGTGGAGAATGTGATGTTCGGATGCGGCCATTGGAACAGAGGATTGTTCCATGGGGCTGCAGGGTTGCTAGGTCTTGGCAGAGGTCCTCTTTCCTTTGCTTCTCAGCTCCAATCGCTGTACGGCCACTCGTTTTCCTACTGCCTTGTGGATCGGAACAGCGACGCGAATGTCAGCAGCAAGTTGATTTTTGGGGAGGACAAGAACCTCTTAAGCCACCCGAAACTGACCTACACTTCTTTGGTAGGAGGGAAAGAAAACCCTGCTGATACATTCTACTACGTCGAGTTAAAATCCGTCATGGTCGGCGGAGAAGCGGTGGACATACCGGCTGAGACTTGGAAATTGTCGCCGGAGGGTGCCGGTGGGACAATCATTGATTCCGGCACCACGTTGAGTTACTTTGCGGATCCAGCCTATCAAATTATCAAGGAGGCTTTTGAGAAAAAGGTCAAAGAGTATCCAGTCGTCAAGGACTTCCCATTTTTGGAACCTTGTTACAATGTGTCAGGTGTTGAGAAAATCGAGCTGCCGGAATTTGCAATTGTGTTTGCGGACGGAGCTGTGTGGGATTTCCCAGTTGAGAACTACTTCATCCAGATCGAACCGCAGGAAATCGTCTGCCTGGCGGTGTTGGGGACTCCGAAATCCGGCCTTTCGATCATCGGTAATTATCAGCAGCAGAATTTTCATATCCTCTATGATACAAAGAAGTCTAGGTTAGGATATGCACCCATGAAATGTGCTGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18A TAXi_C TAXi_N

Protein Analysis

637

Amino Acids

70.9

Weight (kDa)

7.24

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 271 - 630 4e-23 Eukaryotic aspartyl protease
TAXi_N PF14543 272 - 450 2.3e-53 Xylanase inhibitor N-terminal
TAXi_C PF14541 478 - 629 1e-35 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59080 AT3G59080
fragaria_vesca FvH4_6g38620
malus_domestica MD09G1149300.v1.1 MD17G1135600.v1.1
prunus_persica Prupe.3G177100_v2.0.a1
pyrus_communis pycom09g06860 pycom17g12940
rosa_chinensis RchiOBHm_Chr2g0152031
rosa_laevigata RLG00000020602
rosa_roxburghii Rroxscaffold_2G00096030
rosa_rugosa Rorug02G0430400
rosa_samantha Rh2AG491200 Rh2BG503500 Rh2CG477600 Rh2DG514800
rosa_wichuraiana Rw2G040380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1325
AccB1I GGYRCC 2 cut(s) 1514, 1541
AccII CGCG 2 cut(s) 912, 1322
AclWI GGATC 5 cut(s) 1010, 1023, 1314, 1559, 1572
AcoI YGGCCR 2 cut(s) 1183, 1279
AcsI RAATTY 5 cut(s) 78, 211, 615, 1691, 1840
AcuI CTGAAG 2 cut(s) 492, 755
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 4 cut(s) 815, 838, 1070, 1277
AfiI CCNNNNNNNGG 6 cut(s) 108, 607, 772, 889, 1642, 1805
AflII CTTAAG 1 cut(s) 1366
AflIII ACRYGT 1 cut(s) 1049
AhlI ACTAGT 1 cut(s) 262
AjiI CACGTC 1 cut(s) 1131
AjnI CCWGG 1 cut(s) 1779
AloI GAACNNNNNNTCC 4 cut(s) 69, 101, 548, 580
Alw21I GWGCWC 2 cut(s) 375, 759
Alw26I GTCTC 4 cut(s) 397, 597, 1068, 1484
AlwI GGATC 5 cut(s) 1010, 1023, 1314, 1559, 1572
Ama87I CYCGRG 2 cut(s) 191, 760
AoxI GGCC 6 cut(s) 763, 774, 931, 1183, 1279, 1807
ApoI RAATTY 5 cut(s) 78, 211, 615, 1691, 1840
Asp700I GAANNNNTTC 2 cut(s) 577, 1745
AspS9I GGNCC 4 cut(s) 763, 892, 932, 1241
AsuC2I CCSGG 2 cut(s) 725, 1013
AvaI CYCGRG 2 cut(s) 191, 760
AvaII GGWCC 2 cut(s) 892, 1241
BamHI GGATCC 2 cut(s) 1015, 1564
BanI GGYRCC 2 cut(s) 1514, 1541
BanII GRGCYC 1 cut(s) 759
BbsI GAAGAC 2 cut(s) 14, 480
Bbv12I GWGCWC 2 cut(s) 375, 759
BbvCI CCTCAGC 1 cut(s) 422
BccI CCATC 3 cut(s) 80, 97, 239
BceAI ACGGC 5 cut(s) 374, 557, 889, 946, 1294
BciT130I CCWGG 1 cut(s) 1781
BciVI GTATCC 1 cut(s) 1626
BcnI CCSGG 2 cut(s) 725, 1013
BcoDI GTCTC 4 cut(s) 397, 597, 1068, 1484
BcuI ACTAGT 1 cut(s) 262
BfaI CTAG 3 cut(s) 263, 1226, 1872
BfmI CTRYAG 3 cut(s) 415, 674, 1215
BfrI CTTAAG 1 cut(s) 1366
BfuI GTATCC 1 cut(s) 1626
BmcAI AGTACT 1 cut(s) 815
Bme1390I CCNGG 3 cut(s) 725, 1013, 1781
Bme18I GGWCC 2 cut(s) 892, 1241
BmeT110I CYCGRG 2 cut(s) 191, 760
BmgBI CACGTC 1 cut(s) 1131
BmgT120I GGNCC 4 cut(s) 763, 892, 932, 1241
BmiI GGNNCC 8 cut(s) 44, 805, 893, 1017, 1516, 1543, 1566, 1647
BmrFI CCNGG 3 cut(s) 725, 1013, 1781
BmrI ACTGGG 2 cut(s) 898, 1727
BmsI GCATC 1 cut(s) 1169
BmuI ACTGGG 2 cut(s) 898, 1727
BpiI GAAGAC 2 cut(s) 14, 480
BpmI CTGGAG 1 cut(s) 160
Bpu10I CCTNAGC 1 cut(s) 422
BpuMI CCSGG 2 cut(s) 725, 1013
BsaBI GATNNNNATC 1 cut(s) 124
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 5 cut(s) 459, 723, 799, 1011, 1207
BsaXI ACNNNNNCTCC 6 cut(s) 252, 282, 377, 407, 1458, 1488
Bsc4I CCNNNNNNNGG 6 cut(s) 108, 607, 772, 889, 1642, 1805
Bse118I RCCGGY 5 cut(s) 353, 410, 1091, 1483, 1517
Bse1I ACTGG 6 cut(s) 177, 877, 893, 1070, 1619, 1733
Bse8I GATNNNNATC 1 cut(s) 124
BseBI CCWGG 1 cut(s) 1781
BseDI CCNNGG 5 cut(s) 459, 723, 799, 1011, 1207
BseGI GGATG 5 cut(s) 125, 401, 829, 1184, 1749
BseJI GATNNNNATC 1 cut(s) 124
BseLI CCNNNNNNNGG 6 cut(s) 108, 607, 772, 889, 1642, 1805
BseMII CTCAG 3 cut(s) 436, 1274, 1479
BseNI ACTGG 6 cut(s) 177, 877, 893, 1070, 1619, 1733
BseRI GAGGAG 3 cut(s) 274, 385, 388
Bsh1236I CGCG 2 cut(s) 912, 1322
BshFI GGCC 6 cut(s) 765, 776, 933, 1185, 1281, 1809
BshNI GGYRCC 2 cut(s) 1514, 1541
BsiHKAI GWGCWC 2 cut(s) 375, 759
BsiHKCI CYCGRG 2 cut(s) 191, 760
BslFI GGGAC 3 cut(s) 994, 1537, 1807
BslI CCNNNNNNNGG 6 cut(s) 108, 607, 772, 889, 1642, 1805
BsmAI GTCTC 4 cut(s) 397, 597, 1068, 1484
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 3 cut(s) 994, 1537, 1807
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 6 cut(s) 765, 776, 933, 1185, 1281, 1809
BsoBI CYCGRG 2 cut(s) 191, 760
Bsp1286I GDGCHC 2 cut(s) 375, 759
Bsp143I GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
Bsp19I CCATGG 2 cut(s) 459, 1207
BspANI GGCC 6 cut(s) 765, 776, 933, 1185, 1281, 1809
BspCNI CTCAG 3 cut(s) 435, 1273, 1480
BspFNI CGCG 2 cut(s) 912, 1322
BspLI GGNNCC 8 cut(s) 44, 805, 893, 1017, 1516, 1543, 1566, 1647
BspMAI CTGCAG 3 cut(s) 419, 678, 1219
BspPI GGATC 5 cut(s) 1010, 1023, 1314, 1559, 1572
BspT107I GGYRCC 2 cut(s) 1514, 1541
BspTI CTTAAG 1 cut(s) 1366
BsrFI RCCGGY 5 cut(s) 353, 410, 1091, 1483, 1517
BsrI ACTGG 6 cut(s) 177, 877, 893, 1070, 1619, 1733
BssAI RCCGGY 5 cut(s) 353, 410, 1091, 1483, 1517
BssECI CCNNGG 5 cut(s) 459, 723, 799, 1011, 1207
BssMI GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
BssNI GRCGYC 1 cut(s) 792
BssT1I CCWWGG 2 cut(s) 459, 1207
Bst2UI CCWGG 1 cut(s) 1781
Bst4CI ACNGT 2 cut(s) 598, 1120
BstACI GRCGYC 1 cut(s) 792
BstAFI CTTAAG 1 cut(s) 1366
BstAPI GCANNNNNTGC 1 cut(s) 302
BstC8I GCNNGC 5 cut(s) 355, 447, 767, 902, 935
BstDEI CTNAG 4 cut(s) 422, 1099, 1260, 1488
BstDSI CCRYGG 2 cut(s) 459, 1207
BstF5I GGATG 5 cut(s) 125, 401, 829, 1184, 1749
BstFNI CGCG 2 cut(s) 912, 1322
BstKTI GATC 8 cut(s) 279, 551, 588, 1018, 1309, 1567, 1758, 1818
BstMAI GTCTC 4 cut(s) 397, 597, 1068, 1484
BstMBI GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
BstNI CCWGG 1 cut(s) 1781
BstSCI CCNGG 3 cut(s) 723, 1011, 1779
BstSFI CTRYAG 3 cut(s) 415, 674, 1215
BstUI CGCG 2 cut(s) 912, 1322
BstV2I GAAGAC 2 cut(s) 14, 480
BstX2I RGATCY 2 cut(s) 1015, 1564
BstXI CCANNNNNNTGG 2 cut(s) 136, 238
BstYI RGATCY 2 cut(s) 1015, 1564
BsuI GTATCC 1 cut(s) 1626
BsuRI GGCC 6 cut(s) 765, 776, 933, 1185, 1281, 1809
BtgI CCRYGG 2 cut(s) 459, 1207
BtrI CACGTC 1 cut(s) 1131
BtsCI GGATG 5 cut(s) 125, 401, 829, 1184, 1749
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 3 cut(s) 588, 870, 883
Cac8I GCNNGC 5 cut(s) 355, 447, 767, 902, 935
Cfr10I RCCGGY 5 cut(s) 353, 410, 1091, 1483, 1517
Cfr13I GGNCC 4 cut(s) 763, 892, 932, 1241
CseI GACGC 2 cut(s) 781, 1328
Csp6I GTAC 4 cut(s) 814, 837, 1069, 1276
CviAII CATG 7 cut(s) 204, 376, 460, 820, 1208, 1456, 1891
CviQI GTAC 4 cut(s) 814, 837, 1069, 1276
DdeI CTNAG 4 cut(s) 422, 1099, 1260, 1488
DpnI GATC 8 cut(s) 278, 550, 587, 1017, 1308, 1566, 1757, 1817
DpnII GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
DraI TTTAAA 2 cut(s) 19, 156
DrdI GACNNNNNNGTC 1 cut(s) 1325
DseDI GACNNNNNNGTC 1 cut(s) 1325
EaeI YGGCCR 2 cut(s) 1183, 1279
EciI GGCGGA 2 cut(s) 1160, 1479
Ecl136II GAGCTC 1 cut(s) 757
Eco130I CCWWGG 2 cut(s) 459, 1207
Eco24I GRGCYC 1 cut(s) 759
Eco47I GGWCC 2 cut(s) 892, 1241
Eco53kI GAGCTC 1 cut(s) 757
Eco57I CTGAAG 2 cut(s) 492, 755
Eco88I CYCGRG 2 cut(s) 191, 760
EcoICRI GAGCTC 1 cut(s) 757
EcoO109I RGGNCCY 1 cut(s) 1241
EcoRI GAATTC 2 cut(s) 78, 615
EcoRII CCWGG 1 cut(s) 1779
EcoT14I CCWWGG 2 cut(s) 459, 1207
EcoT38I GRGCYC 1 cut(s) 759
ErhI CCWWGG 2 cut(s) 459, 1207
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 7 cut(s) 207, 379, 463, 823, 1211, 1459, 1894
FalI AAGNNNNNCTT 3 cut(s) 31, 1349, 1381
FaqI GGGAC 3 cut(s) 994, 1537, 1807
FatI CATG 7 cut(s) 203, 375, 459, 819, 1207, 1455, 1890
FauI CCCGC 1 cut(s) 942
FokI GGATG 5 cut(s) 112, 388, 836, 1191, 1736
FriOI GRGCYC 1 cut(s) 759
FspBI CTAG 3 cut(s) 263, 1226, 1872
GsuI CTGGAG 1 cut(s) 160
HaeIII GGCC 6 cut(s) 765, 776, 933, 1185, 1281, 1809
HgaI GACGC 2 cut(s) 781, 1328
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 7 cut(s) 207, 379, 463, 823, 1211, 1459, 1894
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
Hpy166II GTNNAC 5 cut(s) 313, 594, 839, 1123, 1477
Hpy188III TCNNGA 5 cut(s) 191, 866, 968, 1153, 1753
Hpy8I GTNNAC 5 cut(s) 313, 594, 839, 1123, 1477
Hpy99I CGWCG 3 cut(s) 491, 1322, 1442
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 598, 1120
HpyCH4IV ACGT 5 cut(s) 779, 1049, 1130, 1437, 1547
HpyCH4V TGCA 8 cut(s) 207, 417, 676, 981, 1032, 1217, 1697, 1886
HpyF3I CTNAG 4 cut(s) 422, 1099, 1260, 1488
HpySE526I ACGT 5 cut(s) 779, 1049, 1130, 1437, 1547
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 7 cut(s) 207, 379, 463, 823, 1211, 1459, 1894
KroI GCCGGC 1 cut(s) 353
KroNI GCCGGC 1 cut(s) 355
Kzo9I GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
LmnI GCTCC 6 cut(s) 370, 537, 809, 1085, 1269, 1715
LweI GCATC 1 cut(s) 1169
MaeI CTAG 3 cut(s) 263, 1226, 1872
MaeII ACGT 5 cut(s) 779, 1049, 1130, 1437, 1547
MaeIII GTNAC 4 cut(s) 878, 968, 1553, 1652
MalI GATC 8 cut(s) 278, 550, 587, 1017, 1308, 1566, 1757, 1817
MboI GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
MfeI CAATTG 2 cut(s) 998, 1698
MflI RGATCY 2 cut(s) 1015, 1564
MhlI GDGCHC 2 cut(s) 375, 759
MlyI GAGTC 6 cut(s) 446, 470, 599, 749, 947, 1789
MmeI TCCRAC 2 cut(s) 504, 1107
MroNI GCCGGC 1 cut(s) 353
MroXI GAANNNNTTC 2 cut(s) 577, 1745
MseI TTAA 7 cut(s) 18, 155, 246, 860, 885, 1367, 1445
MslI CAYNNNNRTG 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 426
MspCI CTTAAG 1 cut(s) 1366
MspR9I CCNGG 3 cut(s) 725, 1013, 1781
MunI CAATTG 2 cut(s) 998, 1698
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 1 cut(s) 1781
MvnI CGCG 2 cut(s) 912, 1322
NaeI GCCGGC 1 cut(s) 355
NciI CCSGG 2 cut(s) 725, 1013
NcoI CCATGG 2 cut(s) 459, 1207
NdeII GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
NgoMIV GCCGGC 1 cut(s) 353
NlaIII CATG 7 cut(s) 207, 379, 463, 823, 1211, 1459, 1894
NlaIV GGNNCC 8 cut(s) 44, 805, 893, 1017, 1516, 1543, 1566, 1647
NmeAIII GCCGAG 1 cut(s) 780
NmuCI GTSAC 2 cut(s) 878, 968
PaeR7I CTCGAG 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 807
PctI GAATGC 1 cut(s) 343
PdiI GCCGGC 1 cut(s) 355
PdmI GAANNNNTTC 2 cut(s) 577, 1745
PfeI GAWTC 4 cut(s) 636, 652, 985, 1535
PflFI GACNNNGTC 1 cut(s) 608
PleI GAGTC 6 cut(s) 446, 470, 598, 748, 947, 1789
PpsI GAGTC 6 cut(s) 446, 470, 598, 748, 947, 1789
PpuMI RGGWCCY 1 cut(s) 1241
Psp124BI GAGCTC 1 cut(s) 759
Psp5II RGGWCCY 1 cut(s) 1241
Psp6I CCWGG 1 cut(s) 1779
PspGI CCWGG 1 cut(s) 1779
PspN4I GGNNCC 8 cut(s) 44, 805, 893, 1017, 1516, 1543, 1566, 1647
PspPI GGNCC 4 cut(s) 763, 892, 932, 1241
PspPPI RGGWCCY 1 cut(s) 1241
PstI CTGCAG 3 cut(s) 419, 678, 1219
PsuI RGATCY 2 cut(s) 1015, 1564
PsyI GACNNNGTC 1 cut(s) 608
PvuII CAGCTG 1 cut(s) 426
RsaI GTAC 4 cut(s) 815, 838, 1070, 1277
RsaNI GTAC 4 cut(s) 814, 837, 1069, 1276
RseI CAYNNNNRTG 1 cut(s) 236
SacI GAGCTC 1 cut(s) 759
SaqAI TTAA 7 cut(s) 18, 155, 246, 860, 885, 1367, 1445
Sau3AI GATC 8 cut(s) 276, 548, 585, 1015, 1306, 1564, 1755, 1815
Sau96I GGNCC 4 cut(s) 763, 892, 932, 1241
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 6 cut(s) 446, 470, 599, 749, 947, 1789
ScrFI CCNGG 3 cut(s) 725, 1013, 1781
SduI GDGCHC 2 cut(s) 375, 759
SfaNI GCATC 1 cut(s) 1169
SfcI CTRYAG 3 cut(s) 415, 674, 1215
Sfr274I CTCGAG 1 cut(s) 191
SgrAI CRCCGGYG 1 cut(s) 1091
SinI GGWCC 2 cut(s) 892, 1241
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 191, 1366
SmoI CTYRAG 2 cut(s) 191, 1366
SpeI ACTAGT 1 cut(s) 262
SspMI CTAG 3 cut(s) 263, 1226, 1872
SstI GAGCTC 1 cut(s) 759
StyD4I CCNGG 3 cut(s) 723, 1011, 1779
StyI CCWWGG 2 cut(s) 459, 1207
TaaI ACNGT 2 cut(s) 598, 1120
TaiI ACGT 5 cut(s) 782, 1052, 1133, 1440, 1550
TatI WGTACW 1 cut(s) 813
TauI GCSGC 2 cut(s) 912, 1185
TfiI GAWTC 4 cut(s) 636, 652, 985, 1535
Tru1I TTAA 7 cut(s) 18, 155, 246, 860, 885, 1367, 1445
Tru9I TTAA 7 cut(s) 18, 155, 246, 860, 885, 1367, 1445
TscAI CASTG 3 cut(s) 588, 877, 883
TseFI GTSAC 2 cut(s) 878, 968
Tsp45I GTSAC 2 cut(s) 878, 968
TspGWI ACGGA 5 cut(s) 91, 1044, 1086, 1441, 1728
TspRI CASTG 3 cut(s) 588, 877, 883
Tth111I GACNNNGTC 1 cut(s) 608
Vha464I CTTAAG 1 cut(s) 1366
VpaK11BI GGWCC 2 cut(s) 892, 1241
XapI RAATTY 5 cut(s) 78, 211, 615, 1691, 1840
XhoI CTCGAG 1 cut(s) 191
XmnI GAANNNNTTC 2 cut(s) 577, 1745
XspI CTAG 3 cut(s) 263, 1226, 1872
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.